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Home / Equine Research Bank / Front Vet Sci / Platelet and Leukocyte Concentration in Equine Autologous Co...
Frontiers in veterinary science2020; 7; 173; doi: 10.3389/fvets.2020.00173

Platelet and Leukocyte Concentration in Equine Autologous Conditioned Plasma Are Inversely Distributed by Layer and Are Not Affected by Centrifugation Rate.

Abstract: Platelet rich plasma (PRP) is used extensively in equine regenerative medicine. Differences in preparation protocols give rise to significant variability in the cellular composition of PRP making it very difficult to establish a standard of care in the field. This study aimed to optimize the preparation protocol for leukocyte-reduced PRP (P-PRP). Blood (100 mL) was collected from horses ( = 5) and divided into 2 purple top EDTA tubes and 6 (15 mL) double syringes with a final concentration of 10% acid citrate dextrose anticoagulant. Six double syringes were collected from each horse; PRP samples were prepared in duplicate and centrifuged at 1,100 rpm (188 × g), 1,300 rpm (263 × g), or 1,500 rpm (350 × g). Duplicates were subjected to +/- braking at the end of centrifugation. The total volume of PRP generated was measured and divided into thirds. Each third (top, middle, and bottom) were drawn off separately using the inner (6 mL syringe) and placed in purple top EDTA tubes. Automated complete blood counts were performed on all peripheral whole blood and PRP samples. The concentration of leukocytes was higher in the bottom layer of PRP compared to the top and middle layers ( < 0.0001). The concentration of platelets was slightly lower in the bottom layer of PRP than the middle layer ( = 0.02). Centrifugation braking increased the leukocyte concentration in the top ( = 0.03) and middle layers of PRP ( = 0.001). Centrifugation rate had no effect on the cellular composition of PRP ( = 0.1-0.6). Because layer of plasma affected both platelet and leukocyte concentrations in PRP, the most important modification for the current single spin, double syringe, plasma based PRP preparation protocols is to exclude the bottom 1/3 layer of PRP.
Copyright © 2020 Radtke, Goodale and Fortier.
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Publication Date: 2020-05-12 PubMed ID: 32478101PubMed Central: PMC7235160DOI: 10.3389/fvets.2020.00173Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research is an investigation into the concentration of platelets and leukocytes in the blood plasma of horses, particularly in the context of Platelet rich plasma (PRP) treatment, a regenerative medicine therapy. The study also explores the influence of centrifugation rates on this concentration.

Introduction

  • This study focused on determining the levels of platelets and leukocytes distributed in the blood plasma of horses.
  • Platelet Rich Plasma (PRP) therapy is widely used in equine regenerative medicine. PRP is a volume of plasma that has an increased concentration of platelets.
  • Due to the differences in the ways PRP is prepared, there tends to be major differences in the cellular composition of PRP, which severely affects establishing a reliable standard for treatment methods.
  • The aim of this research was to optimize the protocols for preparing leukocyte-reduced PRP, also known as P-PRP.

Methodology

  • Blood was collected from 5 horses and divided into various tubes and syringes with specific anticoagulants.
  • The samples were then subjected to different centrifugation speeds, and additionally, the process was either braked or allowed naturally to finish.
  • The volume of PRP harvested was then measured and split into separate sections (top, middle, and bottom), with each measured separately to assess its cellular composition.

Findings

  • The researchers found that the concentration of leukocytes was higher in the bottom layer of PRP as compared to both the middle and top layers.
  • Interestingly, the concentration of platelets was found to be slightly lower in the bottom layer of PRP as compared to the middle layer.
  • They also found centrifugation braking increased the leukocyte concentration in the top and middle layers.
  • They discovered that the rate of centrifugation had no apparent effect on the cellular composition of PRP.

Conclusion

  • The study concluded that the most significant factor affecting the concentration of both platelets and leukocytes in PRP across the different layers is primarily the fact of centrifugation itself and, to a lesser extent, centrifugal braking.
  • Based on the findings, the researchers suggest amending the current PRP preparation procedures by excluding the bottom third layer of PRP, since it has a notably different cellular composition.

Cite This Article

APA
Radtke AV, Goodale MB, Fortier LA. (2020). Platelet and Leukocyte Concentration in Equine Autologous Conditioned Plasma Are Inversely Distributed by Layer and Are Not Affected by Centrifugation Rate. Front Vet Sci, 7, 173. https://doi.org/10.3389/fvets.2020.00173

Publication

Frontiers in veterinary science
ISSN: 2297-1769
NlmUniqueID: 101666658
Country: Switzerland
Language: English
Volume: 7
Pages: 173
PII: 173

Researcher Affiliations

Radtke, Alexandra V
  • Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY, United States.
Goodale, Margaret B
  • Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY, United States.
Fortier, Lisa A
  • Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY, United States.

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Citations

This article has been cited 5 times.
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