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Home / Equine Research Bank / Mem Inst Oswaldo Cruz / Serological detection of West Nile virus in horses and chick...
Memorias do Instituto Oswaldo Cruz2013; 107(8); 1073-1075; doi: 10.1590/s0074-02762012000800020

Serological detection of West Nile virus in horses and chicken from Pantanal, Brazil.

Abstract: In an effort to detect West Nile virus (WNV) in Brazil, we sampled serum from horses and chickens from the Pantanal region of the state of Mato Grosso and tested for flavivirus-reactive antibodies by blocking ELISA. The positive samples were further confirmed for serological evidence of WNV infection in three (8%) of the 38 horses and one (3.2%) of the 31 chickens using an 80% plaque-reduction neutralisation test (PRNT80). These results provide evidence of the circulation of WNV in chickens and horses in Pantanal.
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Publication Date: 2013-01-09 PubMed ID: 23295763PubMed Central: PMC4782916DOI: 10.1590/s0074-02762012000800020Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigates the presence of the West Nile Virus (WNV) in horses and chickens from the Pantanal region, Brazil by using a blocking ELISA test. It finds evidence that the WNV is circulating in these species to a certain extent.

Objective of Research

  • The main objective of the study was to detect the presence of West Nile Virus (WNV) in chickens and horses in the Pantanal region of Brazil.

Methodology

  • The researchers sampled serum from horses and chickens from the region. This serum is the fluid part of blood that doesn’t include blood cells, platelets, or fibrinogens and is often used in testing for the presence of viruses.
  • These samples were then tested for the presence of flavivirus-reactive antibodies by a process known as a blocking ELISA or Enzyme-Linked Immunosorbent Assay. ELISA is a commonly used laboratory test to detect and measure antibodies in the blood. It can be used to diagnose various diseases, including those caused by viruses such as WNV.
  • The positive samples that were found to contain flavivirus-reactive antibodies were then further tested for serological evidence of a WNV infection. This was done using an 80% plaque-reduction neutralisation test (PRNT80). A PRNT is a type of virus neutralisation test that measures the ability of serum from an infected individual to neutralise virus infection in cell culture.

Results and Conclusion

  • The results of the research indicated that three (or 8%) of the 38 sampled horses and one (or 3.2%) of the 31 sampled chickens showed serological evidence of a WNV infection as confirmed by the PRNT80 test.
  • This suggests that the WNV is indeed circulating among horses and chickens in the Pantanal region of Brazil. The research offers important insights into the spread of West Nile Virus in that specific region.

Cite This Article

APA
Melandri V, Guimarães AÉ, Komar N, Nogueira ML, Mondini A, Fernandez-Sesma A, Alencar J, Bosch I. (2013). Serological detection of West Nile virus in horses and chicken from Pantanal, Brazil. Mem Inst Oswaldo Cruz, 107(8), 1073-1075. https://doi.org/10.1590/s0074-02762012000800020

Publication

Memorias do Instituto Oswaldo Cruz
ISSN: 1678-8060
NlmUniqueID: 7502619
Country: Brazil
Language: English
Volume: 107
Issue: 8
Pages: 1073-1075
PII: S0074-02762012000800020

Researcher Affiliations

Melandri, Vanessa
  • Laboratório de Diptera, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, RJ, Brasil. melandri@ioc.fiocruz.br
Guimarães, Anthony Érico
    Komar, Nicholas
      Nogueira, Maurício L
        Mondini, Adriano
          Fernandez-Sesma, Ana
            Alencar, Jeronimo
              Bosch, Irene

                MeSH Terms

                • Animals
                • Antibodies, Viral / blood
                • Bird Diseases / diagnosis
                • Bird Diseases / epidemiology
                • Brazil / epidemiology
                • Chickens
                • Enzyme-Linked Immunosorbent Assay / veterinary
                • Horse Diseases / diagnosis
                • Horse Diseases / epidemiology
                • Horses
                • Neutralization Tests
                • Seroepidemiologic Studies
                • West Nile Fever / diagnosis
                • West Nile Fever / epidemiology
                • West Nile Fever / veterinary
                • West Nile virus / immunology

