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Secretoglobin and Transferrin Expression in Bronchoalveolar Lavage Fluid of Horses with Chronic Respiratory Disease.
Abstract: Lower expression of secretoglobin and transferrin has been found in the bronchoalveolar lavage fluid (BALF) of a small number of horses with experimentally induced signs of recurrent airway obstruction (RAO) compared to healthy controls. Objective: Secretoglobin and transferrin BALF expression will be similarly decreased in horses with naturally occurring clinical signs of RAO and in horses with experimentally induced clinical signs of RAO as compared to healthy controls and intermediate in horses with inflammatory airway disease (IAD). Methods: Recurrent airway obstruction-affected and control horses were subjected to an experimental hay exposure trial to induce signs of RAO. Client-owned horses with a presumptive diagnosis of RAO and controls from the same stable environments were recruited. Methods: Pulmonary function and BALF were evaluated from control and RAO-affected research horses during an experimental hay exposure trial (n = 5 in each group) and from client-owned horses (RAO-affected horses, n = 17; IAD-affected horses, n = 19; healthy controls, n = 5). The concentrations of secretoglobin and transferrin in BALF were assessed using Western blots. Results: Naturally occurring and experimentally induced RAO horses had similar decreases in BALF transferrin expression, but secretoglobin expression was most decreased in naturally occurring RAO. Secretoglobin and transferrin expression were both lower in BALF of RAO-affected horses than in IAD-affected and control horses. Conclusions: Secretoglobin and transferrin expression is decreased in BALF of RAO-affected horses after both experimental and natural exposure. Secretoglobin and transferrin likely play clinically relevant roles in the pathophysiology of RAO, and may thus be used as biomarkers of the disease.
Copyright © 2015 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals, Inc. on behalf of American College of Veterinary Internal Medicine.
Publication Date: 2015-08-30 PubMed ID: 26332291PubMed Central: PMC4895674DOI: 10.1111/jvim.13604Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
- Airway Disease
- Biomarkers
- Bronchoalveolar Lavage
- Clinical Pathology
- Clinical Signs
- Comparative Study
- Diagnosis
- Disease Diagnosis
- Equine Health
- Experimental Methods
- Horses
- Inflammation
- Pathogenesis
- Pathophysiology
- Pulmonary Health
- Recurrent Airway Obstruction
- Respiratory Disease
- Secretion
- Transferrin
- Veterinary Medicine
- Western Blot
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The research study investigates the decreased levels of secretoglobin and transferrin, two proteins present in bronchoalveolar lavage fluid (BALF), in horses with recurrent airway obstruction (RAO), a chronic respiratory disease. The decrease in these protein levels, found consistently in both naturally occurring and experimentally induced RAO-affected horses, suggests a potential role in disease development and could act as a biomarker for diagnosis.
Objective
- The main objective of the study is to determine whether the levels of secretoglobin and transferrin in BALF are similarly decreased in horses with naturally occurring recurrent airway obstruction (RAO), as well as those with experimentally induced symptoms of the disease, compared to healthy control horses. The study also examines these protein levels in horses suffering from inflammatory airway disease (IAD), expecting intermediate results between healthy and RAO-affected horses.
Methods
- For the experimental part of the study, both control horses and those affected by RAO were subjected to a trial that involved exposing them to hay, which is known to induce symptoms of RAO.
- In addition, the study included client-owned horses presumptively diagnosed with RAO and controls from the same stables. In total, five research horses were assigned to each of the control and RAO-affected groups for the hay exposure trial. Additionally, 17 RAO-affected horses, 19 IAD-affected horses and, five healthy controls were studied from client-owned stables.
- Methods focussed on evaluating pulmonary function and BALF in all participating horses. The concentrations of secretoglobin and transferrin in BALF were specifically measured and compared using Western blots, a technique used to detect specific protein levels.
Results
- The results revealed comparable declines in BALF transferrin expression in both naturally occurring and experimentally induced RAO horses.
- However, the decrease in secretoglobin expression was most significant in naturally occurring RAO horses.
- Both secretoglobin and transferrin levels were lower in the BALF of RAO-affected horses than in those affected with IAD and in control horses.
Conclusions
- The study concluded that the expression of secretoglobin and transferrin is decreased in the BALF of RAO-affected horses, regardless of whether the exposure is experimental or natural.
