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PloS one2017; 12(11); e0186247; doi: 10.1371/journal.pone.0186247

Skeletal muscle mitochondrial bioenergetics and associations with myostatin genotypes in the Thoroughbred horse.

Abstract: Variation in the myostatin (MSTN) gene has been reported to be associated with race distance, body composition and skeletal muscle fibre composition in the horse. The aim of the present study was to test the hypothesis that MSTN variation influences mitochondrial phenotypes in equine skeletal muscle. Mitochondrial abundance and skeletal muscle fibre types were measured in whole muscle biopsies from the gluteus medius of n = 82 untrained (21 ± 3 months) Thoroughbred horses. Skeletal muscle fibre type proportions were significantly (p < 0.01) different among the three MSTN genotypes and mitochondrial content was significantly (p T (C) and the SINE insertion 227 bp polymorphism (I). Evaluation of mitochondrial complex activities indicated higher combined mitochondrial complex I+III and II+III activities in the presence of the C-allele / I allele (p ≤ 0.05). The restoration of complex I+III and complex II+III activities following addition of exogenous coenzyme Q1 (ubiquinone1) (CoQ1) in vitro in the TT/NN (homozygous T allele/homozygous no insertion) cohort indicated decreased coenzyme Q in these animals. In addition, decreased gene expression in two coenzyme Q (CoQ) biosynthesis pathway genes (COQ4, p ≤ 0.05; ADCK3, p ≤ 0.01) in the TT/NN horses was observed. This study has identified several mitochondrial phenotypes associated with MSTN genotype in untrained Thoroughbred horses and in addition, our findings suggest that nutritional supplementation with CoQ may aid to restore coenzyme Q activity in TT/NN horses.
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Publication Date: 2017-11-30 PubMed ID: 29190290PubMed Central: PMC5708611DOI: 10.1371/journal.pone.0186247Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research explored the impact of myostatin (MSTN) genetic variations on the mitochondrial characteristics in horse muscle tissue. The study demonstrated a significant link between MSTN variations and mitochondrial activity, and proposed this may be potential area of focus for nutritional supplementation.

Objective of the Research

The study aimed to explore the possible influence of myostatin (MSTN) variation on mitochondrial features in the skeletal muscles of untrained Thoroughbred horses. The hypothesis was based on the previous reports suggesting an association between MSTN gene variation and several physical attributes in horses, such as their capability for racing over different distances, body composition, and skeletal muscle fiber composition.

Research Methodology

  • The research was conducted on whole muscle biopsies from the gluteus medius of 82 untrained Thoroughbred horses, aged 21 ± 3 months.
  • Researchers analyzed the mitochondrial abundance and skeletal muscle fibre types in these samples.
  • They specifically looked for the presence of MSTN genotypes, including the C-allele of SNP g.66493737C>T and the SINE insertion 227 bp polymorphism.

Findings of the Research

  • The skeletal muscle fibre type proportions were found to be significantly different among the horses, depending on their MSTN genotype.
  • Notably, mitochondrial content was found to be significantly lower in horses carrying the combined presence of the C-allele and the SINE insertion polymorphism.
  • Upon evaluation of mitochondrial complex activities, they exhibited higher combined mitochondrial complex I+III and II+III activities in horses with the C-allele and the SINE insertion polymorphism.
  • The team also observed decreased gene regulations within two coenzyme Q (CoQ) biosynthesis pathway genes (COQ4 and ADCK3) in the TT/NN horses, indicating decreased coenzyme Q levels in them.

Conclusions and Implications

  • The results of this study reveal several mitochondrial phenotypes associated with differences in MSTN genotype in untrained Thoroughbred horses.
  • The findings suggest that dietary supplementation with coenzyme Q (CoQ) may assist in the restoration of coenzyme Q activity in horses carrying certain MSTN genotypes, particularly TT/NN genotype horses. This could eventually contribute to the improvement of the horses’ physical performance.

Cite This Article

APA
Rooney MF, Porter RK, Katz LM, Hill EW. (2017). Skeletal muscle mitochondrial bioenergetics and associations with myostatin genotypes in the Thoroughbred horse. PLoS One, 12(11), e0186247. https://doi.org/10.1371/journal.pone.0186247

Publication

PloS one
ISSN: 1932-6203
NlmUniqueID: 101285081
Country: United States
Language: English
Volume: 12
Issue: 11
Pages: e0186247
PII: e0186247

Researcher Affiliations

Rooney, Mary F
  • UCD School of Agriculture and Food Science, University College Dublin, Belfield, Dublin 4, Ireland.
  • School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute (TBSI), Trinity College Dublin, Dublin 2, Ireland.
Porter, Richard K
  • School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute (TBSI), Trinity College Dublin, Dublin 2, Ireland.
Katz, Lisa M
  • UCD School of Veterinary Medicine, University College Dublin, Belfield, Dublin 4, Ireland.
Hill, Emmeline W
  • UCD School of Agriculture and Food Science, University College Dublin, Belfield, Dublin 4, Ireland.

MeSH Terms

  • Age Factors
  • Animals
  • Energy Metabolism
  • Female
  • Genotype
  • Horses / genetics
  • Horses / metabolism
  • Male
  • Mitochondria, Muscle / metabolism
  • Muscle, Skeletal / metabolism
  • Myostatin / genetics
  • Phenotype
  • Physical Conditioning, Animal
  • Polymorphism, Single Nucleotide
  • Short Interspersed Nucleotide Elements

Conflict of Interest Statement

A patent application (EP17190252.1) entitled “A Genotype specific Nutritional Supplement for Horses” has been submitted to the European Patent Office, based on these research findings. All authors of this manuscript are named on this patent application. This does not alter our adherence to PLOS ONE policies on sharing data and materials.

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Citations

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