Analyze Diet
Journal of advanced veterinary and animal research2022; 9(2); 323-334; doi: 10.5455/javar.2022.i599

Review of anthrax: A disease of farm animals.

Abstract: Anthrax is a rapidly fatal infectious disease affecting herbivores and people. In the farm animals, cattle and sheep are more susceptible, followed by goats and horses, while dwarf pigs and Algerian sheep are relatively resistant. Bacillus anthracis, the causative agent of anthrax, produces spores and persists for decades in the soil, initiating an outbreak through a favorable climate shift. Anthrax is enzootic in many Asian and African countries, and is reported in Australia, some parts of Europe, and America. The clinical courses of this disease in animals are peracute, acute, subacute, and chronic forms. In severely infected cases, the animals are dead without premonitory clinical signs. The blood may fail to clot and can be found in the mouth, nostrils, and anus in the animals that die from anthrax. This bacterium is susceptible to many antibiotics, yet only penicillin and oxytetracycline have the most effective under field conditions. When an outbreak occurs in a defined area, it is necessary to take early steps to break the infection cycle by maintaining strict biosecurity and vaccinating uninfected animals. This disease is still a challenge to farm animal production in many countries. This review intends to give a fair knowledge of the etiology, epidemiology, pathogenesis, clinical presentation, diagnosis, treatment, and control of this disease.
Publication Date: 2022-06-30 PubMed ID: 35891653PubMed Central: PMC9298093DOI: 10.5455/javar.2022.i599Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
  • Journal Article
  • Review

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research paper discusses the complex aspects of anthrax, an infectious disease that primarily affects farm animals, its epidemiology, pathogenesis, clinical presentation, diagnosis, treatment, and control strategies.

Etiology and Epidemiology

  • Anthrax is a fatal disease primarily affecting herbivores such as cattle, sheep, goats, and horses. It is caused by a bacterium that releases spores that can survive for decades in soil.
  • The disease is prevalent in many countries across Africa and Asia, with some documented cases in Australia, Europe, and the Americas.

Pathogenesis and Clinical Presentation

  • The disease exhibits several forms depending on its severity – peracute, acute, subacute, and chronic.
  • In severe cases, animals might die without showing explicit clinical signs, while in other cases, their blood fails to clot, and it can be seen near the mouth, nostrils, and anus.

Diagnosis and Treatment

  • Diagnosis is based on clinical presentation and the manifestation of specific symptoms—in particular the failing clotting of blood in affected animals.
  • The bacteria causing anthrax are susceptible to several antibiotics, but penicillin and oxytetracycline have been identified as the most effective in field conditions.

Control Measures

  • In areas where an outbreak occurs, immediate measures should be taken to disrupt the cycle of infection. These measures include maintaining strict biosecurity and vaccinating uninfected animals.
  • Despite these control measures, anthrax remains a challenge to farm animal production in numerous countries due to its rapid spread and high mortality rate.

Overall, this paper provides a comprehensive overview of anthrax, offering important insights into its cause, effects, treatment, and ways to control its spread. It affirms the necessity for ongoing research and effective control measures to combat this fatal disease that threatens farm animal production worldwide.

Cite This Article

APA
Alam ME, Kamal MM, Rahman M, Kabir A, Islam MS, Hassan J. (2022). Review of anthrax: A disease of farm animals. J Adv Vet Anim Res, 9(2), 323-334. https://doi.org/10.5455/javar.2022.i599

Publication

ISSN: 2311-7710
NlmUniqueID: 101647585
Country: Bangladesh
Language: English
Volume: 9
Issue: 2
Pages: 323-334

Researcher Affiliations

Alam, Md Emtiaj
  • Department of Veterinary and Animal Sciences, Faculty of Veterinary and Animal Sciences, University of Rajshahi, Rajshahi, Bangladesh.
Kamal, Md Mostofa
  • Department of Veterinary and Animal Sciences, Faculty of Veterinary and Animal Sciences, University of Rajshahi, Rajshahi, Bangladesh.
Rahman, Moizur
  • Department of Veterinary and Animal Sciences, Faculty of Veterinary and Animal Sciences, University of Rajshahi, Rajshahi, Bangladesh.
Kabir, Aurangazeb
  • Department of Veterinary and Animal Sciences, Faculty of Veterinary and Animal Sciences, University of Rajshahi, Rajshahi, Bangladesh.
Islam, Md Shafiqul
  • Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh.
Hassan, Jayedul
  • Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh.

