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Animals : an open access journal from MDPI2023; 13(13); doi: 10.3390/ani13132056

Serological Examinations of Significant Viral Infections in Domestic Donkeys at the Special Nature Reserve “Zasavica”, Serbia.

Abstract: The paper presents the findings of specific antibodies in the blood sera of donkeys against the following viruses: equine infectious anemia virus (EIAV), African horse sickness virus (AHSV), equine herpesvirus type 1 (EHV-1), equine influenza virus subtype H3N8 (EIV) and equine arteritis virus (EAV). The analyses were conducted during the year 2022. From a total of 199 donkeys bred in "Zasavica", blood was sampled from 53 animals (2 male donkeys and 51 female donkeys), aged 3 to 10 years. Specific antibodies against EIAV were not detected in any of the tested animals using the agar-gel immunodiffusion (AGID) assay. No specific antibodies against AHSV, tested by enzyme-linked immunosorbent assay (ELISA), or antibodies against EAV, tested by the virus neutralization test (VNT) and ELISA were detected in any of these animals. A positive serological result for EHV-1 was determined by the VNT in all animals, with antibody titer values ranging from 1:2 to 1:128, while a very low antibody titer value for EIV (subtype H3N8) of 1:16 was determined in 18 donkeys using the hemagglutination inhibition test (HI test).
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Publication Date: 2023-06-21 PubMed ID: 37443854PubMed Central: PMC10340027DOI: 10.3390/ani13132056Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study undertakes a comprehensive examination of various viral infections in a population of donkeys in Serbia’s “Zasavica” special nature reserve. The key findings underscore the presence of antibodies for equine herpesvirus type 1 (EHV-1) and equine influenza virus subtype H3N8 (EIV), while other tested viruses were not detected.

Samples and Methodology

  • The study involved 53 donkeys – 2 males and 51 females – aged between 3 to 10 years, sampled from a population of 199 animals.
  • Blood samples were taken from these animals in 2022 and examined for antibodies against several viruses including equine infectious anemia virus (EIAV), African horse sickness virus (AHSV), EHV-1, EIV, and equine arteritis virus (EAV).
  • Various assays such as agar-gel immunodiffusion (AGID), enzyme-linked immunosorbent assay (ELISA), virus neutralization test (VNT) and hemagglutination inhibition test (HI test) were used to conduct these investigations.

Findings

  • No antibodies against EIAV were found in any of the tested animals, as indicated by the AGID assay.
  • Similarly, the ELISA test did not detect the presence of antibodies against AHSV or EAV in any of the sampled donkeys.
  • All animals tested positive for EHV-1 based on the VNT, with antibody titer values ranging from 1:2 to 1:128.
  • Finally, the HI test revealed a low antibody titer value (1:16) against EIV (subtype H3N8) in 18 of the donkeys.

Implications

  • The results indicate that the donkey population in the reserve may have been exposed to EHV-1 and EIV, as reflected by the presence of respective antibodies in their blood samples.
  • The absence of antibodies for EIAV, AHSV, and EAV suggests these particular viral infections may not be prevalent among these donkeys.
  • This study contributes to the understanding of potential health risks and viral infections among donkeys in the “Zasavica” reserve.
  • However, more research is needed to further assess the significance of these findings and to develop appropriate preventative measures.

Cite This Article

APA
Lazić S, Savić S, Petrović T, Lazić G, Žekić M, Drobnjak D, Lupulović D. (2023). Serological Examinations of Significant Viral Infections in Domestic Donkeys at the Special Nature Reserve “Zasavica”, Serbia. Animals (Basel), 13(13). https://doi.org/10.3390/ani13132056

Publication

Animals : an open access journal from MDPI
ISSN: 2076-2615
NlmUniqueID: 101635614
Country: Switzerland
Language: English
Volume: 13
Issue: 13

