Secretoglobin and Transferrin Expression in Bronchoalveolar Lavage Fluid of Horses with Chronic Respiratory Disease.
Abstract: Lower expression of secretoglobin and transferrin has been found in the bronchoalveolar lavage fluid (BALF) of a small number of horses with experimentally induced signs of recurrent airway obstruction (RAO) compared to healthy controls. Objective: Secretoglobin and transferrin BALF expression will be similarly decreased in horses with naturally occurring clinical signs of RAO and in horses with experimentally induced clinical signs of RAO as compared to healthy controls and intermediate in horses with inflammatory airway disease (IAD). Methods: Recurrent airway obstruction-affected and control horses were subjected to an experimental hay exposure trial to induce signs of RAO. Client-owned horses with a presumptive diagnosis of RAO and controls from the same stable environments were recruited. Methods: Pulmonary function and BALF were evaluated from control and RAO-affected research horses during an experimental hay exposure trial (n = 5 in each group) and from client-owned horses (RAO-affected horses, n = 17; IAD-affected horses, n = 19; healthy controls, n = 5). The concentrations of secretoglobin and transferrin in BALF were assessed using Western blots. Results: Naturally occurring and experimentally induced RAO horses had similar decreases in BALF transferrin expression, but secretoglobin expression was most decreased in naturally occurring RAO. Secretoglobin and transferrin expression were both lower in BALF of RAO-affected horses than in IAD-affected and control horses. Conclusions: Secretoglobin and transferrin expression is decreased in BALF of RAO-affected horses after both experimental and natural exposure. Secretoglobin and transferrin likely play clinically relevant roles in the pathophysiology of RAO, and may thus be used as biomarkers of the disease.
Copyright © 2015 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals, Inc. on behalf of American College of Veterinary Internal Medicine.
Publication Date: 2015-08-30 PubMed ID: 26332291PubMed Central: PMC4895674DOI: 10.1111/jvim.13604Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
- Airway Disease
- Biomarkers
- Bronchoalveolar Lavage
- Clinical Pathology
- Clinical Signs
- Comparative Study
- Diagnosis
- Disease Diagnosis
- Equine Health
- Experimental Methods
- Horses
- Inflammation
- Pathogenesis
- Pathophysiology
- Pulmonary Health
- Recurrent Airway Obstruction
- Respiratory Disease
- Secretion
- Transferrin
- Veterinary Medicine
- Western Blot
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The research study investigates the decreased levels of secretoglobin and transferrin, two proteins present in bronchoalveolar lavage fluid (BALF), in horses with recurrent airway obstruction (RAO), a chronic respiratory disease. The decrease in these protein levels, found consistently in both naturally occurring and experimentally induced RAO-affected horses, suggests a potential role in disease development and could act as a biomarker for diagnosis.
Objective
- The main objective of the study is to determine whether the levels of secretoglobin and transferrin in BALF are similarly decreased in horses with naturally occurring recurrent airway obstruction (RAO), as well as those with experimentally induced symptoms of the disease, compared to healthy control horses. The study also examines these protein levels in horses suffering from inflammatory airway disease (IAD), expecting intermediate results between healthy and RAO-affected horses.
Methods
- For the experimental part of the study, both control horses and those affected by RAO were subjected to a trial that involved exposing them to hay, which is known to induce symptoms of RAO.
- In addition, the study included client-owned horses presumptively diagnosed with RAO and controls from the same stables. In total, five research horses were assigned to each of the control and RAO-affected groups for the hay exposure trial. Additionally, 17 RAO-affected horses, 19 IAD-affected horses and, five healthy controls were studied from client-owned stables.
- Methods focussed on evaluating pulmonary function and BALF in all participating horses. The concentrations of secretoglobin and transferrin in BALF were specifically measured and compared using Western blots, a technique used to detect specific protein levels.
Results
- The results revealed comparable declines in BALF transferrin expression in both naturally occurring and experimentally induced RAO horses.
- However, the decrease in secretoglobin expression was most significant in naturally occurring RAO horses.
- Both secretoglobin and transferrin levels were lower in the BALF of RAO-affected horses than in those affected with IAD and in control horses.
Conclusions
- The study concluded that the expression of secretoglobin and transferrin is decreased in the BALF of RAO-affected horses, regardless of whether the exposure is experimental or natural.
- It further suggests that secretoglobin and transferrin possibly play clinically important roles in RAO pathophysiology and could potentially be used as biomarkers for the disease.
Cite This Article
APA
Miskovic Feutz M, Couetil LL, Riley CP, Zhang X, Adamec J, Raskin RE.
(2015).
Secretoglobin and Transferrin Expression in Bronchoalveolar Lavage Fluid of Horses with Chronic Respiratory Disease.
J Vet Intern Med, 29(6), 1692-1699.
https://doi.org/10.1111/jvim.13604 Publication
Researcher Affiliations
- Department of Veterinary Clinical Sciences, Purdue University College of Veterinary Medicine, West Lafayette, IN.
- Department of Veterinary Clinical Sciences, Purdue University College of Veterinary Medicine, West Lafayette, IN.
- Bindley Bioscience Center, Purdue University, West Lafayette, IN.
- Department of Chemistry, University of Louisville, Louisville, KY.
- Bindley Bioscience Center, Purdue University, West Lafayette, IN.
- Department of Comparative Pathobiology, Purdue University College of Veterinary Medicine, West Lafayette, IN.
MeSH Terms
- Animals
- Bronchoalveolar Lavage Fluid / chemistry
- Chronic Disease
- Gene Expression Regulation
- Horses / metabolism
- Lung Diseases, Obstructive / metabolism
- Lung Diseases, Obstructive / veterinary
- Secretoglobins / chemistry
- Secretoglobins / genetics
- Secretoglobins / metabolism
- Transferrin / analysis
- Transferrin / genetics
- Transferrin / metabolism
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Citations
This article has been cited 3 times.- Padoan E, Ferraresso S, Pegolo S, Barnini C, Castagnaro M, Bargelloni L. Gene Expression Profiles of the Immuno-Transcriptome in Equine Asthma. Animals (Basel) 2022 Dec 20;13(1).
- Gy C, Leclere M, Vargas A, Grimes C, Lavoie JP. Investigation of blood biomarkers for the diagnosis of mild to moderate asthma in horses. J Vet Intern Med 2019 Jul;33(4):1789-1795.
- Lendl L, Barton AK. Equine Asthma Diagnostics: Review of Influencing Factors and Difficulties in Diagnosing Subclinical Disease. Animals (Basel) 2024 Dec 4;14(23).
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