Conflict of Interest Statement

The authors declare that they have no potential conflict of interest in this review.

References

This article includes 83 references
  1. OIE. 6th. Paris, France: International Des Epizooties; 2008. Manual of diagnostic tests and vaccines for terrestrial animals; pp. 599–1341.
  2. Fasanella A, Garofolo G, Galante D, Quaranta V, Palazzo L, Lista F. Severe anthrax outbreaks in Italy in 2004: considerations on factors involved in the spread of infection. New Microbiol 2010;33(1):83–6.
    pubmed: 20402418
  3. Minett F, Dhanda M. Multiplication of B. anthracis and Cl. chauvei in soil and water. Ind J Vet Sci Anim Husb 1941;11:308–28.
  4. Hugh-Jones ME, De Vos V. Anthrax and wildlife. Revue Scientifique et Technique-Office International des Epizooties 2002;21(2):359–83.
    pubmed: 11974621doi: 10.20506/rst.21.2.1336google scholar: lookup
  5. Lindeque PM, Turnbull PCB. Ecology and epidemiology of Anthrax in the Etosha National Park, Namibia. Onderstepoort J Vet Res 1994;61:71–83.
    pubmed: 7898901
  6. Titball RW, Turnbull PCB, Hutson RA. The monitoring and detection of Bacillus anthracis in the environment. Soc Appl Bacteriol Symp Ser 1991;20:9S–18S.
    pubmed: 1909461
  7. Davies D. The influence of temperature and humidity on spore formation and germination in Bacillus anthracis. Epidemiol Infect 1960;58:177–86.
    pmc: PMC2134342pubmed: 20475844doi: 10.1017/s0022172400038250google scholar: lookup
  8. Quinn C, Turnbull P. Anthrax. Topley and Wilson’s microbiology and microbial infections London, UK: Edward Ernold; 1998. Contributions to the Bacterial Infections; pp. 799–818.
  9. Constable P, Hinchcliff K w, Done S, Gruenberg W. Anthrax. Veterinary medicine 11th. Elsevier; 2016; pp. 1–2235.
  10. Dragon DC, Rennie RP. The ecology of anthrax spores: tough but not invincible. Can Vet J 1995;36(5):295–301.
    pmc: PMC1686874pubmed: 7773917
  11. Golsteyn-Thomas E, Gale S. Anthrax. Contribution to the Infectious and parasitic diseases of livestock Wallingford, UK: 2010; pp. 1197–206.
  12. Mongoh MN, Dyer NW, Stoltenow CL, Khaitsa ML. Risk factors associated with anthrax outbreak in animals in North Dakota, 2005: a retrospective case-control study. Pub Health Rep 2008;123(3):352–9.
    pmc: PMC2289988pubmed: 19006977doi: 10.1177/003335490812300315google scholar: lookup
  13. Durrheim DN, Freeman P, Roth I, Hornitzky M. Epidemiologie questions from anthrax outbreak, Hunter Valley, Australia. Emerg Infect Dis 2009;15(5):840–2.
    pmc: PMC2687039pubmed: 19402992doi: 10.3201/eid1505.081744google scholar: lookup
  14. Lewerin SS, Elvander M, Westermark T, Hartzell LN, Norström AK, Ehrs S. Anthrax outbreak in a Swedish beef cattle herd- 1st case in 27 years: case report. Acta Vet Scand 2010;52(1):1–8.
    pmc: PMC2826306pubmed: 20122147doi: 10.1186/1751-0147-52-7google scholar: lookup
  15. Ahmed BN, Sultana Y, Fatema D, Ara K, Begum N, Mostanzid S. Anthrax: an emerging zoonotic disease in Bangladesh. Bang J Med Microbiol 2010;4(1):46–50.
    doi: 10.3329/bjmm.v4i1.8470google scholar: lookup
  16. Fasanella A, Garofolo G, Hossain MJ, Shamsuddin M, Blackburn JK, Hugh-jones M. Bangladesh anthrax outbreaks are probably caused by contaminated livestock feed. Epidemiol Infect 2013;141:1021–8.
    pmc: PMC9151811pubmed: 22814512doi: 10.1017/s0950268812001227google scholar: lookup
  17. Mustafa AH. Isolation of anthrax bacillus from an elephant in Bangladesh. Vet Record 1984;114(24):590.
