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The Journal of general virology2018; 100(1); 7-25; doi: 10.1099/jgv.0.001171

Spread of the emerging equine-like G3P[8] DS-1-like genetic backbone rotavirus strain in Brazil and identification of potential genetic variants.

Abstract: In 2013, the equine-like G3P[8] DS-1-like rotavirus (RVA) strain emerged worldwide. In 2016, this strain was reported in northern Brazil. The aims of the study were to conduct a retrospective genetic investigation to identify the possible entry of these atypical strains in Brazil and to describe their distribution across a representative area of the country. From 2013 to 2017, a total of 4226 faecal samples were screened for RVA by ELISA, PAGE, RT-PCR and sequencing. G3P[8] represented 20.9 % (167/800) of all RVA-positive samples, further subdivided as equine-like G3P[8], DS-1-like (11.0 %; 88/800) and Wa-like G3P[8] (9.9 %; 79/800). Six equine-like G3P[8] DS-1-like samples were selected for whole-genome investigation, confirming the backbone I2-R2-C2-M2-A2-N2-T2-E2-H2. During 2013-2014, Wa-like G3P[8] was predominant and no equine-like G3P[8] DS-1-like was detected. Equine-like G3P[8] DS-1-like was first identified in Paraná in March/2015, suggesting that the strain entered Brazil through the Southern region. Equine-like G3P[8] rapidly spread across the area under surveillance and displayed a marked potential to replace Wa-like G3P[8] strains. Brazilian equine-like G3P[8] DS-1-like strains clustered with contemporary equine-like G3P[8] DS-1-like detected worldwide, but exhibited a distinct NSP2 genotype (N2) compared to the previously reported Amazon equine-like G3P[8] DS-1-like strain (N1). Two distinct NSP4 E2 genotype lineages were also identified. Taken together, these data suggest that different variants of equine-like G3P[8] DS-1-like strains might have been introduced into the country at distinct time points, and co-circulated in the period 2015-2017. The global emergence of equine-like G3P[8] DS-1-like strains, predominantly in countries using the Rotarix vaccine, raises the question of whether vaccines may be inducing selective pressures on zoonotic strains.
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Publication Date: 2018-11-20 PubMed ID: 30457517DOI: 10.1099/jgv.0.001171Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study investigates the emergence and spread of a unique strain of rotavirus (G3P[8] DS-1-like) in Brazil, with an aim to identify its entry and distribution patterns and whether there are different variants present in the country.

Study Overview

  • The research focused on a particular strain of rotavirus, known as the equine-like G3P[8] DS-1-like, which emerged in 2013 and was detected in Brazil in 2016.
  • The main objective of the study was to identify how and when this rotavirus strain entered Brazil, and to understand how it spread across the representative area of the country. Additionally, the study aimed to identify potential genetic variants of this virus in Brazil.

Methods and Observations

  • The investigators conducted a retrospective genetic investigation, examining a total of 4226 faecal samples collected between 2013 and 2017, using several techniques including ELISA, PAGE, RT-PCR and sequencing.
  • The G3P[8] strain represented 20.9% (167 out of 800) of all rotavirus-positive cases. The strain further classified into equine-like G3P[8], DS-1-like (11%), and Wa-like G3P[8] (9.9%).
  • Through the whole-genome investigation, the presence of backbone I2-R2-C2-M2-A2-N2-T2-E2-H2 in the G3P[8] DS-1-like samples was confirmed.
  • The first identification of G3P[8] DS-1-like was in Paraná in March 2015. This suggests that the strain might have entered Brazil through the Southern region and spread swiftly across the observed region. Interestingly, it exhibited a significant potential to replace the existing Wa-like G3P[8] strains.

