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Home / Equine Research Bank / Infect Immun / Study of the one-step growth curve of equine infectious anem...
Infection and immunity1972; 5(6); 890-895; doi: 10.1128/iai.5.6.890-895.1972

Study of the one-step growth curve of equine infectious anemia virus by immunofluorescence.

Abstract: Primary horse leukocyte cultures were inoculated with 2 or 10 50% tissue culture infective doses (TCID(50)) of equine infectious anemia (EIA) virus per cell, and the titer of cell-associated and fluid-phase virus was determined from 1 to 72 hr postinoculation (PI). Cover slips were collected from 4 to 72 hr PI and stained for EIA viral antigen by the indirect immunofluorescent (FA) technique. Viral replication was detected after a latent period of approximately 18 to 24 hr and reached peak titers of approximately 10(4.5) to 10(6) TCID(50)/0.5 ml from 48 to 72 hr PI. The fluid phase contained 10(1) to 10(2) TCID(50)/0.5 ml more virus than the cells. Viral antigen was first detected by FA from 18 to 24 hr PI. Approximately 75% of the cells contained antigen in their cytoplasm 72 hr PI. The FA technique is a sensitive method for detecting EIA virus in horse leukocyte cultures.
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Publication Date: 1972-06-01 PubMed ID: 4344094PubMed Central: PMC422458DOI: 10.1128/iai.5.6.890-895.1972Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research explored the one-step growth curve of equine infectious anemia (EIA) virus in horse leukocyte cultures, finding that virus replication occurs after a latency period of about 18 to 24 hours and peaks from 48 to 72 hours post-inoculation. The technique of immunofluorescence was found to be effective in detecting EIA virus.

Methodology

  • The researchers used primary horse leukocyte cultures, which were inoculated with two levels of EIA virus (2 or 10 50% tissue culture infective doses (TCID(50)) per cell).
  • Over a period of 72 hours post-inoculation (PI), they collected measurements at regular intervals (1 to 72 hours).
  • Two variables were tracked: the titer of cell-associated and fluid-phase viruses. The ‘titer’ refers to the concentration of the virus.
  • To detect the presence of the EIA virus, the researchers used indirect immunofluorescent (FA) staining. This technique highlights the viral antigen, thereby confirming the presence of the virus.

Findings

  • The research found that viral replication initiated after a latent period of 18 to 24 hours. The latency period refers to a phase in the lifecycle of the virus where it is present in the host cell but is not actively replicating.
  • Peak viral titers were observed between 48 to 72 hours PI, with the highest recorded concentrations between 10(4.5) and 10(6) TCID(50)/0.5 ml.
  • Interestingly, the fluid phase hosted more virus than the cells, with a difference of 10(1) to 10(2) TCID(50)/0.5 ml.
  • The use of FA technique allowed the detection of viral antigen from the period 18 to 24 hours PI, confirming its sensitivity and effectiveness for this purpose.
  • By the end of the study period (72 hours PI), approximately 75% of the cells had viral antigens in their cytoplasm, further testifying to the EIA virus’s successful replication.

Conclusion

  • The researchers concluded that the study provides valuable insights about the lifecycle of the EIA virus in horse leukocyte cultures.
  • The method of FA staining proved to be a sensitive method for tracking the presence and replication of the EIA virus.

Cite This Article

APA
Ushimi C, Henson JB, Gorham JR. (1972). Study of the one-step growth curve of equine infectious anemia virus by immunofluorescence. Infect Immun, 5(6), 890-895. https://doi.org/10.1128/iai.5.6.890-895.1972

Publication

Infection and immunity
ISSN: 0019-9567
NlmUniqueID: 0246127
Country: United States
Language: English
Volume: 5
Issue: 6
Pages: 890-895

Researcher Affiliations

Ushimi, C
    Henson, J B
      Gorham, J R

        MeSH Terms

        • Animals
        • Antigens, Viral / analysis
        • Cells, Cultured
        • Cytopathogenic Effect, Viral
        • Cytoplasm / immunology
        • Equine Infectious Anemia / diagnosis
        • Fluorescent Antibody Technique
        • Horses
        • Infectious Anemia Virus, Equine / growth & development
        • Leukocytes / immunology
        • Leukocytes / microbiology
        • Serologic Tests
        • Time Factors
        • Virus Replication

