Seasonal Changes in Trace-Element Content in the Coat of Hucul Horses.
Abstract: The purpose of the study was to evaluate seasonal changes in selected trace elements such as Fe, Cu, Mn, Zn, and Al in the coat of healthy Hucul horses kept in south-eastern Poland in two different facilities and fed with locally sourced feed. The coat for the study was collected from 24 individuals in autumn, winter, and spring. The concentration of elements in the feed was also determined. The date of collection had a significant effect on the concentration of the micronutrients analysed in the coat of Hucul horses. The highest concentration of Zn was found in the coat taken in summer. The coat taken in autumn had the highest concentrations of Fe, Cu, Mn, and Al compared with the other seasons. The highest concentrations of Fe, Mn, and Al were found in fur taken in winter, with the lowest levels of Zn. Positive correlations were found between the content of iron and manganese, iron and aluminium, and manganese and aluminium in the coat of Hucul horses. A clear inter-individual and inter-stable variability was found, which may indicate the need for further research that also takes into account other factors.
Publication Date: 2022-10-14 PubMed ID: 36290155PubMed Central: PMC9597826DOI: 10.3390/ani12202770Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This research investigates the seasonal variations in the concentrations of specific trace elements found in the coat of Hucul horses from southeast Poland.
Objective of the Research
- The main objective of the research was to study the seasonal changes in selected trace elements such as Iron (Fe), Copper (Cu), Manganese (Mn), Zinc (Zn), and Aluminium (Al) in the coat of healthy Hucul horses that were living in two different facilities in southeastern Poland.
Research Methodology
- Horse coat samples were collected from 24 healthy individuals during three different seasons- autumn, winter, and spring.
- The concentration of specific elements in the horses’ locally-sourced feed was also determined.
- The researchers then analyzed the variations in the concentration of these trace elements across these seasons.
Findings of the Research
- The study concluded that the season or time of year when the fur was collected had a significant influence on the trace element concentrations in the coat of Hucul horses.
- The highest concentration of Zinc (Zn) was found in the fur samples collected during summer.
- Fur samples gathered during autumn showed the greatest concentrations of Iron (Fe), Copper (Cu), Manganese (Mn), and Aluminium (Al), as compared to other seasons.
- The coats fetched during winter months displayed the highest concentrations of Iron (Fe), Manganese (Mn), and Aluminium (Al), but the lowest level of Zinc (Zn).
- This indicates that the elemental composition of a horse’s fur may be indicative of their nutritional status, which varies throughout the year.
- Significant positive correlations were observed between the concentrations of Iron and Manganese, Iron and Aluminium, as well as Manganese and Aluminium, in the horse’s fur.
Implications of the Research
- The research provides important insights into how the trace element content in the horses’ coats could potentially vary across different seasons and based on their diet.
- The findings suggest there might be unique, individual, and stable-specific variability in the trace element concentration in the Hucul horses’ fur.
- This highlights the potential necessity for conducting more elaborate future researches that could also consider additional factors.
Cite This Article
APA
Jachimowicz-Rogowska K, Topczewska J, Krupa W, Bajcar M, Kwiecień M, Winiarska-Mieczan A.
(2022).
Seasonal Changes in Trace-Element Content in the Coat of Hucul Horses.
Animals (Basel), 12(20).
https://doi.org/10.3390/ani12202770 Publication
Researcher Affiliations
- Department of Bromatology and Food Physiology, Institute of Animal Nutrition and Bromatology, University of Life Sciences in Lublin, Akademicka Str. 13, 20-950 Lublin, Poland.
- Department of Animal Production and Poultry Products Evaluation, College of Natural Sciences, University of Rzeszów, Zelwerowicza Str. 4, 35-601 Rzeszow, Poland.
- Department of Animal Ethology and Wildlife Management, University of Life Sciences in Lublin, Akademicka Str. 13, 20-950 Lublin, Poland.