Key Outcomes

  • The Brazilian variants of the strain showed close association with other strains detected worldwide. However, these local strains exhibited a distinct NSP2 genotype (N2), different from the previously reported Amazon strain (N1). This illustrates potential variants of the equine-like G3P[8] DS-1-like strains in the country.
  • Two separate NSP4 E2 genotype lineages of the virus were also detected.
  • Putting these findings together, it is suggested that different variants of the strain may have been introduced into Brazil at different times and have co-circulated during the period 2015-2017.
  • The study further highlights a global concern: the rise of strains like G3P[8] DS-1-like in countries using the Rotarix vaccine, raising questions about whether vaccines may be influencing selective pressures on these zoonotic, or animal-origin, strains.

Cite This Article

APA
Luchs A, da Costa AC, Cilli A, Komninakis SCV, Carmona RCC, Boen L, Morillo SG, Sabino EC, Timenetsky MDCST. (2018). Spread of the emerging equine-like G3P[8] DS-1-like genetic backbone rotavirus strain in Brazil and identification of potential genetic variants. J Gen Virol, 100(1), 7-25. https://doi.org/10.1099/jgv.0.001171

Publication

The Journal of general virology
ISSN: 1465-2099
NlmUniqueID: 0077340
Country: England
Language: English
Volume: 100
Issue: 1
Pages: 7-25

Researcher Affiliations

Luchs, Adriana
  • 1​Enteric Disease Laboratory, Virology Center, Adolfo Lutz Institute, São Paulo, Brazil.
da Costa, Antonio Charlys
  • 2​LIM/46 - Laboratory of Medical Parasitology, Department of Infectious and Parasitic Diseases, College of Medicine, University of São Paulo, São Paulo, Brazil.
  • 3​Institute of Tropical Medicine, University of São Paulo, São Paulo, Brazil.
Cilli, Audrey
  • 1​Enteric Disease Laboratory, Virology Center, Adolfo Lutz Institute, São Paulo, Brazil.
Komninakis, Shirley Cavalcante Vasconcelos
  • 4​Postgraduate Program in Health Science, Faculty of Medicine of ABC, Santo André, Brazil.
  • 5​Retrovirology Laboratory, Federal University of São Paulo, São Paulo, São Paulo, Brazil.
Carmona, Rita de Cássia Compagnoli
  • 1​Enteric Disease Laboratory, Virology Center, Adolfo Lutz Institute, São Paulo, Brazil.
Boen, Lais
  • 1​Enteric Disease Laboratory, Virology Center, Adolfo Lutz Institute, São Paulo, Brazil.
Morillo, Simone Guadagnucci
  • 1​Enteric Disease Laboratory, Virology Center, Adolfo Lutz Institute, São Paulo, Brazil.
Sabino, Ester Cerdeira
  • 2​LIM/46 - Laboratory of Medical Parasitology, Department of Infectious and Parasitic Diseases, College of Medicine, University of São Paulo, São Paulo, Brazil.
  • 3​Institute of Tropical Medicine, University of São Paulo, São Paulo, Brazil.
Timenetsky, Maria do Carmo Sampaio Tavares
  • 1​Enteric Disease Laboratory, Virology Center, Adolfo Lutz Institute, São Paulo, Brazil.

MeSH Terms

  • Brazil / epidemiology
  • Electrophoresis, Polyacrylamide Gel
  • Enzyme-Linked Immunosorbent Assay
  • Feces / virology
  • Gastroenteritis / epidemiology
  • Gastroenteritis / virology
  • Genotype
  • Humans
  • Molecular Epidemiology
  • Retrospective Studies
  • Reverse Transcriptase Polymerase Chain Reaction
  • Rotavirus / classification
  • Rotavirus / genetics
  • Rotavirus / isolation & purification
  • Rotavirus Infections / epidemiology
  • Rotavirus Infections / virology
  • Sequence Analysis, DNA
  • Topography, Medical

