American journal of veterinary research2022; 83(9); ajvr.22.02.0025; doi: 10.2460/ajvr.22.02.0025

Plasma ascorbic acid, antioxidant capacity, and reactive oxygen species in healthy foals.

Abstract: To describe ascorbic acid (AA) concentrations, plasma antioxidant capacity (PAC) and markers of oxidative stress, as measured by derivatives of reactive oxygen metabolites (dROMs), in healthy foals at birth and during the first month of life. Methods: Venous blood samples were collected from healthy Standardbred (n = 13) and Quarter Horse (n = 10) foals. Plasma AA, PAC, and dROMs were assessed at 3 to 12 hours, 3 days, and 1, 2, and 4 weeks of age. Methods: AA was measured via high-performance liquid chromatography. PAC and dROMs were measured with a free radical analytical system. Comparisons of AA, PAC, and dROMs at different time points were assessed. Results: Mean ± standard deviation AA concentrations at 3 to 12 hours (44.7 ± 19.6 μmol/L; P ≤ .01), 1 (48.6 ± 22.5 μmol/L; P ≤ .001), and 2 weeks (41.8 ± 15.8 μmol/L; P ≤ .001) were higher than at 4 weeks of age (28.5 ± 12.7 μmol/L). Both PAC and dROMs significantly increased at different time points compared to 3 to 12 hours of age. Conclusions: Healthy foals have higher plasma AA concentrations shortly after birth, which then gradually decrease throughout the first month of life, suggesting that AA may represent a key antioxidant in the postnatal period. The concurrent increase in PAC and dROMs suggests that dynamic development of oxidative balance occurs after birth in foals. Development of AA, PAC, and dROM reference ranges in healthy foals could be used to guide therapeutic interventions and monitor during disease states characterized by increased oxidative stress.
Publication Date: 2022-07-13 PubMed ID: 35895767DOI: 10.2460/ajvr.22.02.0025Google Scholar: Lookup
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Summary

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The research article studies the concentration of ascorbic acid (AA), plasma antioxidant capacity (PAC), and the levels of oxidative stress in healthy newborn horses over the first month of life. It finds that the plasma ascorbic acid concentration and oxidative balance varies significantly during this period, suggesting that ascorbic acid plays a vital antioxidant role after birth.

Methodology

  • The study utilized blood samples taken from healthy Standardbred and Quarter Horse foals. These blood samples were then segregated into groups and tested at specific time intervals – 3 to 12 hours, 3 days, and 1, 2, and 4 weeks after birth.
  • Ascorbic acid levels were determined using high-performance liquid chromatography, a technique used to identify and quantify components in a sample.
  • Levels of plasma antioxidant capacity (PAC) and derivatives of reactive oxygen metabolites (dROMs) – markers of oxidative stress – were measured using a free radical analytical system.
  • A comparative analysis was performed on the measured levels of AA, PAC, and dROMs at these different time points.

Results

  • The results showed that the concentrations of AA were significantly higher at 3 to 12 hours, 1 week, and 2 weeks after birth, compared to the concentrations measured at 4 weeks of age.
  • Similarly, the levels of both PAC and dROMs also showed significant increases at different times compared to the foal’s first 3 to 12 hours of age.

Conclusions

  • The study concluded that healthy foals exhibit higher plasma AA concentrations shortly after birth, which then gradually decrease over the first month of their life. This indicates that AA might be a key antioxidant during the postnatal period.
  • The simultaneous increase in PAC and dROMs over time suggests that a dynamic development of oxidative balance occurs after birth in foals.
  • The research suggests that a developmental timeline of AA, PAC, and dROM levels in healthy foals could be used to inform therapeutic interventions and monitor disease states characterized by increased oxidative stress.

Cite This Article

APA
Migliorisi A, Hart K, Vaughn S, Austin S, Aldridge B, Wilkins P. (2022). Plasma ascorbic acid, antioxidant capacity, and reactive oxygen species in healthy foals. Am J Vet Res, 83(9), ajvr.22.02.0025. https://doi.org/10.2460/ajvr.22.02.0025

Publication

ISSN: 1943-5681
NlmUniqueID: 0375011
Country: United States
Language: English
Volume: 83
Issue: 9
PII: ajvr.22.02.0025

Researcher Affiliations

Migliorisi, Alessandro
  • Department of Clinical Sciences, Colorado State University, Fort Collins, CO.
Hart, Kelsey
  • Department of Large Animal Medicine, College of Veterinary Medicine, University of Georgia, Athens, GA.
Vaughn, Sarah
  • Department of Large Animal Medicine, College of Veterinary Medicine, University of Georgia, Athens, GA.
Austin, Scott
  • Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois Urbana-Champaign, Urbana, IL.
Aldridge, Brian
  • Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois Urbana-Champaign, Urbana, IL.
Wilkins, Pamela
  • Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois Urbana-Champaign, Urbana, IL.

MeSH Terms

  • Animals
  • Animals, Newborn
  • Antioxidants
  • Ascorbic Acid
  • Horses
  • Oxidative Stress
  • Reactive Oxygen Species

Citations

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