- Department of Bioenergetics, Food Analysis and Microbiology, College of Natural Sciences, University of Rzeszów, Ćwiklińskiej St. 2D, 35-601 Rzeszow, Poland.
- Department of Animal Nutrition, Institute of Animal Nutrition and Bromatology, University of Life Sciences in Lublin, Akademicka Str. 13, 20-950 Lublin, Poland.
- Department of Bromatology and Food Physiology, Institute of Animal Nutrition and Bromatology, University of Life Sciences in Lublin, Akademicka Str. 13, 20-950 Lublin, Poland.
Conflict of Interest Statement
The authors declare no conflict of interest.
References
This article includes 53 references
- Meyer W, Schnapper A, Hülmann G. The hair cuticle of mammals and its relationship to functions of the hair coat.. J. Zool. 2002;256:489–494.
- Dunnett M. The diagnostic potential of equine hair: A comparative review of hair analysis for assessing nutritional status, environmental poisoning, and drug use and abuse.. Advances in equine nutrition-III 2005;pp. 85–106.
- O’Connor K, Goldberg L.J. Nutrition and hair.. Clin. Dermatol. 2021;39:809–818.
- Kienzle E, Zorn N. Bioavailability of minerals in the horse. Proceedings of the 3rd European Equine Nutrition & Health Congress Ghent, Belgium. 17–18 March 2006.
- Almohanna H.M, Ahmed A.A, Tsatalis J.P, Tosti A. The role of vitamins and minerals in hair loss: A review.. Dermatol. Ther. 2019;9:51–70.
- Jutha N, Jardine C, Schwantje H, Mosbacher J, Kinniburgh D, Kutz S. Evaluating the use of hair as a non-invasive indicator of trace mineral status in woodland caribou (Rangifer tarandus caribou). PLoS ONE 2022;17:e0269441.
- Siwińska N, Żak A, Słowikowska M, Kubiak K, Jaworski Z, Niedzwiedź A. Morphology and elemental analysis of free range and stabled Polish Konik horses hair using Energy-dispersive X-ray spectroscopy (EDS). Polish J. Vet. Sci. 2018;21:65–72.
- Stanek M, Jaworski Z, Sobotka W, Lipiński K, Olenkowicz R. Influence of an organic supplement of copper, zinc and manganese in feed rations on concentrations of these elements in the coat of Polish Konik horses.. J. Elem. 2016;21:549–558.
- Piccione G, Assenza A, Fazio F, Bergero D, Caola G. Daily rhythms of serum vitamin D-metabolites, calcium and phosphorus in horses.. Acta Vet. Brno. 2008;77:151–157.
- Pourmohammad R, Mohri M, Seifi H, Sardari K. Effect of exercise on some minerals, metabolites and enzyme activities in the serum of trained Arabian horses.. Turk. J. Vet. Anim. Sci. 2019;43:791–799.
- Cygan-Szczegielniak D, Stanek M, Stasiak K, Roślewska A, Janicki B. The Content of Mineral Elements and Heavy Metals in the Hair of Red Deer (Cervus elaphus L.) from Selected Regions of Poland.. Folia Biol.-Krakow. 2018;66:133–142.
- Khudzari M.D, Wagiran H, Hossain I, Ibrahim N. Screening heavy metals levels in hair of sanitation workers by X-ray fluorescence analysis.. J. Environ. Radioact. 2013;115:1–5.
- Jaworski Z, Stanek M, Sobotka W. Mineral composition of the hair coat of Polish Konik horses raised in nature reserves and barns.. J. Elem. 2017;22:857–867.
- Cygan-Szczegielniak D. The Levels of Mineral Elements and Toxic Metals in the Longissimus lumborum Muscle, Hair and Selected Organs of Red Deer (Cervus elaphus L.) in Poland.. Animals 2021;11:1231.
- Fazio F, Gugliandolo E, Nava V, Piccione G, Giannetto C, Licata P. Bioaccumulation of Mineral Elements in Different Biological Substrates of Athletic Horse from Messina, Italy.. Animals 2020;10:1877.