Citations

This article has been cited 36 times.
  1. Degiuseppe JI, Martelli A, Barrios Mathieur C, Stupka JA. Genetic diversity of rotavirus A in Argentina during 2019-2022: detection of G6 strains and insights regarding its dissemination. Arch Virol 2023 Sep 13;168(10):251.
    doi: 10.1007/s00705-023-05874-8pubmed: 37702836google scholar: lookup
  2. Carossino M, Balasuriya UBR, Thieulent CJ, Barrandeguy ME, Vissani MA, Parreño V. Quadruplex Real-Time TaqMan(®) RT-qPCR Assay for Differentiation of Equine Group A and B Rotaviruses and Identification of Group A G3 and G14 Genotypes. Viruses 2023 Jul 26;15(8).
    doi: 10.3390/v15081626pubmed: 37631969google scholar: lookup
  3. Gutierrez MB, de Assis RMS, Andrade JDSR, Fialho AM, Fumian TM. Rotavirus A during the COVID-19 Pandemic in Brazil, 2020-2022: Emergence of G6P[8] Genotype. Viruses 2023 Jul 25;15(8).
    doi: 10.3390/v15081619pubmed: 37631962google scholar: lookup
  4. Sashina TA, Velikzhanina EI, Morozova OV, Epifanova NV, Novikova NA. Detection and full-genotype determination of rare and reassortant rotavirus A strains in Nizhny Novgorod in the European part of Russia. Arch Virol 2023 Jul 31;168(8):215.
    doi: 10.1007/s00705-023-05838-ypubmed: 37524885google scholar: lookup
  5. Moraes MTB, Silva MFD, Pimenta YC, Cantelli CP, Assis RMS, Fialho AM, Bueno MG, Olivares AIO, Svensson L, Leite JPG, Nordgren J. G6P[8] Rotavirus a Possessing a Wa-like VP3 Gene from a Child with Acute Gastroenteritis Living in the Northwest Amazon Region. Pathogens 2023 Jul 20;12(7).
    doi: 10.3390/pathogens12070956pubmed: 37513803google scholar: lookup
  6. Medeiros RS, França Y, Viana E, de Azevedo LS, Guiducci R, de Lima Neto DF, da Costa AC, Luchs A. Genomic Constellation of Human Rotavirus G8 Strains in Brazil over a 13-Year Period: Detection of the Novel Bovine-like G8P[8] Strains with the DS-1-like Backbone. Viruses 2023 Mar 1;15(3).
    doi: 10.3390/v15030664pubmed: 36992373google scholar: lookup
  7. Pathak A, Gulati BR, Maan S, Mor S, Kumar D, Soman R, Punia S, Chaudhary D, Khurana SK. Complete Genome Sequencing Reveals Unusual Equine Rotavirus A of Bat Origin from India. J Virol 2022 Oct 26;96(20):e0140822.
    doi: 10.1128/jvi.01408-22pubmed: 36214578google scholar: lookup
  8. Lestari FB, Vongpunsawad S, Poovorawan Y. Diverse human and bat-like rotavirus G3 strains circulating in suburban Bangkok. PLoS One 2022;17(5):e0268465.
    doi: 10.1371/journal.pone.0268465pubmed: 35609031google scholar: lookup
  9. Katz EM, Esona MD, Gautam R, Bowen MD. Development of a Real-Time Reverse Transcription-PCR Assay To Detect and Quantify Group A Rotavirus Equine-Like G3 Strains. J Clin Microbiol 2021 Oct 19;59(11):e0260220.
    doi: 10.1128/JCM.02602-20pubmed: 34432486google scholar: lookup
  10. Gutierrez MB, de Figueiredo MR, Fialho AM, Cantelli CP, Miagostovich MP, Fumian TM. Nosocomial acute gastroenteritis outbreak caused by an equine-like G3P[8] DS-1-like rotavirus and GII.4 Sydney[P16] norovirus at a pediatric hospital in Rio de Janeiro, Brazil, 2019. Hum Vaccin Immunother 2021 Nov 2;17(11):4654-4660.
