FREE SHIPPING over $40
OVER 9000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Home / Equine Research Bank / Vet Immunol Immunopathol / Report of the Second Equine Leucocyte Antigen Workshop, Squa...
Veterinary immunology and immunopathology1998; 62(2); 101-143; doi: 10.1016/s0165-2427(97)00160-8

Report of the Second Equine Leucocyte Antigen Workshop, Squaw valley, California, July 1995.

Abstract: The final assignment of antibody clusters for leucocyte antigens and immunoglobulins, as described in detail in Sections 3 and 4, is summarized in Table 4. Together with other mAbs developed outside of ELAW II (Table 9) this pool of reagents represent a powerful array of tools for the study of equine immunity. The Second Equine Leucocyte Antigen Workshop made considerable advances in pursuing the objectives of establishing the specificities of mAbs and achieving consensus on the nomenclature for equine leucocyte and immunoglobulin molecules. Of equal importance, several productive collaborations were fostered among the participating laboratories and observers. Overall, enormous advances have been made in the past decade since mAbs specific for equine leucocyte antigens and immunoglobulins were first reported. There remains enormous scope and need for further studies of equine leucocyte antigens and immunoglobulins, both for the purposes of comparative immunology and for the good of the horse. In the future novel techniques will be required to develop reagents for specific target antigens such as the orthologues of the CD25 or CD45 isoforms. In studies of equine immunoglobulins the functional role of the IgG isotypes must be better established, reagents for IgE must be developed, and cloning of the immunoglobulin heavy chain genes will be essential if the complexities of the IgG sub-isotypes are to be elucidated. The tasks still facing the currently small group of equine immunologists throughout the world remain formidable, and will only be tackled successfully in a spirit of collaboration.
Get Full Text
Publication Date: 1998-06-25 PubMed ID: 9638857DOI: 10.1016/s0165-2427(97)00160-8Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Congress
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article reports on the progress made during the Second Equine Leucocyte Antigen Workshop, where scientists aimed to better understand specificities of monoclonal antibodies related to equine leucocyte and immunoglobulin molecules, establish standard nomenclature, and explore ways to further advancement in the field of equine immunology.

Objectives of the Second Equine Leucocyte Antigen Workshop

  • The workshop aimed to establish the specificities of monoclonal antibodies (mAbs) which are identical immune cells derived from a unique parent cell.
  • It aimed to create a consensus on nomenclature for equine leucocyte and immunoglobulin molecules. Leucocytes are white blood cells, while immunoglobulin molecules are antibodies.
  • The workshop also sought to foster collaborations among the participating labs and observers.

Findings of the Workshop

  • The workshop resulted in the assignment of antibody clusters for leucocyte antigens and immunoglobulins, summarized with the aid of tables.
  • These findings, in conjunction with other monoclonal antibodies (mAbs) developed outside of the workshop, were considered a robust array of tools for studying equine immunity.

Future Approaches

  • The report acknowledges a continuing need for deeper studies of equine leucocyte antigens and immunoglobulins, not only for the purposes of comparative immunology but also for the benefit of horse health.
  • Future research will need to deploy novel techniques to develop reagents for specific target antigens, such as orthologues of CD25 or CD45 isoforms.

Challenges and Research Directions in Equine Immunology

  • In terms of equine immunoglobulins, scientists must better establish the functional role of IgG isotypes and develop reagents for IgE.
  • The cloning of the immunoglobulin heavy chain genes is essential to unravel the complexities of the IgG sub-isotypes.
  • The report states that the challenges facing the relatively small global group of equine immunologists remain substantial and can only be effectively addressed through collaborative efforts.

Cite This Article

APA
Lunn DP, Holmes MA, Antczak DF, Agerwal N, Baker J, Bendali-Ahcene S, Blanchard-Channell M, Byrne KM, Cannizzo K, Davis W, Hamilton MJ, Hannant D, Kondo T, Kydd JH, Monier MC, Moore PF, O'Neil T, Schram BR, Sheoran A, Stott JL, Sugiura T, Vagnoni KE. (1998). Report of the Second Equine Leucocyte Antigen Workshop, Squaw valley, California, July 1995. Vet Immunol Immunopathol, 62(2), 101-143. https://doi.org/10.1016/s0165-2427(97)00160-8

Publication

Veterinary immunology and immunopathology
ISSN: 0165-2427
NlmUniqueID: 8002006
Country: Netherlands
Language: English
Volume: 62
Issue: 2
Pages: 101-143