- Montillo M, Caslini C, Peric T, Prandi A, Netto P, Tubaro F, Pedrotti L, Bianchi A, Mattiello S. Analysis of 19 minerals and cortisol in red deer hair in two different areas of the stelvio national park: A preliminary study.. Animals 2019;9:492.
- Gil-Jiménez E, Mateo R, de Lucas M, Ferrer M. Feathers and hair as tools for non-destructive pollution exposure assessment in a mining site of the Iberian Pyrite Belt.. Environ. Pollut. 2020;263:114523.
- Carrol C, Huntington P. Body condition scoring and weight estimation of horses.. Equine Vet. J. 1988;20:41–45.
- Dalla Costa E, Murray L, Dai F, Canali E, Minero M. Equine on-farm welfare assessment: A review of animal-based indicators.. Anim. Welf. 2014;23:323–341.
- Rowland M, Coombs T, Connor M. A study of traveller horse owners’ attitudes to horse care and welfare using an equine Body Condition Scoring system.. Animals 2019;9:162.
- Návesník J, Krejčová A, Černohorský T, Pátková A, Petrovická I. High throughput method for multielemental analysis of horse hair by oaTOF-ICP-MS.. Chem. Pap. 2017;71:991–998.
- Hoskin S.O, Gee E.K. Feeding value of pastures for horses.. N. Z. Vet. J. 2004;52:332–341.
- Saastamoinen M.T, Hellämäki M. Forages and Grazing in Horse Nutrition.. Wageningen Academic Publishers 2012;pp. 305–314.
- Harris P.A, Ellis A.D, Fradinho M.J, Jansson A, Julliand V, Luthersson N, Vervuert I. Feeding conserved forage to horses: Recent advances and recommendations.. Animal 2017;11:958–967.
- Müller C.E. Silage and haylage for horses.. Grass Forage Sci. 2018;73:815–827.
- Smith A.D, Panickar K.S, Urban J.F. Jr, Dawson H.D. Impact of micronutrients on the immune response of animals.. Annu. Rev. Anim. Biosci. 2018;6:227–254.
- Meyer H, Coenen M. Pferdefütterung.. Georg Thieme Verlag 2002;No. 2.
- Zhao X, Müller C.E. Macro-and micromineral content of wrapped forages for horses.. Grass Forage Sci. 2016;71:195–207.
- Johanesson T, Eriksson T, Gudmundsdóttir K.B, Sigurdarsson S, Kristinsson J. Overview: Seven trace elements in Icelandic forage. Their value in animal health and with special relation to scrapie.. Icel. J. Agric. Sci. 2007;20:3–24.
- Lindström B.E.M, Frankow-Lindberg B.E, Dahlin A.S, Wivstad M, Watson C.A. Micronutrient concentrations in common and novel forage species and varieties grown on two contrasting soils.. Grass Forage Sci. 2013;68:427–436.
- Pirhofer-Walzl K, Søegaard K, Høgh-Jensen H, Eriksen J, Sanderson M.A, Rasmussen J, Rasmussen J. Forage herbs improve mineral composition of grassland herbage.. Grass Forage Sci. 2011;66:415–423.
- Cygan-Szczegielniak D, Stanek M, Giernatowska E, Janicki B. Impact of breeding region and season on the content of some trace elements and heavy metals in the hair of cows.. Folia Biol. 2014;62:163–169.
- Brummer-Holder M, Cassill B.D, Hayes S.H. Interrelationships between age and trace element concentration in horse mane hair and whole blood.. J. Equine Vet. Sci. 2020;87:102922.
- Nageeb Rashed M, Soltan M.E. Animal hair as biological indicator for heavy metal pollution in urban and rural areas.. Environ. Monitor. Assess. 2005;110:41–53.
- Souza M.V.D, Fontes M.P.F, Fernandes R.B.A. Heavy metals in equine biological components.. Rev. Bras. Zootecn. 2014;43:60–66.
