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Research in veterinary science1993; 54(3); 299-305; doi: 10.1016/0034-5288(93)90126-z

Responses of ponies to equid herpesvirus-1 ISCOM vaccination and challenge with virus of the homologous strain.

Abstract: An experimental (ISCOM) vaccine previously shown to protect hamsters from lethal challenge with equid herpesvirus-1 (EHV-1), was tested in horses. Vaccination with EHV-1 ISCOMs induced serum antibodies to the major virus glycoproteins gp10, 13, 14, 17, 18 and 21/22a, whereas antibody responses to gp2 were weak or absent. High levels of virus neutralising antibody of long duration were induced, but did not prevent challenge infection with virus of the homologous strain. However, in the vaccinated ponies there was a significant reduction in clinical signs, nasal virus excretion and cell associated viraemia compared with age-matched unvaccinated controls. There was a strong correlation between pre-challenge levels of serum virus neutralising antibody and the duration and total amount of virus excreted from the nasopharynx.
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Publication Date: 1993-05-01 PubMed ID: 8393207DOI: 10.1016/0034-5288(93)90126-zGoogle Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research tests an experimental vaccine, previously established to protect hamsters from the equid herpesvirus-1 (EHV-1), on horses. Though it did not fully prevent infection, the EHV-1 ISCOM vaccine led to a significant decrease in clinical symptoms, virus excretion and viraemia in vaccinated horses, and also induced long-lasting antibodies.

Experimental EHV-1 Vaccine

  • The ISCOM (Immunostimulatory complex) vaccine was initially shown to be effective in protecting hamsters from equid herpesvirus-1, a virus that can infect members of the equine family.
  • This study attempts to extend previous findings and test the effectiveness of the ISCOM vaccine in actual equines, specifically ponies.

Vaccine Effects on Immune Response

  • Following vaccination with the EHV-1 ISCOM vaccine, the ponies produced serum antibodies against several key virus-associated glycoproteins (gp10, 13, 14, 17, 18, and 21/22a).
  • However, the vaccine did not seem to elicit a strong antibody response against the gp2 protein, suggesting this protein might not be a key target for the ISCOM vaccine.
  • The vaccine induced high levels of virus-neutralising antibodies and these antibodies persisted for a long duration.

Outcomes of Virus Challenge Post Vaccination

  • Despite the strong production of virus-neutralizing antibodies, the EHV-1 ISCOM vaccinated ponies could not completely resist subsequent infection with the homologous virus strain.
  • However, the severity of disease symptoms were significantly reduced in the vaccinated ponies compared to unvaccinated controls. Specifically, there were fewer clinical signs, reduced nasal virus excretion, and less cell-associated viraemia.
  • The level of virus-neutralising antibodies in the ponies’ serum prior to exposure to the virus correlated strongly with the duration and total amount of virus excreted from the nasopharynx. This suggests that higher levels of these antibodies may confer enhanced protection against the virus.

Cite This Article

APA
Hannant D, Jessett DM, O'Neill T, Dolby CA, Cook RF, Mumford JA. (1993). Responses of ponies to equid herpesvirus-1 ISCOM vaccination and challenge with virus of the homologous strain. Res Vet Sci, 54(3), 299-305. https://doi.org/10.1016/0034-5288(93)90126-z

Publication

Research in veterinary science
ISSN: 0034-5288
NlmUniqueID: 0401300
Country: England
Language: English
Volume: 54
Issue: 3
Pages: 299-305

Researcher Affiliations

Hannant, D
  • Department of Infectious Diseases, Animal Health Trust, Newmarket, Suffolk.
Jessett, D M
    O'Neill, T
      Dolby, C A
        Cook, R F
          Mumford, J A

            MeSH Terms

            • Animals
            • Antibodies, Viral / blood
            • Antibody Formation
            • Herpesviridae / immunology
            • Herpesviridae / isolation & purification
            • Herpesviridae Infections / immunology
            • Herpesviridae Infections / veterinary
            • Horse Diseases
            • Horses / immunology
            • ISCOMs / administration & dosage
            • Neutralization Tests
            • Viral Matrix Proteins / immunology
            • Viral Vaccines / administration & dosage

            Citations

            This article has been cited 17 times.
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