Researcher Affiliations

Lunn, D P
  • School of Veterinary Medicine, University of Wisconsin, Madison 53706, USA. lunnp@svm.vetmed.wisc.edu
Holmes, M A
    Antczak, D F
      Agerwal, N
        Baker, J
          Bendali-Ahcene, S
            Blanchard-Channell, M
              Byrne, K M
                Cannizzo, K
                  Davis, W
                    Hamilton, M J
                      Hannant, D
                        Kondo, T
                          Kydd, J H
                            Monier, M C
                              Moore, P F
                                O'Neil, T
                                  Schram, B R
                                    Sheoran, A
                                      Stott, J L
                                        Sugiura, T
                                          Vagnoni, K E

                                            MeSH Terms

                                            • Animals
                                            • Histocompatibility Antigens / classification
                                            • Histocompatibility Antigens / immunology
                                            • Horses / immunology
                                            • Immunoglobulins / classification
                                            • Immunoglobulins / immunology
                                            • Immunologic Techniques

                                            Citations

                                            This article has been cited 33 times.
                                            1. Gressler AE, Lübke S, Wagner B, Arnold C, Lohmann KL, Schnabel CL. Comprehensive Flow Cytometric Characterization of Bronchoalveolar Lavage Cells Indicates Comparable Phenotypes Between Asthmatic and Healthy Horses But Functional Lymphocyte Differences.. Front Immunol 2022;13:896255.
                                              doi: 10.3389/fimmu.2022.896255pubmed: 35874777google scholar: lookup
                                            2. Schnabel CL, Fletemeyer B, Lübke S, Marti E, Wagner B, Alber G. CD154 Expression Indicates T Cell Activation Following Tetanus Toxoid Vaccination of Horses.. Front Immunol 2022;13:805026.
                                              doi: 10.3389/fimmu.2022.805026pubmed: 35493462google scholar: lookup
                                            3. Hagen A, Lehmann H, Aurich S, Bauer N, Melzer M, Moellerberndt J, Patané V, Schnabel CL, Burk J. Scalable Production of Equine Platelet Lysate for Multipotent Mesenchymal Stromal Cell Culture.. Front Bioeng Biotechnol 2020;8:613621.
                                              doi: 10.3389/fbioe.2020.613621pubmed: 33553119google scholar: lookup
                                            4. Patel RS, Tomlinson JE, Divers TJ, Van de Walle GR, Rosenberg BR. Single-cell resolution landscape of equine peripheral blood mononuclear cells reveals diverse cell types including T-bet(+) B cells.. BMC Biol 2021 Jan 22;19(1):13.
                                              doi: 10.1186/s12915-020-00947-5pubmed: 33482825google scholar: lookup
                                            5. Schnabel CL, Wimer CL, Perkins G, Babasyan S, Freer H, Watts C, Rollins A, Osterrieder N, Wagner B. Deletion of the ORF2 gene of the neuropathogenic equine herpesvirus type 1 strain Ab4 reduces virulence while maintaining strong immunogenicity.. BMC Vet Res 2018 Aug 22;14(1):245.
                                              doi: 10.1186/s12917-018-1563-4pubmed: 30134896google scholar: lookup
                                            6. Lee Y, Kiupel M, Soboll Hussey G. Characterization of respiratory dendritic cells from equine lung tissues.. BMC Vet Res 2017 Nov 6;13(1):313.
                                              doi: 10.1186/s12917-017-1240-zpubmed: 29110660google scholar: lookup
                                            7. Schwab UE, Tallmadge RL, Matychak MB, Felippe MJB. Effects of autologous stromal cells and cytokines on differentiation of equine bone marrow-derived progenitor cells.. Am J Vet Res 2017 Oct;78(10):1215-1228.
                                              doi: 10.2460/ajvr.78.10.1215pubmed: 28945121google scholar: lookup
                                            8. Hussen J, Shawaf T, Al-Herz AI, Alturaifi HR, Alluwaimi AM. Reactivity of commercially available monoclonal antibodies to human CD antigens with peripheral blood leucocytes of dromedary camels (Camelus dromedarius).. Open Vet J 2017;7(2):150-153.
                                              doi: 10.4314/ovj.v7i2.12pubmed: 28652982google scholar: lookup
                                            9. Prieto JMB, Tallmadge RL, Felippe MJB. Developmental expression of B cell molecules in equine lymphoid tissues.. Vet Immunol Immunopathol 2017 Jan;183:60-71.
