Analyze Diet
EFSA journal. European Food Safety Authority2020; 18(6); e06146; doi: 10.2903/j.efsa.2020.6146

Safety and efficacy of turmeric extract, turmeric oil, turmeric oleoresin and turmeric tincture from Curcuma longa L. rhizome when used as sensory additives in feed for all animal species.

Abstract: Following a request from the European Commission, the EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) was asked to deliver a scientific opinion on the safety and efficacy of turmeric extract, turmeric oil, turmeric oleoresin and turmeric tincture from L. rhizome when used as sensory additives in feed and in water for drinking for all animal species. The FEEDAP Panel concludes that the additives under consideration are safe at the maximum proposed use levels: (i) turmeric extract at 15 mg/kg complete feed (or in water for drinking at comparable exposure) for all animal species; (ii) turmeric essential oil at 80 mg/kg feed for veal calves (milk replacer) and 20 mg/kg complete feed (or 20 mg/L) for all other species; (iii) turmeric oleoresin at 30 mg/kg complete feed (or 30 mg/L) for chickens for fattening and laying hens and 5 mg/kg complete feed (or 5 mg/L) for pigs, veal calves, cattle for fattening and dairy cows, sheep, goats, horses, rabbits and fish; (iv) turmeric tincture at 0.8 mL/L water for drinking for poultry, 6 mL per head and day for horses and 0.05 mL tincture/kg complete feed for dogs. No concerns for consumers were identified following the use of the additives at the proposed use level in animal nutrition. Turmeric extract, turmeric oil, turmeric oleoresin and turmeric tincture should be considered as irritants to skin and eyes and the respiratory tract and as skin sensitisers. The use of the additives in feed is not expected to pose a risk for the environment. Since turmeric and its preparations are recognised to flavour food and their function in feed would be essentially the same as that in food, no further demonstration of efficacy is considered necessary.
Publication Date: 2020-06-12 PubMed ID: 32874324PubMed Central: PMC7448085DOI: 10.2903/j.efsa.2020.6146Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research paper mainly discusses the safety and effectiveness of various forms of turmeric (including extract, oil, oleoresin, and tincture) derived from Curcuma longa L. rhizome when used as sensory additives in feed and drinking water for different animal species. The study was carried out by the EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) following a request from the European Commission.

Summary of Key Findings

According to the analysis by FEEDAP Panel, these turmeric-based additives were found to be safe when used within specified limits for different animals, and there were no identified risks for consumers of products derived from animals consuming these additives. The additives were, however, noted as potential irritants to skin, eyes, and the respiratory tract. No significant environmental risks were identified.

  • The maximum safe use level for turmeric extract was established at 15 mg/kg complete feed (or an equivalent level in drinking water) for all animal species.
  • For turmeric essential oil, the highest safe use level was 80 mg/kg feed for veal calves and 20 mg/kg full feed (or 20 mg/L) for all other species.
  • With turmeric oleoresin, safe use levels were marked at 30 mg/kg complete feed (or 30 mg/L) for fattening chickens and laying hens, and 5 mg/kg full feed (or 5 mg/L) for other animals like pigs, veal calves, cattle for fattening and dairy cows, sheep, goats, horses, rabbits, and fish.
  • For turmeric tincture, the deemed levels were 0.8 mL/L water for poultry, 6 mL per head and day for horses, and 0.05 mL tincture/kg full feed for dogs.

Safety Concerns and Implications

None of these turmeric derivatives posed any concerns for consumers. These findings allow for the safe inclusion of turmeric additives in animal nutrition at the proposed use level. However, these additives can be a skin irritant and a sensitizer, and can also cause eye irritation and affect the respiratory tract. Therefore, necessary precautions should be taken while handling these additives.

Environmental Impact and Efficacy

The study concluded that the use of these additives in animal feed is not expected to cause any environmental harm. As for the efficacy, turmeric and its preparations are recognised to flavour food, and their function in feed would be essentially the same as that in food, rendering it unnecessary for a further demonstration of efficacy.

Cite This Article

APA
Bampidis V, Azimonti G, Bastos ML, Christensen H, Kos Durjava M, Kouba M, López-Alonso M, López Puente S, Marcon F, Mayo B, Pechová A, Petkova M, Ramos F, Sanz Y, Villa RE, Woutersen R, Brantom P, Chesson A, Westendorf J, Gregoretti L, Manini P, Dusemund B. (2020). Safety and efficacy of turmeric extract, turmeric oil, turmeric oleoresin and turmeric tincture from Curcuma longa L. rhizome when used as sensory additives in feed for all animal species. EFSA J, 18(6), e06146. https://doi.org/10.2903/j.efsa.2020.6146

Publication

ISSN: 1831-4732
NlmUniqueID: 101642076
Country: United States
Language: English
Volume: 18
Issue: 6
Pages: e06146
PII: e06146

Researcher Affiliations

Bampidis, Vasileios
    Azimonti, Giovanna
      Bastos, Maria de Lourdes
        Christensen, Henrik
          Kos Durjava, Mojca
            Kouba, Maryline
              López-Alonso, Marta
                López Puente, Secundino
                  Marcon, Francesca
                    Mayo, Baltasar
                      Pechová, Alena
                        Petkova, Mariana
                          Ramos, Fernando
                            Sanz, Yolanda
                              Villa, Roberto Edoardo
                                Woutersen, Ruud
                                  Brantom, Paul
                                    Chesson, Andrew
                                      Westendorf, Johannes
                                        Gregoretti, Lucilla
                                          Manini, Paola
                                            Dusemund, Birgit

