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Rotation of liquid-preserved artificial insemination doses on roller benches affects sperm quality during storage in stallions.
Abstract: Appropriate stallion semen handling is of great importance in equine artificial insemination (AI) industry. Optimal treatment of AI doses is aiming for best sperm preservation by excluding strong environmental influences and adding media that favour sperm survival. One method widely used in stallion sperm handling is the rotation of liquid-preserved semen samples on roller benches during storage. As previous studies in boars give rise to the fact that rotation should not be considered beneficial for spermatozoa anymore, the present study investigated the influence of continuous rotation of diluted stallion AI portions on sperm quality. Ejaculates (n = 15) were collected at a German AI centre and diluted with the two extenders EquiPlus and Gent (Minitüb GmbH) to a final concentration of 50 × 10 sperm/mL. Afterwards, samples were placed separately on roller benches at 5°C in the dark, obtaining a rotation frequency of 5 revolutions per minute (rpm) and 36 rpm for four consecutive days following a split-sample design. Both groups were analysed daily in comparison to a control group (0 rpm) with an extended spectrum of spermatological methods including computer-assisted sperm analysis and flow cytometry. Statistical analyses were based on the calculation of generalized linear mixed models for each sperm parameter. The research revealed a decrease in sperm quality parameters of rotated samples compared to non-rotated control groups, visible in total sperm motility (p < .001), decreased thermo-resistance (p < .01) and a drop in pH (p .05) were detected between rotation frequencies of 5 and 36 rpm. We conclude that the fertilizing capacity of stallion semen was negatively affected by rotation during storage in vitro, irrespective of the rotation frequency. Further studies need to investigate whether field fertility in horses is similarly affected by semen rotation on roller benches in vivo.
© 2023 Wiley-VCH GmbH. Published by John Wiley & Sons Ltd.
Publication Date: 2023-08-19 PubMed ID: 37596825DOI: 10.1111/rda.14456Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
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The research article is focused on studying how the rotation of liquid-preserved stallion sperm samples on roller benches during storage affects sperm quality, and finds that continuous rotation diminishes sperm quality parameters irrespective of the rotation frequency.
Objective of the Research
- The research purports to understand the impact of the usual method of sperm handling in stallions, specifically the continuous rotation of liquid-preserved semen samples on roller benches during storage, on the quality of sperm.
- The research also tries to ascertain whether the frequency of rotation changes the impact on sperm quality.
Research Methodology
- The study was conducted using 15 ejaculates collected at a German AI (Artificial Insemination) centre, diluted to a specific concentration.
- They were separately placed on roller benches at a particular temperature in dark, achieving rotation frequencies of 5 revolutions per minute (rpm) and 36 rpm for four straight days.
- These two groups were then compared with a control group (0 rpm) on a daily basis using advanced spermatological methods including computer-assisted sperm analysis and flow cytometry.
- The statistical analysis involved the calculation of generalized linear mixed models for each sperm parameter.
Research Findings
- The result of the research indicated a drop in sperm quality parameters when the samples were continuously rotated, seen as decrease in total sperm motility, decreased thermo-resistance, and a drop in pH.
- Interestingly, it was detected that there were no marked differences in the results between 5 rpm and 36 rpm rotations.
- The conclusion was that irrespective of the rotation frequency, rotation during storage in vitro negatively affects the fertilizing capacity of stallion semen, thereby implying that the widely prevalent rotation method of sperm handling in stallions might not be conducive to the aim of best sperm preservation.
Further Research Direction
- This significant finding raises an important question for further research: whether the field fertility in horses is similarly affected by semen rotation on roller benches, but this time in vivo.
Cite This Article
APA
Dierberger H, Pieper L, Jung M, Schulze M.
(2023).
Rotation of liquid-preserved artificial insemination doses on roller benches affects sperm quality during storage in stallions.
Reprod Domest Anim.
https://doi.org/10.1111/rda.14456 Publication
Researcher Affiliations
- Institute for Reproduction of Farm Animals Schu00f6now, Bernau, Germany.
- Institute for Reproduction of Farm Animals Schu00f6now, Bernau, Germany.
- Institute for Reproduction of Farm Animals Schu00f6now, Bernau, Germany.
- Institute for Reproduction of Farm Animals Schu00f6now, Bernau, Germany.
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