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Journal of applied microbiology2016; 122(1); 23-29; doi: 10.1111/jam.13298

Reducing exposure to pathogens in the horse: a preliminary study into the survival of bacteria on a range of equine bedding types.

Abstract: To compare the rate of growth of four microbial strains that cause disease in the horse, on four commonly used types of bedding. The moisture-holding capacity of each bedding type was also tested. Results: Microbial strains included Streptococcus equi, Streptococcus zooepidemicus, Fusobacterium necrophorum, Dichelobacter nodosus and Dermatophilus congolensis. The bedding types tested were Pinus sylvestris (Scots pine shavings), Pinus nigra (Corsican pine shavings), Picea sitchensis (Sitka spruce shavings), Cannabis sativa (hemp) and chopped wheat straw. A suspension of each microbial strain was spread in triplicate on agar media and incubated in its optimal growth conditions. The viable count (colony-forming unit per ml) was determined for each bacterial strain for the five different bedding types. Pinus sylvestris bedding resulted in significantly less (P = 0·001) bacterial growth of all strains tested. Conclusions: Factors resulting in the inhibition of bacterial growth include the antibacterial effects reported in the Pinacea family and the physical properties of the bedding substrate. Research is currently focussed on the diagnosis and management of disease. Prevention of disease is also important for matters of biosecurity. Strategies should include the provision of a hygienic environment and the use of specific types of bedding. Conclusions: Bedding choice has implications for global equine health and disease prevention as well as potential benefits in other animal species.
Publication Date: 2016-11-23 PubMed ID: 27659898DOI: 10.1111/jam.13298Google Scholar: Lookup
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  • Journal Article

Summary

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This research study explores the growth of disease-causing bacteria on different types of horse bedding, and concludes that Pinus sylvestris bedding shows significantly less bacterial growth, indicating its potential for disease prevention in equine health.

Study Objective and Methodology

  • The study aimed to investigate the rate of growth of four strains of microbes that infest horses on different types of commonly used bedding.
  • In addition, the capacity of each bedding type to hold moisture was examined.
  • The bacterial strains tested were Streptococcus equi, Streptococcus zooepidemicus, Fusobacterium necrophorum, Dichelobacter nodosus and Dermatophilus congolensis.
  • The bedding types used were Pinus sylvestris (Scots pine shavings), Pinus nigra (Corsican pine shavings), Picea sitchensis (Sitka spruce shavings), Cannabis sativa (hemp) and chopped wheat straw.
  • Each bacterial strain was included in a suspension and spread in triplicate on agar media and then incubated in optimal growth conditions.

Findings

  • The viable count (colony-forming unit per ml) of each bacterial strain was then determined for the five different bedding types.
  • The most significant finding showed that the bedding made from Pinus sylvestris resulted in considerably less bacterial growth of all tested strains.

Conclusion and Implications

  • This decreased bacterial growth could be due to antibacterial properties associated with the Pinacea family and the physical properties of the bedding material.
  • The study’s findings provide practical insight for disease prevention in the context of equine health and biosecurity, suggesting that choosing a hygienic environment includes using specific types of bedding.
  • The choice of appropriate bedding has wider implications for global equine health and disease prevention, potentially offering benefits for other animal species as well.

Cite This Article

APA
Yarnell K, Le Bon M, Turton N, Savova M, McGlennon A, Forsythe S. (2016). Reducing exposure to pathogens in the horse: a preliminary study into the survival of bacteria on a range of equine bedding types. J Appl Microbiol, 122(1), 23-29. https://doi.org/10.1111/jam.13298

Publication

ISSN: 1365-2672
NlmUniqueID: 9706280
Country: England
Language: English
Volume: 122
Issue: 1
Pages: 23-29

Researcher Affiliations

Yarnell, K
  • School of Animal, Rural and Environmental Sciences, Nottingham Trent University, Southwell, Nottingham, UK.
Le Bon, M
  • School of Animal, Rural and Environmental Sciences, Nottingham Trent University, Southwell, Nottingham, UK.
Turton, N
  • School of Science and Technology, Nottingham Trent University, Nottingham, UK.
Savova, M
  • School of Science and Technology, Nottingham Trent University, Nottingham, UK.
McGlennon, A
  • School of Animal, Rural and Environmental Sciences, Nottingham Trent University, Southwell, Nottingham, UK.
Forsythe, S
  • School of Science and Technology, Nottingham Trent University, Nottingham, UK.

MeSH Terms

  • Actinobacteria / classification
  • Actinobacteria / growth & development
  • Actinobacteria / physiology
  • Animals
  • Bacterial Infections / microbiology
  • Bacterial Infections / prevention & control
  • Bacterial Infections / transmission
  • Bacterial Infections / veterinary
  • Cannabis
  • Environmental Microbiology
  • Fusobacterium / growth & development
  • Fusobacterium / physiology
  • Horse Diseases / microbiology
  • Horse Diseases / prevention & control
  • Horse Diseases / transmission
  • Horses
  • Housing, Animal
  • Hygiene
  • Pinus
  • Streptococcus / classification
  • Streptococcus / growth & development
  • Streptococcus / physiology
  • Streptococcus equi / growth & development
  • Streptococcus equi / physiology
  • Triticum

Citations

This article has been cited 4 times.
  1. Holmes TQ, Brown AF. Champing at the Bit for Improvements: A Review of Equine Welfare in Equestrian Sports in the United Kingdom. Animals (Basel) 2022 May 5;12(9).
    doi: 10.3390/ani12091186pubmed: 35565612google scholar: lookup
  2. Butler D, Valenchon M, Annan R, Whay HR, Mullan S. Stakeholder Perceptions of the Challenges to Racehorse Welfare. Animals (Basel) 2019 Jun 17;9(6).
    doi: 10.3390/ani9060363pubmed: 31212903google scholar: lookup
  3. Masebo NT, Benedetti B, Mountricha M, Lee L, Padalino B. A Literature Review on Equine Bedding: Impacts on Horse and Human Welfare, Health, and the Environment. Animals (Basel) 2025 Mar 5;15(5).
    doi: 10.3390/ani15050751pubmed: 40076033google scholar: lookup
  4. Mańkowska A, Witkowska D. The Most Common Environmental Risk Factors for Equine Asthma-A Narrative Review. Animals (Basel) 2024 Jul 14;14(14).
    doi: 10.3390/ani14142062pubmed: 39061524google scholar: lookup