                                              References

                                              This article includes 110 references
                                              1. Aggarwal ML, Chacko KM, Kuruvilla BT. Systematic and comprehensive investigation of the toxicity of curcuminoid‑essential oil complex: A bioavailable turmeric formulation.. Mol Med Rep 2016 Jan;13(1):592-604.
                                                pmc: PMC4686098pubmed: 26648561doi: 10.3892/mmr.2015.4579google scholar: lookup
                                              2. Antunes LM, Araújo MC, Dias FL, Takahashi CS. Modulatory effects of curcumin on the chromosomal damage induced by doxorubicin in Chinese hamster ovary cells.. Teratog Carcinog Mutagen 1999;19(1):1-8.
                                              3. Asai A, Miyazawa T. Occurrence of orally administered curcuminoid as glucuronide and glucuronide/sulfate conjugates in rat plasma.. Life Sci 2000 Oct 27;67(23):2785-93.
                                                pubmed: 11105995doi: 10.1016/s0024-3205(00)00868-7google scholar: lookup
                                              4. Au W, Hsu TC. Studies on the clastogenic effects of biologic stains and dyes.. Environ Mutagen 1979;1(1):27-35.
                                                pubmed: 95447doi: 10.1002/em.2860010109google scholar: lookup
                                              5. Avci H, Sekkin S, Boyacioğlu M, Akşit H, Tunca R, Epikmen ET, Birincioğlu SS. Ratlarda deneysel siklofosfamid toksikasyonunda silimarin ve curcuminin koruyucu ve antigenotoksik etkileri. Kafkas Univ Vet Fak Derg .
                                                doi: 10.9775/kvfd.2016.15145google scholar: lookup
                                              6. Awasthi PK, Dixit SC. Chemical composition of Curcuma longa leaves and Rhizomes Oil form the Plains of Northern India. Journal of Young Pharmacists 2009;1:312–316.
                                              7. Al Ayoub Y, Gopalan RC, Najafzadeh M, Mohammad MA, Anderson D, Paradkar A, Assi KH. Development and evaluation of nanoemulsion and microsuspension formulations of curcuminoids for lung delivery with a novel approach to understanding the aerosol performance of nanoparticles.. Int J Pharm 2019 Feb 25;557:254-263.
                                                pubmed: 30597263doi: 10.1016/j.ijpharm.2018.12.042google scholar: lookup
                                              8. Banerjee A, Kunwar A, Mishra B, Priyadarsini KI. Concentration dependent antioxidant/pro-oxidant activity of curcumin studies from AAPH induced hemolysis of RBCs.. Chem Biol Interact 2008 Jul 30;174(2):134-9.
                                                pubmed: 18571152doi: 10.1016/j.cbi.2008.05.009google scholar: lookup
                                              9. Blasiak J, Trzeciak A, Kowalik J. Curcumin damages DNA in human gastric mucosa cells and lymphocytes.. J Environ Pathol Toxicol Oncol 1999;18(4):271-6.
                                                pubmed: 15281237
                                              10. Błasiak J, Trzeciak A, Małecka-Panas E, Drzewoski J, Iwanienko T, Szumiel I, Wojewódzka M. DNA damage and repair in human lymphocytes and gastric mucosa cells exposed to chromium and curcumin.. Teratog Carcinog Mutagen 1999;19(1):19-31.
                                              11. Bojko A, Cierniak A, Adamczyk A, Ligeza J. Modulatory Effects of Curcumin and Tyrphostins (AG494 and AG1478) on Growth Regulation and Viability of LN229 Human Brain Cancer Cells.. Nutr Cancer 2015;67(7):1170-82.
                                                pubmed: 26364505doi: 10.1080/01635581.2015.1073764google scholar: lookup
                                              12. Burdock GA. Fenaroli's handbook of flavor ingredients, 6th Edition. CRC Press. Taylor & Francis Group, Boca Raton, FL 2010:pp. 276–277.
                                              13. Burgos-Morón E, Calderón-Montaño JM, Salvador J, Robles A, López-Lázaro M. The dark side of curcumin.. Int J Cancer 2010 Apr 1;126(7):1771-5.
                                                pubmed: 19830693doi: 10.1002/ijc.24967google scholar: lookup
                                              14. Cao J, Jia L, Zhou HM, Liu Y, Zhong LF. Mitochondrial and nuclear DNA damage induced by curcumin in human hepatoma G2 cells.. Toxicol Sci 2006 Jun;91(2):476-83.
                                                pubmed: 16537656doi: 10.1093/toxsci/kfj153google scholar: lookup
                                              15. Cao J, Jiang LP, Liu Y, Yang G, Yao XF, Zhong LF. Curcumin-induced genotoxicity and antigenotoxicity in HepG2 cells.. Toxicon 2007 Jun 15;49(8):1219-22.
                                                pubmed: 17433396doi: 10.1016/j.toxicon.2007.02.006google scholar: lookup
                                              16. Çelik A, Eke D, Ekinci SY, Yıldırım S. The protective role of curcumin on perfluorooctane sulfonate-induced genotoxicity: single cell gel electrophoresis and micronucleus test.. Food Chem Toxicol 2013 Mar;53:249-55.
                                                pubmed: 23246701doi: 10.1016/j.fct.2012.11.054google scholar: lookup
                                              17. CoE (Council of Europe). Natural sources of flavourings. Report No. 1. Curcuma longa L.. Council of Europe Publishing 2000:pp. 161–162.