    doi: 10.1080/21645515.2021.1963169pubmed: 34402714google scholar: lookup
  11. Amit LN, Mori D, John JL, Chin AZ, Mosiun AK, Jeffree MS, Ahmed K. Emergence of equine-like G3 strains as the dominant rotavirus among children under five with diarrhea in Sabah, Malaysia  during 2018-2019. PLoS One 2021;16(7):e0254784.
    doi: 10.1371/journal.pone.0254784pubmed: 34320003google scholar: lookup
  12. Martinez-Gutierrez M, Hernandez-Mira E, Rendon-Marin S, Ruiz-Saenz J. Wa-1 Equine-Like G3P[8] Rotavirus from a Child with Diarrhea in Colombia. Viruses 2021 Jun 4;13(6).
    doi: 10.3390/v13061075pubmed: 34199978google scholar: lookup
  13. Wahyuni RM, Utsumi T, Dinana Z, Yamani LN, Juniastuti, Wuwuti IS, Fitriana E, Gunawan E, Liang Y, Ramadhan F, Soetjipto, Lusida MI, Shoji I. Prevalence and Distribution of Rotavirus Genotypes Among Children With Acute Gastroenteritis in Areas Other Than Java Island, Indonesia, 2016-2018. Front Microbiol 2021;12:672837.
    doi: 10.3389/fmicb.2021.672837pubmed: 34025628google scholar: lookup
  14. Thomas S, Donato CM, Covea S, Ratu FT, Jenney AWJ, Reyburn R, Sahu Khan A, Rafai E, Grabovac V, Serhan F, Bines JE, Russell FM. Genotype Diversity before and after the Introduction of a Rotavirus Vaccine into the National Immunisation Program in Fiji. Pathogens 2021 Mar 17;10(3).
    doi: 10.3390/pathogens10030358pubmed: 33802966google scholar: lookup
  15. Silva-Sales M, Leal E, Milagres FAP, Brustulin R, Morais VDS, Marcatti R, Araújo ELL, Witkin SS, Deng X, Sabino EC, Delwart E, Luchs A, Costa ACD. Genomic constellation of human Rotavirus A strains identified in Northern Brazil: a 6-year follow-up (2010-2016). Rev Inst Med Trop Sao Paulo 2020;62:e98.
    doi: 10.1590/S1678-9946202062098pubmed: 33331517google scholar: lookup
  16. Zhou N, Zhou L, Wang B. Genetic Characterizations and Molecular Evolution of VP7 Gene in Human Group A Rotavirus G1. Viruses 2020 Jul 30;12(8).
    doi: 10.3390/v12080831pubmed: 32751603google scholar: lookup
  17. Gutierrez MB, Fialho AM, Maranhão AG, Malta FC, Andrade JDSR, Assis RMS, Mouta SDSE, Miagostovich MP, Leite JPG, Machado Fumian T. Rotavirus A in Brazil: Molecular Epidemiology and Surveillance during 2018-2019. Pathogens 2020 Jun 27;9(7).
    doi: 10.3390/pathogens9070515pubmed: 32605014google scholar: lookup
  18. Chansaenroj J, Chuchaona W, Lestari FB, Pasittungkul S, Klinfueng S, Wanlapakorn N, Vongpunsawad S, Chirathaworn C, Poovorawan Y. High prevalence of DS-1-like rotavirus infection in Thai adults between 2016 and 2019. PLoS One 2020;15(6):e0235280.
    doi: 10.1371/journal.pone.0235280pubmed: 32584905google scholar: lookup
  19. Fukuda S, Tacharoenmuang R, Guntapong R, Upachai S, Singchai P, Ide T, Hatazawa R, Sutthiwarakom K, Kongjorn S, Onvimala N, Ruchusatsawast K, Rungnopakun P, Mekmallika J, Kawamura Y, Motomura K, Tatsumi M, Takeda N, Murata T, Yoshikawa T, Uppapong B, Taniguchi K, Komoto S. Full genome characterization of novel DS-1-like G9P[8] rotavirus strains that have emerged in Thailand. PLoS One 2020;15(4):e0231099.
    doi: 10.1371/journal.pone.0231099pubmed: 32320419google scholar: lookup
