Protective Effect of Silibinin on Lipopolysaccharide-Induced Inflammatory Responses in Equine Peripheral Blood Mononuclear Cells, an In Vitro Study.
Abstract: Although inflammation is an important physiological response, it plays a prominent role in several diseases across the mammalian species. In horses, in particular, inflammation secondary to bacterial infection or translocation is one of the most frequent causes of morbidity and mortality. Research in new molecules with anti-inflammatory and immunomodulatory proprieties and safe use profile is constantly an active field; natural compounds are an important source of molecules with peculiar properties such as antioxidants, anti-inflammatory and immune modulating. Silibinin, a natural polyphenolic flavonoid, extracted from plant milk thistle, has been reported to have actions such as antioxidant immunomodulatory and anti-inflammatory. The aim of this study was to test the effect of silibinin on lipopolysaccharide (LPS)-induced inflammatory response in equine peripheral blood mononuclear cells (PBMCs). Our results showed the protective effect of silibinin 10 μM and 50 μM in equine PBMCs stimulated with LPS. Silibilinin was able to prevent the LPS induced increased levels of TNF-α, IL-1β, IL-6 and IL-8. The results from this study on LPS-stimulated equine PBMCs showed that silibinin could be a useful pharmacological approach in treatment or prevention of several inflammatory conditions in horse.
Publication Date: 2020-11-03 PubMed ID: 33153060PubMed Central: PMC7692186DOI: 10.3390/ani10112022Google Scholar: Lookup
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- Journal Article
Summary
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This research attempts to determine the effects of silibinin, a compound derived from milk thistle, on inflammation in horses. The study found that silibinin was able to stave off inflammation, particularly from bacterial infections, suggesting this compound could be beneficial in preventing or treating several inflammatory conditions in horses.
Background
- Inflammation, though a necessary physiological response, is a principal cause of numerous diseases among mammals and is often the result of bacterial infections. It is a particularly prevalent cause of morbidity and mortality in horses. As such, there is ongoing research into new molecules with anti-inflammatory and immune-modulating properties that are also safe for use.
- Natural compounds are a significant area of investigation for such molecules. Silibinin, a polyphenolic flavonoid extracted from milk thistle, is one such compound. Previous studies have shown its potential as an antioxidant, immunomodulator, and anti-inflammatory agent, making it a candidate for medical uses.
Method and Results
- This study’s aim was to test silibinin’s impact on inflammation induced by Lipopolysaccharides (LPS) in equine peripheral blood mononuclear cells (PBMCs). Animals’ PBMCs frequently serve as models in immunological studies, and LPS, often found in bacterial cell walls, are known to trigger strong immune responses.
- Results indicated that silibinin had a protective effect on equine PBMCs stimulated with LPS. Specifically, treatment with 10 µM and 50 µM of silibinin was able to prevent increased levels of pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and IL-8 which are typically induced by LPS.
Conclusion
- The findings from this study suggest that silibinin could contribute to the prevention or treatment of various inflammatory conditions in horses by modulating the inflammatory response. By mitigating the LPS-induced inflammatory response, silibinin could potentially decrease the severity or incidence of diseases associated with inflammatory complications in horses.
- However, more comprehensive studies would be necessary in order to fully understand the safety, efficacy, and application of silibinin in real-world scenarios.
Cite This Article
APA
Gugliandolo E, Crupi R, Biondi V, Licata P, Cuzzocrea S, Passantino A.
(2020).
Protective Effect of Silibinin on Lipopolysaccharide-Induced Inflammatory Responses in Equine Peripheral Blood Mononuclear Cells, an In Vitro Study.
Animals (Basel), 10(11), 2022.
https://doi.org/10.3390/ani10112022 Publication
Researcher Affiliations
- Department of Chemical, Biological, Pharmaceutical and Environmental Science, University of Messina, 98166 Messina, Italy.
- Department of Veterinary Science, University of Messina, 98168 Messina, Italy.
- Department of Veterinary Science, University of Messina, 98168 Messina, Italy.
- Department of Veterinary Science, University of Messina, 98168 Messina, Italy.
- Department of Chemical, Biological, Pharmaceutical and Environmental Science, University of Messina, 98166 Messina, Italy.
- Department of Pharmacological and Physiological Science, Saint Louis University, School of Medicine, 1402 South Grand Blvd, St Louis, MO 63104, USA.
- Department of Veterinary Science, University of Messina, 98168 Messina, Italy.
Conflict of Interest Statement
The authors declare no conflict of interest.
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