        References

        This article includes 18 references
        1. Tajima M, Nakajima H, Ito Y. Electron microscopy of equine infectious anemia virus.. J Virol 1969 Oct;4(4):521-7.
          pubmed: 4311413doi: 10.1128/JVI.4.4.521-527.1969google scholar: lookup
        2. Coggins L, Norcross NL. Immunodiffusion reaction in equine infectious anemia.. Cornell Vet 1970 Apr;60(2):330-5.
          pubmed: 4986043
        3. McGuire TC, Van Hoosier GL Jr, Henson JB. The complement-fixation reaction in eguine infectious anemia: demonstration of inhibition by IgG (T).. J Immunol 1971 Dec;107(6):1738-44.
          pubmed: 5000991
        4. Ito Y, Kono Y, Kobayashi K. Electron microscopic observations of equine infectious anemia (EIA) virus in cultivated horse leukocytes. (Brief report).. Arch Gesamte Virusforsch 1969;28(3):411-6.
          pubmed: 4195626doi: 10.1007/BF01240954google scholar: lookup
        5. Kono Y, Kobayashi K. Complement fixation test of equine infectious anemia. I. Specificity of the test.. Natl Inst Anim Health Q (Tokyo) 1966 Winter;6(4):194-203.
          pubmed: 4292231
        6. McGuire TC, Crawford TB, Henson JB. Immunofluorescent localization of equine infectious anemia virus in tissue.. Am J Pathol 1971 Feb;62(2):283-94.
          pubmed: 4322275
        7. Kobayashi K, Kono Y. Propagation and titration of equine infectious anemia virus in horse leukocyte culture.. Natl Inst Anim Health Q (Tokyo) 1967 Spring;7(1):8-20.
          pubmed: 4293214
        8. Kono Y, Yoshino T, Fukanaga Y. Growth characteristics of equine infectious anemia virus in horse leukocyte cultures. Brief report.. Arch Gesamte Virusforsch 1970;30(2):252-6.
          pubmed: 4318580doi: 10.1007/BF01250196google scholar: lookup
        9. Henson JB, McGuire TC. Immunopathology of equine infectious anemia.. Am J Clin Pathol 1971 Sep;56(3):306-13.
          pubmed: 4328671doi: 10.1093/ajcp/56.3.306google scholar: lookup
        10. Nakajima H, Ushimi C. Immunodiffusion studies of purified equine infectious anemia virus.. Infect Immun 1971 Mar;3(3):373-7.
          pubmed: 16557982doi: 10.1128/iai.3.3.373-377.1971google scholar: lookup
        11. Nakajima H, Tanaka S, Ushimi C. Physicochemical studies of equine infectious anemia virus. IV. Determination of the nucleic acid type in the virus.. Arch Gesamte Virusforsch 1970;31(3):273-80.
          pubmed: 4321449doi: 10.1007/BF01253762google scholar: lookup
        12. Ushimi C, Nakajima H, Tanaka S. Demonstration of equine infectious anemia viral antigen by immunofluorescence.. Natl Inst Anim Health Q (Tokyo) 1970 Summer;10(2):90-1.
          pubmed: 4916559
        13. Harter DH, Robenkranz HS, Rose HM. Nucleic acid content of visna virus.. Proc Soc Exp Biol Med 1969 Jul;131(3):927-33.
          pubmed: 4978246doi: 10.3181/00379727-131-34011google scholar: lookup
        14. Nakajima H, Kono Y, Ushimi C. Characterization of precipitating antibody in equine infectious anemia.. J Immunol 1971 Sep;107(3):889-94.
          pubmed: 4328472
        15. Harter DH, Hsu KC, Rose HM. Immunofluorescence and cytochemical studies of visna virus in cell culture.. J Virol 1967 Dec;1(6):1265-70.
          pubmed: 4192872doi: 10.1128/JVI.1.6.1265-1270.1967google scholar: lookup
        16. Crawford TB, McGuire TC, Henson JB. Detection of equine infectious anemia virus in vitro by immunofluorescence.. Arch Gesamte Virusforsch 1971;34(4):332-9.
          pubmed: 4330258doi: 10.1007/BF01242979google scholar: lookup
        17. THORMAR H. The growth cycle of visna virus in monolayer cultures of sheep cells.. Virology 1963 Mar;19:273-8.
          pubmed: 13984957doi: 10.1016/0042-6822(63)90064-3google scholar: lookup
        18. Moore RW, Redmond HE, Katada M, Wallace M. Growth of the equine infectious anemia virus in a continuous-passage horse leukocyte culture.. Am J Vet Res 1970 Sep;31(9):1569-75.
          pubmed: 4319261

        Citations

        This article has been cited 7 times.
        1. Tong M, Yi L, Sun N, Cheng Y, Cao Z, Wang J, Li S, Lin P, Sun Y, Cheng S. Quantitative Analysis of Cellular Proteome Alterations in CDV-Infected Mink Lung Epithelial Cells.. Front Microbiol 2017;8:2564.
          doi: 10.3389/fmicb.2017.02564pubmed: 29312244google scholar: lookup
        2. Cook RF, Leroux C, Cook SJ, Berger SL, Lichtenstein DL, Ghabrial NN, Montelaro RC, Issel CJ. Development and characterization of an in vivo pathogenic molecular clone of equine infectious anemia virus.. J Virol 1998 Feb;72(2):1383-93.
          doi: 10.1128/JVI.72.2.1383-1393.1998pubmed: 9445039google scholar: lookup
        3. Payne SL, Qi XM, Shao H, Dwyer A, Fuller FJ. Disease induction by virus derived from molecular clones of equine infectious anemia virus.. J Virol 1998 Jan;72(1):483-7.
          doi: 10.1128/JVI.72.1.483-487.1998pubmed: 9420249google scholar: lookup
        4. Sellon DC, Fuller FJ, McGuire TC. The immunopathogenesis of equine infectious anemia virus.. Virus Res 1994 May;32(2):111-38.
          doi: 10.1016/0168-1702(94)90038-8pubmed: 8067050google scholar: lookup
        5. Shively MA, Banks KL, Greenlee A, Klevjer-Anderson P. Antigenic stimulation of T lymphocytes in chronic nononcogenic retrovirus infection: equine infectious anemia.. Infect Immun 1982 Apr;36(1):38-46.
          doi: 10.1128/iai.36.1.38-46.1982pubmed: 6281191google scholar: lookup
        6. Rice NR, Lequarre AS, Casey JW, Lahn S, Stephens RM, Edwards J. Viral DNA in horses infected with equine infectious anemia virus.. J Virol 1989 Dec;63(12):5194-200.
          doi: 10.1128/JVI.63.12.5194-5200.1989pubmed: 2555550google scholar: lookup
        7. Whetter L, Archambault D, Perry S, Gazit A, Coggins L, Yaniv A, Clabough D, Dahlberg J, Fuller F, Tronick S. Equine infectious anemia virus derived from a molecular clone persistently infects horses.. J Virol 1990 Dec;64(12):5750-6.
          doi: 10.1128/JVI.64.12.5750-5756.1990pubmed: 2173767google scholar: lookup
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