- It further suggests that secretoglobin and transferrin possibly play clinically important roles in RAO pathophysiology and could potentially be used as biomarkers for the disease.
Cite This Article
APA
Miskovic Feutz M, Couetil LL, Riley CP, Zhang X, Adamec J, Raskin RE.
(2015).
Secretoglobin and Transferrin Expression in Bronchoalveolar Lavage Fluid of Horses with Chronic Respiratory Disease.
J Vet Intern Med, 29(6), 1692-1699.
https://doi.org/10.1111/jvim.13604 Publication
Researcher Affiliations
- Department of Veterinary Clinical Sciences, Purdue University College of Veterinary Medicine, West Lafayette, IN.
- Department of Veterinary Clinical Sciences, Purdue University College of Veterinary Medicine, West Lafayette, IN.
- Bindley Bioscience Center, Purdue University, West Lafayette, IN.
- Department of Chemistry, University of Louisville, Louisville, KY.
- Bindley Bioscience Center, Purdue University, West Lafayette, IN.
- Department of Comparative Pathobiology, Purdue University College of Veterinary Medicine, West Lafayette, IN.
MeSH Terms
- Animals
- Bronchoalveolar Lavage Fluid / chemistry
- Chronic Disease
- Gene Expression Regulation
- Horses / metabolism
- Lung Diseases, Obstructive / metabolism
- Lung Diseases, Obstructive / veterinary
- Secretoglobins / chemistry
- Secretoglobins / genetics
- Secretoglobins / metabolism
- Transferrin / analysis
- Transferrin / genetics
- Transferrin / metabolism
References
This article includes 25 references
- Leclere M, Lavoie-Lamoureux A, Lavoie JP. Heaves, an asthma-like disease of horses.. Respirology 2011 Oct;16(7):1027-46.
- Ramseyer A, Gaillard C, Burger D, Straub R, Jost U, Boog C, Marti E, Gerber V. Effects of genetic and environmental factors on chronic lower airway disease in horses.. J Vet Intern Med 2007 Jan-Feb;21(1):149-56.
- Couëtil LL, Hoffman AM, Hodgson J, Buechner-Maxwell V, Viel L, Wood JL, Lavoie JP. Inflammatory airway disease of horses.. J Vet Intern Med 2007 Mar-Apr;21(2):356-61.
- Bright LA, Mujahid N, Nanduri B, McCarthy FM, Costa LR, Burgess SC, Swiderski CE. Functional modelling of an equine bronchoalveolar lavage fluid proteome provides experimental confirmation and functional annotation of equine genome sequences.. Anim Genet 2011 Aug;42(4):395-405.
- Feutz MM, Riley CP, Zhang X. Proteomic analysis of bronchoalveolar lavage fluid in an equine model of asthma during a natural antigen exposure trial. J Integrated Omics 2012;2:123–131.
- Couëtil LL, Rosenthal FS, Simpson CM. Forced expiration: a test for airflow obstruction in horses.. J Appl Physiol (1985) 2000 May;88(5):1870-9.
- Tesarowski DB, Viel L, McDonell WN. Pulmonary function measurements during repeated environmental challenge of horses with recurrent airway obstruction (heaves).. Am J Vet Res 1996 Aug;57(8):1214-9.
- Miskovic M, Couëtil LL, Thompson CA. Lung function and airway cytologic profiles in horses with recurrent airway obstruction maintained in low-dust environments.. J Vet Intern Med 2007 Sep-Oct;21(5):1060-6.
- Shijubo N, Itoh Y, Yamaguchi T, Sugaya F, Hirasawa M, Yamada T, Kawai T, Abe S. Serum levels of Clara cell 10-kDa protein are decreased in patients with asthma.. Lung 1999;177(1):45-52.
- Ye Q, Fujita M, Ouchi H, Inoshima I, Maeyama T, Kuwano K, Horiuchi Y, Hara N, Nakanishi Y. Serum CC-10 in inflammatory lung diseases.. Respiration 2004 Sep-Oct;71(5):505-10.
- Snyder JC, Reynolds SD, Hollingsworth JW, Li Z, Kaminski N, Stripp BR. Clara cells attenuate the inflammatory response through regulation of macrophage behavior.. Am J Respir Cell Mol Biol 2010 Feb;42(2):161-71.
