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Home / Equine Research Bank / Anim Sci J / Quantitative analysis of the serum proteome during early pre...
Animal science journal = Nihon chikusan Gakkaiho2022; 93(1); e13727; doi: 10.1111/asj.13727

Quantitative analysis of the serum proteome during early pregnancy in mares.

Abstract: Equine pregnancy is currently diagnosed by rectal palpation, ultrasonographic examination, or by measuring changes in hormones in the blood. In the present study, we identified proteins that are differentially expressed in the sera of early pregnant and non-pregnant mares in order to develop a novel method for diagnosing equine pregnancy. Serum samples were obtained from 18 adult mares, pregnancy at day 32 after ovulation (n = 9) and in diestrus (n = 9). Proteomic analysis of the samples was conducted using liquid chromatography-electrospray ionization-tandem mass spectrometry. We identified 467 proteins from a total of 3514 peptides. Thirty-two proteins (15 upregulated and 17 downregulated) were significantly differentially expressed between the two groups. The Gene Ontology enrichment analysis revealed that they are related to extracellular matrix assembly, blood coagulation, and hemostasis, and the prominent molecular functions were integrin binding, cell adhesion molecule binding, and glycine C-acetyltransferase activity. The pathway analysis of Kyoto Encyclopaedia of Genes and Genomes showed that the top three pathways identified were glycine, serine, and threonine metabolism; cysteine and methionine metabolism; and ether lipid metabolism. The selected five serum proteins were newly potential candidates for pregnancy diagnosis in mares.
© 2022 Japanese Society of Animal Science.
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Publication Date: 2022-04-28 PubMed ID: 35476278DOI: 10.1111/asj.13727Google Scholar: Lookup
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Summary

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The research article discusses the identification of proteins that show altered expression in the blood serum of pregnant horses, with the hope of developing new procedures for detecting pregnancy in mares.

Introduction

  • The researchers set out to improve current methods for diagnosing pregnancy in mares (female horses) by identifying proteins in the blood serum that are expressed differently in early pregnancy.
  • Presently, pregnancy in equines is determined through techniques like rectal palpation, ultrasound examinations, or tracking hormone fluctuations in the blood. This study aims to offer a novel, potentially less invasive and more efficient method.

Methodology

  • Serum samples were collected from 18 mature mares. Nine of them were pregnant (at day 32 after ovulation), and the other nine were in the diestrus phase of their reproductive cycle.
  • The team used a proteomic technique known as liquid chromatography-electrospray ionization-tandem mass spectrometry to evaluate the samples.

Results

  • Analysis of the samples resulted in the identification of 467 proteins from a total of 3514 peptides.
  • Out of these, 32 proteins showed significant differential expression between the pregnant and non-pregnant mares, with 15 proteins being upregulated (increased expression) and 17 being downregulated (decreased expression) during early pregnancy.

Further Analysis and Interpretation

  • Through Gene Ontology enrichment analysis, it was discovered that these differentially expressed proteins are involved in various biological processes like extracellular matrix assembly, blood coagulation, and hemostasis.
  • The proteins also showcased important molecular functions such as integrin binding, cell adhesion molecule binding, and glycine C-acetyltransferase activity.
  • The pathway analysis, performed using the Kyoto Encyclopaedia of Genes and Genomes (KEGG), indicated the highest impacted pathways to be glycine, serine, and threonine metabolism; cysteine and methionine metabolism; and ether lipid metabolism.
  • The researchers identified five serum proteins as potential candidates for new markers that could be used for diagnosing pregnancy in mares.

Conclusion

  • This study has managed to identify differentially expressed proteins in the blood serum of early pregnant mares, paving the way for an improved method of diagnosing equine pregnancy.

Cite This Article

APA
Deng L, Li Z, Tang C, Han Y, Zhang L, Liao Q. (2022). Quantitative analysis of the serum proteome during early pregnancy in mares. Anim Sci J, 93(1), e13727. https://doi.org/10.1111/asj.13727

Publication

Animal science journal = Nihon chikusan Gakkaiho
ISSN: 1740-0929
NlmUniqueID: 100956805
Country: Australia
Language: English
Volume: 93
Issue: 1
Pages: e13727

Researcher Affiliations

Deng, Liang
  • Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, China.
Li, Zheng
  • Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, China.
Tang, Chi
  • Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, China.
  • Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin, College of Life Sciences, Tarim University, Alar, China.
Han, Yuwei
  • Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, China.
Zhang, Linxi
  • Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, China.
Liao, Qingchao
  • Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, China.

MeSH Terms

  • Animals
  • Chromatography, Liquid / veterinary
  • Female
  • Glycine
  • Horses
  • Mass Spectrometry / veterinary
  • Pregnancy
  • Proteome
  • Proteomics / methods

Grant Funding

  • 21-116-3-39 / Foundation of Shenyang Science and Technology Transformation Project
  • 31501952 / National Natural Science Foundation of China

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