Simultaneous quantification of free curcuminoids and their metabolites in equine plasma by LC-ESI-MS/MS.
Abstract: The human health benefits attributed to turmeric/curcumin spice has resulted in its wide utilization as a dietary supplement for companion pets and other animals including horses. While the quantification of free curcuminoids (curcumin, demethoxycurcumin, bisdemethoxycurcumin) and their phase-2 metabolites (curcumin-O-sulfate, curcumin-O-glucuronide) have been extensively investigated in human and rodent biological samples (primarily plasma and serum), there is lack of similar data for horses. Herein, we report a validated LC-ESI-MS/MS method for the simultaneous quantification of the aforementioned free curcuminoids and their metabolites in equine plasma. The linearity of the aforementioned curcuminoids and curcumin-O-sulfate was in the range of 0.5-1000 ng/mL and 1-1000 ng/mL for curcumin-O-glucuronide with 85-115% accuracy and <15% precision in equine plasma. The method was validated based on US FDA criteria and applied to characterize the pharmacokinetics of curcumin-O-sulfate in equine plasma.
Copyright © 2018 Elsevier B.V. All rights reserved.
Publication Date: 2018-03-07 PubMed ID: 29529492DOI: 10.1016/j.jpba.2018.03.014Google Scholar: Lookup
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- Journal Article
- Validation Study
Summary
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This research paper presents a method for measuring the levels of curcuminoids and their metabolic products in horse blood. It examines the use of turmeric/curcumin as a dietary supplement for animals like horses using Liquid Chromatography with Mass Spectrometry/Mass Spectrometry (LC-ESI-MS/MS), a common tool used in drug discovery.
Background
- Turmeric, and its primary active compound, curcumin, have been attributed with significant health benefits in humans, leading to its widespread use in dietary supplements. These supplements aren’t limited to human consumption, and are also used in companion pets like cats, dogs, and other animals, including horses.
- While the different types of curcuminoids (curcumin, demethoxycurcumin, and bisdemethoxycurcumin) and their metabolic byproducts (curcumin-O-sulfate and curcumin-O-glucuronide) have been widely studied in human and rodent samples, there’s a noticeable lack of similar data for horses.
Methodology
- The study uses LC-ESI-MS/MS, a common bioanalytical method, for the simultaneous measurement of the target curcuminoids and their metabolites in equine plasma.
- The established method was validated against the U.S. Food and Drug Administration (FDA) criteria.
Results
- The range in which the curcuminoids and curcumin-O-sulfate could be effectively measured was between 0.5-1000 ng/mL, and 1-1000 ng/mL for curcumin-O-glucuronide. This was with a stated accuracy of 85-115% and a precision of under 15% in the equine plasma.
- The validated method was then applied to determine the behaviour of curcumin-O-sulfate in horse blood over time, or its pharmacokinetics.
Through this research, a validated process now exists to quantify and understand the impact of dietary turmeric/curcumin supplement consumption on equine health. This can be used to further study dosage, efficacy and any potential side effects.
Cite This Article
APA
Liu Y, Siard M, Adams A, Keowen ML, Miller TK, Garza F, Andrews FM, Seeram NP.
(2018).
Simultaneous quantification of free curcuminoids and their metabolites in equine plasma by LC-ESI-MS/MS.
J Pharm Biomed Anal, 154, 31-39.
https://doi.org/10.1016/j.jpba.2018.03.014 Publication
Researcher Affiliations
- Bioactive Botanical Research Laboratory, Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy, University of Rhode Island, 7 Greenhouse Road, Kingston, RI, 02881, USA.
- Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, 1400 Nicholasville Road, Lexington, KY, 40546, USA.
- Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, 1400 Nicholasville Road, Lexington, KY, 40546, USA.
- Equine Health Studies Program, Department of Veterinary Clinical Sciences, Louisiana State University School of Veterinary Medicine, Skip Bertman Drive, 1843 Veterinary Teaching Hospital, Baton Rouge, LA, 70803, USA.