  20. Damanka SA, Kwofie S, Dennis FE, Lartey BL, Agbemabiese CA, Doan YH, Adiku TK, Katayama K, Enweronu-Laryea CC, Armah GE. Whole genome characterization and evolutionary analysis of OP354-like P[8] Rotavirus A strains isolated from Ghanaian children with diarrhoea. PLoS One 2019;14(6):e0218348.
    doi: 10.1371/journal.pone.0218348pubmed: 31199823google scholar: lookup
  21. Agbemabiese CA, Nakagomi T, Damanka SA, Dennis FE, Lartey BL, Armah GE, Nakagomi O. Sub-genotype phylogeny of the non-G, non-P genes of genotype 2 Rotavirus A strains. PLoS One 2019;14(5):e0217422.
    doi: 10.1371/journal.pone.0217422pubmed: 31150425google scholar: lookup
  22. Athiyyah AF, Utsumi T, Wahyuni RM, Dinana Z, Yamani LN, Soetjipto, Sudarmo SM, Ranuh RG, Darma A, Juniastuti, Raharjo D, Matsui C, Deng L, Abe T, Doan YH, Fujii Y, Shimizu H, Katayama K, Lusida MI, Shoji I. Molecular Epidemiology and Clinical Features of Rotavirus Infection Among Pediatric Patients in East Java, Indonesia During 2015-2018: Dynamic Changes in Rotavirus Genotypes From Equine-Like G3 to Typical Human G1/G3. Front Microbiol 2019;10:940.
    doi: 10.3389/fmicb.2019.00940pubmed: 31130934google scholar: lookup
  23. Burlandy FM, Casarin BC, Gutierrez MB, Cantelli CP, Novo SPC, de Assis RMS, Piccoli BC, Haisi A, da Costa Daneli A, Marchesan RD, Kuhns J, Araújo Junior JP, Teixeira LB, Schwarzbold AV, de Arruda Trindade P, Miagostovich MP, Fumian TM. Rotavirus Outbreak Traced in Clinical, Environmental, and Food Matrices: Whole Genomic Characterization of an Equine-like G3P[8] Genotype. Food Environ Virol 2026 Jan 31;18(1):7.
    doi: 10.1007/s12560-026-09678-2pubmed: 41619117google scholar: lookup
  24. Silva MF, Silva BVD, Ramalho E, Pimenta YC, Silva LLPD, Vieira LDS, Xavier MDPTP, Olivares AIO, Leite JPG, Moraes MTB. Examination of In Vivo Mutations in VP4 (VP8*) of the Rotarix(®) Vaccine from Shedding of Children Living in the Amazon Region. Viruses 2026 Jan 3;18(1).
    doi: 10.3390/v18010070pubmed: 41600834google scholar: lookup
  25. de Azevedo LS, Silva VCM, França Y, Guiducci R, Luchs A. Tracing the Zoonotic Origins of a Rare Human G5P[6] Rotavirus in Brazil. Pathogens 2025 Nov 17;14(11).
    doi: 10.3390/pathogens14111172pubmed: 41305408google scholar: lookup
  26. Silva VCM, França Y, Azevedo LS, Guiducci R, Villela EFM, Luchs A. Unveiling the Genomic Landscape of G2P[6] Rotavirus a Strains in Brazil: Evolutionary and Epidemiological Perspectives. Viruses 2025 Aug 11;17(8).
    doi: 10.3390/v17081103pubmed: 40872817google scholar: lookup
  27. Vizzi E, Rosales RE, Piñeros O, Fernández R, Inaty D, López K, Peña L, De Freitas-Linares A, Navarro D, Neri S, Durán O, Liprandi F. Emergence of Equine-like G3P[8] Rotavirus Strains Infecting Children in Venezuela. Viruses 2025 Mar 13;17(3).
    doi: 10.3390/v17030410pubmed: 40143336google scholar: lookup
  28. Hosseini-Fakhr SS, Jalilvand S, Maleki A, Kachooei A, Behnezhad F, Mir-Hosseinian M, Taghvaei S, Marashi SM, Shoja Z. Genetic characterization of rotavirus A strains circulating in children under 5 years of age with acute gastroenteritis in Tehran, Iran, in 2023-2024: dissemination of the emerging equine-like G3P[8]-I2-E2 DS-1-like strains. J Gen Virol 2025 Mar;106(3).