    pubmed: 6431687doi: 10.1136/vr.114.24.590google scholar: lookup
  18. Samad MA, Hoque ME. Anthrax in man and cattle in Bangladesh. J Trop Med Hyg 1986;89(1):43–5.
    pubmed: 3746993
  19. American Veterinary Medical Association (AVMA). Anthrax facts, 2001. [December 10; 2020]. Available via https://www.av ma.org/anthrax-facts.
  20. WHO. Anthrax in humans and animals. Geneva, Switzerland: WHO Press; 2008; pp. 1–198.
  21. NSW GOVERNMENT. Anthrax. [April 12; 2022]. Available via https://www.dpi.nsw.gov.au/biosecurity/animals/Humans/anthrax.
  22. United States Department of Labour. Anthrax. [April 12; 2022]. Available via https://www.osha.gov/anthrax/control-prevention#ftn2.
  23. CDC. Anthrax. [December 11; 2021]. Available via https://www.cdc.gov/anthrax/basics/anthrax-history.html.
  24. Raymond B, Wyres KL, Sheppard SK, Ellis RJ, Bonsall MB. Environmental factors determining the epidemiology and population genetic structure of the Bacillus cereus group in the field. PLoS Path 2010:6–5. e1000905.
  25. Wikipedia. Bacillus anthracis. [February 15; 2022]. Avaialble via https://en.wikipedia.org/wiki/Bacillus_anthracis.
  26. Demicheli V, Rivetti D, Deeks JJ, Jefferson T, Pratt M. The effectiveness and safety of Vaccines against human anthrax: a systematic review. Vaccine 1998;16:880–4.
    pubmed: 9682332doi: 10.1016/s0264-410x(98)00023-1google scholar: lookup
  27. Cummings RT, Salowe SP, Cunningham BR, Wiltsie J, Park YW, Sonatore LK. A peptide-based fluorescence resonance energy transfer assay for Bacillus anthracis lethal factor protease. Proc Natl Acad Sci USA 2002;99(10):6603–6.
    pmc: PMC124449pubmed: 11997440doi: 10.1073/pnas.062171599google scholar: lookup
  28. Wikipedia. Vollum strain. 2021. [February 8; 2021]. Available via https://en.wikipedia.org/wiki/Vollum_strain.
  29. Wilson JM, Brediger W, Albright TP, Smith-Gagen J. Reanalysis of the anthrax epidemic in Rhodesia, 1978–1984. PeerJ 2016;4:e2686.
    pmc: PMC5111893pubmed: 27867766doi: 10.7717/peerj.2686google scholar: lookup
  30. Jernigan JA, Stephens DS, Ashford DA, Omenaca C, Topiel MS, Galbraith M. Bioterrorism-related inhalational anthrax: the first 10 cases reported in the United States. Emerg Infect Dis 2001;7(6):933–44.
    pmc: PMC2631903pubmed: 11747719doi: 10.3201/eid0706.010604google scholar: lookup
  31. Ngetich W. Review of anthrax: a disease of animals and humans. Int J Agric Environ Biores 2019;4:123–34.
  32. Hornitzky M, Muller J. Anthrax. Canberra, Australia: 2010. [February 9; 2021]. Australian Government Department of Agriculture, Water and the Environment; pp. 1–15. Available via https://www.agriculture.gov.au/animal/health/laboratories/procedures/anzsdp/anthrax.
  33. Schwartz M, Jekyll DR, Hyde MR. A short history of anthrax. Mol Asp Med 2009;30:347–55.
    pubmed: 19577591doi: 10.1016/j.mam.2009.06.004google scholar: lookup
  34. Abrami L, Reig N, van der Goot FG. Anthrax toxin: the long and winding road that leads to the kill. Trends Microbiol 2005;13(2):72–8.
    pubmed: 15680766doi: 10.1016/j.tim.2004.12.004google scholar: lookup
  35. Kim C, Wilcox-Adelman S, Sano Y, Tang WJ, Collier RJ, Jin MP. Antiinflammatory cAMP signaling and cell migration genes co-opted by the anthrax bacillus. Proc Natl Acad Sci USA 2008;105(16):6150–5.
    pmc: PMC2329691pubmed: 18427110doi: 10.1073/pnas.0800105105google scholar: lookup
  36. Leppla SH. Anthrax toxin edema factor: a bacterial adenylate cyclase that increases cyclic AMP concentrations in eukaryotic cells. Proc Natl Acad Sci USA 1982;79(10):3162–6.
