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Equine veterinary journal2013; 46(4); 479-483; doi: 10.1111/evj.12137

Scintigraphic comparison of intra-arterial injection and distal intravenous regional limb perfusion for administration of mesenchymal stem cells to the equine foot.

Abstract: Intra-arterial (i.a.) and intravenous (i.v.) regional limb perfusions (RLP) through the median artery and cephalic vein, respectively, have been previously investigated for administration of mesenchymal stem cells (MSCs) to the equine distal limb. Limitations due to thrombosis of the arteries after i.a. RLP and poor distribution of MSCs to the foot with i.v. RLP were observed. These techniques need to be modified for clinical use. Objective: Evaluate the distribution, uptake and persistence of radiolabelled MSCs after i.a. injection through the median artery without a tourniquet and after i.v. RLP through the lateral palmar digital vein. Methods: In vivo experimental study. Methods: (99m) Tc-HMPAO-labelled MSCs were injected through the median artery of one limb and the lateral palmar digital vein of the other limb of 6 horses under general anaesthesia. No tourniquet was used for the i.a. injection. A pneumatic tourniquet was placed on the metacarpus for i.v. injection. Scintigraphic images were obtained up to 24 h after injection. Results: Intra-arterial injection resulted in MSCs retention within the limb despite the absence of a tourniquet and no thrombosis was observed. Both i.a. injection and i.v. RLP led to distribution of MSCs to the foot. The i.a. injection resulted in a more homogeneous distribution. The MSC uptake was higher with i.v. RLP at the initial timepoints, but no significant difference was present at 24 h. Conclusions: Both i.a. injection through the median artery without a tourniquet and i.v. RLP performed through the lateral palmar digital vein under general anaesthesia are safe and reliable methods for administration of MSCs to the equine foot. The i.a. technique is preferred owing to the better distribution, but is technically more challenging. The feasibility of performing these techniques on standing horses remains to be investigated.
© 2013 EVJ Ltd.
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Publication Date: 2013-10-08 PubMed ID: 23834199DOI: 10.1111/evj.12137Google Scholar: Lookup
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  • Journal Article
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study compared two methods of administering stem cells to the foot of a horse, intravenous and intra-arterial injections. The results found that both methods are effective, though the intra-arterial method provides a more comprehensive distribution of cells, despite being a more complex procedure.

Objective of the Research

The objective of this research was to study the distribution, uptake, and persistence of radiolabelled mesenchymal stem cells (MSCs) in equine foot administered via intra-arterial injection and intravenous regional limb perfusion.

Methodology

  • The researchers used Tc-HMPAO-labelled MSCs which were injected into the limbs of 6 horses under general anesthesia.
  • The injections were done via the median artery of one limb (intra-arterial injection) and the lateral palmar digital vein of the other limb (intravenous regional limb perfusion).
  • In the intra-arterial injection, no tourniquet was used, while in the intravenous injection, a pneumatic tourniquet was placed on the metacarpus.
  • Scintigraphic images were taken up to 24 hours after the injections to monitor the distribution of the MSCs.

Results of the Study

  • The intra-arterial injection resulted in a stable retention of MSCs in the limb, and no thrombosis was observed.
  • Both intra-arterial injection and intravenous regional limb perfusion led to the distribution of MSCs to the foot, but the intra-arterial injection resulted in a more evenly distributed dispersion of the cells.
  • The initial uptake of MSCs was higher in the case of intravenous regional limb perfusion. However, no significant difference was noticed between the two methods 24 hours after the injection.

Conclusions of the Study

  • Both intra-arterial injection and intravenous regional limb perfusion are safe and dependable ways to administer MSCs to a horse’s foot under general anesthesia.
  • While intra-arterial method provides a better cell distribution, it is technically more challenging to execute.
  • The feasibility of performing these techniques on horses that are standing still needs further exploration.

Cite This Article

APA
Trela JM, Spriet M, Padgett KA, Galuppo LD, Vaughan B, Vidal MA. (2013). Scintigraphic comparison of intra-arterial injection and distal intravenous regional limb perfusion for administration of mesenchymal stem cells to the equine foot. Equine Vet J, 46(4), 479-483. https://doi.org/10.1111/evj.12137

Publication

Equine veterinary journal
ISSN: 2042-3306
NlmUniqueID: 0173320
Country: United States
Language: English
Volume: 46
Issue: 4
Pages: 479-483

Researcher Affiliations

Trela, J M
  • Veterinary Medical Teaching Hospital, School of Veterinary Medicine, University of California, Davis, USA.
Spriet, M
    Padgett, K A
      Galuppo, L D
        Vaughan, B
          Vidal, M A

            MeSH Terms

            • Animals
            • Foot / blood supply
            • Horses
            • Infusions, Intra-Arterial / methods
            • Infusions, Intra-Arterial / veterinary
            • Infusions, Intravenous / methods
            • Infusions, Intravenous / veterinary
            • Male
            • Mesenchymal Stem Cell Transplantation / methods
            • Mesenchymal Stem Cell Transplantation / veterinary
            • Radionuclide Imaging / veterinary
            • Radiopharmaceuticals / pharmacology
            • Technetium Tc 99m Exametazime / pharmacology

            Citations

            This article has been cited 9 times.
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