Strategies for Processing Semen from Subfertile Stallions for Cooled Transport.
Abstract: Subfertility can be a confusing term because some semen of good quality can have reduced fertility following cooled transport if the semen is processed in an improper manner. General procedures aimed at processing stallion semen for cooled transport are well described. An array of factors could exist in reduced fertility of cool-transported semen. This article focuses on centrifugation techniques that can be used to maximize sperm quality of stallions whose semen is intended for cooled transport. Clinical cases are also provided for practical application of techniques.
Copyright © 2016 Elsevier Inc. All rights reserved.
Publication Date: 2016-10-07 PubMed ID: 27726991DOI: 10.1016/j.cveq.2016.07.007Google Scholar: Lookup
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Summary
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This study explores techniques to effectively process the semen of subfertile stallions for cooled transport. The focus is on centrifugation methods to maximize sperm quality and presents clinical cases for practical implementation of these techniques.
Objective Overview
The research work aims at investigating techniques to enhance the processing of semen from subfertile stallions, specifically for cooled transport. The focus largely involves utilizing centrifugation techniques to optimize the quality of sperm. Practical examples via clinical cases serve to illustrate the proposed methods.
Understanding Subfertility in Stallions
- The article begins by discussing the term ‘subfertility’. While it may appear complicated, it essentially refers to instances where semen, despite being of good quality, shows reduced fertility after undergoing cooling transport, primarily due to improper processing.
- General procedures for processing stallion semen destined for cooled transport already exist, but the specific concern here is subfertility. The focus is on a multitude of factors that potentially result in the diminished fertility of cooled, transported semen.
Centrifugation Techniques
- The crux of this research revolves around optimizing centrifugation techniques to improve the sperm quality of stallions’ semen intended for cooled transport.
- Centrifugation is a separation process that uses the centrifugal force to separate particles from a solution based on their size, shape, density, and viscosity. Here, this principle is applied to maximize the quality of the sperm cells among the other components of the seminal fluid.
Clinical Cases
- To ensure the practicality and effectiveness of the suggested techniques, the article incorporates case studies and clinical settings for application.
- These cases serve as practical examples illustrating the real-world usage of the centrifugation procedures for subfertile stallion semen processing. They provide a benchmark for the techniques’ success rates and can aid in the validation of the proposed methods.
Cite This Article
APA
Varner DD.
(2016).
Strategies for Processing Semen from Subfertile Stallions for Cooled Transport.
Vet Clin North Am Equine Pract, 32(3), 547-560.
https://doi.org/10.1016/j.cveq.2016.07.007 Publication
Researcher Affiliations
- Department of Large Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, 500 Raymond Stotzer Parkway, College Station, TX 77843-4475, USA. Electronic address: dvarner@cvm.tamu.edu.
MeSH Terms
- Animals
- Cold Temperature
- Horses / physiology
- Infertility, Male
- Insemination, Artificial
- Male
- Semen / physiology
- Semen Preservation / veterinary
- Specimen Handling
- Sperm Motility / physiology
Citations
This article has been cited 3 times.- Catalán J, Papas M, Trujillo-Rojas L, Blanco-Prieto O, Bonilla-Correal S, Rodríguez-Gil JE, Miró J, Yeste M. Red LED Light Acts on the Mitochondrial Electron Chain of Donkey Sperm and Its Effects Depend on the Time of Exposure to Light. Front Cell Dev Biol 2020;8:588621.
- Catalán J, Papas M, Gacem S, Mateo-Otero Y, Rodríguez-Gil JE, Miró J, Yeste M. Red-Light Irradiation of Horse Spermatozoa Increases Mitochondrial Activity and Motility through Changes in the Motile Sperm Subpopulation Structure. Biology (Basel) 2020 Aug 29;9(9).
- Brito LFC, Linardi RL, Rosales LAS, Balamurugan NS, Hernández-Avilés C, Ramírez-Agámez L. Evaluation of a Chemically Defined, Long-Term Extender for Liquid Storage of Stallion Semen. Reprod Domest Anim 2025 Sep;60(9):e70126.
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