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Equine veterinary journal2019; 52(1); 126-130; doi: 10.1111/evj.13119

Repeatability of the hyperglycaemic clamp for assessment of β-cell response and insulin sensitivity in horses.

Abstract: The hyperglycaemic clamp has been used for measurement of insulin sensitivity (IS) but not for measurement of β-cell response in the horse. However, the repeatability of this test has not been reported for horses. Objective: To determine the repeatability and reliability of measures for IS and β-cell response to glucose using the hyperglycaemic clamp in horses. Methods: Repeated measures, longitudinal study. Methods: Six healthy Standardbred mares underwent a 120-min hyperglycaemic clamp on two occasions with a 10-day washout period. Indices of repeatability and reliability were calculated from measures of IS and β-cell response to glucose derived from each hyperglycaemic clamp. Results: Measures of β-cell response to glucose, including area under the insulin response curve in the time interval 0-120 min and during steady state (60-120 min), as well as the mean insulin concentration during steady state had coefficient of variations (CV) of 9.5, 10.5 and 9.3 respectively and intraclass correlation coefficients (ICC) of 0.93, 0.93 and 0.95 respectively. The measure of glucose disposal (M) had lower CV (12.4) and ICC (0.71) compared to measure of IS (M/I-index; CV of 17.4 and ICC of 0.89). Conclusions: The study was conducted in a small number of horses. Conclusions: The hyperglycaemic clamp is a diagnostic method for assessing β-cell response to intravenous glucose with high repeatability and reliability. Insulin sensitivity can be determined with the same test but the repeatability for these measurements is lower compared to the measurements for β-cell response.
© 2019 EVJ Ltd.
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Publication Date: 2019-05-08 PubMed ID: 30980682DOI: 10.1111/evj.13119Google Scholar: Lookup
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  • Clinical Trial
  • Veterinary
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study explores the repeatability and reliability of using the hyperglycaemic clamp to measure insulin sensitivity and β-cell response to glucose in horses. The findings suggest that the test is highly consistent and reliable for assessing β-cell response, but less so for evaluating insulin sensitivity.

Methods

  • The researchers used a repeated measures, longitudinal study design, involving six healthy Standardbred mares.
  • The horses underwent a 120-minute hyperglycaemic clamp test twice, with a 10-day washout period between tests.
  • The hyperglycaemic clamp is a diagnostic test that involves maintaining a constant elevated glucose level for a specific period to evaluate how the body responds.
  • The researchers looked at various measurements such as the area under the insulin response curve during the 0-120 minute test and the mean insulin concentration during a steady state. They also calculated repeatability and reliability indices from these measurements.

Results

  • Measures of β-cell response to glucose, which indicate how well the insulin-producing cells in the pancreas respond to the increase in glucose, showed good repeatability and reliability.
  • The coefficients of variation, a measure of variability relative to the mean, for this response ranged between 9.3 and 10.5, suggesting a low level of variability.
  • The intraclass correlation coefficients, a measure of reliability or consistency of measures, ranged between 0.93 and 0.95, indicating high reliability.
  • Insulin sensitivity, which indicates how effectively the cells of the body absorb glucose in response to insulin, showed less repeatability compared to the measurements for β-cell response.

Conclusions

  • The study, however, was conducted on a small number of horses, which could limit the applicability of the findings.
  • The researchers concluded that the hyperglycaemic clamp test is a reliable method for assessing β-cell response to intravenous glucose.
  • They also noted that while the same test can be used to determine insulin sensitivity, the repeatability for such measurements is lower compared to when the test is used to measure β-cell response.

Cite This Article

APA
Lindåse S, Johansson H, Månsby M, Bröjer J. (2019). Repeatability of the hyperglycaemic clamp for assessment of β-cell response and insulin sensitivity in horses. Equine Vet J, 52(1), 126-130. https://doi.org/10.1111/evj.13119

Publication

Equine veterinary journal
ISSN: 2042-3306
NlmUniqueID: 0173320
Country: United States
Language: English
Volume: 52
Issue: 1
Pages: 126-130

Researcher Affiliations

Lindåse, S
  • Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Johansson, H
  • Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Månsby, M
  • Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Bröjer, J
  • Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden.

MeSH Terms

  • Animals
  • Blood Glucose
  • Female
  • Glucose Clamp Technique / veterinary
  • Horses / blood
  • Horses / physiology
  • Insulin Resistance / physiology
  • Insulin-Secreting Cells / drug effects
  • Insulin-Secreting Cells / metabolism

Grant Funding

  • Swedish University of Agricultural Sciences

References

This article includes 23 references
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Citations

This article has been cited 1 times.
  1. Lindåse S, Nostell K, Bergsten P, Forslund A, Bröjer J. Evaluation of fasting plasma insulin and proxy measurements to assess insulin sensitivity in horses. BMC Vet Res 2021 Feb 15;17(1):78.
    doi: 10.1186/s12917-021-02781-5pubmed: 33588833google scholar: lookup
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