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Home / Equine Research Bank / Vet Microbiol / Streptococcus equi-derived extracellular vesicles as a vacci...
Veterinary microbiology2021; 259; 109165; doi: 10.1016/j.vetmic.2021.109165

Streptococcus equi-derived extracellular vesicles as a vaccine candidate against Streptococcus equi infection.

Abstract: Streptococcus equi subspecies equi is a pathogenic bacterium that causes strangles, a highly contagious respiratory infection in horses and other equines. The limitations of current vaccines against S. equi infection warrants the development of an affordable, safe, and effective vaccine. Because gram-positive extracellular vesicles (EVs) transport various immunogenic antigens, they are attractive vaccine candidates. Here, we purified the EVs of S. equi ATCC 39506 and evaluated them as a vaccine candidate against S. equi infection in mice. As an initial step, comparative proteomic analysis was performed to characterize the functional features of the EVs. Reverse vaccinology and knowledge-based annotations were then used to screen potential vaccine candidates (PVCs) for S. equi ATCC 39506. Finally, 32 PVCs were found to be enriched in the EV fraction, suggesting the usefulness of this fraction as a vaccine. Importantly, a significantly higher survival rate after S. equi infection was detected in mice immunized with S. equi-derived EVs via the intraperitoneal route than in mice immunized with heat-killed bacteria. Of note, immunoprecipitation-mass spectrometry results validated various immunogenic antigens within the EV proteome. In conclusion, our results suggest that S. equi-derived EVs can serve as a vaccine candidate against S. equi infection.
Copyright © 2021 Elsevier B.V. All rights reserved.
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Publication Date: 2021-06-25 PubMed ID: 34225054DOI: 10.1016/j.vetmic.2021.109165Google Scholar: Lookup
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Summary

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The research investigates the potential of using Streptococcus equi-derived extracellular vesicles as a new, more effective vaccine against strangles, a highly contagious respiratory infection in horses.

Background

  • Streptococcus equi is a bacteria responsible for causing strangles, a serious respiratory infection in horses and other equines.
  • Current vaccines against this disease have limitations, leading to the need for a more effective, safe and affordable vaccine.
  • Gram-positive extracellular vesicles (EVs) are noted to carry various immunogenic antigens (substances that can trigger an immune response), making them a potential candidate for vaccine development.

Methodology and Findings

  • The researchers purified extracellular vesicles from a strain of Streptococcus equi (ATCC 39506) and evaluated their effectiveness as a vaccine in mice.
  • An initial step involved a comparative proteomic analysis to understand the functional features of the vesicles.
  • Reverse vaccinology, an approach to the design of vaccines, aided by genetic information, and knowledge-based annotations were applied to screen potential vaccine candidates among the proteins within Streptococcus equi strain.
  • 32 potential vaccine candidates were identified as being enriched in the EV fraction, suggesting the possible application of this extracellular vesicle fraction as a vaccine.
  • In a survival rate analysis, mice immunized with the Streptococcus equi-derived EVs showed higher survival rates after Streptococcus equi infection, as compared to mice immunized with heat-killed bacteria.
  • Immunoprecipitation-mass spectrometry, an analytical chemistry technique used to study protein interactions, validated the presence of various immunogenic antigens within the EV proteome.

Conclusion

  • The study concluded that Streptococcus equi-derived extracellular vesicles show promise as a vaccine candidate against Streptococcus equi infection.

Cite This Article

APA
Lee H, Yun SH, Hyon JY, Lee SY, Yi YS, Choi CW, Jun S, Park EC, Kim SI. (2021). Streptococcus equi-derived extracellular vesicles as a vaccine candidate against Streptococcus equi infection. Vet Microbiol, 259, 109165. https://doi.org/10.1016/j.vetmic.2021.109165

Publication

Veterinary microbiology
ISSN: 1873-2542
NlmUniqueID: 7705469
Country: Netherlands
Language: English
Volume: 259
Pages: 109165
PII: S0378-1135(21)00188-7

