Rapid diagnosis of equine influenza by highly sensitive silver amplification immunochromatography system.
Abstract: Equine influenza (EI) is a respiratory disease caused by equine influenza A virus (EIV, H3N8) infection. Rapid diagnosis is essential to limit the disease spread. We previously reported that some rapid antigen detection (RAD) tests are fit for diagnosing EI although their sensitivity is not optimal. Here, we evaluated the performance of the newly developed RAD test using silver amplification immunochromatography (Quick Chaser Auto Flu A, B: QCA) to diagnose EI. The detection limits of QCA for EIVs were five-fold lower than the conventional RAD tests. The duration of virus antigen detection in the infected horses was longer than the conventional RAD tests. We conclude that QCA could be a valuable diagnostic method for EI.
Publication Date: 2017-05-01 PubMed ID: 28458275PubMed Central: PMC5487783DOI: 10.1292/jvms.17-0105Google Scholar: Lookup
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- Evaluation Study
- Journal Article
Summary
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This research is about a new technique for quickly diagnosing equine influenza that uses a method known as silver amplification immunochromatography, which has shown superior sensitivity to conventional rapid antigen detection tests.
Context and Importance of Research
- The research is centred around rapid diagnosis of Equine influenza (EI), a respiratory disease that occurs in horses due to infection from the equine influenza A virus (EIV, H3N8).
- Fast and accurate diagnosis is crucial to restrict the spread of this contagious disease among horses. Previously, rapid antigen detection (RAD) tests were used which, while fairly accurate, lack optimal sensitivity.
- So, the team developed a new RAD test using silver amplification immunochromatography, called Quick Chaser Auto Flu A, B: QCA, specifically aimed at increasing the sensitivity and enhancing the efficiency of EI detection.
Methodology and Results
- The researchers compared the performance of this latest RAD test to conventional RAD tests in terms of their detection limits for EIVs.
- The results indicated that the QCA could detect EIVs at levels five-fold lower than that of conventional RAD tests, showcasing its superior sensitivity.
- Also, QCA’s detection duration for viral antigens in horses post-infection was notably longer than traditional RAD tests.
Conclusions
- Based on the results, the research team concluded that the newly-developed QCA could provide significant value in the rapid diagnosis of equine influenza.
- Its better sensitivity and longer detection duration can help in identifying even low levels of virus in infected horses, thus increasing the likelihood of early diagnosis and intervention.
- This improved diagnostic capability can aid in limiting the spread of EI, thereby protecting the health and well-being of horses worldwide.
Cite This Article
APA
Yamanaka T, Nemoto M, Bannai H, Tsujimura K, Kondo T, Matsumura T, Fu TQH, Fernandez CJ, Gildea S, Cullinane A.
(2017).
Rapid diagnosis of equine influenza by highly sensitive silver amplification immunochromatography system.
J Vet Med Sci, 79(6), 1061-1063.
https://doi.org/10.1292/jvms.17-0105 Publication
Researcher Affiliations
- Equine Research Institute, the Japan Racing Association, 1400-4 Shiba, Shimotsuke, Tochigi 329-0412, Japan.
- Equine Research Institute, the Japan Racing Association, 1400-4 Shiba, Shimotsuke, Tochigi 329-0412, Japan.
- Equine Research Institute, the Japan Racing Association, 1400-4 Shiba, Shimotsuke, Tochigi 329-0412, Japan.
- Equine Research Institute, the Japan Racing Association, 1400-4 Shiba, Shimotsuke, Tochigi 329-0412, Japan.
- Equine Research Institute, the Japan Racing Association, 1400-4 Shiba, Shimotsuke, Tochigi 329-0412, Japan.
- Equine Research Institute, the Japan Racing Association, 1400-4 Shiba, Shimotsuke, Tochigi 329-0412, Japan.
- Agri-Food & Veterinary Authority of Singapore, Animal and Plant Health Centre, 6 Perahu Road, 718827 Singapore.
- Agri-Food & Veterinary Authority of Singapore, Animal and Plant Health Centre, 6 Perahu Road, 718827 Singapore.
- Irish Equine Centre, Johnstown, Naas, Co. Kildare, W91 RH93 Ireland.
- Irish Equine Centre, Johnstown, Naas, Co. Kildare, W91 RH93 Ireland.
MeSH Terms
- Animals
- Chromatography, Affinity / methods
- Chromatography, Affinity / veterinary
- Horse Diseases / diagnosis
- Horse Diseases / virology
- Horses
- Influenza A Virus, H3N8 Subtype
- Orthomyxoviridae Infections / diagnosis
- Orthomyxoviridae Infections / veterinary
- Silver
- Time Factors
References
This article includes 8 references
- Cullinane A, Newton JR. Equine influenza--a global perspective.. Vet Microbiol 2013 Nov 29;167(1-2):205-14.
- Mitamura K, Shimizu H, Yamazaki M, Ichikawa M, Nagai K, Katada J, Wada A, Kawakami C, Sugaya N. Clinical evaluation of highly sensitive silver amplification immunochromatography systems for rapid diagnosis of influenza.. J Virol Methods 2013 Dec;194(1-2):123-8.
- Wada A, Sakoda Y, Oyamada T, Kida H. Development of a highly sensitive immunochromatographic detection kit for H5 influenza virus hemagglutinin using silver amplification.. J Virol Methods 2011 Dec;178(1-2):82-6.
- Webster RG, Bean WJ, Gorman OT, Chambers TM, Kawaoka Y. Evolution and ecology of influenza A viruses.. Microbiol Rev 1992 Mar;56(1):152-79.
- Wilson WD. Equine influenza.. Vet Clin North Am Equine Pract 1993 Aug;9(2):257-82.
- Yamanaka T, Niwa H, Tsujimura K, Kondo T, Matsumura T. Epidemic of equine influenza among vaccinated racehorses in Japan in 2007.. J Vet Med Sci 2008 Jun;70(6):623-5.
- Yamanaka T, Cullinane A, Gildea S, Bannai H, Nemoto M, Tsujimura K, Kondo T, Matsumura T. The potential impact of a single amino-acid substitution on the efficacy of equine influenza vaccines.. Equine Vet J 2015 Jul;47(4):456-62.
- Yamanaka T, Nemoto M, Bannai H, Tsujimura K, Kondo T, Matsumura T, Gildea S, Cullinane A. Evaluation of twenty-two rapid antigen detection tests in the diagnosis of Equine Influenza caused by viruses of H3N8 subtype.. Influenza Other Respir Viruses 2016 Mar;10(2):127-33.
Citations
This article has been cited 2 times.- Jin X, Zhang H, Ni B, Liu W, Hou L, Marsh JH, Ye S, Sun X, Li X, Li S, Dong L, Hou JJ, Sun M, Xu B, Xiong J, Liu X. Label-free sensing of virus-like particles below the sub-diffraction limit by wide-field photon state parametric imaging of a gold nanodot array. Nanoscale Adv 2021 Dec 7;3(24):6882-6887.
- Singh RK, Dhama K, Karthik K, Khandia R, Munjal A, Khurana SK, Chakraborty S, Malik YS, Virmani N, Singh R, Tripathi BN, Munir M, van der Kolk JH. A Comprehensive Review on Equine Influenza Virus: Etiology, Epidemiology, Pathobiology, Advances in Developing Diagnostics, Vaccines, and Control Strategies. Front Microbiol 2018;9:1941.
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