Proliferation of equine bone marrow-derived mesenchymal stem cells in gelatin/β-tricalcium phosphate sponges.
Abstract: A three dimensional scaffold is essential in mesenchymal stem cells (MSCs) delivery in cell-based therapy for facilitating cell adherence, migration, proliferation, and differentiation. The objectives of this study were to evaluate the possibility of β-tricalcium phosphate incorporated gelatin sponges (Gelatin/β-TCP sponge) as scaffolds for equine MSCs and to examine the effects of seeding density and seeding method on the proliferation of equine MSCs in the Gelatin/β-TCP sponges. Mononuclear cells and MSCs isolated from bone marrow were seeded into Gelatin/β-TCP sponges at different densities by different seeding methods-static or agitated methods. Proliferation of the MSCs in Gelatin/β-TCP was assessed using the Cell Counting Kit-8 assay and histological examination. Distribution and proliferation of MSCs in the Gelatin/β-TCP sponge were observed, and the Gelatin/β-TCP sponge supported limited growth when seeded at high density. We also found that the agitated seeding method enhanced the proliferation of MSCs. This study demonstrated the suitability of Gelatin/β-TCP sponges for the proliferation and maintenance of equine MSCs. These results contribute to the application of MSC-seeded Gelatin/β-TCP sponges in equine medicine.
Copyright © 2012 Elsevier Ltd. All rights reserved.
Publication Date: 2012-03-15 PubMed ID: 22424884DOI: 10.1016/j.rvsc.2012.02.013Google Scholar: Lookup
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Summary
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This research investigates the use of gelatin/β-tricalcium phosphate sponges as scaffolds for the growth and maintenance of horse bone marrow-derived mesenchymal stem cells. The study also examines how the seeding density and method influence the cells’ proliferation.
Objectives and Methods
- The purpose of the study was two-fold: firstly, to determine if β-tricalcium phosphate incorporated gelatin sponges could function as scaffolds for equine mesenchymal stem cells, and secondly, to inspect the influence of seeding density and method on the proliferation of these cells in the sponges.
- The researchers used mononuclear cells and mesenchymal stem cells drawn from bone marrow.
- These cells were then inserted into the gelatin/β-tricalcium phosphate sponges, with densities and methods of insertion varying between static and agitated conditions.
- The proliferation of cells was evaluated through the use of a Cell Counting Kit-8 assay as well as histological examination.
Results
- The study found that it was indeed possible to observe the distribution and proliferation of mesenchymal stem cells in the gelatin/β-tricalcium phosphate sponge.
- The sponge was able to support limited growth when the cells were seeded at a high density.
- The method of seeding was also found to influence proliferation – an agitated insertion method was found to enhance the proliferation of the mesenchymal stem cells.
Conclusion and Implications
- The research concluded that gelatin/β-tricalcium phosphate sponges are suitable materials for the proliferation and maintenance of equine mesenchymal stem cells.
- This conclusion is significant as it can have practical applications in equine medicine, particularly in cell-based therapies that require effective scaffolds for stem cell growth.
Cite This Article
APA
Seo JP, Tsuzuki N, Haneda S, Yamada K, Furuoka H, Tabata Y, Sasaki N.
(2012).
Proliferation of equine bone marrow-derived mesenchymal stem cells in gelatin/β-tricalcium phosphate sponges.
Res Vet Sci, 93(3), 1481-1486.
https://doi.org/10.1016/j.rvsc.2012.02.013 Publication
Researcher Affiliations
- Department of Clinical Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro-city, Hokkaido 080-8555, Japan.
MeSH Terms
- Animals
- Bone Marrow Cells / cytology
- Calcium Phosphates / chemistry
- Cell Proliferation
- Female
- Gelatin / chemistry
- Horses
- Male
- Mesenchymal Stem Cells / cytology
- Mesenchymal Stem Cells / physiology
- Tissue Engineering / methods
- Tissue Engineering / veterinary
- Tissue Scaffolds / veterinary
Citations
This article has been cited 4 times.- Seo JP, Yamaga T, Tsuzuki N, Yamada K, Haneda S, Furuoka H, Tabata Y, Sasaki N. Minimally invasive proximal interphalangeal joint arthrodesis using a locking compression plate and tissue engineering in horses: a pilot study.. Can Vet J 2014 Nov;55(11):1050-6.
- Seo JP, Tsuzuki N, Haneda S, Yamada K, Furuoka H, Tabata Y, Sasaki N. Osteoinductivity of gelatin/β-tricalcium phosphate sponges loaded with different concentrations of mesenchymal stem cells and bone morphogenetic protein-2 in an equine bone defect model.. Vet Res Commun 2014 Mar;38(1):73-80.
- Tsuzuki N, Seo JP, Yamada K, Haneda S, Furuoka H, Tabata Y, Sasaki N. The effect of a gelatin β-tricalcium phosphate sponge loaded with mesenchymal stem cells (MSC), bone morphogenic protein-2, and platelet-rich plasma (PRP) on equine articular cartilage defect.. Can Vet J 2013 Jun;54(6):573-80.
- Kanitkar M, Jaiswal A, Deshpande R, Bellare J, Kale VP. Enhanced growth of endothelial precursor cells on PCG-matrix facilitates accelerated, fibrosis-free, wound healing: a diabetic mouse model.. PLoS One 2013;8(7):e69960.
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