                                              18. Court MH, Greenblatt DJ. Molecular basis for deficient acetaminophen glucuronidation in cats. An interspecies comparison of enzyme kinetics in liver microsomes.. Biochem Pharmacol 1997 Apr 4;53(7):1041-7.
                                                pubmed: 9174118doi: 10.1016/s0006-2952(97)00072-5google scholar: lookup
                                              19. Damarla SR, Komma R, Bhatnagar U, Rajesh N, Mulla SMA. An Evaluation of the Genotoxicity and Subchronic Oral Toxicity of Synthetic Curcumin.. J Toxicol 2018;2018:6872753.
                                                doi: 10.1155/2018/6872753pmc: PMC6077508pubmed: 30111997google scholar: lookup
                                              20. Dandekar P, Dhumal R, Jain R, Tiwari D, Vanage G, Patravale V. Toxicological evaluation of pH-sensitive nanoparticles of curcumin: acute, sub-acute and genotoxicity studies.. Food Chem Toxicol 2010 Aug-Sep;48(8-9):2073-89.
                                                pubmed: 20470854doi: 10.1016/j.fct.2010.05.008google scholar: lookup
                                              21. Dandekar PP, Jain R, Patil S, Dhumal R, Tiwari D, Sharma S, Vanage G, Patravale V. Curcumin-loaded hydrogel nanoparticles: application in anti-malarial therapy and toxicological evaluation.. J Pharm Sci 2010 Dec;99(12):4992-5010.
                                                pubmed: 20821383doi: 10.1002/jps.22191google scholar: lookup
                                              22. EFSA (European Food Safety Authority). Technical Guidance of the Scientific Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) for assessing the safety of feed additives for the environment. EFSA Journal 2008;6(10):842, 28 pp.
                                                doi: 10.2903/j.efsa.2008.842google scholar: lookup
                                              23. EFSA (European Food Safety Authority). Compendium of botanicals reported to contain naturally occurring substances of possible concern for human health when used in food and food supplements. EFSA Journal 2012;10(5):2663, 60 pp.
                                                doi: 10.2903/j.efsa.2012.2663google scholar: lookup
                                              24. EFSA (European Food Safety Authority). Refined exposure assessment for curcumin (E 100). EFSA Journal 2014;12(10):3876, 43 pp.
                                                doi: 10.2903/j.efsa.2014.3876google scholar: lookup
                                              25. EFSA ANS Panel (EFSA Panel on Food Additives and Nutrient Sources added to Food). Scientific Opinion on the re‐evaluation of curcumin (E 100) as a food additive. EFSA Journal 2010;8(9):1679, 46 pp.
                                                doi: 10.2903/j.efsa.2010.1679google scholar: lookup
                                              26. EFSA FEEDAP Panel (EFSA Panel on Additives and Products or Substances used in Animal Feed). Statement on the use of feed additives authorised/applied for use in feed when supplied via water. EFSA Journal 2010;8(12):1956, 9 pp.
                                                doi: 10.2903/j.efsa.2010.1956google scholar: lookup
                                              27. EFSA FEEDAP Panel (EFSA Panel on Additives and Products or Substances used in Animal Feed). Guidance for the preparation of dossiers for sensory additives. EFSA Journal 2012;10(1):2534, 26 pp.
                                                doi: 10.2903/j.efsa.2012.2534google scholar: lookup
                                              28. EFSA FEEDAP Panel (EFSA Panel on Additives and Products or Substances used in Animal Feed). Guidance for the preparation of dossiers for additives already authorised for use in food. EFSA Journal 2012;10(1):2538, 4 pp.
                                                doi: 10.2903/j.efsa.2012.2538google scholar: lookup
                                              29. EFSA FEEDAP Panel (EFSA Panel on Additives and Products or Substances used in Animal Feed). Guidance on studies concerning the safety of use of the additive for users/workers. EFSA Journal 2012;10(1):2539, 5 pp.
                                                doi: 10.2903/j.efsa.2012.2539google scholar: lookup
                                              30. EFSA FEEDAP Panel (EFSA Panel on Additives and Products or Substances used in Animal Feed). Scientific opinion on the safety and efficacy of aliphatic, alicyclic and aromatic saturated and unsaturated tertiary alcohols and esters with esters containing tertiary alcohols ethers (chemical group 6) when used as flavourings for all animal species. EFSA Journal 2012;10(11):2966, 25 pp.
                                                doi: 10.2903/j.efsa.2012.2966google scholar: lookup
                                              31. EFSA FEEDAP Panel (EFSA Panel on Additives and Products or Substances used in Animal Feed). Scientific Opinion on the safety and efficacy of aliphatic and alicyclic ethers (chemical group 16) when used as flavourings for all animal species. EFSA Journal 2012;10(11):2967, 17 pp.
                                                doi: 10.2903/j.efsa.2012.2967google scholar: lookup
                                              32. EFSA FEEDAP Panel (EFSA Panel on Additives and Products or Substances used in Animal Feed). Scientific Opinion on the safety and efficacy of aliphatic and aromatic hydrocarbons (chemical group 31) when used as flavourings for all animal species. EFSA Journal 2015;13(3):4053, 22 pp.
                                                doi: 10.2903/j.efsa.2015.4053google scholar: lookup