Researcher Affiliations

Lazić, Sava
  • Scientific Veterinary Institute "Novi Sad", Rumenački put 20, 21000 Novi Sad, Serbia.
Savić, Sara
  • Scientific Veterinary Institute "Novi Sad", Rumenački put 20, 21000 Novi Sad, Serbia.
Petrović, Tamaš
  • Scientific Veterinary Institute "Novi Sad", Rumenački put 20, 21000 Novi Sad, Serbia.
Lazić, Gospava
  • Scientific Veterinary Institute "Novi Sad", Rumenački put 20, 21000 Novi Sad, Serbia.
Žekić, Marina
  • Scientific Veterinary Institute "Novi Sad", Rumenački put 20, 21000 Novi Sad, Serbia.
Drobnjak, Darko
  • Center for Preservation of Indigenous Breeds-CEPIB, Vere Dimitrijević, 11186 Zemun, Serbia.
Lupulović, Diana
  • Scientific Veterinary Institute "Novi Sad", Rumenački put 20, 21000 Novi Sad, Serbia.

Grant Funding

  • 680-00-00042/1/202 / Ministry of Agriculture, Forestry and Water Management of Republic of Serbia, Directorate for Agricultural Payments

Conflict of Interest Statement

The authors declare no conflict of interest.

References

This article includes 75 references
  1. Rossel S, Marshall F, Peters J, Pilgram T, Adams M.D, O’Connor D. Domestication of the donkey: Timing, processes, and indicators. Proc. Natl. Acad. Sci. USA 2008;105:3715–3720.
    doi: 10.1073/pnas.0709692105pmc: PMC2268817pubmed: 18332433google scholar: lookup
  2. Burden F, Thiemann A. Donkeys Are Different. J. Equine Vet. Sci. 2015;35:376–382.
    doi: 10.1016/j.jevs.2015.03.005google scholar: lookup
  3. Maggs H.C, Ainslie A, Bennett R.M. Donkey Ownership Provides a Range of Income Benefits to the Livelihoods of Rural Households in Northern Ghana. Animals 2021;11:3154.
    doi: 10.3390/ani11113154pmc: PMC8614285pubmed: 34827884google scholar: lookup
  4. Živkov Baloš M, Pelić D.L, Jakšić S, Lazić S. Donkey Milk: An Overview of its Chemical Composition and Main Nutritional Properties or Human Health Benefit Properties. J. Equine Vet. Sci. 2023;121:104225.
    doi: 10.1016/j.jevs.2023.104225pubmed: 36649829google scholar: lookup
  5. Norris S.L, Little H.A, Ryding J, Raw Z. Global donkey and mule populations: Figures and trends. PLoS ONE 2021;16:e0247830.
    doi: 10.1371/journal.pone.0247830pmc: PMC7906361pubmed: 33630957google scholar: lookup
  6. FAOSTAT Food and Agriculture Organization of the United Nations. [(accessed on 9 February 2023)]. Available online: https://www.fao.org/faostat/en/#data/QCL.
  7. Statistical Office of the Republic of Serbia Statistical Yearbook of the Republic of Serbia 2022. [(accessed on 15 June 2023)]; Available online: https://publikacije.stat.gov.rs/G2022/PdfE/G20222055.pdf.
  8. Urosevic M, Drobnjak D, Ivanov S. Autohtone Rase Magaraca u Srbiji [Indigenous Breeds of Donkeys in Serbia]. Proceedings of the Protection of Agrobiodiversity and Preservation of Indigenous Breeds of Domestic Animals Dimitrovgrad, Serbia. 28–30 June 2019.
  9. World Organization for Animal Health (WOAH) [(accessed on 21 January 2023)]. Available online: https://www.woah.org/en/what-we-do/animal-health-and-welfare/animal-diseases/?_tax_animal=terrestrials%2Cequine.
  10. Trailović D, Trailović R, Urošević M, Trailović I. Uzgoj, Nega I Bolesti Magaraca [Breeding, Care and Diseases of Donkeys]. Centar za Očuvanje Autohtonih Rasa—COAR, Zemun Beograd, Serbia: 2021.
  11. Barrandeguy M.E, Carossino M. Infectious Diseases in Donkeys and Mules: An Overview and Update. J. Equine Vet. Sci. 2018;65:98–105.
