Selection of recently isolated colicinogenic Escherichia coli strains inhibitory to Escherichia coli O157:H7.
Abstract: Escherichia coli strains were screened for their ability to inhibit E. coli O157:H7. An initial evaluation of 18 strains carrying previously characterized colicins determined that only colicin E7 inhibited all of the E. coli O157:H7 strains tested. A total of 540 strains that had recently been isolated from humans and nine different animal species (cats, cattle, chickens, deer, dogs, ducks, horses, pigs, and sheep) were tested by a flip-plating technique. Approximately 38% of these strains were found to inhibit noncolicinogenic E. coli K12 strains. The percentage of potentially colicinogenic E. coli per animal species ranged from 14% for horse isolates to 64% for sheep strains. Those isolates that inhibited E. coli K12 were screened against E. coli O157:H7, and 42 strains were found to be capable of inhibiting all 22 pathogenic strains tested. None of these 42 strains produced bacteriophages, and only 24 isolates inhibited serotype O157:H7 in liquid culture. The inhibitory activity of these strains was completely eliminated by treatment with proteinase K. When mixtures of these 24 colicinogenic strains were grown in anaerobic continuous culture, the four-strain E. coli O157:H7 population was reduced at a rate of 0.25 log10 cells per ml per h, which was fivefold faster than the washout rate. Two strains originally isolated from cat feces (F16) and human feces (H30) were identified by repetitive sequences polymerase chain reaction as the predominant isolates in continuous cultures. The results of this work indicate that animal species other than cattle can be sources of anti-O157 colicinogenic strains, and these results also lead to the identification of at least two isolates that could potentially be used in preharvest control strategies.
Publication Date: 2002-09-18 PubMed ID: 12233846DOI: 10.4315/0362-028x-65.9.1381Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research aims to identify Escherichia coli (E. coli) strains capable of inhibiting the growth of the harmful E. coli O157:H7 strain. It emphasizes the role of colicinogenic strains in targeting the harmful strain and concluded that animals other than cattle can possess anti-O157 colicinogenic strains, identifying two isolates for potential use in preharvest control strategies.
Methods and Materials
- The study began with screening 18 E. coli strains carrying previously characterized colicins for their ability to inhibit E. coli O157:H7. The researchers determined that only colicin E7 inhibited all tested E. coli O157:H7 strains.
- The researchers then tested a larger pool of 540 E. coli strains, recently isolated from humans and several animal species using a flip-plating technique. This technique involves inverting a plate to allow for counting colonies and detection of species able to inhibit the growth of the target species.
Results
- Outcomes from the large-scale testing revealed that approximately 38% of these strains could inhibit E. coli K12 strains which are noncolicinogenic. Variation existed between the different hosts, with horse isolates exhibiting only 14% potential colicinogenicity while sheep strains exhibited a much higher rate at 64%.
- These restrictive strains were further tested against E. coli O157:H7, with 42 successfully inhibiting all 22 pathogenic strains tested. Out of these 42 strains, 24 demonstrated inhibitory activity in liquid culture, which was eliminated entirely by treatment with proteinase K, indicating the protein-based nature of the inhibitory mechanism.
- The researchers explored the impact of colicinogenic strains on the O157:H7 population rate, combining these strains in anaerobic continuous culture. The resultant population decrease of O157:H7 was far quicker than the washout rate, reflecting the potential application for control strategies using these strains.
Conclusions and Implications
- Two isolates, F16 and H30, originally sourced from cat and human feces respectively, were identified as the principal isolates in continuous cultures via repetitive sequences polymerase chain reaction method.
- The study concludes that animal species other than cattle can serve as sources of anti-O157 colicinogenic strains. It also highlights the potential of at least two identified isolates that could be used strategically in preharvest control to contain the spread of harmful E. coli O157:H7.
Cite This Article
APA
Schamberger GP, Diez-Gonzalez F.
(2002).
Selection of recently isolated colicinogenic Escherichia coli strains inhibitory to Escherichia coli O157:H7.
J Food Prot, 65(9), 1381-1387.
https://doi.org/10.4315/0362-028x-65.9.1381 Publication
Researcher Affiliations
- Department of Food Science and Nutrition, University of Minnesota, St. Paul 55108, USA.
MeSH Terms
- Animals
- Animals, Domestic
- Colicins / biosynthesis
- Colony Count, Microbial
- Disease Reservoirs
- Endopeptidase K / metabolism
- Escherichia coli / metabolism
- Escherichia coli / physiology
- Escherichia coli O157 / growth & development
- Food Microbiology
- Humans
- Oxygen
- Species Specificity
- Time Factors
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