Scintigraphic comparison of intra-arterial injection and distal intravenous regional limb perfusion for administration of mesenchymal stem cells to the equine foot.
Abstract: Intra-arterial (i.a.) and intravenous (i.v.) regional limb perfusions (RLP) through the median artery and cephalic vein, respectively, have been previously investigated for administration of mesenchymal stem cells (MSCs) to the equine distal limb. Limitations due to thrombosis of the arteries after i.a. RLP and poor distribution of MSCs to the foot with i.v. RLP were observed. These techniques need to be modified for clinical use. Objective: Evaluate the distribution, uptake and persistence of radiolabelled MSCs after i.a. injection through the median artery without a tourniquet and after i.v. RLP through the lateral palmar digital vein. Methods: In vivo experimental study. Methods: (99m) Tc-HMPAO-labelled MSCs were injected through the median artery of one limb and the lateral palmar digital vein of the other limb of 6 horses under general anaesthesia. No tourniquet was used for the i.a. injection. A pneumatic tourniquet was placed on the metacarpus for i.v. injection. Scintigraphic images were obtained up to 24 h after injection. Results: Intra-arterial injection resulted in MSCs retention within the limb despite the absence of a tourniquet and no thrombosis was observed. Both i.a. injection and i.v. RLP led to distribution of MSCs to the foot. The i.a. injection resulted in a more homogeneous distribution. The MSC uptake was higher with i.v. RLP at the initial timepoints, but no significant difference was present at 24 h. Conclusions: Both i.a. injection through the median artery without a tourniquet and i.v. RLP performed through the lateral palmar digital vein under general anaesthesia are safe and reliable methods for administration of MSCs to the equine foot. The i.a. technique is preferred owing to the better distribution, but is technically more challenging. The feasibility of performing these techniques on standing horses remains to be investigated.
© 2013 EVJ Ltd.
Publication Date: 2013-10-08 PubMed ID: 23834199DOI: 10.1111/evj.12137Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The study compared two methods of administering stem cells to the foot of a horse, intravenous and intra-arterial injections. The results found that both methods are effective, though the intra-arterial method provides a more comprehensive distribution of cells, despite being a more complex procedure.
Objective of the Research
The objective of this research was to study the distribution, uptake, and persistence of radiolabelled mesenchymal stem cells (MSCs) in equine foot administered via intra-arterial injection and intravenous regional limb perfusion.
Methodology
- The researchers used Tc-HMPAO-labelled MSCs which were injected into the limbs of 6 horses under general anesthesia.
- The injections were done via the median artery of one limb (intra-arterial injection) and the lateral palmar digital vein of the other limb (intravenous regional limb perfusion).
- In the intra-arterial injection, no tourniquet was used, while in the intravenous injection, a pneumatic tourniquet was placed on the metacarpus.
- Scintigraphic images were taken up to 24 hours after the injections to monitor the distribution of the MSCs.
Results of the Study
- The intra-arterial injection resulted in a stable retention of MSCs in the limb, and no thrombosis was observed.
- Both intra-arterial injection and intravenous regional limb perfusion led to the distribution of MSCs to the foot, but the intra-arterial injection resulted in a more evenly distributed dispersion of the cells.
- The initial uptake of MSCs was higher in the case of intravenous regional limb perfusion. However, no significant difference was noticed between the two methods 24 hours after the injection.
Conclusions of the Study
- Both intra-arterial injection and intravenous regional limb perfusion are safe and dependable ways to administer MSCs to a horse’s foot under general anesthesia.
- While intra-arterial method provides a better cell distribution, it is technically more challenging to execute.
- The feasibility of performing these techniques on horses that are standing still needs further exploration.
Cite This Article
APA
Trela JM, Spriet M, Padgett KA, Galuppo LD, Vaughan B, Vidal MA.
(2013).
Scintigraphic comparison of intra-arterial injection and distal intravenous regional limb perfusion for administration of mesenchymal stem cells to the equine foot.
