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Home / Equine Research Bank / Stem Cell Res Ther / Polymer-mineral scaffold augments in vivo equine multipotent...
Stem cell research & therapy2018; 9(1); 60; doi: 10.1186/s13287-018-0790-8

Polymer-mineral scaffold augments in vivo equine multipotent stromal cell osteogenesis.

Abstract: Use of bioscaffolds to direct osteogenic differentiation of adult multipotent stromal cells (MSCs) without exogenous proteins is a contemporary approach to bone regeneration. Identification of in vivo osteogenic contributions of exogenous MSCs on bioscaffolds after long-term implantation is vital to understanding cell persistence and effect duration. This study was designed to quantify in vivo equine MSC osteogenesis on synthetic polymer scaffolds with distinct mineral combinations 9 weeks after implantation in a murine model. Cryopreserved, passage (P)1, equine bone marrow-derived MSCs (BMSC) and adipose tissue-derived MSCs (ASC) were culture expanded to P3 and immunophenotyped with flow cytometry. They were then loaded by spinner flask on to scaffolds composed of tricalcium phosphate (TCP)/hydroxyapatite (HA) (40:60; HT), polyethylene glycol (PEG)/poly-L-lactic acid (PLLA) (60:40; GA), or PEG/PLLA/TCP/HA (36:24:24:16; GT). Scaffolds with and without cells were maintained in static culture for up to 21 days or implanted subcutaneously in athymic mice that were radiographed every 3 weeks up to 9 weeks. In vitro cell viability and proliferation were determined. Explant composition (double-stranded (ds)DNA, collagen, sulfated glycosaminoglycan (sGAG), protein), equine and murine osteogenic target gene expression, microcomputed tomography (μCT) mineralization, and light microscopic structure were assessed. The ASC and BMSC number increased significantly in HT constructs between 7 and 21 days of culture, and BMSCs increased similarly in GT constructs. Radiographic opacity increased with time in GT-BMSC constructs. Extracellular matrix (ECM) components and dsDNA increased significantly in GT compared to HT constructs. Equine and murine osteogenic gene expression was highest in BMSC constructs with mineral-containing scaffolds. The HT constructs with either cell type had the highest mineral deposition based on μCT. Regardless of composition, scaffolds with cells had more ECM than those without, and osteoid was apparent in all BMSC constructs. In this study, both exogenous and host MSCs appear to contribute to in vivo osteogenesis. Addition of mineral to polymer scaffolds enhances equine MSC osteogenesis over polymer alone, but pure mineral scaffold provides superior osteogenic support. These results emphasize the need for bioscaffolds that provide customized osteogenic direction of both exo- and endogenous MSCs for the best regenerative potential.
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Publication Date: 2018-03-09 PubMed ID: 29523214PubMed Central: PMC5845133DOI: 10.1186/s13287-018-0790-8Google Scholar: Lookup
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  • Journal Article
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research study investigates the osteogenesis or bone development potential of horse-derived multipotent stromal cells (MSCs) when combined with synthetic polymer scaffolds. The study further explores the effects of different mineral combinations in the scaffolds on cell proliferation and bone formation.

Objectives and Setup of the Research

  • The study aimed to quantify the osteogenesis capability of equine MSCs after being implanted on bioscaffolds with different mineral combinations in a murine (mouse) model for a period of 9 weeks.
  • The researchers used cryopreserved, equine bone marrow-derived MSCs (BMSC) and adipose tissue-derived MSCs (ASC) and put them through culture expansion.
  • The cells were then loaded onto scaffolds composed of different proportions of tricalcium phosphate (TCP), hydroxyapatite (HA), polyethylene glycol (PEG), and poly-L-lactic acid (PLLA).
  • The scaffolds with and without the cells were maintained in static culture for up to 21 days or implanted subcutaneously in athymic mice for a period of 9 weeks.

Results of the Experiment

  • The study found that the number of ASC and BMSC cells significantly increased in constructs with TCP/HA mineral combination during the 21-day culture period.
  • The same increase in BMSCs was observed in constructs where PEG/PLLA/TCP/HA combination was used.
  • An increase in radiographic opacity was also observed over time in constructs with the latter mineral combination filled with BMSCs, suggesting an enhanced osteogenesis or bone formation.
  • Extracellular matrix (ECM) components and double-stranded DNA showed significant increases in polymer scaffolds combined with mineral than in pure mineral constructs (TCP/HA).
  • Highest gene expression indicating bone formation was found in BMSC constructs where the scaffolds included minerals.
  • The pure mineral scaffolds with either cell type had the highest mineral deposition according to microcomputed tomography.
  • Regardless of the composition of the scaffold, those with cells had more ECM than those without, and osteoid or new bone was evident in all BMSC constructs.

Implications of the Research

  • The study reveals that both exogenous (externally applied) and host MSCs contribute to bone formation when implanted on synthetic polymer scaffolds.
  • Adding mineral to polymer scaffolds improves the potential for bone development by the MSCs compared to polymer alone.
  • However, scaffolds composed purely of mineral exhibited the best support for fostering bone development.
  • The results highlight the need for bioscaffolds that cater to the osteogenic requirements of both externally applied and naturally occurring MSCs for optimal regenerative outcomes.

Cite This Article

APA
Duan W, Chen C, Haque M, Hayes D, Lopez MJ. (2018). Polymer-mineral scaffold augments in vivo equine multipotent stromal cell osteogenesis. Stem Cell Res Ther, 9(1), 60. https://doi.org/10.1186/s13287-018-0790-8

Publication

Stem cell research & therapy
ISSN: 1757-6512
NlmUniqueID: 101527581
Country: England
Language: English
Volume: 9
Issue: 1
Pages: 60
PII: 60

Researcher Affiliations

Duan, Wei
  • Laboratory for Equine and Comparative Orthopedic Research, Louisiana State University, Baton Rouge, LA, USA.
Chen, Cong
  • Department of Biomedical Engineering, Pennsylvania State University, University Park, PA, USA.
Haque, Masudul
  • Laboratory for Equine and Comparative Orthopedic Research, Louisiana State University, Baton Rouge, LA, USA.
Hayes, Daniel
  • Department of Biomedical Engineering, Pennsylvania State University, University Park, PA, USA.
Lopez, Mandi J
  • Laboratory for Equine and Comparative Orthopedic Research, Louisiana State University, Baton Rouge, LA, USA. mlopez@lsu.edu.

MeSH Terms

  • Adipose Tissue / cytology
  • Animals
  • Cells, Cultured
  • Horses
  • Hydroxyapatites / chemistry
  • Lactates / chemistry
  • Male
  • Mesenchymal Stem Cell Transplantation / methods
  • Mesenchymal Stem Cells / cytology
  • Mice
  • Osteogenesis
  • Pluripotent Stem Cells / cytology
  • Polyethylene Glycols / chemistry
  • Tissue Scaffolds / chemistry

Conflict of Interest Statement

ETHICS APPROVAL: All animal procedures were approved by the Louisiana State University Institutional Animal Care and Use Committee prior to study initiation (protocols #13-050 and 07-049). CONSENT FOR PUBLICATION: Not applicable. COMPETING INTERESTS: The authors declare that they have no competing interests. PUBLISHER’S NOTE: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

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