    doi: 10.1016/j.jevs.2018.02.026google scholar: lookup
  12. Sellon D.C. Equine Infectious Anemia. Vet. Clin. N. Am. Equine Pract. 1993;9:321–336.
    doi: 10.1016/S0749-0739(17)30399-1pmc: PMC7126748pubmed: 8395326google scholar: lookup
  13. Foil L.D, Issel C.J. Transmission of Retroviruses by Arthropods. Annu. Rev. Entomol. 1991;36:355–381.
    doi: 10.1146/annurev.en.36.010191.002035pubmed: 1848744google scholar: lookup
  14. Mealey R. Equine Infectious Anemia. Equine Infectious Diseases Saunders; St. Louis, MO, USA: 2014. p. 232.e9.
  15. Kemen M.J, Coggins L. Equine infectious anemia: Transmission from infected mares to foals. J. Am. Vet. Med. Assoc. 1972;161:496–499.
    pubmed: 4626365
  16. World Organization for Animal Health (WOAH) Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, Chapter: 3.6.1 African Horse Sickness (Infection with African Horse Sickness Virus) [(accessed on 6 March 2023)]. Available online: https://www.woah.org/fileadmin/Home/eng/Health_standards/tahm/3.06.01_AHS.pdf.
  17. Henning M. Animal Diseases in South Africa. Central News Agency; Sandton, South Africa: 1956. African Horse Sickness, Perdesiekte, Pestis Equorum; pp. 785–808.
  18. Hamblin C, Salt J.S, Mellor P.S, Graham S.D, Smith P.R, Wohlsein P. African Horse Sickness. Springer; Vienna, Austria: 1998. Donkeys as reservoirs of African horse sickness virus; pp. 37–47.
    doi: 10.1007/978-3-7091-6823-3_5pubmed: 9785494google scholar: lookup
  19. Coetzer J, Guthrie A. African Horsesickness. Infectious Diseases of Livestock Oxford University Press; Cape Town, South Africa: 2005. pp. 1231–1246.
  20. Molini U, Zaccaria G, Kandiwa E, Mushonga B, Khaiseb S, Ntahonshikira C, Chiwome B, Baines I, Madzingira O, Savini G. Seroprevalence of African horse sickness in selected donkey populations in Namibia. Vet. World 2020;13:1005–1009.
    doi: 10.14202/vetworld.2020.1005-1009pmc: PMC7311865pubmed: 32636601google scholar: lookup
  21. Lubroth J. African Horse Sickness and the Epizootic in Spain 1987. Equine Pract. 1988;10:26–33.
  22. Castillo-Olivares J. African horse sickness in Thailand: Challenges of controlling an outbreak by vaccination. Equine Vet. J. 2021;53:9–14.
    doi: 10.1111/evj.13353pmc: PMC7821295pubmed: 33007121google scholar: lookup
  23. Gordon S.J.G, Bolwell C, Rogers C.W, Musuka G, Kelly P, Guthrie A, Mellor P.S, Hamblin C. The sero-prevalence and sero-incidence of African horse sickness and equine encephalosis in selected horse and donkey populations in Zimbabwe. Onderstepoort J. Vet. Res. 2017;84:a1445.
    doi: 10.4102/ojvr.v84i1.1445pmc: PMC6238669pubmed: 28582979google scholar: lookup
  24. Ndebé M.M.F, Mouiche M.M.M, Moffo F, Poueme R.N.S, Awah-Ndukum J. Seroprevalence and Risk Factors of African Horse Sickness in Three Agroecological Zones of Cameroon. Vet. Med. Int. 2022;2022:2457772.
    doi: 10.1155/2022/2457772pmc: PMC9124128pubmed: 35607421google scholar: lookup
  25. Câmara R.J.F, Bueno B.L, Resende C.F, Balasuriya U.B.R, Sakamoto S.M, dos Reis J.K.P. Viral Diseases that Affect Donkeys and Mules. Animals 2020;10:2203.
    doi: 10.3390/ani10122203pmc: PMC7760297pubmed: 33255568google scholar: lookup
  26. International Committee on Taxonomy of Viruses. [(accessed on 20 August 2022)]. Available online: https://ictv.global/taxonomy.
