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Home / Equine Research Bank / Res Vet Sci / Responses of ponies to equid herpesvirus-1 ISCOM vaccination...
Research in veterinary science1993; 54(3); 299-305; doi: 10.1016/0034-5288(93)90126-z

Responses of ponies to equid herpesvirus-1 ISCOM vaccination and challenge with virus of the homologous strain.

Abstract: An experimental (ISCOM) vaccine previously shown to protect hamsters from lethal challenge with equid herpesvirus-1 (EHV-1), was tested in horses. Vaccination with EHV-1 ISCOMs induced serum antibodies to the major virus glycoproteins gp10, 13, 14, 17, 18 and 21/22a, whereas antibody responses to gp2 were weak or absent. High levels of virus neutralising antibody of long duration were induced, but did not prevent challenge infection with virus of the homologous strain. However, in the vaccinated ponies there was a significant reduction in clinical signs, nasal virus excretion and cell associated viraemia compared with age-matched unvaccinated controls. There was a strong correlation between pre-challenge levels of serum virus neutralising antibody and the duration and total amount of virus excreted from the nasopharynx.
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Publication Date: 1993-05-01 PubMed ID: 8393207DOI: 10.1016/0034-5288(93)90126-zGoogle Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research tests an experimental vaccine, previously established to protect hamsters from the equid herpesvirus-1 (EHV-1), on horses. Though it did not fully prevent infection, the EHV-1 ISCOM vaccine led to a significant decrease in clinical symptoms, virus excretion and viraemia in vaccinated horses, and also induced long-lasting antibodies.

Experimental EHV-1 Vaccine

  • The ISCOM (Immunostimulatory complex) vaccine was initially shown to be effective in protecting hamsters from equid herpesvirus-1, a virus that can infect members of the equine family.
  • This study attempts to extend previous findings and test the effectiveness of the ISCOM vaccine in actual equines, specifically ponies.

Vaccine Effects on Immune Response

  • Following vaccination with the EHV-1 ISCOM vaccine, the ponies produced serum antibodies against several key virus-associated glycoproteins (gp10, 13, 14, 17, 18, and 21/22a).
  • However, the vaccine did not seem to elicit a strong antibody response against the gp2 protein, suggesting this protein might not be a key target for the ISCOM vaccine.
  • The vaccine induced high levels of virus-neutralising antibodies and these antibodies persisted for a long duration.

Outcomes of Virus Challenge Post Vaccination

  • Despite the strong production of virus-neutralizing antibodies, the EHV-1 ISCOM vaccinated ponies could not completely resist subsequent infection with the homologous virus strain.
  • However, the severity of disease symptoms were significantly reduced in the vaccinated ponies compared to unvaccinated controls. Specifically, there were fewer clinical signs, reduced nasal virus excretion, and less cell-associated viraemia.
  • The level of virus-neutralising antibodies in the ponies’ serum prior to exposure to the virus correlated strongly with the duration and total amount of virus excreted from the nasopharynx. This suggests that higher levels of these antibodies may confer enhanced protection against the virus.

Cite This Article

APA
Hannant D, Jessett DM, O'Neill T, Dolby CA, Cook RF, Mumford JA. (1993). Responses of ponies to equid herpesvirus-1 ISCOM vaccination and challenge with virus of the homologous strain. Res Vet Sci, 54(3), 299-305. https://doi.org/10.1016/0034-5288(93)90126-z

Publication

Research in veterinary science
ISSN: 0034-5288
NlmUniqueID: 0401300
Country: England
Language: English
Volume: 54
Issue: 3
Pages: 299-305

Researcher Affiliations

Hannant, D
  • Department of Infectious Diseases, Animal Health Trust, Newmarket, Suffolk.
Jessett, D M
    O'Neill, T
      Dolby, C A
        Cook, R F
          Mumford, J A

            MeSH Terms

            • Animals
            • Antibodies, Viral / blood
            • Antibody Formation
            • Herpesviridae / immunology
            • Herpesviridae / isolation & purification
            • Herpesviridae Infections / immunology
            • Herpesviridae Infections / veterinary
            • Horse Diseases
            • Horses / immunology
            • ISCOMs / administration & dosage
            • Neutralization Tests
            • Viral Matrix Proteins / immunology
            • Viral Vaccines / administration & dosage

