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Home / Equine Research Bank / J Equine Sci / Sarcoplasmic Reticulum Ca(2+)-ATPase Activity and Glycogen C...
Journal of equine science2009; 20(3); 33-40; doi: 10.1294/jes.20.33

Sarcoplasmic Reticulum Ca(2+)-ATPase Activity and Glycogen Content in Various Fiber Types after Intensive Exercise in Thoroughbred Horses.

Abstract: To find a new parameter indicating muscle fitness in Thoroughbred horses, we examined time-dependent recovery of glycogen content and sarcoplasmic reticulum (SR) Ca(2+)-ATPase activity of skeletal muscle after intensive treadmill running. Two repeated 50-sec running sessions (13 m/sec) were performed on a flat treadmill (approximately 90%VO2max). Muscle samples of the middle gluteal muscle were taken before exercise (pre) and 1 min, 20 min, 60 min, and 24 hr after exercise. Muscle fiber type composition was determined in the pre muscle samples by immunohistochemical staining with monoclonal antibody to myosin heavy chain. SR Ca(2+)-ATPase activity of the muscle and glycogen content of each muscle fiber type were determined with biochemical analysis and quantitative histochemical staining, respectively. As compared to the pre value, the glycogen content of each muscle fiber type was reduced by 15-27% at 1 min, 20 min, and 60 min after the exercise and recovered to the pre value at 24 hr after exercise test. These results indicate that 24 hr is enough time to recover glycogen content after short-term intensive exercise. The mean value of the SR Ca(2+)-ATPase activity showed a slight decrease (not significant) immediately after exercise, and complete recovery at 60 min after exercise. There were no significant relationship between the changes in glycogen content of each muscle fiber type and SR Ca(2+)-ATPase. Although further studies are needed, SR Ca(2+)-ATPase is not a useful parameter to detect muscle fitness, at least in Thoroughbred horses.
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Publication Date: 2009-10-23 PubMed ID: 24833967PubMed Central: PMC4013961DOI: 10.1294/jes.20.33Google Scholar: Lookup
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Summary

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The research investigates the recovery of glycogen content and sarcoplasmic reticulum (SR) Ca(2+)-ATPase activity in different muscle fibers of Thoroughbred horses after intense exercise, aiming to find a means to determine muscle fitness.

Exercise Method and Sample Collection

  • The intensive exercise was conducted using a treadmill, where the horses underwent two bouts of 50-second running sessions at 13 m/sec, which is about 90% of their VO2max, an indicator of aerobic fitness.
  • Samples of the middle gluteal muscle were taken from the horses before the start of the exercise (pre) and at various time points after the exercise — 1 min, 20 min, 60 min, and 24 hr.

Fiber Type and Analysis

  • The muscle fiber type was determined in the pre-exercise samples using immunohistochemical staining, a technique to visualize specific proteins, with a monoclonal antibody against myosin heavy chain.
  • The SR Ca(2+)-ATPase activity of the muscle and the glycogen content of each muscle fiber type were analyzed using a biochemical analysis and quantitative histochemical staining respectively.

Results and Conclusion

  • The glycogen content of each muscle fiber type was found to be reduced by 15-27% within the first hour after exercise but it returned to its pre-exercise level 24 hours after the exercise. This suggests that a full day is sufficient for glycogen recovery after short-term intensive exercise in horses.
  • There was a slight (although not significant) decrease in the mean value of the SR Ca(2+)-ATPase activity immediately after the exercise, which completely recovered 60 minutes after exercising.
  • No significant relationship was observed between the fluctuations in the glycogen content of each muscle fiber type and SR Ca(2+)-ATPase activity.
  • The study concludes that while further investigation is necessary, SR Ca(2+)-ATPase activity does not seem to be a convincing parameter to gauge muscle fitness, at least in Thoroughbred horses.