  27. Crabb B.S, Studdert M.J. Equine Herpesviruses 4 (Equine Rhinopneumonitis Virus) and 1 (Equine Abortion Virus). Adv. Virus Res. 1995;45:153–190.
    doi: 10.1016/s0065-3527(08)60060-3pubmed: 7793324google scholar: lookup
  28. Allen G. Respiratory Infections by Equine Herpesvirus Types 1 and 4. Equine Respiratory Diseases International Veterinary Information Service; Ithaca, NY, USA: 2002.
  29. Laval K, Poelaert K.C.K, Van Cleemput J, Zhao J, Vandekerckhove A.P, Gryspeerdt A.C, Garré B, van der Meulen K, Baghi H.B, Dubale H.N. The Pathogenesis and Immune Evasive Mechanisms of Equine Herpesvirus Type. Front. Microbiol. 2021;12:662686.
    doi: 10.3389/fmicb.2021.662686pmc: PMC7970122pubmed: 33746936google scholar: lookup
  30. World Organization for Animal Health (WOAH) Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, Chapter: 3.6.9 Equine Rhinopneumonitis (Infection with Equid Herpesvirus-1 and -4) [(accessed on 6 March 2023)]. Available online: https://www.woah.org/fileadmin/Home/eng/Health_standards/tahm/3.06.09_EQUINE_RHINO.pdf.
  31. Laval K, Favoreel H.W, Nauwynck H.J. Equine herpesvirus type 1 replication is delayed in CD172a+ monocytic cells and controlled by histone deacetylases. J. Gen. Virol. 2015;96:118–130.
    doi: 10.1099/vir.0.067363-0pubmed: 25239765google scholar: lookup
  32. Oladunni F.S, Horohov D.W, Chambers T.M. EHV-1: A Constant Threat to the Horse Industry. Front. Microbiol. 2019;10:2668.
    doi: 10.3389/fmicb.2019.02668pmc: PMC6901505pubmed: 31849857google scholar: lookup
  33. Ataseven V.S, Arslan H.H. Equine infectious anemia in mules, donkeys, and horses: Epidemiologic studies in the different geographic regions of Turkey. J. Equine Vet. Sci. 2005;25:439–441.
    doi: 10.1016/j.jevs.2005.09.007google scholar: lookup
  34. Yildirim Y, Yilmaz V, Kirmizigul A.H. Equine herpes virus type 1 (EHV-1) and 4 (EHV-4) infections in horses and donkeys in northeastern Turkey. Iran. J. Vet. Res. 2015;16:341–344.
    doi: 10.22099/IJVR.2015.3285pmc: PMC4782672pubmed: 27175200google scholar: lookup
  35. Getachew M, Alemayehu F, Chala C, Amare B, Kassa D, Burden F, Wernery R, Wernery U. A Cross-Sectional Sero-Survey of Some Infectious Diseases of Working Equids in Central Ethiopia. J. Vet. Med. Anim. Health. 2014;6:231–238.
  36. Mekonnen A, Eshetu A, Gizaw D. Equine herpesvirus 1 and/or 4 in working equids: Seroprevalence and risk factors in North Shewa Zone, Ethiopia. Ethiop. Vet. J. 2017;21:28–39.
    doi: 10.4314/evj.v21i2.3google scholar: lookup
  37. Moghazy M, Mohamed G.A, Faiysal I.H, Elsayed M.I, Safaa M.A.W. Seroprevalence of Equine Herpes Virus Type-1 in Horses and Donkeys in Qalubiah Governorate in Egypt. IJRDO-J. Biol. Sci. 2017;3:1–18.
  38. Azab W, Bedair S, AbdelGawad A, Eschke K, Farag G.K, Abdel-Raheim A, Greenwood A.D, Osterrieder N, Ali A.A.H. Detection of equid herpesviruses among different Arabian horse populations in Egypt. Vet. Med. Sci. 2019;5:361–371.
    doi: 10.1002/vms3.176pmc: PMC7155215pubmed: 31149784google scholar: lookup
  39. Wegdan H.A, Intisar K.S, Shaza M.M, Algezoli O.A, Ballal A, Ihsan H.A, Sahar M.E, Baraa A.M, Manal H.S, Muna E.A. Serological Detection of Equine Herpes Virus (EHV) Type 1 and 4 in Sudan. Microbiol. Res. J. Int. 2016;14:1–6.
    doi: 10.9734/BMRJ/2016/25803google scholar: lookup