Equine Vet J, 46(4), 479-483.
https://doi.org/10.1111/evj.12137 Publication
Researcher Affiliations
- Veterinary Medical Teaching Hospital, School of Veterinary Medicine, University of California, Davis, USA.
MeSH Terms
- Animals
- Foot / blood supply
- Horses
- Infusions, Intra-Arterial / methods
- Infusions, Intra-Arterial / veterinary
- Infusions, Intravenous / methods
- Infusions, Intravenous / veterinary
- Male
- Mesenchymal Stem Cell Transplantation / methods
- Mesenchymal Stem Cell Transplantation / veterinary
- Radionuclide Imaging / veterinary
- Radiopharmaceuticals / pharmacology
- Technetium Tc 99m Exametazime / pharmacology
Citations
This article has been cited 9 times.- Nascimento C, Saraiva MVA, Pereira VM, de Brito DCC, de Aguiar FLN, Alves BG, Roballo KCS, de Figueiredo JR, Ambrósio CE, Rodrigues APR. Addition of synthetic polymer in the freezing solution of mesenchymal stem cells from equine adipose tissue as a future perspective for reducing of DMSO concentration. Braz J Vet Med 2023;45:e002523.
- Mund SJK, MacPhee DJ, Campbell J, Honaramooz A, Wobeser B, Barber SM. Macroscopic, Histologic, and Immunomodulatory Response of Limb Wounds Following Intravenous Allogeneic Cord Blood-Derived Multipotent Mesenchymal Stromal Cell Therapy in Horses. Cells 2021 Nov 1;10(11).
- Beerts C, Brondeel C, Pauwelyn G, Depuydt E, Tack L, Duchateau L, Xu Y, Saunders JH, Peremans K, Spaas JH. Scintigraphic tracking of (99m)Technetium-labelled equine peripheral blood-derived mesenchymal stem cells after intravenous, intramuscular, and subcutaneous injection in healthy dogs. Stem Cell Res Ther 2021 Jul 13;12(1):393.
- Ribitsch I, Baptista PM, Lange-Consiglio A, Melotti L, Patruno M, Jenner F, Schnabl-Feichter E, Dutton LC, Connolly DJ, van Steenbeek FG, Dudhia J, Penning LC. Large Animal Models in Regenerative Medicine and Tissue Engineering: To Do or Not to Do. Front Bioeng Biotechnol 2020;8:972.
- Voga M, Adamic N, Vengust M, Majdic G. Stem Cells in Veterinary Medicine-Current State and Treatment Options. Front Vet Sci 2020;7:278.
- Mund SJK, Kawamura E, Awang-Junaidi AH, Campbell J, Wobeser B, MacPhee DJ, Honaramooz A, Barber S. Homing and Engraftment of Intravenously Administered Equine Cord Blood-Derived Multipotent Mesenchymal Stromal Cells to Surgically Created Cutaneous Wound in Horses: A Pilot Project. Cells 2020 May 8;9(5).
- Mori da Cunha MGMC, Giacomazzi G, Callewaert G, Hympanova L, Russo F, Vande Velde G, Gijsbers R, Albersen M, Sampaolesi M, Deprest J. Fate of mesoangioblasts in a vaginal birth injury model: influence of the route of administration. Sci Rep 2018 Jul 13;8(1):10604.
- Arzi B, Clark KC, Sundaram A, Spriet M, Verstraete FJM, Walker NJ, Loscar MR, Fazel N, Murphy WJ, Vapniarsky N, Borjesson DL. Therapeutic Efficacy of Fresh, Allogeneic Mesenchymal Stem Cells for Severe Refractory Feline Chronic Gingivostomatitis. Stem Cells Transl Med 2017 Aug;6(8):1710-1722.
- Dudhia J, Becerra P, Valdés MA, Neves F, Hartman NG, Smith RK. In Vivo Imaging and Tracking of Technetium-99m Labeled Bone Marrow Mesenchymal Stem Cells in Equine Tendinopathy. J Vis Exp 2015 Dec 9;(106):e52748.
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