Cite This Article

APA
Minami Y, Yamano S, Kawai M, Hiraga A, Miyata H. (2009). Sarcoplasmic Reticulum Ca(2+)-ATPase Activity and Glycogen Content in Various Fiber Types after Intensive Exercise in Thoroughbred Horses. J Equine Sci, 20(3), 33-40. https://doi.org/10.1294/jes.20.33

Publication

Journal of equine science
ISSN: 1340-3516
NlmUniqueID: 9503751
Country: Japan
Language: English
Volume: 20
Issue: 3
Pages: 33-40

Researcher Affiliations

Minami, Yoshio
  • Biological Sciences, Graduate School of Medicine, Yamaguchi University, Yoshida 1677-1, Yamaguchi 753-8515, Japan.
Yamano, Seiko
  • Science Research Center, Yamaguchi University, Yoshida 1677-1, Yamaguchi 753-8511, Japan.
Kawai, Minako
  • Biological Sciences, Graduate School of Medicine, Yamaguchi University, Yoshida 1677-1, Yamaguchi 753-8515, Japan.
Hiraga, Atsushi
  • Equine Research Institute, Japan Racing Association, 321-4 Tokami-cho, Utsunomiya, Tochigi 320-0856, Japan.
Miyata, Hirofumi
  • Biological Sciences, Graduate School of Medicine, Yamaguchi University, Yoshida 1677-1, Yamaguchi 753-8515, Japan.

