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Shipping duration and temperature influence the characteristics of cryopreserved horse semen stored in different shipping devices for up to 14 days.
Abstract: This study aimed to investigate the effects of storing horse semen either in a dry shipper (≤ -150 °C) or on dry ice (≤ -78 °C) for up to 14 days. A total of 264 frozen semen straws from male horses (n = 8) stored in liquid nitrogen were transferred on day 0 (d0) to a dry shipper or a dry ice styrofoam box. On d1, d3, d7, d10, and d14, straws from the dry shipper and dry ice were returned to the liquid nitrogen container. Semen was evaluated by CASA for total (TMot), progressive motility (PMot) and sperm velocity parameters, by fluorescence microscopy for percentage of membrane-intact sperm (SYBR14/PI), high mitochondrial membrane potential (HMMP; JC1) and DNA fragmentation. Temperature inside the containers was monitored continuously. Until d7, no changes were observed in TMot, PMot, and membrane-intact spermatozoa. Thereafter, all three parameters decreased in semen stored on dry ice but not in a dry shipper (time p < 0.001, time x shipping device p < 0.001). The HMMP decreased continuously over time in both containers with a more pronounced decrease on dry ice compared to the dry shipper (shipping device p < 0.01, time p < 0.001, time x device p < 0.001). The DNA fragmentation increased on d10-14 on dry ice and d14 in the dry shipper (time p < 0.001, time x device p < 0.01). In conclusion, frozen horse semen can be safely stored for up to 7 days on dry ice. Sperm DNA integrity and HMMP, however, were adversely affected after 14 days in both shipping devices.
Copyright © 2023 Elsevier B.V. All rights reserved.
Publication Date: 2023-07-23 PubMed ID: 37499284DOI: 10.1016/j.anireprosci.2023.107307Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
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The study explored the effects of storing frozen horse semen in two different conditions: a dry shipper (≤ -150 °C) and on dry ice (≤ -78 °C) for up to 14 days. The results showed that while semen can be safely stored on dry ice for up to 7 days, both storage methods negatively impacted sperm DNA integrity and mitochondrial function after 14 days.
Objective
- Examine the impact of storing horse semen in a dry shipper or on dry ice for a duration of up to 14 days.
Methodology
- 264 frozen semen straws from 8 male horses, initially stored in liquid nitrogen, were transferred to either a dry shipper or a dry ice styrofoam box on day 0 (d0).
- On specific days (d1, d3, d7, d10, and d14), straws from both storage methods were returned to the liquid nitrogen container.
- Assessment of semen included:
- Evaluation of total motility (TMot), progressive motility (PMot), and sperm velocity using CASA.
- Fluorescence microscopy to determine percentage of membrane-intact sperm, high mitochondrial membrane potential (HMMP), and DNA fragmentation.
- Continuous monitoring of the temperature inside the storage containers.
Results
- Up to day 7 (d7), no significant changes were observed in TMot, PMot, and membrane-intact sperm.
- After d7, these parameters decreased for semen stored on dry ice, but not in the dry shipper.
- HMMP consistently decreased over time in both storage methods, with a more pronounced decrease observed in the dry ice storage.
- DNA fragmentation increased on days 10-14 for semen stored on dry ice and on day 14 for semen in the dry shipper.
Conclusion
- Frozen horse semen can be safely stored on dry ice for up to 7 days without significant degradation.
- However, after 14 days, both storage methods (dry shipper and dry ice) negatively affected sperm DNA integrity and mitochondrial function.
Cite This Article
APA
El-Shalofy A, Gautier C, Khan Y, Aurich J, Aurich C.
(2023).
Shipping duration and temperature influence the characteristics of cryopreserved horse semen stored in different shipping devices for up to 14 days.
Anim Reprod Sci, 256, 107307.
https://doi.org/10.1016/j.anireprosci.2023.107307 Publication
Researcher Affiliations
- Artificial Insemination and Embryo Transfer, Department for Small Animals and Horses, University of Veterinary Medicine, Vienna, Austria; Theriogenology Department, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt. Electronic address: Amr.El-Shalofy@vetmeduni.ac.at.
- Artificial Insemination and Embryo Transfer, Department for Small Animals and Horses, University of Veterinary Medicine, Vienna, Austria.
- Artificial Insemination and Embryo Transfer, Department for Small Animals and Horses, University of Veterinary Medicine, Vienna, Austria.
- Obstetrics, Gynecology and Andrology, Department for Small Animals and Horses, University of Veterinary Medicine, Vienna, Austria.
- Artificial Insemination and Embryo Transfer, Department for Small Animals and Horses, University of Veterinary Medicine, Vienna, Austria.
Conflict of Interest Statement
Declaration of Competing Interest The authors state that there is no conflict of interest.
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