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Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc1998; 10(3); 229-236; doi: 10.1177/104063879801000302

Serologic response of horses to the structural proteins of equine arteritis virus.

Abstract: Equine arteritis virus (EAV) is the causative agent of equine viral arteritis, an apparently emerging disease of equids. In this study, the antibody response of horses to the structural proteins of EAV was evaluated using gradient-purified EAV virions and baculovirus-expressed recombinant EAV structural proteins (G(L), G(S), M, N) as antigens in a Western immunoblotting assay. Thirty-three sera from horses that previously had been naturally or experimentally infected with EAV were evaluated, including samples from mares, geldings, and both persistently and nonpersistently infected stallions. Sera also were evaluated from 4 horses that had been vaccinated with the commercial modified live EAV vaccine. The data suggest that the serologic response of individual horses to EAV may vary with the infecting virus strain and duration of infection. The M protein was most consistently recognized by the various serum samples, whereas the response to the N and G(L) proteins was variable and the G(S) protein was bound by only 1 serum sample. The immunoblotting assay definitively established the protein specificity of the humoral response of horses to EAV; however, it clearly is less sensitive than the standard serum neutralization (SN) test--2 of the 37 sera that were seropositive by the SN test failed to react in the immunoblot assay with any EAV structural protein. Furthermore, the G(L) protein expresses the known neutralization determinants of EAV, yet only 22 of the 37 sera that had SN antibodies bound the G(L) protein in the immunoblotting assay. Information from this study will assist ongoing efforts to develop improved methods for the serologic diagnosis of EAV infection of horses.
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Publication Date: 1998-07-31 PubMed ID: 9683071DOI: 10.1177/104063879801000302Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't
  • Research Support
  • U.S. Gov't
  • Non-P.H.S.

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research paper investigates the antibody response of horses to the structural proteins of Equine arteritis virus (EAV), a disease-causing agent in horses. Through the use of specific testing, the paper suggests that response to the virus may vary dependent on the strain and length of infection, and that the available diagnostic methods may not be fully sensitive.

Research Methodology and Findings

  • The study used gradient-purified EAV virions and baculovirus-expressed recombinant EAV structural proteins as antigens in a Western immunoblotting assay to examine the antibody response of horses.
  • The researchers used sera from 33 horses previously infected either naturally or experimentally with EAV including mares, geldings, and both persistently and nonpersistently infected stallions. In addition, 4 horses vaccinated with a commercial EAV vaccine were also investigated.
  • The data indicated that a horse’s serologic response to EAV might vary depending on the virus strain and the length of the infection.
  • The structural protein M was most consistently recognized by the horse serum samples, while the responses to the N and GL proteins varied. Only one sample was bound by the GS protein.
  • While the immunoblotting assay confirmed the protein specificity of horses’ humoral response to EAV, it was found to be less sensitive than the standard serum neutralization (SN) test.
  • Two of the 37 sera samples that were seropositive through the SN test failed to react on the immunoblot assay with any EAV structural protein. Despite the GL protein expressing EAV neutralization determinants, only 22 out of the 37 sera with SN antibodies bound the GL protein in the assay.

Conclusion and Significance

  • The research’s findings will contribute towards bettering the understanding of horses’ antibody response to EAV, especially in relation to the response to different virus strains and infection durations.
  • The limitations observed with the immunoblotting assay compared to the standard SN test shed light on potential areas of improvement for diagnostic methods.
  • Ultimately, the knowledge from this study will aid in the development of improved methods for serologic diagnosis of EAV infection in horses, leading to better disease control and prevention strategies.

Cite This Article

APA
MacLachlan NJ, Balasuriya UB, Hedges JF, Schweidler TM, McCollum WH, Timoney PJ, Hullinger PJ, Patton JF. (1998). Serologic response of horses to the structural proteins of equine arteritis virus. J Vet Diagn Invest, 10(3), 229-236. https://doi.org/10.1177/104063879801000302

Publication

Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
ISSN: 1040-6387
NlmUniqueID: 9011490
Country: United States
Language: English
Volume: 10
Issue: 3
Pages: 229-236

Researcher Affiliations

MacLachlan, N J
  • Department of Pathology, Microbiology, and Immunology, School of Veterinary Medicine, University of California, Davis 95616, USA.
Balasuriya, U B
    Hedges, J F
      Schweidler, T M
        McCollum, W H
          Timoney, P J
            Hullinger, P J
              Patton, J F

                MeSH Terms

                • Animals
                • Antibodies, Viral / blood
                • Antibody Formation
                • Antigens, Viral / genetics
                • Antigens, Viral / immunology
                • Arterivirus Infections / immunology
                • Arterivirus Infections / prevention & control
                • Arterivirus Infections / veterinary
                • DNA Primers
                • Equartevirus / genetics
                • Equartevirus / immunology
                • Horse Diseases / immunology
                • Horse Diseases / prevention & control
                • Horses
                • Open Reading Frames
                • Polymerase Chain Reaction
                • Recombinant Proteins / immunology
                • Viral Structural Proteins / genetics
                • Viral Structural Proteins / immunology
                • Viral Vaccines
                • Virion / immunology

                Citations

                This article has been cited 21 times.
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