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The Veterinary clinics of North America. Equine practice1995; 11(3); 437-442; doi: 10.1016/s0749-0739(17)30309-7

Recent advances in the laboratory diagnosis of equine parasitic diseases.

Abstract: This article reviews recent advances in laboratory diagnosis of equine parasitic diseases. Laboratory diagnosis of most equine parasitic diseases continues to rely on standard methods. Only laboratory diagnostic tests for EPM, cryptosporidiosis, and giardiasis were included. The criteria for testing and interpretation of results for each new diagnostic method were explained. Western blot and PCR testing for EPM and immunofluorescent staining with monoclonal antibodies for cryptosporidiosis and giardiasis were reviewed.
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Publication Date: 1995-12-01 PubMed ID: 8925419PubMed Central: PMC7134721DOI: 10.1016/s0749-0739(17)30309-7Google Scholar: Lookup
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Summary

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This research sheds light on the latest advancements in the laboratory diagnosis of equine parasitic diseases, emphasizing that the majority of the diagnoses still rely on standard methods. The study delves deeper into diagnostic tests for Equine protozoal myeloencephalitis (EPM), cryptosporidiosis, and giardiasis along with the evaluation and understanding of test results for new diagnostic methods.

Standard Diagnostic Methods:

  • The research reaffirms that most equine parasitic diseases are still diagnosed using standard laboratory methods. The essence of these techniques is to detect the presence of parasites or their eggs in the host’s faecal matter, providing a preliminary but critical indication of an infection.

Focusing on Specific Diseases:

  • The study then specifically, highlights three diseases, EPM, cryptosporidiosis, and giardiasis, for their reliance on advanced laboratory diagnostic tests.

Testing Methods:

  • Details of each new diagnostic method and the criteria for its use in testing are provided. For example, Western blot and polymerase chain reaction (PCR) testing are deemed especially useful for EPM. These advanced techniques help detect antibodies against the causative agent, Sarcocystis neurona, hence aiding in confirming EPM infection.
  • Cryptosporidiosis and giardiasis are detected through immunofluorescence staining collaborated with monoclonal antibodies. This detailed method allows for the identification of these protozoans in the sample, aiding the diagnosis of these parasitic diseases.

Interpretation of Results:

  • The research emphasizes the importance of correctly interpreting the test results. For diagnosis accuracy, understanding the limitations and benefits of each testing method is paramount. Consequently, the study provides necessary insight into the interpretation of result from each of these new diagnostic methods.

By exploring these advancements, the research aims to improve our understanding and effectiveness in diagnosing equine parasitic diseases, contributing significantly towards their treatment and control.

Cite This Article

APA
Granstrom DE. (1995). Recent advances in the laboratory diagnosis of equine parasitic diseases. Vet Clin North Am Equine Pract, 11(3), 437-442. https://doi.org/10.1016/s0749-0739(17)30309-7

Publication

The Veterinary clinics of North America. Equine practice
ISSN: 0749-0739
NlmUniqueID: 8511904
Country: United States
Language: English
Volume: 11
Issue: 3
Pages: 437-442

Researcher Affiliations

Granstrom, D E
  • Department of Veterinary Science, University of Kentucky, Lexington, USA.

MeSH Terms

  • Animals
  • Blotting, Western / veterinary
  • Cryptosporidiosis / diagnosis
  • Cryptosporidiosis / veterinary
  • Encephalomyelitis / diagnosis
  • Encephalomyelitis / veterinary
  • Fluorescent Antibody Technique, Direct / veterinary
  • Giardiasis / diagnosis
  • Giardiasis / veterinary
  • Horse Diseases / diagnosis
  • Horses
  • Parasitic Diseases / diagnosis
  • Parasitic Diseases, Animal
  • Protozoan Infections / diagnosis
  • Protozoan Infections, Animal

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This article includes 22 references
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Citations

This article has been cited 1 times.
  1. Liang FT, Granstrom DE, Zhao XM, Timoney JF. Evidence that surface proteins Sn14 and Sn16 of Sarcocystis neurona merozoites are involved in infection and immunity. Infect Immun 1998 May;66(5):1834-8.
    doi: 10.1128/IAI.66.5.1834-1838.1998pubmed: 9573058google scholar: lookup
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