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Acta veterinaria Scandinavica2024; 66(1); 54; doi: 10.1186/s13028-024-00774-6

Predominance of enterotoxigenic Escherichia coli among ESBL/plasmid-mediated AmpC-producing strains isolated from diarrheic foals: a public health concern.

Abstract: The upsurge of diarrheagenic E. coli pathotypes carrying extended-spectrum beta-lactamases (ESBLs)/plasmid-mediated AmpC β-lactamase (pAmpC) among animals constitutes an emerging threat for humans and animals. This study investigated the burden of ESBL-/pAmpC-producing diarrheagenic E. coli among diarrheic foals and its potential public health implications. Rectal swabs were collected from 80 diarrheic foals. These swabs were processed to isolate and identify ESBL/pAmpC-producing E. coli using a selective culture medium, biochemical tests, phenotypic identification, and molecular identification of ESBL- and pAmpC-encoding genes. Moreover, all ESBL-/pAmpC-producing E. coli isolates were examined for different virulence genes related to diarrheagenic E. coli pathotypes. Results: Out of 80 examined foals, 26 (32.5%) were confirmed as ESBL-/pAmpC-producing E. coli, of which 14 (17.5%) animals carried only ESBL-producing E. coli, whereas 12 (15%) animals possessed ESBL-pAmpC-producing E. coli. The only detected diarrheagenic pathotype was enterotoxigenic, encoded by the heat-stable enterotoxin gene (ST) with a prevalence rate of 80.8% (21/26). The ST gene was further characterized where STa, STb, and STa + STb were found in one, four, and 16 strains, respectively. Moreover, all enterotoxigenic E. coli (ETEC) isolates exhibited a multidrug-resistance pattern. The phylogenetic analysis of 3 obtained partial STb sequences revealed high genetic relatedness to ETEC isolates retrieved from humans, conferring such sequences' public health significance. Conclusions: These findings highlight that diarrheic foals could serve as a potential reservoir for multidrug-resistant ESBL-/pAmpC-producing enterotoxigenic E. coli.
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Publication Date: 2024-10-03 PubMed ID: 39363309PubMed Central: PMC11448284DOI: 10.1186/s13028-024-00774-6Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article discusses the growing prevalence and public health concerns associated with a specific type of Escherichia coli (E. coli) found in diarrheic foals, which is resistant to certain antibiotics and can harm both animals and humans.

Research Overview

The primary focus of this study is on the burgeoning presence of diarrheagenic E. coli pathotypes, notably those carrying extended spectrum beta-lactamases (ESBLs) or plasmid-mediated AmpC Beta-lactamase (pAmpC), especially among animals.

  • The researchers collected rectal samples from 80 foals experiencing diarrhoea.
  • These samples were processed to identify and isolate ESBL/pAmpC-producing E. coli, which was achieved using selective culture moderating, biochemical tests, phenotypic and molecular identification of ESBL and pAmpC-encoding genes.

Research Findings

  • From the 80 foals that were examined, 26, which is 32.5%, were confirmed to have ESBL/pAmpC-producing E. coli. Specifically, it was found that 14 (17.5%) of the foals carried ESBL-producing E. coli only, whereas 12 (15%) foals had ESBL-pAmpC-producing E. coli.
  • The only discovered diarrheagenic pathotype was the enterotoxigenic E. coli (ETEC), identifiable by the heat-stable enterotoxin gene (ST), with an 80.8% prevalence rate.
  • All ETEC strains demonstrated a multi-drug-resistance pattern, causing concern for treatment difficulties.

Public Health Implications

  • Phylogenetic analysis indicated high genetic similarity between ETEC strains isolated from foals and humans. This suggested the potential risk that infection can spillover from animals to humans, thus accentuating the significance of this study to public health awareness.
  • As the study concludes, diarrheic foals could act as a potential reservoir for multidrug-resistant ESBL-/pAmpC-producing E. coli.

In summary, this study provides valuable insights into how ESBL-/pAmpC-producing E. coli spread among animals and humans which in turn can pave the way for newer, more effective prevention and control strategies.

Cite This Article

APA
Samir A, Abdel-Moein KA, Zaher HM. (2024). Predominance of enterotoxigenic Escherichia coli among ESBL/plasmid-mediated AmpC-producing strains isolated from diarrheic foals: a public health concern. Acta Vet Scand, 66(1), 54. https://doi.org/10.1186/s13028-024-00774-6

Publication

Acta veterinaria Scandinavica
ISSN: 1751-0147
NlmUniqueID: 0370400
Country: England
Language: English
Volume: 66
Issue: 1
Pages: 54
PII: 54

Researcher Affiliations

Samir, Ahmed
  • Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, Cairo, Egypt.
Abdel-Moein, Khaled A
    Zaher, Hala M
    • Department of Zoonoses, Faculty of Veterinary Medicine, Cairo University, Cairo, Egypt. drhalazaher@cu.edu.eg.

    MeSH Terms

    • Animals
    • Horses
    • Diarrhea / veterinary
    • Diarrhea / microbiology
    • Escherichia coli Infections / veterinary
    • Escherichia coli Infections / microbiology
    • Escherichia coli Infections / epidemiology
    • beta-Lactamases / genetics
    • beta-Lactamases / metabolism
    • Horse Diseases / microbiology
    • Enterotoxigenic Escherichia coli / genetics
    • Enterotoxigenic Escherichia coli / drug effects
    • Enterotoxigenic Escherichia coli / isolation & purification
    • Bacterial Proteins / genetics
    • Bacterial Proteins / metabolism
    • Plasmids / genetics
    • Public Health

    Conflict of Interest Statement

    The authors declare that they have no competing interests.

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