                Grant Funding

                • CC999999 / Intramural CDC HHS

                References

                This article includes 13 references
                1. Beaty BJ, Calisher CH, Shope RE. Diagnostic procedures for viral, rickettsial and chlamydial infections. .
                2. Blitvich BJ, Bowen RA, Marlenee NL, Hall RA, Bunning ML, Beaty BJ. Epitope-blocking enzyme-linked immunosorbent assays for detection of west nile virus antibodies in domestic mammals.. J Clin Microbiol 2003 Jun;41(6):2676-9.
                  pmc: PMC156482pubmed: 12791902doi: 10.1128/jcm.41.6.2676-2679.2003google scholar: lookup
                3. Bosch I, Herrera F, Navarro JC, Lentino M, Dupuis A, Maffei J, Jones M, Fernández E, Pérez N, Pérez-Emán J, Guimarães AE, Barrera R, Valero N, Ruiz J, Velásquez G, Martinez J, Comach G, Komar N, Spielman A, Kramer L. West Nile virus, Venezuela.. Emerg Infect Dis 2007 Apr;13(4):651-3.
                  pmc: PMC2725982pubmed: 17561567doi: 10.3201/eid1304.061383google scholar: lookup
                4. Adrián Diaz L, Komar N, Visintin A, Dantur Juri MJ, Stein M, Lobo Allende R, Spinsanti L, Konigheim B, Aguilar J, Laurito M, Almirón W, Contigiani M. West Nile virus in birds, Argentina.. Emerg Infect Dis 2008 Apr;14(4):689-91.
                  pmc: PMC2570931pubmed: 18394305doi: 10.3201/eid1404.071257google scholar: lookup
                5. Komar N, Clark GG. West Nile virus activity in Latin America and the Caribbean.. Rev Panam Salud Publica 2006 Feb;19(2):112-7.
                  pubmed: 16551385doi: 10.1590/s1020-49892006000200006google scholar: lookup
                6. Mattar S, Edwards E, Laguado J, González M, Alvarez J, Komar N. West Nile virus antibodies in Colombian horses.. Emerg Infect Dis 2005 Sep;11(9):1497-8.
                  pmc: PMC3310636pubmed: 16673523doi: 10.3201/eid1109.050426google scholar: lookup
                7. Mattar S, Komar N, Young G, Alvarez J, Gonzalez M. Seroconversion for West Nile and St. Louis encephalitis viruses among sentinel horses in Colombia.. Mem Inst Oswaldo Cruz 2011 Dec;106(8):976-9.
                  pubmed: 22241119doi: 10.1590/s0074-02762011000800012google scholar: lookup
                8. MS/SVS - Ministério da Saúde/Secretaria de Vigilância em Saúde Brasil. Primeiro inquérito sorológico em aves migratórias e nativas do Parque Nacional da Lagoa do Peixe/RS para detecção do vírus do Nilo ocidental. Boletim eletrônico epidemiológico 2003;3:3–12.
                9. MS/SVS - Ministério da Saúde/Secretaria de Vigilância em Saúde Brasil. Inquérito sorológico em aves migratórias e residentes de Galinhos/RN para detecção do vírus da febre do Nilo ocidental e outros vírus. Boletim eletrônico epidemiológico 2004;4:1–12.
                10. Pauvolid-Corrêa A, Morales MA, Levis S, Figueiredo LT, Couto-Lima D, Campos Z, Nogueira MF, da Silva EE, Nogueira RM, Schatzmayr HG. Neutralising antibodies for West Nile virus in horses from Brazilian Pantanal.. Mem Inst Oswaldo Cruz 2011 Jun;106(4):467-74.
                  pubmed: 21739036doi: 10.1590/s0074-02762011000400014google scholar: lookup
                11. Soares CN, Castro MJ, Peralta JM, Freitas MR, Puccioni-Sohler M. Is West Nile virus a potential cause of central nervous system infection in Brazil?. Arq Neuropsiquiatr 2010 Oct;68(5):761-3.
                  pubmed: 21049189doi: 10.1590/s0004-282x2010000500016google scholar: lookup
                12. Tauro L, Marino B, Diaz LA, Lucca E, Gallozo D, Spinsanti L, Contigiani M. Serological detection of St. Louis encephalitis virus and West Nile virus in equines from Santa Fe, Argentina.. Mem Inst Oswaldo Cruz 2012 Jun;107(4):553-6.
                  pubmed: 22666870doi: 10.1590/s0074-02762012000400019google scholar: lookup
                13. Turell MJ, Dohm DJ, Sardelis MR, Oguinn ML, Andreadis TG, Blow JA. An update on the potential of north American mosquitoes (Diptera: Culicidae) to transmit West Nile Virus.. J Med Entomol 2005 Jan;42(1):57-62.
                  pubmed: 15691009doi: 10.1093/jmedent/42.1.57google scholar: lookup