                                              doi: 10.1016/j.vetimm.2016.12.004pubmed: 28063478google scholar: lookup
                                            10. Park KT, Seo KS, Godwin NA, Van Wie BJ, Gulbahar MY, Park YH, Davis WC. Characterization and expression of monoclonal antibody-defined molecules on resting and activated bovine αβ, γδ T and NK cells.. Vet Immunol Immunopathol 2015 Nov 15;168(1-2):118-30.
                                              doi: 10.1016/j.vetimm.2015.09.002pubmed: 26384699google scholar: lookup
                                            11. Badial PR, Tallmadge RL, Miller S, Stokol T, Richards K, Borges AS, Felippe MJ. Applied Protein and Molecular Techniques for Characterization of B Cell Neoplasms in Horses.. Clin Vaccine Immunol 2015 Nov;22(11):1133-45.
                                              doi: 10.1128/CVI.00374-15pubmed: 26311245google scholar: lookup
                                            12. Battista JM, Tallmadge RL, Stokol T, Felippe MJ. Hematopoiesis in the equine fetal liver suggests immune preparedness.. Immunogenetics 2014 Nov;66(11):635-49.
                                              doi: 10.1007/s00251-014-0799-9pubmed: 25179685google scholar: lookup
                                            13. Carrade DD, Lame MW, Kent MS, Clark KC, Walker NJ, Borjesson DL. Comparative Analysis of the Immunomodulatory Properties of Equine Adult-Derived Mesenchymal Stem Cells().. Cell Med 2012;4(1):1-11.
                                              doi: 10.3727/215517912X647217pubmed: 23152950google scholar: lookup
                                            14. Tallmadge RL, Stokol T, Gould-Earley MJ, Earley E, Secor EJ, Matychak MB, Felippe MJ. Fell Pony syndrome: characterization of developmental hematopoiesis failure and associated gene expression profiles.. Clin Vaccine Immunol 2012 Jul;19(7):1054-64.
                                              doi: 10.1128/CVI.00237-12pubmed: 22593239google scholar: lookup
                                            15. Soboll Hussey G, Hussey SB, Wagner B, Horohov DW, Van de Walle GR, Osterrieder N, Goehring LS, Rao S, Lunn DP. Evaluation of immune responses following infection of ponies with an EHV-1 ORF1/2 deletion mutant.. Vet Res 2011 Feb 7;42(1):23.
                                              doi: 10.1186/1297-9716-42-23pubmed: 21314906google scholar: lookup
                                            16. Wagner B, Burton A, Ainsworth D. Interferon-gamma, interleukin-4 and interleukin-10 production by T helper cells reveals intact Th1 and regulatory TR1 cell activation and a delay of the Th2 cell response in equine neonates and foals.. Vet Res 2010 Jul-Aug;41(4):47.
                                              doi: 10.1051/vetres/2010019pubmed: 20374696google scholar: lookup
                                            17. Go YY, Zhang J, Timoney PJ, Cook RF, Horohov DW, Balasuriya UB. Complex interactions between the major and minor envelope proteins of equine arteritis virus determine its tropism for equine CD3+ T lymphocytes and CD14+ monocytes.. J Virol 2010 May;84(10):4898-911.
                                              doi: 10.1128/JVI.02743-09pubmed: 20219931google scholar: lookup
                                            18. Paillot R, Robinson C, Steward K, Wright N, Jourdan T, Butcher N, Heather Z, Waller AS. Contribution of each of four Superantigens to Streptococcus equi-induced mitogenicity, gamma interferon synthesis, and immunity.. Infect Immun 2010 Apr;78(4):1728-39.
                                              doi: 10.1128/IAI.01079-09pubmed: 20123710google scholar: lookup
                                            19. de Mestre A, Noronha L, Wagner B, Antczak DF. Split immunological tolerance to trophoblast.. Int J Dev Biol 2010;54(2-3):445-55.
                                              doi: 10.1387/ijdb.082795adpubmed: 19876828google scholar: lookup
                                            20. Radcliffe CH, Flaminio MJ, Fortier LA. Temporal analysis of equine bone marrow aspirate during establishment of putative mesenchymal progenitor cell populations.. Stem Cells Dev 2010 Feb;19(2):269-82.
                                              doi: 10.1089/scd.2009.0091pubmed: 19604071google scholar: lookup
                                            21. Mealey RH, Leib SR, Littke MH, Wagner B, Horohov DW, McGuire TC. Viral load and clinical disease enhancement associated with a lentivirus cytotoxic T lymphocyte vaccine regimen.. Vaccine 2009 Apr 21;27(18):2453-68.
                                              doi: 10.1016/j.vaccine.2009.02.048pubmed: 19368787google scholar: lookup