                                              33. Bampidis V, Azimonti G, Bastos ML, Christensen H, Kouba M, Kos Durjava M, López-Alonso M, López Puente S, Marcon F, Mayo B, Pechová A, Petkova M, Ramos F, Sanz Y, Villa RE, Woutersen R, Brantom P, Chesson A, Westendorf J, Gregoretti L, Manini P, Dusemund B. Safety and efficacy of 26 compounds belonging to chemical group 3 (α,β-unsaturated straight-chain and branched-chain aliphatic primary alcohols, aldehydes, acids and esters) when used as flavourings for all animal species and categories.. EFSA J 2019 Mar;17(3):e05654.
                                                doi: 10.2903/j.efsa.2016.4339pmc: PMC7009239pubmed: 32626273google scholar: lookup
                                              34. Rychen G, Aquilina G, Azimonti G, Bampidis V, Bastos ML, Bories G, Chesson A, Cocconcelli PS, Flachowsky G, Gropp J, Kolar B, Kouba M, López-Alonso M, López Puente S, Mantovani A, Mayo B, Ramos F, Saarela M, Villa RE, Wallace RJ, Wester P, Anguita M, Galobart J, Innocenti ML. Guidance on the identity, characterisation and conditions of use of feed additives.. EFSA J 2017 Oct;15(10):e05023.
                                                doi: 10.2903/j.efsa.2017.5023pmc: PMC7010039pubmed: 32625313google scholar: lookup
                                              35. Rychen G, Aquilina G, Azimonti G, Bampidis V, Bastos ML, Bories G, Chesson A, Cocconcelli PS, Flachowsky G, Gropp J, Kolar B, Kouba M, López-Alonso M, López Puente S, Mantovani A, Mayo B, Ramos F, Saarela M, Villa RE, Wallace RJ, Wester P, Anguita M, Galobart J, Innocenti ML, Martino L. Guidance on the assessment of the safety of feed additives for the target species.. EFSA J 2017 Oct;15(10):e05021.
                                                doi: 10.2903/j.efsa.2017.5021pmc: PMC7009839pubmed: 32625311google scholar: lookup
                                              36. Rychen G, Aquilina G, Azimonti G, Bampidis V, Bastos ML, Bories G, Chesson A, Cocconcelli PS, Flachowsky G, Gropp J, Kolar B, Kouba M, López-Alonso M, López Puente S, Mantovani A, Mayo B, Ramos F, Saarela M, Villa RE, Wallace RJ, Wester P, Anguita M, Dujardin B, Galobart J, Innocenti ML. Guidance on the assessment of the safety of feed additives for the consumer.. EFSA J 2017 Oct;15(10):e05022.
                                                doi: 10.2903/j.efsa.2017.5022pmc: PMC7009902pubmed: 32625312google scholar: lookup
                                              37. EFSA Scientific Committee. Guidance on safety assessment of botanicals and botanical preparations intended for use as ingredients in food supplements, on request of EFSA. EFSA Journal 2009;7(9):1249, 19 pp.
                                                doi: 10.2093/j.efsa.2009.1249google scholar: lookup
                                              38. EFSA Scientific Committee. Scientific Opinion on genotoxicity testing strategies applicable to food and feed safety assessment. EFSA Journal 2011;9(9):2379, 69 pp.
                                                doi: 10.2903/j.efsa.2011.2379google scholar: lookup
                                              39. Hardy A, Benford D, Halldorsson T, Jeger MJ, Knutsen HK, More S, Naegeli H, Noteborn H, Ockleford C, Ricci A, Rychen G, Schlatter JR, Silano V, Solecki R, Turck D, Benfenati E, Chaudhry QM, Craig P, Frampton G, Greiner M, Hart A, Hogstrand C, Lambre C, Luttik R, Makowski D, Siani A, Wahlstroem H, Aguilera J, Dorne JL, Fernandez Dumont A, Hempen M, Valtueña Martínez S, Martino L, Smeraldi C, Terron A, Georgiadis N, Younes M. Guidance on the use of the weight of evidence approach in scientific assessments.. EFSA J 2017 Aug;15(8):e04971.
                                                doi: 10.2903/j.efsa.2017.4971pmc: PMC7009893pubmed: 32625632google scholar: lookup
                                              40. More SJ, Bampidis V, Benford D, Bennekou SH, Bragard C, Halldorsson TI, Hernández-Jerez AF, Koutsoumanis K, Naegeli H, Schlatter JR, Silano V, Nielsen SS, Schrenk D, Turck D, Younes M, Benfenati E, Castle L, Cedergreen N, Hardy A, Laskowski R, Leblanc JC, Kortenkamp A, Ragas A, Posthuma L, Svendsen C, Solecki R, Testai E, Dujardin B, Kass GE, Manini P, Jeddi MZ, Dorne JC, Hogstrand C. Guidance on harmonised methodologies for human health, animal health and ecological risk assessment of combined exposure to multiple chemicals.. EFSA J 2019 Mar;17(3):e05634.
                                                doi: 10.2903/j.efsa.2019.5634pmc: PMC7009070pubmed: 32626259google scholar: lookup
                                              41. More S, Bampidis V, Benford D, Boesten J, Bragard C, Halldorsson T, Hernandez-Jerez A, Hougaard-Bennekou S, Koutsoumanis K, Naegeli H, Nielsen SS, Schrenk D, Silano V, Turck D, Younes M, Aquilina G, Crebelli R, Gürtler R, Hirsch-Ernst KI, Mosesso P, Nielsen E, Solecki R, Carfì M, Martino C, Maurici D, Parra Morte J, Schlatter J. Genotoxicity assessment of chemical mixtures.. EFSA J 2019 Jan;17(1):e05519.
                                                doi: 10.2903/j.efsa.2019.5519pmc: PMC7009058pubmed: 32626066google scholar: lookup
                                              42. El‐Makawy A, Sharaf HA. Cytogenetical and histochemical studies on curcumin in male rats. WIT Transactions on Biomedicine and Health 2006;10:169–180.
                                              43. nEMA (European Medicines Agency). Herbal Monograph on Curcuma longa L. rhizoma. European Medicines Agency 2018.