  40. Lara M.d.C.C.d.S.H, Villalobos E.M.C, Cunha E.M.S, De Oliveira J.V, Nassar A.F.D.C, Silva L.M.P, Okuda L.H, Romaldini A.H.D.C.N, Marques E.C, Mori E. Occurrence of viral diseases in donkeys (Equus asinus) in São Paulo State, Brazil. Braz. J. Vet. Res. Anim. Sci. 2017;54:154–158.
    doi: 10.11606/issn.1678-4456.bjvras.2017.121241google scholar: lookup
  41. Goodrich E.L, McLean A, Guarino C. A Pilot Serosurvey for Selected Pathogens in Feral Donkeys (Equus asinus). Animals 2020;10:1796.
    doi: 10.3390/ani10101796pmc: PMC7599684pubmed: 33023217google scholar: lookup
  42. Seeber P.A, Quintard B, Sicks F, Dehnhard M, Greenwood A.D, Franz M. Environmental stressors may cause equine herpesvirus reactivation in captive Grévy’s zebras (Equus grevyi). PeerJ 2018;6:e5422.
    doi: 10.7717/peerj.5422pmc: PMC6109370pubmed: 30155350google scholar: lookup
  43. Guevara L, Abdelgawad A, Onzere C, Greenwood A.D, Davidson Z, Bishop R, Mutinda M. Seroprevalence of Equine Herpesviruses 1 and 9 (EHV-1 and EHV-9) in Wild Grévy’s Zebra (Equus grevyi) in Kenya. J. Wildl. Dis. 2018;54:848–851.
    doi: 10.7589/2018-01-003pubmed: 29792760google scholar: lookup
  44. AbdelGawad A, Hermes R, Damiani A, Lamglait B, Czirják G, East M, Aschenborn O, Wenker C, Kasem S, Osterrieder N. Comprehensive Serology Based on a Peptide ELISA to Assess the Prevalence of Closely Related Equine Herpesviruses in Zoo and Wild Animals. PLoS ONE 2015;10:e0138370.
    doi: 10.1371/journal.pone.0138370pmc: PMC4574707pubmed: 26378452google scholar: lookup
  45. Centers for Disease Control and Prevention (CDC): Influenza (Flu) [(accessed on 31 May 2023)]; Available online: https://www.cdc.gov/flu/about/viruses/change.htm.
  46. Sovinova O, Tumova B, Pouska F, Nemec J. Isolation of a virus causing respiratory disease in horses. Acta Virol. 1958;2:52–61.
    pubmed: 13533033
  47. Waddell G.H, Teigland M.B, Sigel M.M. A new influenza virus associated with equine respiratory disease. J. Am. Vet. Med. Assoc. 1963;143:587–590.
    pubmed: 14077956
  48. Badji A, Faye A, Thior Y.E.H, Sarr S, Mbengue B, Sene A. Risk factors for infection with equine influenza virus in donkeys (Equus asinus) in Senegal. Int. J. Biol. Chem. Sci. 2022;15:1783–1790.
    doi: 10.4314/ijbcs.v15i5.7google scholar: lookup
  49. Boone S.A, Gerba C.P. The occurrence of influenza A virus on household and day care center fomites. J. Infect. 2005;51:103–109.
    doi: 10.1016/j.jinf.2004.09.011pubmed: 16038759google scholar: lookup
  50. Waghmare S, Mode S, Kolte A, Babhulkar N, Vyavahare S, Patel A. Equine Influenza: An Overview. Vet. World 2010;3:194–197.
  51. Crawford P.C, Dubovi E.J, Castleman W.L, Stephenson I, Gibbs E.P.J, Chen L, Smith C, Hill R.C, Ferro P, Pompey J. Transmission of Equine Influenza Virus to Dogs. Science 2005;310:482–485.
    doi: 10.1126/science.1117950pubmed: 16186182google scholar: lookup
  52. Guo Y, Wang M, Kawaoka Y, Gorman O, Ito T, Saito T, Webster R.G. Characterization of a new avian-like influenza A virus from horses in China. Virology 1992;188:245–255.
    doi: 10.1016/0042-6822(92)90754-Dpubmed: 1314452google scholar: lookup
  53. Xie T, Anderson B.D, Daramragchaa U, Chuluunbaatar M, Gray G.C. A Review of Evidence that Equine Influenza Viruses Are Zoonotic. Pathogens 2016;5:50.
    doi: 10.3390/pathogens5030050pmc: PMC5039430pubmed: 27420100google scholar: lookup