                                            22. Fidalgo-Carvalho I, Craigo JK, Barnes S, Costa-Ramos C, Montelaro RC. Characterization of an equine macrophage cell line: application to studies of EIAV infection.. Vet Microbiol 2009 Apr 14;136(1-2):8-19.
                                              doi: 10.1016/j.vetmic.2008.10.010pubmed: 19038510google scholar: lookup
                                            23. Flaminio MJ, Tallmadge RL, Salles-Gomes CO, Matychak MB. Common variable immunodeficiency in horses is characterized by B cell depletion in primary and secondary lymphoid tissues.. J Clin Immunol 2009 Jan;29(1):107-16.
                                              doi: 10.1007/s10875-008-9221-4pubmed: 18677444google scholar: lookup
                                            24. Davis WC, Hamilton MJ. Use of flow cytometry to develop and characterize a set of monoclonal antibodies specific for rabbit leukocyte differentiation molecules.. J Vet Sci 2008 Mar;9(1):51-66.
                                              doi: 10.4142/jvs.2008.9.1.51pubmed: 18296889google scholar: lookup
                                            25. Mealey RH, Stone DM, Hines MT, Alperin DC, Littke MH, Leib SR, Leach SE, Hines SA. Experimental Rhodococcus equi and equine infectious anemia virus DNA vaccination in adult and neonatal horses: effect of IL-12, dose, and route.. Vaccine 2007 Oct 23;25(43):7582-97.
                                              doi: 10.1016/j.vaccine.2007.07.055pubmed: 17889970google scholar: lookup
                                            26. Mealey RH, Littke MH, Leib SR, Davis WC, McGuire TC. Failure of low-dose recombinant human IL-2 to support the survival of virus-specific CTL clones infused into severe combined immunodeficient foals: lack of correlation between in vitro activity and in vivo efficacy.. Vet Immunol Immunopathol 2008 Jan 15;121(1-2):8-22.
                                              doi: 10.1016/j.vetimm.2007.07.011pubmed: 17727961google scholar: lookup
                                            27. Lewis MJ, Wagner B, Woof JM. The different effector function capabilities of the seven equine IgG subclasses have implications for vaccine strategies.. Mol Immunol 2008 Feb;45(3):818-27.
                                              doi: 10.1016/j.molimm.2007.06.158pubmed: 17669496google scholar: lookup
                                            28. Mealey RH, Littke MH, Leib SR, Davis WC, McGuire TC. Cloning and large-scale expansion of epitope-specific equine cytotoxic T lymphocytes using an anti-equine CD3 monoclonal antibody and human recombinant IL-2.. Vet Immunol Immunopathol 2007 Jul 15;118(1-2):121-8.
                                              doi: 10.1016/j.vetimm.2007.04.001pubmed: 17498813google scholar: lookup
                                            29. Flaminio MJ, Borges AS, Nydam DV, Horohov DW, Hecker R, Matychak MB. The effect of CpG-ODN on antigen presenting cells of the foal.. J Immune Based Ther Vaccines 2007 Jan 25;5:1.
                                              doi: 10.1186/1476-8518-5-1pubmed: 17254326google scholar: lookup
                                            30. Cunha CW, McGuire TC, Kappmeyer LS, Hines SA, Lopez AM, Dellagostin OA, Knowles DP. Development of specific immunoglobulin Ga (IgGa) and IgGb antibodies correlates with control of parasitemia in Babesia equi Infection.. Clin Vaccine Immunol 2006 Feb;13(2):297-300.
                                              doi: 10.1128/CVI.13.2.297-300.2006pubmed: 16467341google scholar: lookup
                                            31. Mérant C, Messouak A, Cadoré JL, Monier JC. PNA-binding glycans are expressed at high levels on horse mature and immature T lymphocytes and a subpopulation of B lymphocytes.. Glycoconj J 2005 Feb;22(1-2):27-34.
                                              doi: 10.1007/s10719-005-0228-2pubmed: 15864432google scholar: lookup
                                            32. Patton KM, McGuire TC, Fraser DG, Hines SA. Rhodococcus equi-infected macrophages are recognized and killed by CD8+ T lymphocytes in a major histocompatibility complex class I-unrestricted fashion.. Infect Immun 2004 Dec;72(12):7073-83.
                                              doi: 10.1128/IAI.72.12.7073-7083.2004pubmed: 15557631google scholar: lookup
                                            33. Flock M, Jacobsson K, Frykberg L, Hirst TR, Franklin A, Guss B, Flock JI. Recombinant Streptococcus equi proteins protect mice in challenge experiments and induce immune response in horses.. Infect Immun 2004 Jun;72(6):3228-36.
                                              doi: 10.1128/IAI.72.6.3228-3236.2004pubmed: 15155624google scholar: lookup
                                            Subscribe to our newsletter
                                            Join 99,344 horse owners like you who receive our email updates on horse health and nutrition.