Researcher Affiliations

Lee, Hayoung
  • Research Center for Bioconvergence Analysis, Korea Basic Science Institute (KBSI), Ochang, 28119, Republic of Korea; Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology (KRICT), Daejeon, 34114, Republic of Korea; Bio-Analytical Science, University of Science and Technology, Daejeon 34113, Republic of Korea. Electronic address: lhy3221@kbsi.re.kr.
Yun, Sung Ho
  • Center for Research Equipment, Korea Basic Science Institute (KBSI), Ochang, 28119, Republic of Korea.
Hyon, Ju-Yong
  • Research Center for Bioconvergence Analysis, Korea Basic Science Institute (KBSI), Ochang, 28119, Republic of Korea.
Lee, Sang-Yeop
  • Research Center for Bioconvergence Analysis, Korea Basic Science Institute (KBSI), Ochang, 28119, Republic of Korea; Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology (KRICT), Daejeon, 34114, Republic of Korea.
Yi, Yoon-Sun
  • Center for Research Equipment, Korea Basic Science Institute (KBSI), Ochang, 28119, Republic of Korea.
Choi, Chi-Won
  • KBNP Technology Institute, KBNP, INC., Heungan-daero 415, Dongan-Gu, Anyang, Gyeonggi, Republic of Korea.
Jun, Sangmi
  • Center for Research Equipment, Korea Basic Science Institute (KBSI), Ochang, 28119, Republic of Korea.
Park, Edmond Changkyun
  • Research Center for Bioconvergence Analysis, Korea Basic Science Institute (KBSI), Ochang, 28119, Republic of Korea; Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology (KRICT), Daejeon, 34114, Republic of Korea; Bio-Analytical Science, University of Science and Technology, Daejeon 34113, Republic of Korea.
Kim, Seung Il
  • Research Center for Bioconvergence Analysis, Korea Basic Science Institute (KBSI), Ochang, 28119, Republic of Korea; Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology (KRICT), Daejeon, 34114, Republic of Korea; Bio-Analytical Science, University of Science and Technology, Daejeon 34113, Republic of Korea. Electronic address: ksi@kbsi.re.kr.

MeSH Terms

  • Animals
  • Antigens, Bacterial / administration & dosage
  • Antigens, Bacterial / analysis
  • Antigens, Bacterial / immunology
  • Bacterial Proteins / administration & dosage
  • Bacterial Proteins / analysis
  • Bacterial Proteins / immunology
  • Extracellular Vesicles / chemistry
  • Extracellular Vesicles / immunology
  • Female
  • Horse Diseases / microbiology
  • Horse Diseases / prevention & control
  • Horses
  • Immunoprecipitation
  • Mass Spectrometry / methods
  • Mice
  • Mice, Inbred BALB C
  • Proteomics
  • Streptococcal Infections / immunology
  • Streptococcal Infections / prevention & control
  • Streptococcal Vaccines / administration & dosage
  • Streptococcal Vaccines / immunology
  • Streptococcus equi / immunology
  • Vaccination

Citations

This article has been cited 8 times.
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    doi: 10.1007/s12602-023-10085-3pubmed: 37209320google scholar: lookup
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  4. Srivastava P, Kim KS. Membrane Vesicles Derived from Gut Microbiota and Probiotics: Cutting-Edge Therapeutic Approaches for Multidrug-Resistant Superbugs Linked to Neurological Anomalies.. Pharmaceutics 2022 Nov 3;14(11).
    doi: 10.3390/pharmaceutics14112370pubmed: 36365188google scholar: lookup
  5. Fang X, Tanga BM, Bang S, Seo C, Kim H, Saadeldin IM, Lee S, Cho J. Oviduct Epithelial Cell-Derived Extracellular Vesicles Improve Porcine Trophoblast Outgrowth.. Vet Sci 2022 Nov 4;9(11).
    doi: 10.3390/vetsci9110609pubmed: 36356086google scholar: lookup
  6. Xu G, Guo Z, Liu Y, Yang Y, Lin Y, Li C, Huang Y, Fu Q. Gasdermin D protects against Streptococcus equi subsp. zooepidemicus infection through macrophage pyroptosis.. Front Immunol 2022;13:1005925.
    doi: 10.3389/fimmu.2022.1005925pubmed: 36311722google scholar: lookup
  7. Saadeldin IM, Tanga BM, Bang S, Seo C, Koo O, Yun SH, Kim SI, Lee S, Cho J. ROCK Inhibitor (Y-27632) Abolishes the Negative Impacts of miR-155 in the Endometrium-Derived Extracellular Vesicles and Supports Embryo Attachment.. Cells 2022 Oct 10;11(19).
    doi: 10.3390/cells11193178pubmed: 36231141google scholar: lookup
  8. Lee H, Yun SH, Hyon JY, Lee SY, Yi YS, Choi CW, Jun S, Park EC, Kim SI. Dataset of single nucleotide polymorphisms and comprehensive proteomic analysis of Streptococcus equi subsp. equi ATCC 39506.. Data Brief 2021 Oct;38:107402.
    doi: 10.1016/j.dib.2021.107402pubmed: 34621931google scholar: lookup
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