Successful engraftment of cultured autologous mesenchymal stem cells in a surgically repaired soft palate defect in an adult horse.
Abstract: The objective of this study was to graft autologous mesenchymal stem cells (MSCs) at the site of surgical repair of a soft palate defect in an adult horse in an attempt to improve wound healing and to investigate whether the transplanted MSCs would integrate into the soft palate structure and participate in regeneration. Bone marrow was collected from an adult horse with a full-thickness soft palate defect. The MSCs were isolated, cultured in monolayers, and labeled with 5-bromo-2-desoxymidine (BrdU) and chloromethylbenzamido-DiI-derived (cm-DiI) before transplantation. The soft palate defect was repaired by mandibular symphysiotomy, and the labeled MSCs were injected into the repaired soft palate. Postmortem examination revealed that 90% of the soft palate defect had been sutured. Staining by BrdU and cm-DiI was intense in the soft palate tissue. Labeled MSCs were detected in tissue slices from the injection sites. The cells were organized in a manner similar to that in native soft palate tissue, indicating successful engraftment. L’objectif de cette étude était de greffer des cellules souches mésenchymateuses autologues (MSC) au site de réparation chirurgicale d’un défaut du palais mou chez un cheval adulte afin de tenter d’améliorer la guérison tissulaire et d’étudier si les MSC transplantées s’intégreraient dans la structure du palais mou et participeraient à la régénération. De la moelle osseuse a été prélevée d’un cheval adulte avec un défaut de toute l’épaisseur du palais mou. Les MSC ont été isolées, cultivées en monocouche et marquées avec du 5-bromo-2-désoxymidine (BrdU) et du chlorométhylbenzamido-DiI (cm-DiI) avant la transplantation. Le défaut du palais mou a été réparé par symphyséotomie mandibulaire, et les MSC marquées ont été injectées dans le palais mou réparé. L’examen post-mortem a montré que 90 % du défaut du palais mou avait été suturé. La coloration par BrdU et cm-DiI était intense dans le tissu du palais mou. Des MSC marquées ont été détectées dans des tranches de tissu provenant des sites d’injection. Les cellules étaient organisées d’une manière similaire à celle retrouvée dans du tissu de palais mou original, indiquant ainsi une prise de greffe réussie. (Traduit par Docteur Serge Messier)
Publication Date: PubMed ID: 16639947
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Summary
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The research conducted an experiment to improve wound healing on a horse’s soft palate defect using autologous mesenchymal stem cells (MSCs). Successful integration of the injected MSCs into the soft palate structure was observed, suggesting potential improvements in tissue regeneration.
Introduction
- Scientists attempted to enhance the healing of a surgically repaired soft palate defect in a horse by engrafting cells derived from the horse itself.
- The autologous mesenchymal stem cells (MSCs) investigated in this study represent a type of self-regenerating cell that can transform into a variety of cell types.
- The study utilized MSCs as they are increasingly recognized for their potential role in tissue regeneration and healing, including in soft palate defects.
Method
- Bone marrow containing MSCs was collected from the adult horse which had a full-thickness soft palate defect.
- The harvested MSCs were cultured (grown in a controlled environment) and tagged with markers 5-bromo-2-desoxymidine (BrdU) and chloromethylbenzamido-DiI-derived (cm-DiI) to track them after transplantation.
- A mandibular symphysiotomy, a surgical procedure, was done to repair the soft palate defect. Hereafter, the marked MSCs were directly injected into the repaired soft palate.
Results and interpretation
- Upon postmortem examination, a full recovery of 90% of the soft palate defect was reported. This indicates that the majority of the soft palate wound had healed following surgery and MSCs transplantation.
- Significant BrdU and cm-DiI staining was found in the tissue of the soft palate. This indicates the successful integration of the MSCs into the soft palate structure, as these were the markers previously used to tag the MSCs.
- The identified MSCs in the injection sites were organized similarly to the original native soft palate tissue. This suggests that MSCs could have contributed to tissue regeneration and healing.
Conclusion
- The research provides a foundation for the potential use of autologous MSCs in enhancing wound healing in surgical repairs, particularly in soft palate defects.
- Although the study was conducted on a horse, the results may open further research possibilities for utilizing MSCs in tissue regeneration across different species, including humans.
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Successful engraftment of cultured autologous mesenchymal stem cells in a surgically repaired soft palate defect in an adult horse.
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