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International orthopaedics2011; 35(10); 1569-1576; doi: 10.1007/s00264-011-1237-3

Regenerative medicine for the treatment of musculoskeletal overuse injuries in competition horses.

Abstract: Tissue repair in musculoskeletal injuries is often a slow and sometimes incomplete process. Regenerative medicine based on the use of growth factors (GFs) and cell therapy is aimed at improving the quality and speed of tendon and ligament healing. The aim of this study was to evaluate the potential for the administration of a combination of autologous platelet-rich plasma (PRP) and freshly isolated bone marrow mononucleated cells (BMMNCs) in 13 competition horses affected by overuse musculoskeletal injuries (suspensory ligament desmopathy and superficial flexor tendinopathy) and refractory to other therapies. Methods: After ultrasonographic localisation of the lesion, the autologous BMMNC suspension and PRP were injected directly into the core lesion. BMMNC and platelet count as well as growth factors in PRP were measured to determine factors influencing the clinical outcome. Results: Horses showed a marked improvement in their degree of lameness and 84.6% were able to return to competition. Among all the factors studied, the platelet concentration predicted the healing time: significantly faster recovery (p = 0.049) was observed in cases of PRP with more than 750 × 10(3)/μl platelets. Conclusions: Competition horses are involved in highly demanding activities, thus being a similar model for the high mechanical overload typical of human athletes. The promising results obtained suggest that this combined biological approach may be useful even for the treatment of recalcitrant overuse musculoskeletal injuries in highly demanding patients if the appropriate dose of cells and GFs is applied.
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Publication Date: 2011-03-11 PubMed ID: 21394594PubMed Central: PMC3174295DOI: 10.1007/s00264-011-1237-3Google Scholar: Lookup
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  • Journal Article
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article explores using regenerative medicine to hasten and improve healing of overuse injuries in competition horses. It particularly focuses on the use of platelet-rich plasma and bone marrow mononucleated cells in treating tendon and ligament injuries.

Methods

  • The study involved 13 competition horses that were experiencing musculoskeletal injuries and were unsuccessful in responding to other forms of treatment.
  • Ultrasound was used to locate the physical anomaly, before a combination of both autologous platelet-rich plasma (PRP) and freshly isolated bone marrow mononucleated cells (BMMNCs) was injected directly into the injured area.
  • The researchers measured the concentration of BMMNC and platelets, including the quantity of growth factors in the PRP, in order to assess their impact on the clinical outcome.

Results

  • The horses exhibited a significant reduction in lameness following treatment and an impressive 84.6% were even able to return to competition.
  • The study found that the platelet concentration was a major determinant of the healing time. More specifically, a significantly faster recovery was noticed when the PRP contained more than 750 × 10^3/μl platelets.

Conclusions

  • The researchers deemed the results encouraging, suggesting that this combined biological approach could also be applied in the treatment of human athletes with similar kinds of musculoskeletal overuse injuries.
  • However, the research suggests that the prospect of the treatment’s success may be reliant on the correct dosage of cells and growth factors.

Cite This Article

APA
Torricelli P, Fini M, Filardo G, Tschon M, Pischedda M, Pacorini A, Kon E, Giardino R. (2011). Regenerative medicine for the treatment of musculoskeletal overuse injuries in competition horses. Int Orthop, 35(10), 1569-1576. https://doi.org/10.1007/s00264-011-1237-3

Publication

International orthopaedics
ISSN: 1432-5195
NlmUniqueID: 7705431
Country: Germany
Language: English
Volume: 35
Issue: 10
Pages: 1569-1576

Researcher Affiliations

Torricelli, Paola
  • Laboratory of Preclinical and Surgical Studies, Research Institute Codivilla-Putti, Rizzoli Orthopaedic Institute, Via di Barbiano 1/10, 40136, Bologna, Italy.
Fini, Milena
    Filardo, Giuseppe
      Tschon, Matilde
        Pischedda, Mario
          Pacorini, Alessandra
            Kon, Elizaveta
              Giardino, Roberto

                MeSH Terms

                • Animals
                • Athletic Injuries / therapy
                • Athletic Injuries / veterinary
                • Cell- and Tissue-Based Therapy / methods
                • Cell- and Tissue-Based Therapy / veterinary
                • Cumulative Trauma Disorders / diagnostic imaging
                • Cumulative Trauma Disorders / therapy
                • Cumulative Trauma Disorders / veterinary
                • Female
                • Horse Diseases / diagnostic imaging
                • Horse Diseases / therapy
                • Horses / physiology
                • Leukocytes, Mononuclear / cytology
                • Leukocytes, Mononuclear / transplantation
                • Male
                • Platelet Transfusion / veterinary
                • Platelet-Rich Plasma / cytology
                • Regenerative Medicine / methods
                • Sports
                • Tissue Engineering / veterinary
                • Ultrasonography

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