- Truchliński J, Budzyński M, Rzucidło M. Dietary supplementation of minerals for horses based on the analysis of the composition of elements contained in the hair coat.. Ann. UMCS Lublin Sect. EE. 2004;22:263–270.
- Topczewska J. Effects of seasons on the concentration of selected trace elements in horse hair.. J. Cent. Eur. Agric. 2012;13:671–680.
- Biricik H, Ocal N, Gucus A.I, Ediz B, Uzman M. Seasonal changes of some mineral status in mares.. J. Equine Vet. Sci. 2005;25:346–348.
- Madejon P, Dominguez M.T, Murillo J.M. Evaluation of pastures for horses grazing on soils polluted by trace elements.. Ecotoxicology 2009;18:417–428.
- Pajarillo E.A.B, Lee E, Kang D.K. Trace metals and animal health: Interplay of the gut microbiota with iron, manganese, zinc, and copper.. Anim. Nutr. 2021;7:750–761.
- Tirpák F, Halo M. Jr, Tokárová K, Binkowski L.J, Vašíček J, Svoradová A, Błaszczyk-Altman M, Kováčik A, Tvrdá E, Chrenek P. Composition of stallion seminal plasma and its impact on oxidative stress markers and spermatozoa quality.. Life 2021;11:1238.
- Hill G.M, Shannon M. Copper and zinc nutritional issues for agricultural animal production.. Biol. Trace Elem. Res. 2019;188:148–159.
- Hallamaa R, Peraniemi S. Trace Element Analysis in Sera of Horses with Allergic Dermatitis and in Matched Healthy Controls with Special Attention to Zn, Ni and Ti.. Open J. Anim. Sci. 2022;12:129–144.
- Paßlack N, van Bömmel-Wegmann S, Vahjen W, Zentek J. Impact of Dietary Zinc Chloride Hydroxide and Zinc Methionine on the Faecal Microbiota of Healthy Adult Horses and Ponies.. J. Equine Vet. Sci. 2022;110:103804.
- Youssef M.A, El-khodery S.A, Ibrahim H.M.M. Antioxidant Trace Elements in Serum of Draft Horses with Acute and Chronic Lower Airway Disease.. Biol. Trace Elem. Res. 2012;150:123–129.
- Sgorlon S, Mattiello A, Ronutti L, Sandri M, Stefanon B. Concentration of elements in the hair of growing and adult dogs.. Ital. J. Anim. Sci. 2019;18:1126–1134.
- Kalashnikov V, Zajcev A, Atroshchenko M, Miroshnikov S, Frolov A, Zav’yalov O, Kalinkova L, Kalashnikova T. The content of essential and toxic elements in the hair of the mane of the trotter horses depending on their speed.. Environ. Sci. Pollut. Res. 2018;25:21961–21967.
- Długaszek M. Studies on relationships between essential and toxic elements in selected body fluids, cells and tissues.. Chem. Biol. Interact. 2019;297:57–66.
- Goff J.P. Invited review: Mineral absorption mechanisms, mineral interactions that affect acid–base and antioxidant status, and diet considerations to improve mineral status.. J. Dairy Sci. 2018;101:2763–2813.
- Van Emon M, Sanford C, McCoski S. Impacts of bovine trace mineral supplementation on maternal and offspring production and health.. Animals 2020;10:2404.
- Byrne L, Murphy R.A. Relative Bioavailability of Trace Minerals in Production Animal Nutrition: A Review.. Animals 2022;12:1981.
- Ha J.H, Doguer C, Collins J.F. Consumption of a high-iron diet disrupts homeostatic regulation of intestinal copper absorption in adolescent mice.. Am. J. Physiol. Gastrointest. Liver Physiol. 2017;313:G353–G360.
- Asano R, Suzuki K, Otsuka T, Otsuka M, Sakurai H. Concentrations of toxic metals and essential minerals in the mane hair of healthy racing horses and their relation to age.. J. Vet. Med. Sci. 2002;64:607–610.
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists