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Home / Equine Research Bank / Vet Microbiol / Serological survey of domestic animals for tick-borne enceph...
Veterinary microbiology2008; 135(3-4); 267-271; doi: 10.1016/j.vetmic.2008.09.082

Serological survey of domestic animals for tick-borne encephalitis and Bhanja viruses in northeastern Hungary.

Abstract: Blood sera collected from 400 domestic animals (260 cattle, 100 Merino sheep, and 40 Hutzul horses) in northeastern Hungary in 2005 were examined for antibodies against two tick-borne viruses, tick-borne encephalitis flavivirus (TBEV) and Bhanja bunyavirus (BHAV). Using ELISA as screening test and plaque-reduction neutralization as confirmatory test, seropositivity to TBEV was found to be 26.5% in cattle, 7.0% in sheep, and 0.0% in horses. Among cattle, the animals up to 3 years old had significantly lower seroprevalence rate than those in older age groups. Natural foci of tick-borne encephalitis are obviously present in northeastern Hungary. On the other hand, no antibodies neutralizing BHAV were detected in the domestic animals.
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Publication Date: 2008-10-09 PubMed ID: 19036537DOI: 10.1016/j.vetmic.2008.09.082Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research paper explores the prevalence of two tick-borne viruses, tick-borne encephalitis flavivirus (TBEV) and Bhanja bunyavirus (BHAV), in domestic animals (cattle, sheep, and horses) in northeastern Hungary by examining blood serum samples.

Study Overview

  • The study involved serological tests conducted on 400 domestic animals including 260 cattle, 100 Merino sheep, and 40 Hutzul horses in northeastern Hungary in the year 2005.
  • The purpose of the study was to test for the presence of antibodies against two tick-borne viruses, namely tick-borne encephalitis flavivirus (TBEV) and Bhanja bunyavirus (BHAV).

Methodology

  • Enzyme-Linked Immunosorbent Assay (ELISA) was used as the initial screening test followed by a plaque-reduction neutralization test as a confirmatory measure.
  • The ELISA test is a commonly used method for detecting antibodies in blood serum, whilst the plaque-reduction neutralization test provides more specific results for the presence of the neutralizing antibodies.

Key Findings

  • The seropositivity for TBEV was found to be 26.5% in cattle, 7.0% in sheep, and 0.0% in horses, indicating that the virus is more prevalent in cows compared to sheep and non-existent in the tested horses.
  • The seroprevalence rate of TBEV was significantly lower in animals up to 3 years old compared to older ones among cattle. This suggests that the chance of contracting the virus increases with age in cattle.
  • The study thus concludes that northeastern Hungary is a natural focus for tick-borne encephalitis.
  • Conversely, no antibodies neutralizing BHAV were detected in any of the domestic animals, hinting that the BHAV virus, unlike TBEV, was not prevalent in the domestic animals in the tested area during the study period.

Cite This Article

APA
Sikutová S, Hornok S, Hubálek Z, Dolezálková I, Juricová Z, Rudolf I. (2008). Serological survey of domestic animals for tick-borne encephalitis and Bhanja viruses in northeastern Hungary. Vet Microbiol, 135(3-4), 267-271. https://doi.org/10.1016/j.vetmic.2008.09.082

Publication

Veterinary microbiology
ISSN: 0378-1135
NlmUniqueID: 7705469
Country: Netherlands
Language: English
Volume: 135
Issue: 3-4
Pages: 267-271

Researcher Affiliations

Sikutová, Silvie
  • Institute of Vertebrate Biology, v.v.i., Academy of Sciences of the Czech Republic, Medical Zoology Laboratory, Kvetná 8, Brno 60365, Czech Republic. sikutova@ivb.cz
Hornok, Sándor
    Hubálek, Zdenek
      Dolezálková, Iva
        Juricová, Zina
          Rudolf, Ivo

            MeSH Terms

            • Animals
            • Antibodies, Viral / blood
            • Bunyaviridae Infections / epidemiology
            • Bunyaviridae Infections / immunology
            • Bunyaviridae Infections / veterinary
            • Cattle
            • Cattle Diseases / epidemiology
            • Cattle Diseases / virology
            • Encephalitis Viruses, Tick-Borne / isolation & purification
            • Encephalitis, Tick-Borne / epidemiology
            • Encephalitis, Tick-Borne / immunology
            • Encephalitis, Tick-Borne / veterinary
            • Enzyme-Linked Immunosorbent Assay
            • Geography
            • Horse Diseases / epidemiology
            • Horse Diseases / virology
            • Horses
            • Hungary / epidemiology
            • Neutralization Tests
            • Orthobunyavirus / isolation & purification
            • Serotyping
            • Sheep Diseases / epidemiology
            • Sheep Diseases / virology
            • Viral Plaque Assay

            Citations

            This article has been cited 15 times.
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