  54. Moreira R, García A, Ahumada C, Badía C, Suárez P, Yangari B, Aguayo C, Herrera J, Espejo G, Pinto E. Report of 2018 equine influenza outbreak in Chile. Austral J. Vet. Sci. 2019;51:27–31.
    doi: 10.4067/S0719-81322019000100105google scholar: lookup
  55. Yongfeng Y, Xiaobo S, Nan X, Jingwen Z, Wenqiang L. Detection of the epidemic of the H3N8 subtype of the equine influenza virus in large-scale donkey farms. Int. J. Vet. Sci. Med. 2020;8:26–30.
    doi: 10.1080/23144599.2020.1739844pmc: PMC7170389pubmed: 32341914google scholar: lookup
  56. Bryans J.T, Crowe M.E, Doll E.R, Mccollum W.H. Isolation of a filterable agent causing arteritis of horses and abortion by mares; its differentiation from the equine abortion (influenza) virus. Cornell Vet. 1957;47:3–41.
    pubmed: 13397177
  57. Balasuriya U.B, Go Y.Y, MacLachlan N.J. Equine arteritis virus. Vet. Microbiol. 2013;167:93–122.
    doi: 10.1016/j.vetmic.2013.06.015pmc: PMC7126873pubmed: 23891306google scholar: lookup
  58. Stadejek T, Mittelholzer C, Oleksiewicz M.B, Paweska J, Belák S. Highly diverse type of equine arteritis virus (EAV) from the semen of a South African donkey: Short communication. Acta Vet. Hung. 2006;54:263–270.
    doi: 10.1556/avet.54.2006.2.12pubmed: 16841763google scholar: lookup
  59. Yildirim Y, Kirmizigul H.A, Tan T, Gokce E, Irmak K. Seroprevalence of Equine Viral Arteritis in Donkeys in Kars District, Turkey. J. Anim. Vet. Adv. 2008;7:1110–1112.
  60. Gür S, Irehan B, Gürçay M, Turan T. Serological investigation of equine viral arteritis in donkeys in eastern and south-eastern Anatolia regions of Turkey. Acta Vet. Brno. 2019;88:385–391.
    doi: 10.2754/avb201988040385google scholar: lookup
  61. Chenchev I, Rusenova N, Sandev N. Sero-Epidemiological Studies of Donkeys’ Blood for Detection of Some Virus Infections on Ungulates. Trakia J. Sci. 2011;9:82–86.
  62. Ministry of Agriculture, Forestry and Water Management of Republic of Serbia, Veterinary Directorate Rulebook on Establishing the Program of Animal Health Protection Measures of the Republic of Serbia for 2022. [(accessed on 14 May 2023)]; Available online: https://www.vet.minpolj.gov.rs/dokumenta/program-mera-za-2022-godinu.
  63. World Organization for Animal Health (WOAH) Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, Chapter: 3.6.6 Equine Infectious Anaemia. [(accessed on 6 March 2023)]. Available online: https://www.woah.org/fileadmin/Home/eng/Health_standards/tahm/3.06.06_EIA.pdf.
  64. World Organization for Animal Health (WOAH) Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, Chapter: 3.6.7, Equine Influenza (Infection with Equine Influenza Virus) [(accessed on 14 February 2023)]. Available online: https://www.woah.org/fileadmin/Home/eng/Health_standards/tahm/3.06.07_EQ_INF.pdf.
  65. Singh R.K, Dhama K, Karthik K, Khandia R, Munjal A, Khurana S.K, Chakraborty S, Malik Y.S, Virmani N, Singh R. A Comprehensive Review on Equine Influenza Virus: Etiology, Epidemiology, Pathobiology, Advances in Developing Diagnostics, Vaccines, and Control Strategies. Front. Microbiol. 2018;9:1941.
    doi: 10.3389/fmicb.2018.01941pmc: PMC6135912pubmed: 30237788google scholar: lookup