                Citations

                This article has been cited 21 times.
                1. Wanzeller ALM, da Silva FS, Hernández LHA, Barros LJL, Freitas MNO, Santos MM, Gonçalves EJ, Pantoja JAS, Lima CS, Lima MF, Costa LRO, das Chagas LL, Silva IF, da Cunha TCADS, do Nascimento BLS, Vasconcelos HB, da Rosa EST, Rodrigues SG, Azevedo RDSDS, Martins LC, Casseb LMN, Chiang JO, Nunes Neto JP, Cruz ACR, Carvalho VL, Vasconcelos PFDC, da Silva EVP. Isolation of Flaviviruses and Alphaviruses with Encephalitogenic Potential Diagnosed by Evandro Chagas Institute (Pará, Brazil) in the Period of 1954-2022: Six Decades of Discoveries. Viruses 2023 Apr 10;15(4).
                  doi: 10.3390/v15040935pubmed: 37112917google scholar: lookup
                2. Neto JPN, Reis LAM, Freitas MNO, do Nascimento BLS, das Chagas LL, da Costa HHM, Rodrigues JCP, Braga CM, da Silva EVP, Silva SP, Martins LC. First Isolation and Genome Sequence Analysis of West Nile Virus in Mosquitoes in Brazil. Trop Med Infect Dis 2023 Apr 20;8(4).
                  doi: 10.3390/tropicalmed8040237pubmed: 37104362google scholar: lookup
                3. Chalhoub FLL, Horta MAP, Alcantara LCJ, Morales A, Dos Santos LMB, Guerra-Campos V, Rodrigues CDS, Santos CC, Mares-Guia MAM, Pauvolid-Corrêa A, de Filippis AMB. Serological Evidence of Exposure to Saint Louis Encephalitis and West Nile Viruses in Horses of Rio de Janeiro, Brazil. Viruses 2022 Nov 6;14(11).
                  doi: 10.3390/v14112459pubmed: 36366557google scholar: lookup
                4. Fritsch H, Pereira FM, Costa EA, Fonseca V, Tosta S, Xavier J, Levy F, Oliveira C, Menezes G, Lima J, Santos L, Silva L, Nardy V, Astete MKG, Santos BSÁDS, Aguiar NR, Guedes MIMC, Faria GC, Furtini R, Drumond SRM, Cunha GM, Souza MSPL, Jesus R, Guimarães SAF, Nuno IC, Santana ICB, Sá JEU, Santos GR, Silva WS, Guedes TF, Araújo ELL, Said RFDC, Albuquerque CFC, Peterka CRL, Romano APM, Cunha RVD, Filippis AMB, Leal E Silva de Mello A, Giovanetti M, Alcantara LCJ. Retrospective Investigation in Horses with Encephalitis Reveals Unnoticed Circulation of West Nile Virus in Brazil. Viruses 2022 Jul 14;14(7).
                  doi: 10.3390/v14071540pubmed: 35891521google scholar: lookup
                5. de Figueiredo MLG, Williams EP, Jonsson CB, Khan MJ, Nunes MRT, de Lima CPS, Figueiredo LTM, Costa MRF, Mourão MPG, Lacerda MVG, Aquino VH. Screening of febrile patients with suspected malaria from the Brazilian Amazon for virus infection. Arch Virol 2022 Nov;167(11):2151-2162.
                  doi: 10.1007/s00705-022-05514-7pubmed: 35841448google scholar: lookup
                6. Löwen Levy Chalhoub F, Maia de Queiroz-Júnior E, Holanda Duarte B, Eielson Pinheiro de Sá M, Cerqueira Lima P, Carneiro de Oliveira A, Medeiros Neves Casseb L, Leal das Chagas L, Antônio de Oliveira Monteiro H, Sebastião Alberto Santos Neves M, Facundo Chaves C, Jean da Silva Moura P, Machado Rapello do Nascimento A, Giesbrecht Pinheiro R, Roberio Soares Vieira A, Bergson Pinheiro Moura F, Osvaldo Rodrigues da Silva L, Nogueira Farias da Escóssia K, Caranha de Sousa L, Leticia Cavalcante Ramalho I, Williams Lopes da Silva A, Maria Simōes Mello L, Felix de Souza F, das Chagas Almeida F, Dos Santos Rodrigues R, do Vale Chagas