Population of follicles and luteal structures during the oestrous cycle of mares detected by three-dimensional internal structure microscopy.
Abstract: The structure of the equine ovary is different from that of other mammals in its extremely large size, the presence of ovarian fossa and the inverted location of its cortex and medulla. A three-dimensional internal structure microscopy (3D-ISM), which consists of a computer-controlled slicer, a CCD camera, a laser disc recorder and a PC, is very useful for the observation of the internal structures in equine ovaries. In addition, the three-dimensional images of follicles and corpus luteum (CL) reconstructed by the segmentation technique can clarify the spatial arrangement in the equine ovary. In this study, to understand the changes in the ovarian internal structures of the mare during the oestrous cycle, the size and numbers of follicles and luteal structures were analysed by 3D-ISM in addition to the concentrations of progesterone (P(4)) and oestradiol-17beta. As a result, many small follicles ( 1 ng/ml. CHs were also observed in the group, which had low level of P(4) (P(4) < 1 ng/ml). CAs were found regardless of the P(4) level. In conclusion, 3D-ISM enabled the internal observation of the ovarian structures in detail, and estimation of the stage of the ovarian cycle with complementary physiological information. The findings by 3D-ISM provide basic information for clinical applications.
Publication Date: 2009-05-28 PubMed ID: 19469767DOI: 10.1111/j.1439-0264.2008.00924.xGoogle Scholar: Lookup
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- Journal Article
Summary
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The research article demonstrates the use of a novel imaging method, three-dimensional internal structure microscopy (3D-ISM), for observing the internal structures of a mare’s ovaries over the course of an oestrous cycle. This technique has made it possible to track changes in the numbers and sizes of follicles and luteal structures, and to correlate these data with hormone levels.
Study Methodology
- The researchers used 3D-ISM to observe the internal structures of equine ovaries. This advanced microscopy involves a computer-controlled slicer, a CCD camera, a laser disc recorder and a PC.
- The researchers focused on the follicles and the corpus luteum (CL), the latter being a transitory endocrine structure involved in the reproductive cycle of mares.
- This allowed them to utilise the segmentation technique to reconstruct three-dimensional images, shedding light on the spatial configuration inside the equine ovary.
Findings and Results
- The main discovery in this study was the presence of many small follicles of less than 10 mm in diameter.
- The researchers were also able to differentiate three types of luteal structures: the corpus haemorragicum (CH), which forms from blood elements in the cavity after ovulation, CL, and the corpus albican (CA).
- They found that the presence of CHs and CL correlated with higher concentrations of progesterone (P(4)) (>1 ng/ml), while CHs were also found at lower P(4) concentrations (<1ng/ml).
- However, CAs were discovered regardless of P(4) level.
Conclusion and Implications
- The findings of the study demonstrated that 3D-ISM allows for detailed observation of ovarian internal structures and aids in determining the stage of the ovarian cycle, supplemented by physiological information.
- These insights acquired through 3D-ISM offer fundamental knowledge that can be applied clinically, improving the understanding of the oestrous cycle in mares.
Cite This Article
APA
Hirano Y, Kimura J, Nambo Y, Yokota H, Nakamura S, Takemoto S, Himeno R, Mishima T, Matsui M, Miyake YI.
(2009).
Population of follicles and luteal structures during the oestrous cycle of mares detected by three-dimensional internal structure microscopy.
Anat Histol Embryol, 38(3), 214-218.
https://doi.org/10.1111/j.1439-0264.2008.00924.x Publication
Researcher Affiliations
- Department of Clinical Veterinary Science, The United Graduate School of Veterinary Sciences, Gifu University, Gifu, Japan.
MeSH Terms
- Animals
- Corpus Luteum / anatomy & histology
- Corpus Luteum / ultrastructure
- Estradiol / blood
- Estrus / blood
- Estrus / physiology
- Female
- Horses / anatomy & histology
- Horses / blood
- Horses / physiology
- Image Processing, Computer-Assisted
- Imaging, Three-Dimensional / veterinary
- Microscopy / instrumentation
- Microscopy / methods
- Microscopy / veterinary
- Ovarian Follicle / anatomy & histology
- Ovarian Follicle / ultrastructure
- Progesterone / blood
- Videodisc Recording
Citations
This article has been cited 1 times.- Ono M, Akuzawa H, Nambo Y, Hirano Y, Kimura J, Takemoto S, Nakamura S, Yokota H, Himeno R, Higuchi T, Ohtaki T, Tsumagari S. Analysis of the equine ovarian structure during the first twelve months of life by three-dimensional internal structure microscopy. J Vet Med Sci 2016 Jan;77(12):1599-603.
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