                                              44. nEMA (European Medicines Agency). Assessment report on Curcuma longa L. rhizoma. European Medicines Agency 2018.
                                              45. nFAO (World Health Organization). Curcumin, Chemical and Technical Assessment (CTA). World Health Organization 2004.
                                              46. Farag MR, Alagawany MM, Dhama K. Antidotal effect of turmeric (Curcuma longa) against endosulfan‐induced cytogenotoxicity and immunotoxicity in broiler chicks. International Journal of Pharmacology 2014;10:429–439.
                                              47. Farhadi M, Bakhshandeh M, Shafiei B, Mahmoudzadeh A, Hosseinimehr SJ. The radioprotective effects of nano‐curcumin against genotoxicity induced by iodine‐131 in patients with differentiated thyroid carcinoma (DTC) by micronucleus assay. International Journal of Cancer Management 2018;11:e14193.
                                              48. Galli GM, Da Silva AS, Biazus AH, Reis JH, Boiago MM, Topazio JP, Migliorini MJ, Guarda NS, Moresco RN, Ourique AF, Santos CG, Lopes LS, Baldissera MD, Stefani LM. Feed addition of curcumin to laying hens showed anticoccidial effect, and improved egg quality and animal health.. Res Vet Sci 2018 Jun;118:101-106.
                                                pubmed: 29421478doi: 10.1016/j.rvsc.2018.01.022google scholar: lookup
                                              49. Giri AK, Das SK, Talukder G, Sharma A. Sister chromatid exchange and chromosome aberrations induced by curcumin and tartrazine on mammalian cells in vivo.. Cytobios 1990;62(249):111-7.
                                                pubmed: 2209081
                                              50. Gopi S, Jacob J, Mathur KY. Acute and subchronic oral toxicity studies of hydrogenated curcuminoid formulation 'CuroWhite' in rats.. Toxicol Rep 2016;3:817-825.
                                              51. Gordon ON, Luis PB, Ashley RE, Osheroff N, Schneider C. Oxidative Transformation of Demethoxy- and Bisdemethoxycurcumin: Products, Mechanism of Formation, and Poisoning of Human Topoisomerase IIα.. Chem Res Toxicol 2015 May 18;28(5):989-96.
                                              52. Hassan FU, Rehman MS, Khan MS, Ali MA, Javed A, Nawaz A, Yang C. Curcumin as an Alternative Epigenetic Modulator: Mechanism of Action and Potential Effects.. Front Genet 2019;10:514.
                                                pmc: PMC6557992pubmed: 31214247doi: 10.3389/fgene.2019.00514google scholar: lookup
                                              53. Haverić A, Haverić S, Hadžić M, Lojo-Kadrić N, Ibrulj S. Genotoxicity and cytotoxicity analysis of curcumin and sunset yellow in human lymphocyte culture.. Cell Mol Biol (Noisy-le-grand) 2018 Feb 28;64(3):87-91.
                                                pubmed: 29506635doi: 10.14715/cmb/2018.64.3.14google scholar: lookup
                                              54. Heger M, van Golen RF, Broekgaarden M, Michel MC. The molecular basis for the pharmacokinetics and pharmacodynamics of curcumin and its metabolites in relation to cancer.. Pharmacol Rev 2014;66(1):222-307.
                                                pubmed: 24368738doi: 10.1124/pr.110.004044google scholar: lookup
                                              55. Her C, Venier‐Julienne MC, Roger E. Improvement of curcumin bioavailability for medical applications. Medicinal and Aromatic Plants 2018;7:6.
                                              56. Ireson C, Orr S, Jones DJ, Verschoyle R, Lim CK, Luo JL, Howells L, Plummer S, Jukes R, Williams M, Steward WP, Gescher A. Characterization of metabolites of the chemopreventive agent curcumin in human and rat hepatocytes and in the rat in vivo, and evaluation of their ability to inhibit phorbol ester-induced prostaglandin E2 production.. Cancer Res 2001 Feb 1;61(3):1058-64.
                                                pubmed: 11221833
                                              57. Jäger R, Lowery RP, Calvanese AV, Joy JM, Purpura M, Wilson JM. Comparative absorption of curcumin formulations.. Nutr J 2014 Jan 24;13:11.
                                                pmc: PMC3918227pubmed: 24461029doi: 10.1186/1475-2891-13-11google scholar: lookup
                                              58. Jain AK, Tezuka H, Kada T, Tomita I. Evaluation of genotoxic effects of turmeric in mice. Current Science. Bangalore 1987;56:1005–1006.
                                              59. Jensen NJ. Lack of mutagenic effect of turmeric oleoresin and curcumin in the Salmonella/mammalian microsome test.. Mutat Res 1982 Dec;105(6):393-6.
                                                pubmed: 6759931doi: 10.1016/0165-7992(82)90182-8google scholar: lookup
                                              60. Ketron AC, Gordon ON, Schneider C, Osheroff N. Oxidative metabolites of curcumin poison human type II topoisomerases.. Biochemistry 2013 Jan 8;52(1):221-7.
                                                pmc: PMC3541001pubmed: 23253398doi: 10.1021/bi3014455google scholar: lookup
                                              61. Khatik R, Dwivedi P, Shukla A, Srivastava P, Rath SK, Paliwal SK, Dwivedi AK. Development, characterization and toxicological evaluations of phospholipids complexes of curcumin for effective drug delivery in cancer chemotherapy.. Drug Deliv 2016;23(3):1067-78.
                                                pubmed: 25033042doi: 10.3109/10717544.2014.936988google scholar: lookup