- Vesterberg O, Palmberg L, Larsson K. Albumin, transferrin and alpha2-macroglobulin in bronchoalveolar lavage fluid following exposure to organic dust in healthy subjects.. Int Arch Occup Environ Health 2001 May;74(4):249-54.
- Katavolos P, Ackerley CA, Viel L, Clark ME, Wen X, Bienzle D. Clara cell secretory protein is reduced in equine recurrent airway obstruction.. Vet Pathol 2009 Jul;46(4):604-13.
- Katavolos P, Ackerley CA, Clark ME, Bienzle D. Clara cell secretory protein increases phagocytic and decreases oxidative activity of neutrophils.. Vet Immunol Immunopathol 2011 Jan;139(1):1-9.
- Shijubo N, Itoh Y, Yamaguchi T, Imada A, Hirasawa M, Yamada T, Kawai T, Abe S. Clara cell protein-positive epithelial cells are reduced in small airways of asthmatics.. Am J Respir Crit Care Med 1999 Sep;160(3):930-3.
- Wang SZ, Rosenberger CL, Bao YX, Stark JM, Harrod KS. Clara cell secretory protein modulates lung inflammatory and immune responses to respiratory syncytial virus infection.. J Immunol 2003 Jul 15;171(2):1051-60.
- Wang SZ, Rosenberger CL, Espindola TM, Barrett EG, Tesfaigzi Y, Bice DE, Harrod KS. CCSP modulates airway dysfunction and host responses in an Ova-challenged mouse model.. Am J Physiol Lung Cell Mol Physiol 2001 Nov;281(5):L1303-11.
- Côté O, Lillie BN, Hayes MA, Clark ME, van den Bosch L, Katavolos P, Viel L, Bienzle D. Multiple secretoglobin 1A1 genes are differentially expressed in horses.. BMC Genomics 2012 Dec 19;13:712.
- Côté O, Clark ME, Viel L, Labbé G, Seah SY, Khan MA, Douda DN, Palaniyar N, Bienzle D. Secretoglobin 1A1 and 1A1A differentially regulate neutrophil reactive oxygen species production, phagocytosis and extracellular trap formation.. PLoS One 2014;9(4):e96217.
- Stites SW, Nelson ME, Wesselius LJ. Transferrin concentrations in serum and lower respiratory tract fluid of mechanically ventilated patients with COPD or ARDS.. Chest 1995 Jun;107(6):1681-5.
- Brogan TD, Ryley HC, Neale L, Yassa J. Soluble proteins of bronchopulmonary secretions from patients with cystic fibrosis, asthma, and bronchitis.. Thorax 1975 Feb;30(1):72-9.
- Hur GY, Choi GS, Sheen SS, Lee HY, Park HJ, Choi SJ, Ye YM, Park HS. Serum ferritin and transferrin levels as serologic markers of methylene diphenyl diisocyanate-induced occupational asthma.. J Allergy Clin Immunol 2008 Oct;122(4):774-780.
- Kirschvink N, Fiévez L, Dogné S, Bureau F, Art T, Lekeux P. Comparison of inulin with urea as dilutional markers of bronchoalveolar lavage in healthy and heaves-affected horses.. Vet Res 2001 Mar-Apr;32(2):145-54.
- Gerber V, Baleri D, Klukowska-Rötzler J, Swinburne JE, Dolf G. Mixed inheritance of equine recurrent airway obstruction.. J Vet Intern Med 2009 May-Jun;23(3):626-30.
- Gibeon D, Chung KF. The investigation of severe asthma to define phenotypes.. Clin Exp Allergy 2012 May;42(5):678-92.
Citations
This article has been cited 3 times.- Padoan E, Ferraresso S, Pegolo S, Barnini C, Castagnaro M, Bargelloni L. Gene Expression Profiles of the Immuno-Transcriptome in Equine Asthma. Animals (Basel) 2022 Dec 20;13(1).
- Gy C, Leclere M, Vargas A, Grimes C, Lavoie JP. Investigation of blood biomarkers for the diagnosis of mild to moderate asthma in horses. J Vet Intern Med 2019 Jul;33(4):1789-1795.
- Lendl L, Barton AK. Equine Asthma Diagnostics: Review of Influencing Factors and Difficulties in Diagnosing Subclinical Disease. Animals (Basel) 2024 Dec 4;14(23).
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