Serum homocysteine and oxidative stress evaluation during exercise in horse.
Abstract: The aim of the present work was to evaluate the influence of exercise on serum homocysteine and oxidative stress in Thoroughbreds during official races. For our study, ten Thoroughbred mares, 5 years old and clinically healthy, were used. The horses were trained to take part in an official 2100 meter race. Reactive oxygen species (dROMs), antioxidant barrier (Oxy-adsorbent), thiol antioxidant barrier (SHp) and homocysteine (sHcy) were investigated. Blood samples, collected from each horse at rest, immediately after the race, and 30 and 180 min after the end of the race, were collected by jugular venipuncture using vacutainer tubes with no additive for the assessment of dROMs, Oxy-adsorbent and SHp by means of a UV spectrophotometer, while serum total Hcy values were determined by high performance liquid chromatography with fluorimetric detection and isocratic elution. Statistical analysis, one way repeated measures analysis of variance (ANOVA), followed by Bonferroni's test, showed statistical differences (P<0.05 was considered statistically significant) for all parameters studied. Moreover, a coefficient of linear correlation (r) was computed for values of dROMs and sHcy for all sampling times but a significant linear regression (r = 0.94) was found only after the race. The variations of sHcy, dROMs, Oxy-adsorbent and SHp during the experimental period considered suggest the important role of oxidative status in the athletic horse. The systematic analysis of oxidative stress and its influence on homocysteine levels contribute to the clinical evaluation and assessment of the athletic performance of the horse.
Publication Date: 2009-08-04 PubMed ID: 19645345
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- Journal Article
Summary
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This research examines how exercise impacts levels of homocysteine (an amino acid associated with heart disease) and oxidative stress in racehorses. The study determined that exercise results in changes in homocysteine levels and oxidative stress, which plays a significant role in the horses’ athletic performance.
Research Procedure
- The researchers used 10 clinically healthy, 5-year-old Thoroughbred mares for this study.
- The horses were trained to compete in an official 2100-meter race.
- Blood samples were collected from each horse at four instances: at rest, immediately after the race, 30 minutes after the race, and 180 minutes after the race.
- The blood samples were collected through jugular venipuncture using vacutainer tubes.
Investigated Parameter
- The researchers investigated the levels of reactive oxygen species (dROMs), antioxidant barrier (Oxy-adsorbent), thiol antioxidant barrier (SHp), and homocysteine (sHcy).
- dROMs, Oxy-adsorbent, and SHp were evaluated using a UV spectrophotometer.
- Homocysteine values were determined through high-performance liquid chromatography with fluorimetric detection and isocratic elution.
Statistical Analysis and Outcome
- Statistical analysis was conducted using one-way repeated measures analysis of variance (ANOVA) followed by Bonferroni’s test.
- A statistically significant difference was found for all parameters studied (P<0.05).
- A coefficient of linear correlation was computed between the values of dROMs and sHcy at all sampled times, with a significant linear regression found only after the race.
- The variations in sHcy, dROMs, Oxy-adsorbent and SHp suggest the important role of oxidative status in the athletic performance of the horse.
Study Significance
- This research underscores the significant role oxidative stress plays in the athletic performance of horses.
- The study also shows that measuring oxidative stress and its impact on homocysteine levels can contribute to the clinical evaluation and assessment of a racehorse’s athletic performance.
Cite This Article
APA
Fazio F, Casella S, Giannetto C, Caola G, Piccione G.
(2009).
Serum homocysteine and oxidative stress evaluation during exercise in horse.
Pol J Vet Sci, 12(2), 169-174.
Publication
Researcher Affiliations
- Department of Experimental Sciences and Applied Biotechnology, Polo Universitario Annunziata, Faculty of Veterinary Medicine, University of Messina, 98168, Messina, Italy.
MeSH Terms
- Animals
- Female
- Homocysteine / blood
- Horses / blood
- Horses / physiology
- Oxidative Stress / physiology
- Physical Conditioning, Animal / physiology
- Reactive Oxygen Species / blood
Citations
This article has been cited 5 times.- Gołyński M, Metyk M, Ciszewska J, Szczepanik MP, Fitch G, Bęczkowski PM. Homocysteine-Potential Novel Diagnostic Indicator of Health and Disease in Horses. Animals (Basel) 2023 Apr 11;13(8).
- Arfuso F, Rizzo M, Giannetto C, Giudice E, Cirincione R, Cassata G, Cicero L, Piccione G. Oxidant and Antioxidant Parameters' Assessment Together with Homocysteine and Muscle Enzymes in Racehorses: Evaluation of Positive Effects of Exercise. Antioxidants (Basel) 2022 Jun 15;11(6).
- Tafuri S, Cocchia N, Carotenuto D, Vassetti A, Staropoli A, Mastellone V, Peretti V, Ciotola F, Albarella S, Del Prete C, Palumbo V, Esposito L, Vinale F, Ciani F. Chemical Analysis of Lepidium meyenii (Maca) and Its Effects on Redox Status and on Reproductive Biology in Stallions (†). Molecules 2019 May 23;24(10).
- Taniguchi S, Hanafusa M, Tsubone H, Takimoto H, Yamanaka D, Kuwahara M, Ito K. Age-dependency of the serum oxidative level in the senescence-accelerated mouse prone 8. J Vet Med Sci 2016 Sep 1;78(8):1369-71.
- Tsubone H, Hanafusa M, Endo M, Manabe N, Hiraga A, Ohmura H, Aida H. Effect of Treadmill Exercise and Hydrogen-rich Water Intake on Serum Oxidative and Anti-oxidative Metabolites in Serum of Thoroughbred Horses. J Equine Sci 2013;24(1):1-8.
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