References

This article includes 36 references
  1. Bergström J, Hermansen L, Hultman E, Saltin B. Diet, muscle glycogen and physical performance.. Acta Physiol Scand 1967 Oct-Nov;71(2):140-50.
    pubmed: 5584523doi: 10.1111/j.1748-1716.1967.tb03720.xgoogle scholar: lookup
  2. Byrd SK, McCutcheon LJ, Hodgson DR, Gollnick PD. Altered sarcoplasmic reticulum function after high-intensity exercise.. J Appl Physiol (1985) 1989 Nov;67(5):2072-7.
    pubmed: 2532196doi: 10.1152/jappl.1989.67.5.2072google scholar: lookup
  3. Dossett-Mercer J, Green H, Chin ER, Grange F. Failure of short term stimulation to reduce sarcoplasmic reticulum Ca(2+)-ATPase function in homogenates of rat gastrocnemius.. Mol Cell Biochem 1995 May 10;146(1):23-33.
    pubmed: 7651373doi: 10.1007/bf00926877google scholar: lookup
  4. Duhamel TA, Green HJ, Perco JG, Sandiford SD, Ouyang J. Human muscle sarcoplasmic reticulum function during submaximal exercise in normoxia and hypoxia.. J Appl Physiol (1985) 2004 Jul;97(1):180-7.
    pubmed: 15220318doi: 10.1152/japplphysiol.00954.2003google scholar: lookup
  5. Dux L, Green HJ, Pette D. Chronic low-frequency stimulation of rabbit fast-twitch muscle induces partial inactivation of the sarcoplasmic reticulum Ca2(+)-ATPase and changes in its tryptic cleavage.. Eur J Biochem 1990 Aug 28;192(1):95-100.
    pubmed: 2144818doi: 10.1111/j.1432-1033.1990.tb19200.xgoogle scholar: lookup
  6. Fitts RH, Courtright JB, Kim DH, Witzmann FA. Muscle fatigue with prolonged exercise: contractile and biochemical alterations.. Am J Physiol 1982 Jan;242(1):C65-73.
    pubmed: 6120654doi: 10.1152/ajpcell.1982.242.1.c65google scholar: lookup
  7. Fitts RH. Cellular mechanisms of muscle fatigue.. Physiol Rev 1994 Jan;74(1):49-94.
    pubmed: 8295935doi: 10.1152/physrev.1994.74.1.49google scholar: lookup
  8. Green HJ. How important is endogenous muscle glycogen to fatigue in prolonged exercise?. Can J Physiol Pharmacol 1991 Feb;69(2):290-7.
    pubmed: 2054746doi: 10.1139/y91-045google scholar: lookup
  9. Hicks A, Ohlendieck K, Göpel SO, Pette D. Early functional and biochemical adaptations to low-frequency stimulation of rabbit fast-twitch muscle.. Am J Physiol 1997 Jul;273(1 Pt 1):C297-305.
    pubmed: 9252468doi: 10.1152/ajpcell.1997.273.1.c297google scholar: lookup
  10. Hill CA, Thompson MW, Ruell PA, Thom JM, White MJ. Sarcoplasmic reticulum function and muscle contractile character following fatiguing exercise in humans.. J Physiol 2001 Mar 15;531(Pt 3):871-8.
    pmc: PMC2278486pubmed: 11251066doi: 10.1111/j.1469-7793.2001.0871h.xgoogle scholar: lookup
  11. Inashima S, Matsunaga S, Yasuda T, Wada M. Effect of endurance training and acute exercise on sarcoplasmic reticulum function in rat fast- and slow-twitch skeletal muscles.. Eur J Appl Physiol 2003 Apr;89(2):142-9.
    pubmed: 12665977doi: 10.1007/s00421-002-0763-5google scholar: lookup
  12. Karlsson J, Saltin B. Diet, muscle glycogen, and endurance performance.. J Appl Physiol 1971 Aug;31(2):203-6.
    pubmed: 5558241doi: 10.1152/jappl.1971.31.2.203google scholar: lookup
  13. Lees SJ, Franks PD, Spangenburg EE, Williams JH. Glycogen and glycogen phosphorylase associated with sarcoplasmic reticulum: effects of fatiguing activity.. J Appl Physiol (1985) 2001 Oct;91(4):1638-44.
    pubmed: 11568145doi: 10.1152/jappl.2001.91.4.1638google scholar: lookup
  14. Lees SJ, Williams JH. Skeletal muscle sarcoplasmic reticulum glycogen status influences Ca2+ uptake supported by endogenously synthesized ATP.. Am J Physiol Cell Physiol 2004 Jan;286(1):C97-104.
    pubmed: 12967914doi: 10.1152/ajpcell.00188.2003google scholar: lookup
  15. Li JL, Wang XN, Fraser SF, Carey MF, Wrigley TV, McKenna MJ. Effects of fatigue and training on sarcoplasmic reticulum Ca(2+) regulation in human skeletal muscle.. J Appl Physiol (1985) 2002 Mar;92(3):912-22.
    pubmed: 11842021doi: 10.1152/japplphysiol.00643.2000google scholar: lookup
  16. Maréchal G, Gailly P. Effects of nitric oxide on the contraction of skeletal muscle.. Cell Mol Life Sci 1999 Jul;55(8-9):1088-102.
    pubmed: 10442090doi: 10.1007/s000180050359google scholar: lookup
  17. Mishima T, Yamada T, Matsunaga S, Wada M. N-acetylcysteine fails to modulate the in vitro function of sarcoplasmic reticulum of diaphragm in the final phase of fatigue.. Acta Physiol Scand 2005 Jul;184(3):195-202.
    pubmed: 15954987doi: 10.1111/j.1365-201x.2005.01443.xgoogle scholar: lookup
  18. Mishima T, Sugiyama M, Yamada T, Sakamoto M, Wada M. Effects of reduced glycogen on structure and in vitro function of rat sarcoplasmic reticulum Ca2+-ATPase.. Pflugers Arch 2006 Apr;452(1):117-23.
    pubmed: 16369770doi: 10.1007/s00424-005-0018-5google scholar: lookup