- Equine Health Studies Program, Department of Veterinary Clinical Sciences, Louisiana State University School of Veterinary Medicine, Skip Bertman Drive, 1843 Veterinary Teaching Hospital, Baton Rouge, LA, 70803, USA.
- Equine Health Studies Program, Department of Veterinary Clinical Sciences, Louisiana State University School of Veterinary Medicine, Skip Bertman Drive, 1843 Veterinary Teaching Hospital, Baton Rouge, LA, 70803, USA.
- Equine Health Studies Program, Department of Veterinary Clinical Sciences, Louisiana State University School of Veterinary Medicine, Skip Bertman Drive, 1843 Veterinary Teaching Hospital, Baton Rouge, LA, 70803, USA.
- Bioactive Botanical Research Laboratory, Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy, University of Rhode Island, 7 Greenhouse Road, Kingston, RI, 02881, USA. Electronic address: nseeram@uri.edu.
MeSH Terms
- Animals
- Chromatography, High Pressure Liquid / instrumentation
- Chromatography, High Pressure Liquid / methods
- Chromatography, High Pressure Liquid / veterinary
- Curcuma / chemistry
- Curcumin / analogs & derivatives
- Curcumin / analysis
- Curcumin / metabolism
- Horses
- Spectrometry, Mass, Electrospray Ionization / instrumentation
- Spectrometry, Mass, Electrospray Ionization / methods
- Spectrometry, Mass, Electrospray Ionization / veterinary
- Tandem Mass Spectrometry / instrumentation
- Tandem Mass Spectrometry / methods
- Tandem Mass Spectrometry / veterinary
Citations
This article has been cited 9 times.- Zagórska J, Kukula-Koch W, Czop M, Iłowiecka K, Koch W. Impact of Thermal Processing on the Composition of Curcuma longa Rhizome. Foods 2023 Aug 17;12(16).
- Taguchi T, Morales Yniguez FJ, Takawira C, Andrews FM, Lopez MJ. Agmatine Administration Effects on Equine Gastric Ulceration and Lameness. J Clin Med 2022 Dec 8;11(24).
- Amante C, Andretto V, Rosso A, Augusti G, Marzocco S, Lollo G, Del Gaudio P. Alginate-pectin microparticles loaded with nanoemulsions as nanocomposites for wound healing. Drug Deliv Transl Res 2023 May;13(5):1343-1357.
- Kotra VSR, Satyabanta L, Goswami TK. A critical review of analytical methods for determination of curcuminoids in turmeric. J Food Sci Technol 2019 Dec;56(12):5153-5166.
- Kotha RR, Luthria DL. Curcumin: Biological, Pharmaceutical, Nutraceutical, and Analytical Aspects. Molecules 2019 Aug 13;24(16).
- Zhang B, Yang J, Qin Z, Li S, Xu J, Yao Z, Zhang X, Gonzalez FJ, Yao X. Mechanism of the efflux transport of demethoxycurcumin-O-glucuronides in HeLa cells stably transfected with UDP-glucuronosyltransferase 1A1. PLoS One 2019;14(5):e0217695.
- Wei W, Yu Y, Wang X, Yang L, Zhang H, Ji H, Li Z, Hou J, Wu W, Guo D. Simultaneous Determination of Bufalin and Its Nine Metabolites in Rat Plasma for Characterization of Metabolic Profiles and Pharmacokinetic Study by LC⁻MS/MS. Molecules 2019 Apr 28;24(9).
- Johnson SL, Park HY, DaSilva NA, Vattem DA, Ma H, Seeram NP. Levodopa-Reduced Mucuna pruriens Seed Extract Shows Neuroprotective Effects against Parkinson's Disease in Murine Microglia and Human Neuroblastoma Cells, Caenorhabditis elegans, and Drosophila melanogaster. Nutrients 2018 Aug 22;10(9).
- Alallam B, Abdkadir E, Hayati A, Keong YY, Lim V. Alginate coated mesoporous silica nanoparticles as oral delivery carrier of curcumin and quercetin to colon cancer: preparation, optimization, characterization, and anticancer activity. Drug Deliv Transl Res 2025 Nov;15(11):3793-3834.
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