D, Ferreira-de-Brito A, Ribeiro Leite Jardim Cavalcante K, Angélica Monteiro de Mello Mares-Guia M, Martins Guerra Campos V, Rodrigues da Costa Faria N, Adriano da Cunha E Silva Vieira M, Cesar Lima de Mendonça M, Camila Amorim de Alvarenga Pivisan N, de Oliveira Moreno J, Aldessandra Diniz Vieira M, Gonçalves de Aguiar Gomes R, Montenegro de Carvalho Araújo F, Henrique de Oliveira Passos P, Garkauskas Ramos D, Pecego Martins Romano A, Carício Martins L, Lourenço-de-Oliveira R, Maria Bispo de Filippis A, Pauvolid-Corrêa A. West Nile Virus in the State of Ceará, Northeast Brazil. Microorganisms 2021 Aug 10;9(8).
                  doi: 10.3390/microorganisms9081699pubmed: 34442778google scholar: lookup
                7. Costa ÉA, Giovanetti M, Silva Catenacci L, Fonseca V, Aburjaile FF, Chalhoub FLL, Xavier J, Campos de Melo Iani F, da Cunha E Silva Vieira MA, Freitas Henriques D, Medeiros DBA, Guedes MIMC, Senra Álvares da Silva Santos B, Gonçalves Silva AS, de Pino Albuquerque Maranhão R, da Costa Faria NR, Farinelli de Siqueira R, de Oliveira T, Ribeiro Leite Jardim Cavalcante K, Oliveira de Moura NF, Pecego Martins Romano A, Campelo de Albuquerque CF, Soares Feitosa LC, Martins Bayeux JJ, Bertoni Cavalcanti Teixeira R, Lisboa Lobato O, da Costa Silva S, Bispo de Filippis AM, Venâncio da Cunha R, Lourenço J, Alcantara LCJ. West Nile Virus in Brazil. Pathogens 2021 Jul 15;10(7).
                  doi: 10.3390/pathogens10070896pubmed: 34358046google scholar: lookup
                8. Weber MN, Mosena ACS, Baumbach LF, da Silva MS, Canova R, Dos Santos DRL, Budaszewski RDF, de Oliveira LV, Soane MM, Saraiva NB, Bellucco FT, Mazurek BA, Diehl GN, Gil LHVG, Borba MR, Corbellini LG, Canal CW. Serologic evidence of West Nile virus and Saint Louis encephalitis virus in horses from Southern Brazil. Braz J Microbiol 2021 Jun;52(2):1021-1027.
                  doi: 10.1007/s42770-021-00474-7pubmed: 33797731google scholar: lookup
                9. Morel AP, Webster A, Zitelli LC, Umeno K, Souza UA, Prusch F, Anicet M, Marsicano G, Bandarra P, Trainini G, Stocker J, Giani D, Fortes FB, Goenaga S, Reck J. Serosurvey of West Nile virus (WNV) in free-ranging raptors from Brazil. Braz J Microbiol 2021 Mar;52(1):411-418.
                  doi: 10.1007/s42770-020-00393-zpubmed: 33108590google scholar: lookup
                10. Martins LC, Silva EVPD, Casseb LMN, Silva SPD, Cruz ACR, Pantoja JAS, Medeiros DBA, Martins Filho AJ, Cruz EDRMD, Araújo MTF, Cardoso JF, Cunha MACRD, Almada GL, Romano APM, Santos MGDP, Rodrigues GAP, Chiang JO, Quaresma JAS, Carvalho VL, Vasconcelos PFDC. First isolation of West Nile virus in Brazil. Mem Inst Oswaldo Cruz 2019 Jan 17;114:e180332.
                  doi: 10.1590/0074-02760180332pubmed: 30672980google scholar: lookup
                11. Ferreira FC Jr, Rodrigues RA, Sato Y, Borges MA, Braga ÉM. Searching for putative avian malaria vectors in a Seasonally Dry Tropical Forest in Brazil. Parasit Vectors 2016 Nov 16;9(1):587.
                  doi: 10.1186/s13071-016-1865-ypubmed: 27852326google scholar: lookup
                12. Vieira MA, Aguiar Ade A, Borba Ade S, Guimarães HC, Eulálio KD, de Albuquerque-Neto LL, Salmito Mdo A, Lima OB. WEST NILE FEVER IN BRAZIL: SPORADIC CASE, SILENT ENDEMIC DISEASE OR EPIDEMIC IN ITS INITIAL STAGES?. Rev Inst Med Trop Sao Paulo 2015 May-Jun;57(3):276.