                                              62. Kocyigit A, Guler EM. Curcumin induce DNA damage and apoptosis through generation of reactive oxygen species and reducing mitochondrial membrane potential in melanoma cancer cells.. Cell Mol Biol (Noisy-le-grand) 2017 Nov 30;63(11):97-105.
                                                pubmed: 29208180doi: 10.14715/cmb/2017.63.11.17google scholar: lookup
                                              63. Lewinska A, Wnuk M, Grabowska W, Zabek T, Semik E, Sikora E, Bielak-Zmijewska A. Curcumin induces oxidation-dependent cell cycle arrest mediated by SIRT7 inhibition of rDNA transcription in human aortic smooth muscle cells.. Toxicol Lett 2015 Mar 18;233(3):227-38.
                                                pubmed: 25644192doi: 10.1016/j.toxlet.2015.01.019google scholar: lookup
                                              64. Li S, Yuan W, Deng G, Wang P, Yang P, Aggarwal BB. Chemical composition and product quality control of turmeric (Curcuma longa L,). Pharmaceutical Crops 2011;2:28–54.
                                              65. Liju VB, Jeena K, Kuttan R. Acute and subchronic toxicity as well as mutagenic evaluation of essential oil from turmeric (Curcuma longa L).. Food Chem Toxicol 2013 Mar;53:52-61.
                                                pubmed: 23201370doi: 10.1016/j.fct.2012.11.027google scholar: lookup
                                              66. Liju VB, Jeena K, Kumar D, Maliakel B, Kuttan R, I M K. Enhanced bioavailability and safety of curcumagalactomannosides as a dietary ingredient.. Food Funct 2015 Jan;6(1):276-86.
                                                pubmed: 25428277doi: 10.1039/c4fo00749bgoogle scholar: lookup
                                              67. Liu A, Lou H, Zhao L, Fan P. Validated LC/MS/MS assay for curcumin and tetrahydrocurcumin in rat plasma and application to pharmacokinetic study of phospholipid complex of curcumin.. J Pharm Biomed Anal 2006 Feb 24;40(3):720-7.
                                                pubmed: 16316738doi: 10.1016/j.jpba.2005.09.032google scholar: lookup
                                              68. Marczylo TH, Steward WP, Gescher AJ. Rapid analysis of curcumin and curcumin metabolites in rat biomatrices using a novel ultraperformance liquid chromatography (UPLC) method.. J Agric Food Chem 2009 Feb 11;57(3):797-803.
                                                pubmed: 19152267doi: 10.1021/jf803038fgoogle scholar: lookup
                                              69. Mehrotra N, Sabarinath S, Suryawanshi S, Raj K, Gupta RC. LC–UV assay for simultaneous estimation of aromatic turmerone, α/β‐turmerone and curlone: major bisabolane sesquiterpenes of turmeric oil in rabbit plasma for application to pharmacokinetic studies. Chromatographia 2009;69:1077–1082.
                                              70. Mendonça LM, Dos Santos GC, Antonucci GA, Dos Santos AC, Bianchi Mde L, Antunes LM. Evaluation of the cytotoxicity and genotoxicity of curcumin in PC12 cells.. Mutat Res 2009 Apr 30;675(1-2):29-34.
                                              71. Mendonça LM, dos Santos GC, dos Santos RA, Takahashi CS, Bianchi Mde L, Antunes LM. Evaluation of curcumin and cisplatin-induced DNA damage in PC12 cells by the alkaline comet assay.. Hum Exp Toxicol 2010 Aug;29(8):635-43.
                                                pubmed: 20051457doi: 10.1177/0960327109358731google scholar: lookup
                                              72. Mendonça LM, Machado Cda S, Teixeira CC, Freitas LA, Bianchi ML, Antunes LM. Comparative study of curcumin and curcumin formulated in a solid dispersion: Evaluation of their antigenotoxic effects.. Genet Mol Biol 2015 Dec;38(4):490-8.
                                              73. Nagabhushan M, Bhide SV. Nonmutagenicity of curcumin and its antimutagenic action versus chili and capsaicin.. Nutr Cancer 1986;8(3):201-10.
                                                pubmed: 3526291doi: 10.1080/01635588609513894google scholar: lookup
                                              74. Nagabhushan M, Bhide SV. Antimutagenicity and anticarcinogenicity of turmeric (Curcuma longa). Journal of Nutrition Growth and Cancer 1987;4:83–89.
                                              75. Nagabhushan M, Amonkar AJ, Bhide SV. In vitro antimutagenicity of curcumin against environmental mutagens.. Food Chem Toxicol 1987 Jul;25(7):545-7.
                                                pubmed: 3623345doi: 10.1016/0278-6915(87)90207-9google scholar: lookup
                                              76. Nair J, Strand S, Frank N, Knauft J, Wesch H, Galle PR, Bartsch H. Apoptosis and age-dependant induction of nuclear and mitochondrial etheno-DNA adducts in Long-Evans Cinnamon (LEC) rats: enhanced DNA damage by dietary curcumin upon copper accumulation.. Carcinogenesis 2005 Jul;26(7):1307-15.
                                                pubmed: 15790590doi: 10.1093/carcin/bgi073google scholar: lookup
                                              77. Nelson KM, Dahlin JL, Bisson J, Graham J, Pauli GF, Walters MA. The Essential Medicinal Chemistry of Curcumin.. J Med Chem 2017 Mar 9;60(5):1620-1637.
                                              78. . NTP Toxicology and Carcinogenesis Studies of Turmeric Oleoresin (CAS No. 8024-37-1) (Major Component 79%-85% Curcumin, CAS No. 458-37-7) in F344/N Rats and B6C3F1 Mice (Feed Studies).. Natl Toxicol Program Tech Rep Ser 1993 Aug;427:1-275.
