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Infection and immunity1972; 5(6); 890-895; doi: 10.1128/iai.5.6.890-895.1972

Study of the one-step growth curve of equine infectious anemia virus by immunofluorescence.

Abstract: Primary horse leukocyte cultures were inoculated with 2 or 10 50% tissue culture infective doses (TCID(50)) of equine infectious anemia (EIA) virus per cell, and the titer of cell-associated and fluid-phase virus was determined from 1 to 72 hr postinoculation (PI). Cover slips were collected from 4 to 72 hr PI and stained for EIA viral antigen by the indirect immunofluorescent (FA) technique. Viral replication was detected after a latent period of approximately 18 to 24 hr and reached peak titers of approximately 10(4.5) to 10(6) TCID(50)/0.5 ml from 48 to 72 hr PI. The fluid phase contained 10(1) to 10(2) TCID(50)/0.5 ml more virus than the cells. Viral antigen was first detected by FA from 18 to 24 hr PI. Approximately 75% of the cells contained antigen in their cytoplasm 72 hr PI. The FA technique is a sensitive method for detecting EIA virus in horse leukocyte cultures.
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Publication Date: 1972-06-01 PubMed ID: 4344094PubMed Central: PMC422458DOI: 10.1128/iai.5.6.890-895.1972Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research explored the one-step growth curve of equine infectious anemia (EIA) virus in horse leukocyte cultures, finding that virus replication occurs after a latency period of about 18 to 24 hours and peaks from 48 to 72 hours post-inoculation. The technique of immunofluorescence was found to be effective in detecting EIA virus.

Methodology

  • The researchers used primary horse leukocyte cultures, which were inoculated with two levels of EIA virus (2 or 10 50% tissue culture infective doses (TCID(50)) per cell).
  • Over a period of 72 hours post-inoculation (PI), they collected measurements at regular intervals (1 to 72 hours).
  • Two variables were tracked: the titer of cell-associated and fluid-phase viruses. The ‘titer’ refers to the concentration of the virus.
  • To detect the presence of the EIA virus, the researchers used indirect immunofluorescent (FA) staining. This technique highlights the viral antigen, thereby confirming the presence of the virus.

Findings

  • The research found that viral replication initiated after a latent period of 18 to 24 hours. The latency period refers to a phase in the lifecycle of the virus where it is present in the host cell but is not actively replicating.
  • Peak viral titers were observed between 48 to 72 hours PI, with the highest recorded concentrations between 10(4.5) and 10(6) TCID(50)/0.5 ml.
  • Interestingly, the fluid phase hosted more virus than the cells, with a difference of 10(1) to 10(2) TCID(50)/0.5 ml.
  • The use of FA technique allowed the detection of viral antigen from the period 18 to 24 hours PI, confirming its sensitivity and effectiveness for this purpose.
  • By the end of the study period (72 hours PI), approximately 75% of the cells had viral antigens in their cytoplasm, further testifying to the EIA virus’s successful replication.

Conclusion

  • The researchers concluded that the study provides valuable insights about the lifecycle of the EIA virus in horse leukocyte cultures.
  • The method of FA staining proved to be a sensitive method for tracking the presence and replication of the EIA virus.

Cite This Article

APA
Ushimi C, Henson JB, Gorham JR. (1972). Study of the one-step growth curve of equine infectious anemia virus by immunofluorescence. Infect Immun, 5(6), 890-895. https://doi.org/10.1128/iai.5.6.890-895.1972

Publication

Infection and immunity
ISSN: 0019-9567
NlmUniqueID: 0246127
Country: United States
Language: English
Volume: 5
Issue: 6
Pages: 890-895

Researcher Affiliations

Ushimi, C
    Henson, J B
      Gorham, J R

        MeSH Terms

        • Animals
        • Antigens, Viral / analysis
        • Cells, Cultured
        • Cytopathogenic Effect, Viral
        • Cytoplasm / immunology
        • Equine Infectious Anemia / diagnosis
        • Fluorescent Antibody Technique
        • Horses
        • Infectious Anemia Virus, Equine / growth & development
        • Leukocytes / immunology
        • Leukocytes / microbiology
        • Serologic Tests
        • Time Factors
        • Virus Replication

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        Citations

        This article has been cited 7 times.
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