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The Journal of physiology1975; 250(3); 633-649; doi: 10.1113/jphysiol.1975.sp011074

Sweat gland function in isolated perfused skin.

Abstract: 1. A technique for perfusion of skin has been used to investigate a possible neurochemical basis for the different patterns of sweating in domestic animals. Evaporative water loss was measured from excised trunk skin, ears or tails perfused with a nutrient Krebs solution, to which drugs were added as required. Perfused skin was observed to sweat in response to administration of sudorific drugs, and some features of the patterns of sweating were similar to those which could be induced by heating or by drugs in conscious animals. 2. In sheep and goat skin, injections of adrenaline, and to a lesser extent of noradrenaline, elicited brief sweat discharges but these were not sustained when the drugs were infused during 10-20 min. Injections of isoprenaline, carbachol, 5-HT, bradykinin, oxytocin and histamine were all ineffective. 3. Injections of adrenaline into cattle skin evoked longer-lasting sweat discharges, and infusions of adrenaline elicited continuous discharges. Injections of noradrenaline and sometimes of bradykinin caused only brief sweat discharges; other drugs were ineffective. 4. In horse and donkey skin, injections or infusions of noradrenaline, oxytocin and bradykinin elicited brief discharges of sweat. Infusions of isoprenaline caused a continuous and profuse outflow of sweat. Infusions of adrenaline also caused a continuous discharge which was usually biphasic in its onset. Other drugs were ineffective. 5. Assuming that the brief sweat discharges are due to myoepithelial contractions and the continuous discharges to sustained increases in secretion, equine sweat glands seem to have a alpha-adrenergically controlled myoepithelium and a beta-adrenergically controlled secretory mechanism. Sheep and goats may have a similar alpha-adrenergic control of the sweat gland myoepithelium but only a feeble sweat secretory mechanism. In cattle, an alpha-adrenergic mechanism appears to control sweat secretion, but the control of the myoepithelium is uncertain.
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Publication Date: 1975-09-01 PubMed ID: 1177153PubMed Central: PMC1348397DOI: 10.1113/jphysiol.1975.sp011074Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article studies the neurochemical basis of the varying patterns of sweating in different animals by using a special perfusion technique on excised skin samples. It investigates the response of these skin samples to various drugs and identifies potential mechanisms controlling sweat gland function.

Research Methodology

  • The study employed a special technique involving perfusion (circulation of a fluid through the blood vessels or other natural channels in an organ or tissue) of skin to unveil a potential neurochemical explanation for the different patterns of sweating in domestic animals.
  • Evaporative water loss was measured from excised skin (removed from the body) of different body parts which were perfused with a nutrient Krebs solution, a commonly used in vitro solution for physiological experiments.
  • Various drugs were added to this solution as required to observe their effects on sweat production.

Experiments and Observations

  • Different drugs elicited different responses in terms of sweat discharges in skin from different animals. For example, adrenaline and noradrenaline stimulated brief sweat discharges in sheep and goats but these were not sustained over time. Isoprenaline, carbachol, 5-HT, bradykinin, oxytocin, and histamine however had no effect.
  • In contrast, adrenaline induced longer-lasting sweat discharges in cattle skin, with continuous discharge upon infusion. Noradrenaline and bradykinin only provoked brief sweat discharge and other drugs were ineffective.
  • In the skin of horses and donkeys, noradrenaline, oxytocin, and bradykinin caused brief sweat discharges while infusions of isoprenaline and adrenaline led to a continuous discharge.

Conclusion

  • Conclusively, it was inferred that the different responses may be due to varying control mechanisms of the sweat glands across species.
  • Equine (horse) sweat glands seem to have an alpha-adrenergically controlled myoepithelium (a thin layer of contractile cells) and a beta-adrenergically controlled secretory mechanism.
  • While in sheep and goats, an alpha-adrenergic control of the sweat gland myoepithelium seems to be present but only a weak sweat secretory mechanism, in comparison.
  • In cattle, an alpha-adrenergic mechanism seems to control sweat secretion, but the control of the myoepithelium is still uncertain.

Cite This Article

APA
Johnson KG. (1975). Sweat gland function in isolated perfused skin. J Physiol, 250(3), 633-649. https://doi.org/10.1113/jphysiol.1975.sp011074

Publication

The Journal of physiology
ISSN: 0022-3751
NlmUniqueID: 0266262
Country: England
Language: English
Volume: 250
Issue: 3
Pages: 633-649

Researcher Affiliations

Johnson, K G

    MeSH Terms

    • Animals
    • Bradykinin / pharmacology
    • Carbachol / pharmacology
    • Cattle
    • Epinephrine / pharmacology
    • Goats
    • Histamine / pharmacology
    • Horses
    • In Vitro Techniques
    • Isoproterenol / pharmacology
    • Norepinephrine / pharmacology
    • Oxytocin / pharmacology
    • Serotonin / pharmacology
    • Sheep
    • Skin Physiological Phenomena
    • Sweat Glands / physiology
    • Sweating / drug effects

    References

    This article includes 14 references
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    Citations

    This article has been cited 4 times.
    1. Balaz S. Modeling kinetics of subcellular disposition of chemicals. Chem Rev 2009 May;109(5):1793-899.
      doi: 10.1021/cr030440jpubmed: 19265398google scholar: lookup
    2. Warner A, Mayhew IG. Equine anhidrosis: a review of pathophysiologic mechanisms. Vet Res Commun 1983 Sep;6(4):249-64.
      doi: 10.1007/BF02214921pubmed: 6359664google scholar: lookup
    3. Brayden DJ, Cuthbert AW, Lee CM. Human eccrine sweat gland epithelial cultures express ductal characteristics. J Physiol 1988 Nov;405:657-75.
      doi: 10.1113/jphysiol.1988.sp017354pubmed: 3255802google scholar: lookup
    4. Jenkinson DM, Montgomery I, Elder HY. Studies on the nature of the peripheral sudomotor control mechanism. J Anat 1978 Mar;125(Pt 3):625-39.
      pubmed: 640964
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