                  doi: 10.1590/S0036-46652015000300017pubmed: 26200972google scholar: lookup
                13. Vieira MA, Romano AP, Borba AS, Silva EV, Chiang JO, Eulálio KD, Azevedo RS, Rodrigues SG, Almeida-Neto WS, Vasconcelos PF. West Nile Virus Encephalitis: The First Human Case Recorded in Brazil. Am J Trop Med Hyg 2015 Aug;93(2):377-9.
                  doi: 10.4269/ajtmh.15-0170pubmed: 26055749google scholar: lookup
                14. Chancey C, Grinev A, Volkova E, Rios M. The global ecology and epidemiology of West Nile virus. Biomed Res Int 2015;2015:376230.
                  doi: 10.1155/2015/376230pubmed: 25866777google scholar: lookup
                15. Degener CM, Ázara TM, Roque RA, Codeço CT, Nobre AA, Ohly JJ, Geier M, Eiras ÁE. Temporal abundance of Aedes aegypti in Manaus, Brazil, measured by two trap types for adult mosquitoes. Mem Inst Oswaldo Cruz 2014 Dec;109(8):1030-40.
                  doi: 10.1590/0074-0276140234pubmed: 25494470google scholar: lookup
                16. Elizondo-Quiroga D, Elizondo-Quiroga A. West nile virus and its theories, a big puzzle in Mexico and latin america. J Glob Infect Dis 2013 Oct;5(4):168-75.
                  doi: 10.4103/0974-777X.122014pubmed: 24672180google scholar: lookup
                17. Pauvolid-Corrêa A, Campos Z, Juliano R, Velez J, Nogueira RM, Komar N. Serological evidence of widespread circulation of West Nile virus and other flaviviruses in equines of the Pantanal, Brazil. PLoS Negl Trop Dis 2014 Feb;8(2):e2706.
                  doi: 10.1371/journal.pntd.0002706pubmed: 24551266google scholar: lookup
                18. Silva JR, Medeiros LC, Reis VP, Chavez JH, Munhoz TD, Borges GP, Soares OA, Campos CH, Machado RZ, Baldani CD, Silva ML, Faria JL, Silva EE, Figueiredo LT. Serologic survey of West Nile virus in horses from Central-West, Northeast and Southeast Brazil. Mem Inst Oswaldo Cruz 2013 Nov;108(7):921-3.
                  doi: 10.1590/S0074-02762013005000001pubmed: 24037110google scholar: lookup
                19. Pauvolid-Corrêa A, Kenney JL, Couto-Lima D, Campos ZM, Schatzmayr HG, Nogueira RM, Brault AC, Komar N. Ilheus virus isolation in the Pantanal, west-central Brazil. PLoS Negl Trop Dis 2013;7(7):e2318.
                  doi: 10.1371/journal.pntd.0002318pubmed: 23875051google scholar: lookup
                20. Lobato OL, Nogueira TDS, Lima TET, Andrade FJDC, de Macedo MGG, Pereira RS, Xavier J, Amorim MR, Barbosa PP, da Rocha AS, Silva SDC, Alcantara LCJ, de Souza WM, Proenca-Modena JL, Costa ÉA, Lima Neto AS, Feitosa LCS, Pires E Cruz MDS, Silva SMMS, Baêta SAF, Vieira MADCES, Deem SL, Catenacci LS. Orthoflavivirus nilense surveillance in the State of Piauí, northeastern Brazil. Mem Inst Oswaldo Cruz 2025;120:e240218.
                  doi: 10.1590/0074-027602402180pubmed: 40638500google scholar: lookup
                21. Dye-Braumuller KC, Prisco RA, Nolan MS. (Re)Emerging Arboviruses of Public Health Significance in the Brazilian Amazon. Microorganisms 2025 Mar 12;13(3).
                  doi: 10.3390/microorganisms13030650pubmed: 40142542google scholar: lookup
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