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In vitro cellular & developmental biology. Animal2014; 51(3); 230-240; doi: 10.1007/s11626-014-9828-0

Static magnetic field enhances synthesis and secretion of membrane-derived microvesicles (MVs) rich in VEGF and BMP-2 in equine adipose-derived stromal cells (EqASCs)-a new approach in veterinary regenerative medicine.

Abstract: The aim of this work study was to evaluate the cytophysiological activity of equine adipose-derived stem cells (ASCs) cultured under conditions of static magnetic field. Investigated cells were exposed to a static magnetic field (MF) with the intensity of 0.5 T. In order to investigate the effects of magnetic field on stem cell signaling, the localization and density and content of microvesicles (MVs) as well as morphology, ultrastructure, and proliferation rate of equine ASCs were evaluated. Results showed that potential of equine adipose-derived mesenchymal stem cells was accelerated when magnetic field was applied. Resazurin-based assay indicated that the cells cultured in the magnetic field reached the population doubling time earlier and colony-forming potential of equine ASCs was higher when cells were cultured under magnetic field conditions. Morphological and ultrastructural examination of equine ASCs showed that the exposure to magnetic field did not cause any significant changes in cell morphology whereas the polarity of the cells was observed under the magnetic field conditions in ultrastructural examinations. Exposition to MF resulted in a considerable increase in the number of secreted MVs-we have clearly observed the differences between the numbers of MVs shed from the cells cultured under MF in comparison to the control culture and were rich in growth factors. Microvesicles derived from ASCs cultured in the MF condition might be utilized in the stem cell-based treatment of equine musculoskeletal disorders and tendon injuries.
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Publication Date: 2014-11-27 PubMed ID: 25428200PubMed Central: PMC4368852DOI: 10.1007/s11626-014-9828-0Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research study aims to explore the physiological activity of equine adipose-derived stem cells (ASCs) under static magnetic field conditions. The findings reveal an accelerated potential of these stem cells when exposed to a magnetic field, leading to an increase in both the cell doubling time and colony formation. The study also confirms that the magnetic field induces changes in cell polarity and increases the number of secreted microvesicles (MVs) enriched with growth factors, which can potentially be utilized in treating equine musculoskeletal disorders and tendon injuries.

Investigation into Adipose-Derived Stem Cells

  • The main focus of this experimental study was to examine the cellular and physiological activities of equine adipose-derived stem cells (ASCs).
  • These cells were cultured under a static magnetic field with the intensity of 0.5 T. The idea was to understand how these conditions affect the behavior of these stem cells.

Impact of Magnetic Field on ASCs

  • The research findings indicate that the proliferation rate of these ASCs is amplified under the influence of a magnetic field.
  • Using the resazurin-based assay, it was shown that these stem cells cultured in a magnetic field reached the population doubling time earlier than those not exposed to the magnetic field. This suggests that the magnetic field induces the ASCs to divide and multiply more rapidly.
  • Moreover, the colony-forming potential of equine ASCs was found to be higher when cultured under the magnetic field conditions, indicating the field’s positive impact on the production of new colonies.

Morphological and Ultrastructural Examination

  • Morphological and ultrastructural examinations were conducted to observe changes within the cells under the influence of the magnetic field.
  • Although no significant changes in the overall cell morphology were found, clear alterations in cell polarity were observed in ultrastructural inspections under magnetic field conditions.

Microvesicles Secretion

  • The exposure of ASCs to the magnetic field led to a significant increase in the secretion of Microvesicles (MVs). These MVs are small extracellular particles that are secreted by cells and are utilized in intercellular communication and protein transport.
  • The MVs carried important growth factors, indicating that these could be harnessed and used in treating equine musculoskeletal disorders and tendon injuries.

Cite This Article

APA
Marędziak M, Marycz K, Lewandowski D, Siudzińska A, Śmieszek A. (2014). Static magnetic field enhances synthesis and secretion of membrane-derived microvesicles (MVs) rich in VEGF and BMP-2 in equine adipose-derived stromal cells (EqASCs)-a new approach in veterinary regenerative medicine. In Vitro Cell Dev Biol Anim, 51(3), 230-240. https://doi.org/10.1007/s11626-014-9828-0

Publication

In vitro cellular & developmental biology. Animal
ISSN: 1543-706X
NlmUniqueID: 9418515
Country: Germany
Language: English
Volume: 51
Issue: 3
Pages: 230-240

Researcher Affiliations

Marędziak, Monika
  • Electron Microscopy Laboratory, University of Environmental and Life Sciences Wroclaw, Kozuchowska 5b, 51-631, Wroclaw, Poland, monika.maredziak@gmail.com.
Marycz, Krzysztof
    Lewandowski, Daniel
      Siudzińska, Anna
        Śmieszek, Agnieszka

          MeSH Terms

          • Adipose Tissue / cytology
          • Animals
          • Bone Morphogenetic Protein 2 / metabolism
          • Cell Proliferation
          • Cell Shape
          • Cell-Derived Microparticles / metabolism
          • Cell-Derived Microparticles / ultrastructure
          • Colony-Forming Units Assay
          • Enzyme-Linked Immunosorbent Assay
          • Fluorescent Antibody Technique
          • Horses
          • Immunophenotyping
          • Magnetic Fields
          • Phenotype
          • Regenerative Medicine
          • Stem Cells / cytology
          • Stem Cells / metabolism
          • Stem Cells / ultrastructure
          • Stromal Cells / cytology
          • Stromal Cells / metabolism
          • Stromal Cells / ultrastructure
          • Tumor Necrosis Factor-alpha / metabolism
          • Tumor Suppressor Protein p53 / metabolism
          • Vascular Endothelial Growth Factor A / metabolism
          • Veterinary Medicine

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