    pmc: PMC346374pubmed: 6285339doi: 10.1073/pnas.79.10.3162google scholar: lookup
  37. Jamie WE. Anthrax: Diagnosis, treatment, prevention. Prim Care Upd Ob/Gyns 2002;9(4):117–21.
  38. Goossens PL. Animal models of human anthrax: the Quest for the Holy Grail. Mol Aspects Med 2009;30:467–80.
    pubmed: 19665473doi: 10.1016/j.mam.2009.07.005google scholar: lookup
  39. Hassan J, Murshidul Ahsan M, Bahanur Rahman M, Md Ziqrul Haq Chowdhury S, Shafiullah Parvej M. Factors associated with repeated outbreak of anthrax in Bangladesh: qualitative and quantitative study. J Adv Vet Anim Res 2015;2(2):158–64.
    doi: 10.5455/javar.2015.b72google scholar: lookup
  40. Garofolo G, Ciammaruconi A, Fasanella A, Scasciamacchia S, Adone R, Pittiglio V. SNR analysis: molecular investigation of an anthrax epidemic. BMC Vet Res 2010;6(11):1–8.
    pmc: PMC2837646pubmed: 20187980doi: 10.1186/1746-6148-6-11google scholar: lookup
  41. New M, Lister D, Hulme M, Makin I. A high-resolution data set of surface climate over global land areas. Clim Res 2002;21(1):1–25.
    doi: 10.3354/cr021001google scholar: lookup
  42. Baril E, Coroller L, Couvert O, Leguérinel I, Postollec F, Boulais C. Modeling heat resistance of Bacillus weihenstephanensis and Bacillus licheniformis spores as function of sporulation temperature and pH. Food Microbiol 2012;30(1):29–36.
    pubmed: 22265280doi: 10.1016/j.fm.2011.09.017google scholar: lookup
  43. Baweja RB, Zaman MS, Mattoo AR, Sharma K, Tripathi V, Aggarwal A. Properties of Bacillus anthracis spores prepared under various environmental conditions. Arch Microbiol 2008;189(1):71–9.
    pubmed: 17713759doi: 10.1007/s00203-007-0295-9google scholar: lookup
  44. Bressuire-Isoard C, Broussolle V, Carlin F. Sporulation environment influences spore properties in Bacillus: evidence and insights on underlying molecular and physiological mechanisms. FEMS Microbiol Rev 2018;42(5):614–26.
    pubmed: 29788151doi: 10.1093/femsre/fuy021google scholar: lookup
  45. Mazas M, Lopez M, Gonzalez I, Bernardo A, Martin R. Effects of sporulation pH on the heat resistance and the sporulation of Bacillus cereus. Lett Appl Microbiol 1997;25(5):331–4.
  46. Minh HNT, Durand A, Loison P, Perrier-Cornet JM, Gervais P. Effect of sporulation conditions on the resistance of Bacillus subtilis spores to heat and high pressure. Appl Microbiol Biotechnol 2011;90(4):1409–17.
    pubmed: 21380515doi: 10.1007/s00253-011-3183-9google scholar: lookup
  47. Planchon S, Dargaignaratz C, Levy C, Ginies C, Broussolle V, Carlin F. Spores of Bacillus cereus strain KBAB4 produced at 10oC and 30o C display variations in their properties. Food Microbiol 2011;28(2):291–7.
    pubmed: 21315986doi: 10.1016/j.fm.2010.07.015google scholar: lookup
  48. Hugh-Jones M, Blackburn J. The ecology of Bacillus anthracis. Mol Asp Med 2009;30(6):356–67.
    pubmed: 19720074doi: 10.1016/j.mam.2009.08.003google scholar: lookup
  49. Widderich N, Rodrigues CD, Commichau FM, Fischer KE, Ramirez‐Guadiana FH, Rudner DZ. Salt‐sensitivity of σH and Spo0A prevents sporulation of Bacillus subtilis at high osmolarity avoiding death during cellular differentiation. Mol Microbiol 2016;100(1):108–24.
    pmc: PMC4992981pubmed: 26712348doi: 10.1111/mmi.13304google scholar: lookup
  50. Abbas AA, Planchon S, Jobin M, Schmitt P. Absence of oxygen affects the capacity to sporulate and the spore properties of Bacillus cereus. Food Microbiol 2014;42:122–31.