                                                pubmed: 12616304
                                              79. Papiez MA. The influence of curcumin on the action of etoposide in a rat acute myeloid leukemia cell line.. Folia Med Cracov 2013;53(2):61-72.
                                                pubmed: 24858458
                                              80. Polasa K, Sesikaran B, Krishna TP, Krishnaswamy K. Turmeric (Curcuma longa)-induced reduction in urinary mutagens.. Food Chem Toxicol 1991 Oct;29(10):699-706.
                                                pubmed: 1660015doi: 10.1016/0278-6915(91)90128-tgoogle scholar: lookup
                                              81. Purpura M, Lowery RP, Wilson JM, Mannan H, Münch G, Razmovski-Naumovski V. Analysis of different innovative formulations of curcumin for improved relative oral bioavailability in human subjects.. Eur J Nutr 2018 Apr;57(3):929-938.
                                                pmc: PMC5861163pubmed: 28204880doi: 10.1007/s00394-016-1376-9google scholar: lookup
                                              82. Raina VK, Srivastava SK, Syamsundar KV. Rhizome and leaf oil composition of Curcuma longa from the lower Himalayan Region of Northern India. Journal of Essential Oil Research 2005;17:4 pp.
                                              83. Ravikumar AN, Jacob J, Gopi S, Jagannath TS. A Toxicological Evaluation of a Standardized Hydrogenated Extract of Curcumin (CuroWhite™).. J Toxicol 2018;2018:5243617.
                                                doi: 10.1155/2018/5243617pmc: PMC5828043pubmed: 29610573google scholar: lookup
                                              84. Sakihama Y, Cohen MF, Grace SC, Yamasaki H. Plant phenolic antioxidant and prooxidant activities: phenolics-induced oxidative damage mediated by metals in plants.. Toxicology 2002 Aug 1;177(1):67-80.
                                                pubmed: 12126796doi: 10.1016/s0300-483x(02)00196-8google scholar: lookup
                                              85. Saleh EM, El-awady RA, Eissa NA, Abdel-Rahman WM. Antagonism between curcumin and the topoisomerase II inhibitor etoposide: a study of DNA damage, cell cycle regulation and death pathways.. Cancer Biol Ther 2012 Sep;13(11):1058-71.
                                                pmc: PMC3461813pubmed: 22895066doi: 10.4161/cbt.21078google scholar: lookup
                                              86. Sebastià N, Soriano JM, Barquinero JF, Villaescusa JI, Almonacid M, Cervera J, Such E, Silla MA, Montoro A. In vitro cytogenetic and genotoxic effects of curcumin on human peripheral blood lymphocytes.. Food Chem Toxicol 2012 Sep;50(9):3229-33.
                                                pubmed: 22713711doi: 10.1016/j.fct.2012.06.012google scholar: lookup
                                              87. Shah RG, Netrawali MS. Evaluation of mutagenic activity of turmeric extract containing curcumin, before and after activation with mammalian cecal microbial extract of liver microsomal fraction, in the Ames Salmonella test.. Bull Environ Contam Toxicol 1988 Mar;40(3):350-7.
                                                pubmed: 3285917doi: 10.1007/bf01689091google scholar: lookup
                                              88. Sharma RA, Euden SA, Platton SL, Cooke DN, Shafayat A, Hewitt HR, Marczylo TH, Morgan B, Hemingway D, Plummer SM, Pirmohamed M, Gescher AJ, Steward WP. Phase I clinical trial of oral curcumin: biomarkers of systemic activity and compliance.. Clin Cancer Res 2004 Oct 15;10(20):6847-54.
                                                pubmed: 15501961doi: 10.1158/1078-0432.ccr-04-0744google scholar: lookup
                                              89. Shen L, Ji HF. Bidirectional interactions between dietary curcumin and gut microbiota.. Crit Rev Food Sci Nutr 2019;59(18):2896-2902.
                                                pubmed: 29781709doi: 10.1080/10408398.2018.1478388google scholar: lookup
                                              90. Shen L, Liu CC, An CY, Ji HF. How does curcumin work with poor bioavailability? Clues from experimental and theoretical studies.. Sci Rep 2016 Feb 18;6:20872.
                                                doi: 10.1038/srep20872pmc: PMC4757858pubmed: 26887346google scholar: lookup
                                              91. Sherin S, Sheeja S, Sudha Devi R, Balachandran S, Soumya RS, Abraham A. In vitro and in vivo pharmacokinetics and toxicity evaluation of curcumin incorporated titanium dioxide nanoparticles for biomedical applications.. Chem Biol Interact 2017 Sep 25;275:35-46.
                                                pubmed: 28757137doi: 10.1016/j.cbi.2017.07.022google scholar: lookup
                                              92. Singh S, Panda MK, Subudhi E, Nayak S. Chemical composition of leaf and rhizome oil of an elite genotype Curcuma longa L. from South Eastern Ghats of Orissa. Journal of Pharmacy Research 2010;3:1630–1633.
                                              93. Sivaswamy SN, Balachandran B, Balanehru S, Sivaramakrishnan VM. Mutagenic activity of south Indian food items.. Indian J Exp Biol 1991 Aug;29(8):730-7.
                                                pubmed: 1769715
                                              94. Spalding JW. Genetic toxicity report on turmeric oleoresin (8024–37‐1). 1983.