  19. Miyata H, Wada M. Sarcoplasmic reticulum function of rat diaphragm following five-day inactivity. Adv. Exerc. Sports Physiol. 2001 7: 27–32.
  20. O'Brien PJ, Li GO, Locke M, Klabunde RE, Ianuzzo CD. Compensatory up-regulation of cardiac SR Ca2+-pump by heat-shock counteracts SR Ca2+-channel activation by ischemia/reperfusion.. Mol Cell Biochem 1997 Aug;173(1-2):135-43.
    pubmed: 9278264doi: 10.1023/a:1006840013439google scholar: lookup
  21. Pratt SE, Geor RJ, Spriet LL, McCutcheon LJ. Time course of insulin sensitivity and skeletal muscle glycogen synthase activity after a single bout of exercise in horses.. J Appl Physiol (1985) 2007 Sep;103(3):1063-9.
    pubmed: 17585040doi: 10.1152/japplphysiol.01349.2006google scholar: lookup
  22. Riggs CM. Fractures--a preventable hazard of racing thoroughbreds?. Vet J 2002 Jan;163(1):19-29.
    pubmed: 11749133doi: 10.1053/tvjl.2001.0610google scholar: lookup
  23. Rivero JL, Serrano AL, Henckel P, Agüera E. Muscle fiber type composition and fiber size in successfully and unsuccessfully endurance-raced horses.. J Appl Physiol (1985) 1993 Oct;75(4):1758-66.
    pubmed: 8282629doi: 10.1152/jappl.1993.75.4.1758google scholar: lookup
  24. Rivero JL, Talmadge RJ, Edgerton VR. Myosin heavy chain isoforms in adult equine skeletal muscle: an immunohistochemical and electrophoretic study.. Anat Rec 1996 Oct;246(2):185-94.
    pubmed: 8888960doi: 10.1002/(sici)1097-0185(199610)246:2<185::aid-ar5>3.0.co;2-0google scholar: lookup
  25. Rivero JL, Ruz A, Martí-Korff S, Estepa JC, Aguilera-Tejero E, Werkman J, Sobotta M, Lindner A. Effects of intensity and duration of exercise on muscular responses to training of thoroughbred racehorses.. J Appl Physiol (1985) 2007 May;102(5):1871-82.
    pubmed: 17255370doi: 10.1152/japplphysiol.01093.2006google scholar: lookup
  26. Romijn JA, Coyle EF, Sidossis LS, Gastaldelli A, Horowitz JF, Endert E, Wolfe RR. Regulation of endogenous fat and carbohydrate metabolism in relation to exercise intensity and duration.. Am J Physiol 1993 Sep;265(3 Pt 1):E380-91.
    pubmed: 8214047doi: 10.1152/ajpendo.1993.265.3.e380google scholar: lookup
  27. Scherer NM, Deamer DW. Oxidative stress impairs the function of sarcoplasmic reticulum by oxidation of sulfhydryl groups in the Ca2+-ATPase.. Arch Biochem Biophys 1986 May 1;246(2):589-601.
    pubmed: 2939799doi: 10.1016/0003-9861(86)90314-0google scholar: lookup
  28. Schertzer JD, Green HJ, Duhamel TA, Tupling AR. Mechanisms underlying increases in SR Ca2+-ATPase activity after exercise in rat skeletal muscle.. Am J Physiol Endocrinol Metab 2003 Mar;284(3):E597-610.
    pubmed: 12409282doi: 10.1152/ajpendo.00190.2002google scholar: lookup
  29. Serrano AL, Quiroz-Rothe E, Rivero JL. Early and long-term changes of equine skeletal muscle in response to endurance training and detraining.. Pflugers Arch 2000 Dec;441(2-3):263-74.
    pubmed: 11211112doi: 10.1007/s004240000408google scholar: lookup
  30. Simonides WS, van Hardeveld C. An assay for sarcoplasmic reticulum Ca2(+)-ATPase activity in muscle homogenates.. Anal Biochem 1990 Dec;191(2):321-31.
    pubmed: 2150742doi: 10.1016/0003-2697(90)90226-ygoogle scholar: lookup
  31. Tupling AR, Green HJ, Roy BD, Grant S, Ouyang J. Paradoxical effects of prior activity on human sarcoplasmic reticulum Ca2+-ATPase response to exercise.. J Appl Physiol (1985) 2003 Jul;95(1):138-44.
    pubmed: 12794093doi: 10.1152/japplphysiol.00483.2002google scholar: lookup
  32. Vøllestad NK, Vaage O, Hermansen L. Muscle glycogen depletion patterns in type I and subgroups of type II fibres during prolonged severe exercise in man.. Acta Physiol Scand 1984 Dec;122(4):433-41.
    pubmed: 6524389doi: 10.1111/j.1748-1716.1984.tb07531.xgoogle scholar: lookup
  33. Williams JH, Klug GA. Calcium exchange hypothesis of skeletal muscle fatigue: a brief review.. Muscle Nerve 1995 Apr;18(4):421-34.
    pubmed: 7715628doi: 10.1002/mus.880180409google scholar: lookup
  34. Wilson JA, Kronfeld DS, Gay LS, Williams JH, Wilson TM, Lindinger MI. Sarcoplasmic reticulum responses to repeated sprints are affected by conditioning of horses.. J Anim Sci 1998 Dec;76(12):3065-71.
    pubmed: 9928611doi: 10.2527/1998.76123065xgoogle scholar: lookup
  35. Yamano S, Eto D, Sugiura T, Kai M, Hiraga A, Tokuriki M, Miyata H. Effect of growth and training on muscle adaptation in Thoroughbred horses.. Am J Vet Res 2002 Oct;63(10):1408-12.
    pubmed: 12371768doi: 10.2460/ajvr.2002.63.1408google scholar: lookup
  36. Yasuda T, Inashima S, Sasaki S, Kikuchi K, Niihata S, Wada M, Katsuta S. Effects of exhaustive exercise on biochemical characteristics of sarcoplasmic reticulum from rat soleus muscle.. Acta Physiol Scand 1999 Jan;165(1):45-50.
    pubmed: 10072096doi: 10.1046/j.1365-201x.1999.00470.xgoogle scholar: lookup

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