    doi: 10.1099/jgv.0.002088pubmed: 40100090google scholar: lookup
  29. Oliveira Matos A, Araujo M, Paulino J, Franco FC, Luchs A, Sales-Campos H, Fiaccadori F, Souza M, Silva-Sales M. Mutations in the main antigenic sites of VP7 and VP8* from G3P[8] rotavirus a strains circulating in Brazil may impact immune evasion to rotavirus vaccination. Braz J Microbiol 2025 Mar;56(1):319-330.
    doi: 10.1007/s42770-024-01542-4pubmed: 39505807google scholar: lookup
  30. França Y, Medeiros RS, Viana E, de Azevedo LS, Guiducci R, da Costa AC, Luchs A. Genetic diversity and evolution of G12P[6] DS-1-like and G12P[9] AU-1-like Rotavirus strains in Brazil. Funct Integr Genomics 2024 May 11;24(3):92.
    doi: 10.1007/s10142-024-01360-9pubmed: 38733534google scholar: lookup
  31. Gutierrez MB, Arantes I, Bello G, Berto LH, Dutra LH, Kato RB, Fumian TM. Emergence and dissemination of equine-like G3P[8] rotavirus A in Brazil between 2015 and 2021. Microbiol Spectr 2024 Apr 2;12(4):e0370923.
    doi: 10.1128/spectrum.03709-23pubmed: 38451227google scholar: lookup
  32. Le LKT, Chu MNT, Tate JE, Jiang B, Bowen MD, Esona MD, Gautam R, Jaimes J, Pham TPT, Huong NT, Anh DD, Trang NV, Parashar U. Genetic diversity of G9, G3, G8 and G1 rotavirus group A strains circulating among children with acute gastroenteritis in Vietnam from 2016 to 2021. Infect Genet Evol 2024 Mar;118:105566.
    doi: 10.1016/j.meegid.2024.105566pubmed: 38316245google scholar: lookup
  33. Carossino M, Vissani MA, Barrandeguy ME, Balasuriya UBR, Parreño V. Equine Rotavirus A under the One Health Lens: Potential Impacts on Public Health. Viruses 2024 Jan 16;16(1).
    doi: 10.3390/v16010130pubmed: 38257830google scholar: lookup
  34. Doan YH, Dennis FE, Takemae N, Haga K, Shimizu H, Appiah MG, Lartey BL, Damanka SA, Hayashi T, Suzuki T, Kageyama T, Armah GE, Katayama K. Emergence of Intergenogroup Reassortant G9P[4] Strains Following Rotavirus Vaccine Introduction in Ghana. Viruses 2023 Dec 18;15(12).
    doi: 10.3390/v15122453pubmed: 38140694google scholar: lookup
  35. Mhango C, Banda A, Chinyama E, Mandolo JJ, Kumwenda O, Malamba-Banda C, Barnes KG, Kumwenda B, Jambo KC, Donato CM, Esona MD, Mwangi PN, Steele AD, Iturriza-Gomara M, Cunliffe NA, Ndze VN, Kamng'ona AW, Dennis FE, Nyaga MM, Chaguza C, Jere KC. Comparative whole genome analysis reveals re-emergence of human Wa-like and DS-1-like G3 rotaviruses after Rotarix vaccine introduction in Malawi. Virus Evol 2023;9(1):vead030.
    doi: 10.1093/ve/vead030pubmed: 37305707google scholar: lookup
  36. Reyes Y, St Jean DT, Bowman NM, González F, Mijatovic-Rustempasic S, Becker-Dreps S, Svensson L, Nordgren J, Bucardo F, Vielot NA. Nonsecretor Phenotype Is Associated With Less Risk of Rotavirus-Associated Acute Gastroenteritis in a Vaccinated Nicaraguan Birth Cohort. J Infect Dis 2023 Dec 20;228(12):1739-1747.
    doi: 10.1093/infdis/jiad202pubmed: 37279878google scholar: lookup
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