            Citations

            This article has been cited 17 times.
            1. Carnet F, Perrin-Cocon L, Paillot R, Lotteau V, Pronost S, Vidalain PO. An inventory of adjuvants used for vaccination in horses: the past, the present and the future. Vet Res 2023 Mar 2;54(1):18.
              doi: 10.1186/s13567-023-01151-3pubmed: 36864517google scholar: lookup
            2. El Brini Z, Fassi Fihri O, Paillot R, Lotfi C, Amraoui F, El Ouadi H, Dehhaoui M, Colitti B, Alyakine H, Piro M. Seroprevalence of Equine Herpesvirus 1 (EHV-1) and Equine Herpesvirus 4 (EHV-4) in the Northern Moroccan Horse Populations. Animals (Basel) 2021 Sep 29;11(10).
              doi: 10.3390/ani11102851pubmed: 34679874google scholar: lookup
            3. Zarski LM, Vaala WE, Barnett DC, Bain FT, Soboll Hussey G. A Live-Attenuated Equine Influenza Vaccine Stimulates Innate Immunity in Equine Respiratory Epithelial Cell Cultures That Could Provide Protection From Equine Herpesvirus 1. Front Vet Sci 2021;8:674850.
              doi: 10.3389/fvets.2021.674850pubmed: 34179166google scholar: lookup
            4. Di Francesco CE, Smoglica C, De Amicis I, Cafini F, Carluccio A, Contri A. Evaluation of Colostral Immunity Against Equine Herpesvirus Type 1 (EHV-1) in Martina Franca's Foals. Front Vet Sci 2020;7:579371.
              doi: 10.3389/fvets.2020.579371pubmed: 33330699google scholar: lookup
            5. Oladunni FS, Horohov DW, Chambers TM. EHV-1: A Constant Threat to the Horse Industry. Front Microbiol 2019;10:2668.
              doi: 10.3389/fmicb.2019.02668pubmed: 31849857google scholar: lookup
            6. Kim SK, Shakya AK, O'Callaghan DJ. Intranasal treatment with CpG-B oligodeoxynucleotides protects CBA mice from lethal equine herpesvirus 1 challenge by an innate immune response. Antiviral Res 2019 Sep;169:104546.
              doi: 10.1016/j.antiviral.2019.104546pubmed: 31247247google scholar: lookup
            7. Liu SA, Stanfield BA, Chouljenko VN, Naidu S, Langohr I, Del Piero F, Ferracone J, Roy AA, Kousoulas KG. Intramuscular Immunization of Mice with the Live-Attenuated Herpes Simplex Virus 1 Vaccine Strain VC2 Expressing Equine Herpesvirus 1 (EHV-1) Glycoprotein D Generates Anti-EHV-1 Immune Responses in Mice. J Virol 2017 Jun 15;91(12).
              doi: 10.1128/JVI.02445-16pubmed: 28404844google scholar: lookup
            8. Kim SK, Shakya AK, O'Callaghan DJ. Immunization with Attenuated Equine Herpesvirus 1 Strain KyA Induces Innate Immune Responses That Protect Mice from Lethal Challenge. J Virol 2016 Sep 15;90(18):8090-104.
              doi: 10.1128/JVI.00986-16pubmed: 27356904google scholar: lookup
            9. Bergmann T, Moore C, Sidney J, Miller D, Tallmadge R, Harman RM, Oseroff C, Wriston A, Shabanowitz J, Hunt DF, Osterrieder N, Peters B, Antczak DF, Sette A. The common equine class I molecule Eqca-1*00101 (ELA-A3.1) is characterized by narrow peptide binding and T cell epitope repertoires. Immunogenetics 2015 Nov;67(11-12):675-89.
              doi: 10.1007/s00251-015-0872-zpubmed: 26399241google scholar: lookup
            10. Goodman LB, Wimer C, Dubovi EJ, Gold C, Wagner B. Immunological correlates of vaccination and infection for equine herpesvirus 1. Clin Vaccine Immunol 2012 Feb;19(2):235-41.
              doi: 10.1128/CVI.05522-11pubmed: 22205656google scholar: lookup
            11. Soboll Hussey G, Hussey SB, Wagner B, Horohov DW, Van de Walle GR, Osterrieder N, Goehring LS, Rao S, Lunn DP. Evaluation of immune responses following infection of ponies with an EHV-1 ORF1/2 deletion mutant. Vet Res 2011 Feb 7;42(1):23.
              doi: 10.1186/1297-9716-42-23pubmed: 21314906google scholar: lookup
            12. Smith PM, Kahan SM, Rorex CB, von Einem J, Osterrieder N, O'Callaghan DJ. Expression of the full-length form of gp2 of equine herpesvirus 1 (EHV-1) completely restores respiratory virulence to the attenuated EHV-1 strain KyA in CBA mice. J Virol 2005 Apr;79(8):5105-15.
              doi: 10.1128/JVI.79.8.5105-5115.2005pubmed: 15795295google scholar: lookup
            13. Smith PM, Zhang Y, Grafton WD, Jennings SR, O'Callaghan DJ. Severe murine lung immunopathology elicited by the pathogenic equine herpesvirus 1 strain RacL11 correlates with early production of macrophage inflammatory proteins 1alpha, 1beta, and 2 and tumor necrosis factor alpha. J Virol 2000 Nov;74(21):10034-40.
              doi: 10.1128/jvi.74.21.10034-10040.2000pubmed: 11024132google scholar: lookup
            14. Smith PM, Zhang Y, Jennings SR, O'Callaghan DJ. Characterization of the cytolytic T-lymphocyte response to a candidate vaccine strain of equine herpesvirus 1 in CBA mice. J Virol 1998 Jul;72(7):5366-72.
              doi: 10.1128/JVI.72.7.5366-5372.1998pubmed: 9620990google scholar: lookup
            15. Hu Y, Wu G, Jia Q, Zhang B, Sun W, Sa R, Zhang S, Cai W, Jarhen, Ran D, Liu J. Development of a live attenuated vaccine candidate for equid alphaherpesvirus 1 control: a step towards efficient protection. Front Immunol 2024;15:1408510.
              doi: 10.3389/fimmu.2024.1408510pubmed: 39021566google scholar: lookup
            16. Pusterla N, Dorman DC, Burgess BA, Goehring L, Gross M, Osterrieder K, Soboll Hussey G, Lunn DP. Viremia and nasal shedding for the diagnosis of equine herpesvirus-1 infection in domesticated horses. J Vet Intern Med 2024 May-Jun;38(3):1765-1791.
              doi: 10.1111/jvim.16958pubmed: 38069548google scholar: lookup
            17. Osterrieder K, Dorman DC, Burgess BA, Goehring LS, Gross P, Neinast C, Pusterla N, Hussey GS, Lunn DP. Vaccination for the prevention of equine herpesvirus-1 disease in domesticated horses: A systematic review and meta-analysis. J Vet Intern Med 2024 May-Jun;38(3):1858-1871.
              doi: 10.1111/jvim.16895pubmed: 37930113google scholar: lookup
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