    pubmed: 24929727
  51. Avignone-Rossa C, Arcas J, Mignone C. Bacillus thuringiensis growth, sporulation and δ-endotoxin production in oxygen limited and non-limited cultures. World J Microbiol Biotechnol 1992;8(3):301–4.
    pubmed: 24425483
  52. Boniolo FS, Rodrigues RC, Prata AM, López ML, Jacinto T, da Silveira MM, Berbert-Molina MA. Oxygen supply in Bacillus thuringiensis fermentations: bringing new insights on their impact on sporulation and δ-endotoxin production. Appl Microbiol Biotechnol 2012;94(3):625–36.
    pubmed: 22395904
  53. Hilmas CJ, Katos AM, Williams PT, Anderson J. Anthrax. Handbook of toxicology of chemical warfare agents Cambridge, MA: Academic Press; 2009; pp. 433–59.
  54. Driks A. The Bacillus anthracis spore. Mol Asp Med 2009;30(6):368–73.
    pubmed: 19683018doi: 10.1016/j.mam.2009.08.001google scholar: lookup
  55. Inglesby TV, O’Toole T, Henderson DA, Bartlett JG, Ascher MS, Eitzen E. Anthrax as a biological weapon, 2002 updated recommendations for management. J Am Med Assoc 2002;287(17):2236–52.
    pubmed: 11980524doi: 10.1001/jama.287.17.2236google scholar: lookup
  56. Centre for Health Security. Bacillus anthracis (Anthrax) 2014. [February 15; 2022]. Available via https://www.centerforhealthsecurity.org/our-work/publications/bacillus-anthracis-anthrax-fact-.sheet.
  57. Dixon TC, Meselson M, Guillemin J, Hanna PC. Anthrax. New Eng J Med 1999;341:815–26.
    pubmed: 10477781doi: 10.1056/nejm199909093411107google scholar: lookup
  58. Williams R. Bacillus anthracis and other spore forming bacilli. Contributions to the infectious disease and medical microbiology Philadelphia, PA: WB Saunders Co; 1986; pp. 270–8.
  59. Friedlander A. Tackling anthrax. Nature 2001;414:160–1.
    pubmed: 11700539doi: 10.1038/35102660google scholar: lookup
  60. Kau JH, Sun DS, Tsai WJ, Shyu HF, Huang HH, Lin HC. Antiplatelet activities of anthrax lethal toxin are associated with suppressed p42/44 and p38 mitogen-activated protein kinase pathways in the platelets. J Infect Dis 2005;192(8):1465–74.
    pubmed: 16170766
  61. Schlingman AS, Devlin HB, Wright GG, Maine RJ, Manning MC. Immunizing activity of alum-precipitated protective antigen of Bacillus anthracis in cattle, sheep, and swine. Am J Vet Res 1956;17(63):256–61.
    pubmed: 13302649
  62. Redmond C, Hall GA, Turnbull PCB, Gillgan JS. Experimentally assessed public health risks associated with pigs from farms experiencing anthrax. Vet Record 1997;141(10):244–7.
    pubmed: 9308148doi: 10.1136/vr.141.10.244google scholar: lookup
  63. Van-Ert MN, Easterday WR, Huynh LY, Okinaka RT, Hugh-Jones ME, Ravel J. Global genetic population structure of Bacillus anthracis. PLoS One 2007;2(5):e461.
  64. Hugh-Jones ME. Overview of anthrax, 2015. [February 9; 2021]. Available via https://www.msdvetmanual.com/generalized-conditions/anthrax/overview-of-anthrax.
  65. Boyer AE, Quinn CP, Beesley CA, Gallegos-Candela M, Marston CK, Cronin LX. Lethal factor toxemia and anti-protective antigen antibody activity in naturally acquired cutaneous anthrax. J Infect Dis 2011;204(9):1321–7.
    pmc: PMC3182309pubmed: 21908727doi: 10.1093/infdis/jir543google scholar: lookup
  66. Centers for Disease Control and Prevention (CDC). Sentinel level clinical microbiology laboratory guidelines for suspected agents of bioterrorism and emerging infectious diseases: Bacillus anthracis. Atlanta, GA: CDC; 2010. Available via http://www.asm.org/images/pdf/ Clinical/Protocols/anthrax.pdf.