  66. World Organization for Animal Health (WOAH) Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, Chapter: 3.6.10 Equine Viral Arteritis (Infection with Equine Arteritis Virus) [(accessed on 14 February 2023)]. Available online: https://www.woah.org/fileadmin/Home/eng/Health_standards/tahm/3.06.10_EVA.pdf.
  67. Cook S.J, Cook R.F, Montelaro R.C, Issel C.J. Differential responses of Equus caballus and Equus asinus to infection with two pathogenic strains of equine infectious anemia virus. Vet. Microbiol. 2001;79:93–109.
    doi: 10.1016/S0378-1135(00)00348-5pubmed: 11230932google scholar: lookup
  68. Vidić B, Savić S, Grgić Ž, Bugarski D, Lupulović D, Prica N, Marčić D. Serosurveillance of equine infectious anaemia in a region of vojvodina. Arch. Vet. Med. 2015;7:3–12.
    doi: 10.46784/e-avm.v7i2.127google scholar: lookup
  69. Gaudaire D, Savic S, Lupulovic D, Amelot G, Hans A. Evidence of Equine Infectious Anemia Virus (EIAV) in horses from Serbia. J. Equine Vet. Sci. 2016;39:S41–S42.
    doi: 10.1016/j.jevs.2016.02.091google scholar: lookup
  70. Savić S, Gaudaire D, Lupulović D, Amelot G, Hans A. Evidence of Equine Infectious Anemia Virus (EIAV) in Horses from Serbia. Proceedings of the XVIIth international symposium of the world association of veterinary laboratory diagnosticians (WAVLD) Saskatoon, SK, Canada. 15–18 June 2015.
  71. Thompson G.M, Jess S, Murchie A.K. A review of African horse sickness and its implications for Ireland. Ir. Vet. J. 2012;65:9.
    doi: 10.1186/2046-0481-65-9pmc: PMC3390273pubmed: 22553991google scholar: lookup
  72. Robin M, Archer D, Garros C, Gardès L, Baylis M. The threat of midge-borne equine disease: Investigation of Culicoides species on UK equine premises. Vet. Rec. 2014;174:301.
    doi: 10.1136/vr.102151pubmed: 24508765google scholar: lookup
  73. Trailović D, Krstić V, Đuričić B, Lazić S, Jermolenko G. Equine Viral Respiratory Diseases and Their Significance in the Actual Horse Pathology in Yugoslavia. Proceedings of the Third Symposium in Animal Clinical Pathology and Therapy “Clinica Veterinaria” Budva, Montenegro. 11–15 June 2001.
  74. Lazic S, Vidic B, Lalic M, Pavlovic R, Djurisic S. Finding of Neutralizing Antibodies for the Herpes Virus in the Blood Serum of Race Horses in Vojvodina [Yugoslavia]. Vet. Glas. (Yugoslavia) 1993;47:387–390.
  75. Trailović D, Lazić S. Proceedings of the Equine Herpesvirus Infection How to React. Book of Papers and Abstracts: XII Conference of Veterinarians of the Republic of Serbia Srpsko Veterinarsko Drustvo; Belgrade, Serbia: 2000. pp. 6–12.

Citations

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  1. Zhu Q, Khan MZ, Peng Y, Wang C. A Comparative Review of Donkey Genetic Resources, Production Traits, and Industrial Utilization: Perspectives from China and Globally. Animals (Basel) 2025 Nov 21;15(23).
    doi: 10.3390/ani15233372pubmed: 41375431google scholar: lookup
  2. Ji Y, Zhao X, Liu W. Detection of equine herpesvirus antibodies in large-scale donkey farms in Liaocheng area. Vet Med Sci 2024 Sep;10(5):e70016.
    doi: 10.1002/vms3.70016pubmed: 39268675google scholar: lookup
  3. Li L, Li S, Ma H, Akhtar MF, Tan Y, Wang T, Liu W, Khan A, Khan MZ, Wang C. An Overview of Infectious and Non-Infectious Causes of Pregnancy Losses in Equine. Animals (Basel) 2024 Jul 2;14(13).
    doi: 10.3390/ani14131961pubmed: 38998073google scholar: lookup
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