                                              95. Srividya AR, Dhanbal SP, Sathish Kumar MN, Vishnuvarthan VJ. Comparison of genotoxicity produced by hydro alcoholic extract of Curcuma aromatica salisb, Curcuma zedoaria with curcumin by Ames test, comet assay and micronucleus test. International Research Journal Pharmacology 2013;4:113–119.
                                              96. Tascone O, Roy C, Filippi JJ, Meierhenrich UJ. Use, analysis, and regulation of pesticides in natural extracts, essential oils, concretes, and absolutes.. Anal Bioanal Chem 2014 Feb;406(4):971-80.
                                                pubmed: 23797908doi: 10.1007/s00216-013-7102-zgoogle scholar: lookup
                                              97. Teixeira CCC, Mendonça LM, Bergamaschi MM, Queiroz RHC, Souza GEP, Antunes LMG, Freitas LAP. Microparticles containing curcumin solid dispersion: stability, bioavailability and anti‐inflammatory activity. An Official Journal of the American Association of Pharmaceutical Scientists 2016;17:252–261.
                                              98. Teuscher E. Gewürzdrogen. Wissenschaftliche Verlagsgesellschaft mbH Stuttgart, Ein Handbuch der Gewürze, Gewürzkrauter, Gewürzmischungen und ihrer ätherischen Öle. 2003.
                                              99. Urbina-Cano P, Bobadilla-Morales L, Ramírez-Herrera MA, Corona-Rivera JR, Mendoza-Magaña ML, Troyo-Sanromán R, Corona-Rivera A. DNA damage in mouse lymphocytes exposed to curcumin and copper.. J Appl Genet 2006;47(4):377-82.
                                                pubmed: 17132903doi: 10.1007/bf03194648google scholar: lookup
                                              100. Verma R, Awasthi KK, Rajawat NK, Soni I, John PJ. Curcumin modulates oxidative stress and genotoxicity induced by a type II fluorinated pyrethroid, beta-cyfluthrin.. Food Chem Toxicol 2016 Nov;97:168-176.
                                                pubmed: 27623179doi: 10.1016/j.fct.2016.09.014google scholar: lookup
                                              101. Vijayalaxmi. Genetic effects of turmeric and curcumin in mice and rats.. Mutat Res 1980 Oct;79(2):125-32.
                                                pubmed: 7432370doi: 10.1016/0165-1218(80)90080-4google scholar: lookup
                                              102. nWHO (World Health Organization). Curcumin. WHO Food additives Series (FAS) 1982;17.
                                              103. nWHO (World Health Organization). Curcumin. WHO Food additives Series (FAS) 1996;35.
                                              104. nWHO (World Health Organization). Evaluation of certain food additives and contaminants. Sixty‐first report of the Joint FAO/WHO Expert Committee on Food Additives. WHO Technical Report Series 2004;922.
                                              105. nWHO (World Health Organization). WHO Food additives Series (FAS): 52. 2004.
                                              106. nWHO (World Health Organization). Combined compendium of food additive specifications ‐ all specifications monographs from the 1st to the 65th meeting (1956‐2005). FAO JECFA Monographs Series, No. 1 Volume 1–3 .
                                              107. Yang KY, Lin LC, Tseng TY, Wang SC, Tsai TH. Oral bioavailability of curcumin in rat and the herbal analysis from Curcuma longa by LC-MS/MS.. J Chromatogr B Analyt Technol Biomed Life Sci 2007 Jun 15;853(1-2):183-9.
                                                pubmed: 17400527doi: 10.1016/j.jchromb.2007.03.010google scholar: lookup
                                              108. Yoshino M, Haneda M, Naruse M, Htay HH, Tsubouchi R, Qiao SL, Li WH, Murakami K, Yokochi T. Prooxidant activity of curcumin: copper-dependent formation of 8-hydroxy-2'-deoxyguanosine in DNA and induction of apoptotic cell death.. Toxicol In Vitro 2004 Dec;18(6):783-9.
                                                pubmed: 15465643doi: 10.1016/j.tiv.2004.03.009google scholar: lookup
                                              109. Zheng Y, Chen Y, Jin LW, Ye HY, Liu G. Cytotoxicity and Genotoxicity in Human Embryonic Kidney Cells Exposed to Surface Modify Chitosan Nanoparticles Loaded with Curcumin.. AAPS PharmSciTech 2016 Dec;17(6):1347-1352.
                                                pubmed: 26718819doi: 10.1208/s12249-015-0471-1google scholar: lookup
                                              110. Ziegler H (ed). Turmeric, Curcuma In: Flavourings. Production, Composition, Applications, Regulations. Second completely revised version. Wiley‐VCH Verlag GmbH & Co. KGA, Weinheim 2007:p. 246.

                                              Citations

                                              This article has been cited 2 times.
                                              1. Cattaneo I, Kalian AD, Di Nicola MR, Dujardin B, Levorato S, Mohimont L, Nathanail AV, Carnessechi E, Astuto MC, Tarazona JV, Kass GEN, Liem AKD, Robinson T, Manini P, Hogstrand C, Price PS, Dorne JLCM. Risk Assessment of Combined Exposure to Multiple Chemicals at the European Food Safety Authority: Principles, Guidance Documents, Applications and Future Challenges.. Toxins (Basel) 2023 Jan 4;15(1).
                                                doi: 10.3390/toxins15010040pubmed: 36668860google scholar: lookup
                                              2. Orellana-Paucar AM, Machado-Orellana MG. Pharmacological Profile, Bioactivities, and Safety of Turmeric Oil.. Molecules 2022 Aug 9;27(16).
                                                doi: 10.3390/molecules27165055pubmed: 36014301google scholar: lookup