  67. Spencer RC. Bacillus anthracis. J Clin Pathol 2003;56(3):182–7.
    pmc: PMC1769905pubmed: 12610093
  68. Banada PP, Deshpande S, Russo R, Singleton E, Shah D, Patel B. Rapid detection of Bacillus anthracis bloodstream infections by use of a novel assay in the GeneXpert system. J Clin Microbiol 2017;55(10):2964–71.
    pmc: PMC5625382pubmed: 28747367
  69. De BK, Bragg SL, Sanden GN, Wilson KE, Diem LA, Marston CK. Two-component direct fluorescent-antibody assay for rapid identification of Bacillus anthracis. Emerg Infect Dis 2002;8(10):1060–5.
    pmc: PMC2730293pubmed: 12396916doi: 10.3201/eid0810.020392google scholar: lookup
  70. Nazir KHMNH, Hassan J, Chowdhury SMZH, Rahman MB. Novel multiplex-PCR for rapid detection of Bacillus anthracis spores present in soils of Sirajganj district in Bangladesh. Progress Agric 2015;26(1):67–70.
    doi: 10.3329/pa.v26i1.24518google scholar: lookup
  71. Burans J, Keleher A, O’Brien T, Hager J, Plummer A, Morgan C. Rapid method for the diagnosis of Bacillus anthracis infection in clinical samples using a hand-held assay. Salisbury Med Bull 1996;87:36–7.
  72. Muller JD, Wilks CR, O’Riley KJ, Condron RJ, Bull R, Mateczun A. Specificity of an immunochromato‐graphic test for anthrax. Aus Vet J 2004;82(4):220–2.
    pubmed: 15149073
  73. WHO. Manual for laboratory diagnosis of anthrax. New Delhi, India: WHO Regional Office for South-East Asia (SEAHLM- 371); 2003.
  74. WHO. Guidance on regulations for the transport of infectious substances 2007–2008. Geneva, Switzerland: World Health Organization; 2007; pp. 2–26.
  75. Animal Health Diagnostic Center, Cornell University, College of Veterinary Medicine. Anthrax Sample Collection and Shipping Guidelines. [February 22; 2021]. Available via https://www.vet.cornell.edu/animal-health-diagnostic-center/testing/protocols/anthrax.
  76. Samad MA. Animal husbandry and veterinary science. 1st. Mymensingh, Bangladesh: Mrs Mahfuza Bulbul Publication; 2008.
  77. Turnbull PJNPc-A. Wildlife-south Africa(02): (Kruger NP): Vultures do not deserve castigation as spreaders of anthrax. Anthrax, Wildlife-South Africa, ProMED South Africa: International Society for Infectious Disease, Krueger National Park; 2010.
  78. Himsworth CG. The danger of lime use in agricultural anthrax disinfection procedures: the potential role of calcium in the preservation of anthrax spores. Canadian Vet J 2008;49(12):1208.
    pmc: PMC2583417pubmed: 19252713
  79. Sterne M. Variation in Bacillus anthracis. Onderstepoort J Vet Sci Anim Indus 1937;8:271–349.
  80. Sterne M. The effects of different carbon dioxide concentrations on the growth of virulent anthrax strains. Pathogenicity and immunity tests on guineapigs and sheep with anthrax variants derived from virulent strains. Onderstepoort J Vet Sci Anim Indus 1937;9:49–67.
  81. Alper T. Development of the Sterne strain of anthrax. Salisbury Med Bull 1996;87S:100–1.
  82. Sarker MSA, El Zowalaty ME, Shahid MAH, Sarker MA, Rahman MB, Järhult JD. Maximization of livestock anthrax vaccination coverage in bangladesh: An alternative approach. Vaccines 2020;8(3):1–12.
    pmc: PMC7563472pubmed: 32759647doi: 10.3390/vaccines8030435google scholar: lookup
  83. Aryal S. Ring precipitation test—objectives, principle, procedure, results and examples. Microbe Notes 2018. [5 May 2021]. Available via https://microbenotes.com/ring-precipitation-test-objectives-principle-procedure-results-and-examples/.