Prophylactic Efficacy of Equine Immunoglobulin F(ab’)2 Fragments Against Feline Parvovirus.
Abstract: Feline parvovirus (FPV), a type of parvovirus prevalent worldwide, can cause foetal death and acute enteritis in adult cats with severe leukopenia, and yet there are no effective drugs to prevent or treat FPV. Here, the immune effects of two FPV vaccines on horses were compared. IgG was extracted from FPV-immunized horse sera. Equine F(ab') fragments were obtained from pepsin-digested IgG and then purified by protein-G column chromatography. The results showed that the inactivated FPV oil vaccine was more effective than the inactivated FPV propolis vaccine in helping healthy horses to produce hyper-immune serum. Four methods were tested, among which the optimized octanoic acid-ammonium sulphate precipitation method was proved to be the best process for extracting IgG. The optimal condition for preparing F(ab') by pepsin digestion was 30 °C for 3.5 h, and the content, purity and recovery of F(ab') were 8.64 mg/mL, 90.36% and 93.24%, respectively. Our equine immunoglobulin F(ab') fragments effectively neutralized activity in vitro against FPV, alleviated the clinical symptoms of FPV-infected cats, reduced the viral loads in the intestine and had prophylactic effects in FPV-infected cats. These results indicate that the F(ab') fragment prepared from inactivated FPV-immunized horses may be used as a prophylactic agent for diseases caused by FPV.
© 2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
Publication Date: 2021-06-04 PubMed ID: 34086256PubMed Central: PMC8175436DOI: 10.1007/s12010-021-03591-zGoogle Scholar: Lookup
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Summary
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The research article focuses on examining the preventive and therapeutic potential of Equine Immunoglobulin F(ab’)2 fragments against Feline Parvovirus, a globally prevalent virus causing fatalities and severe illnesses in cats.
Study Objective and Methodology
- The study was pointed towards exploring the prophylactic (preventive) efficiency of Equine Immunoglobulin F(ab’)2 fragments against Feline Parvovirus (FPV).
- Two kinds of FPV vaccines were compared for their immune effects on horses: the inactivated FPV oil vaccine and the inactivated FPV propolis vaccine.
- The method involved extracting IgG from the sera of horses immunized with FPV, digesting IgG with pepsin to obtain F(ab’) fragments, and then purifying these using protein-G column chromatography.
Key Findings
- It was observed that the inactivated FPV oil vaccine was superior to the inactivated FPV propolis vaccine in enabling healthy horses to create hyper-immune serum.
- Among four different methods, the octanoic acid-ammonium sulfate precipitation method was identified as the best process for extracting the IgG.
- The ideal condition for preparing F(ab’) through pepsin digestion was determined to be at a temperature of 30 °C for a duration of 3.5 hours, and the content, purity and recovery rate of F(ab’) were found to be 8.64 mg/mL, 90.36% and 93.24%, respectively.
Conclusions and Implications
- Research indicated that equine immunoglobulin F(ab’) fragments effectively neutralized the in vitro activity against FPV. It relieved the clinical symptoms in FPV-infected cats, lowered the viral loads in the intestine, and showed prophylactic effects.
- These results suggest the possible use of F(ab’) fragments prepared from inactivated FPV-immunized horses as a preventive agent for diseases triggered by FPV. This novel approach could lead to the development of a functional prevention strategy for FPV and similar diseases in cats.
Cite This Article
APA
Liu J, Zhang Z, Bai A, Sha Y, Ma L, Qin S, Chen F, Qin S, Wu J.
(2021).
Prophylactic Efficacy of Equine Immunoglobulin F(ab’)2 Fragments Against Feline Parvovirus.
Appl Biochem Biotechnol, 193(10), 3151-3162.
https://doi.org/10.1007/s12010-021-03591-z Publication
Researcher Affiliations
- Guangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning, 530001, China.
- Guangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning, 530001, China.
- Guangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning, 530001, China.
- Guangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning, 530001, China.
- Guangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning, 530001, China.
- Guangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning, 530001, China.
- Guangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning, 530001, China.
- Guangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning, 530001, China.
- Guangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning, 530001, China. wu-jm20@163.com.
MeSH Terms
- Animals
- Feline Panleukopenia Virus
- Horses
- Immunoglobulin Fab Fragments
Grant Funding
- 2016YFD0501002 / Project Topics of National Key R&D Plans
- GuiKe Zhuanxiang17-4 / Fundamental Research Funds for Guangxi Veterinary Research Institute
Conflict of Interest Statement
The authors declare no competing interests.
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Citations
This article has been cited 1 times.- Liu M, Ma B, Wang Y, Chen E, Li J, Zhang M. Research on Rapid Detection Technology for β(2)-Agonists: Multi-Residue Fluorescence Immunochromatography Based on Dimeric Artificial Antigen.. Foods 2022 Mar 18;11(6).
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