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Home / Equine Research Bank / Stem Cell Rev Rep / Systemic Administration of Rejuvenated Adipose-Derived Mesen...
Stem cell reviews and reports2019; 15(6); 842-850; doi: 10.1007/s12015-019-09913-3

Systemic Administration of Rejuvenated Adipose-Derived Mesenchymal Stem Cells Improves Liver Metabolism in Equine Metabolic Syndrome (EMS)- New Approach in Veterinary Regenerative Medicine.

Abstract: Equine metabolic syndrome (EMS) is characterized by adiposity, insulin dysregulation and increased risk for laminitis. Increased levels of specific liver enzymes in the peripheral blood are typical findings in horses diagnosed with EMS. Current management of EMS is based on caloric restriction and increased physical activity. However, new potential treatment options are arising such as the transplantation of autologous adipose stem cells (ASC). However, cytophysiological properties of ASC derived from EMS horses are impaired which strongly limits their therapeutic potential. We hypothesized, that in vitro pharmacotherapy of those cells with 5-azacytidine (AZA) and resveratrol (RES) before their clinical application can reverse the aged phenotype of those cells and improve clinical outcome of autologous therapy. A 9 year old Dutch Warmblood Horse used for driving, was presented with severe obesity, insulin resistance. After EMS diagnosis, the animal received three intravenous injections of autologous, AZA/RES treated ASCs at weekly intervals. The therapeutic effect was assessed by the analysis of liver specific enzymes in the blood. ASC-transplantation reduced levels of glutamate dehydrogenase (GLDH), gamma-glutamyltransferase (GGT), lactate dehydrogenase (LDH) and aspartate transaminase (AST). This case report demonstrates the therapeutic potential of this intervention for EMS as well as apt utility of autologous, rejuvenated ASC injections.
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Publication Date: 2019-10-18 PubMed ID: 31620992PubMed Central: PMC6925066DOI: 10.1007/s12015-019-09913-3Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

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This study explores the impact of using rejuvenated adipose-derived mesenchymal stem cells to improve liver function and metabolic health in horses with equine metabolic syndrome (EMS), offering a new potential treatment option in veterinary regenerative medicine.

Introduction and Background

  • The research document is about the equine metabolic syndrome (EMS), a condition prevalent in horses, characterized by insulin dysregulation, obesity, and the risk for laminitis (a severe disease affecting a horse’s hooves). An increase in certain liver enzymes in a horse’s peripheral blood is a typical identifier of EMS.
  • Presently, the management of EMS is based on reducing caloric intake and increasing physical activities. However, researchers are exploring new potential treatment options, such as autologous adipose stem cell (ASC) transplantation.
  • The stem cells, ASC, derived from EMS-affected horses have impaired physiological properties, which limit their therapeutic potential.

Research Hypothesis and Method

  • The researchers hypothesized that treating these impaired stem cells with 5-azacytidine (AZA) and resveratrol (RES) in vitro before clinical application could rejuvenate these cells, reversing their aged phenotype and improving the outcome of autologous therapy.
  • To test their hypothesis, they applied this method on a 9-year-old Dutch Warmblood Horse with severe EMS symptoms, including obesity and insulin resistance. They administered three intravenous injections of autologous, AZA/RES-treated ASCs to the subject at weekly intervals.

Results and Conclusion

  • The researchers assessed the treatment’s efficacy by analyzing the levels of liver-specific enzymes in the horse’s blood post-transplantation. The results showed a reduction in the levels of glutamate dehydrogenase (GLDH), gamma-glutamyltransferase (GGT), lactate dehydrogenase (LDH), and aspartate transaminase (AST).
  • This indicated that ASC transplantation improved the metabolic functionality of the liver.
  • The authors concluded that this single case study demonstrated potentially significant therapeutic potential by using this method for treating EMS. Autologous, rejuvenated stem cell injections could be a promising therapeutic and intervention strategy.

Cite This Article

APA
Marycz K, Szłapka-Kosarzewska J, Geburek F, Kornicka-Garbowska K. (2019). Systemic Administration of Rejuvenated Adipose-Derived Mesenchymal Stem Cells Improves Liver Metabolism in Equine Metabolic Syndrome (EMS)- New Approach in Veterinary Regenerative Medicine. Stem Cell Rev Rep, 15(6), 842-850. https://doi.org/10.1007/s12015-019-09913-3

Publication

Stem cell reviews and reports
ISSN: 2629-3277
NlmUniqueID: 101752767
Country: United States
Language: English
Volume: 15
Issue: 6
Pages: 842-850

Researcher Affiliations

Marycz, Krzysztof
  • International Institute of Translational Medicine, Malin, Jesionowa 11 street, 55-114, Wisznia Mała, Poland. krzysztof.marycz@upwr.edu.pl.
  • Department of Experimental Biology, The Faculty of Biology and Animal Science, University of Environmental and Life Sciences Wroclaw, Wroclaw, Poland. krzysztof.marycz@upwr.edu.pl.
  • Faculty of Veterinary Medicine, Clinic for Horses - Department for Surgery, Justus-Liebig-University, Gießen, Germany. krzysztof.marycz@upwr.edu.pl.
Szłapka-Kosarzewska, J
    Geburek, F
    • Faculty of Veterinary Medicine, Clinic for Horses - Department for Surgery, Justus-Liebig-University, Gießen, Germany.
    Kornicka-Garbowska, K
    • International Institute of Translational Medicine, Malin, Jesionowa 11 street, 55-114, Wisznia Mała, Poland.
    • Department of Experimental Biology, The Faculty of Biology and Animal Science, University of Environmental and Life Sciences Wroclaw, Wroclaw, Poland.

    MeSH Terms

    • Animals
    • Antimetabolites, Antineoplastic / pharmacology
    • Azacitidine / pharmacology
    • Horses
    • Insulin Resistance
    • Liver / drug effects
    • Liver / metabolism
    • Liver / pathology
    • Mesenchymal Stem Cell Transplantation / veterinary
    • Mesenchymal Stem Cells / cytology
    • Metabolic Syndrome / etiology
    • Metabolic Syndrome / pathology
    • Metabolic Syndrome / therapy
    • Obesity / complications
    • Regenerative Medicine

    Conflict of Interest Statement

    Authors declare that there is no conflict of interest.

    References

    This article includes 51 references
    1. Frank N. Equine metabolic syndrome.. Journal of Equine Veterinary Science 2009;29(5):259–267.
      doi: 10.1016/j.jevs.2009.04.183google scholar: lookup
    2. Wyse CA, McNie KA, Tannahill VJ, Tannahil VJ, Murray JK, Love S. Prevalence of obesity in riding horses in Scotland.. The Veterinary Record 2008;162(18):590–591.
      doi: 10.1136/vr.162.18.590pubmed: 18453379google scholar: lookup
    3. Stephenson HM, Green MJ, Freeman SL. Prevalence of obesity in a population of horses in the UK.. The Veterinary Record 2011;168(5):131.
      doi: 10.1136/vr.c6281pubmed: 21257596google scholar: lookup
    4. Marycz K, Grzesiak J, Wrzeszcz K, Golonka P. Adipose stem cell combined with plasma-based implant bone tissue differentiation in vitro and in a horse with a phalanx digitalis distalis fracture: A case report.. Veterinarni Medicina (Czech Republic) 2012;57(11):610–617.
      doi: 10.17221/6469-VETMEDgoogle scholar: lookup
    5. Powell DM, Reedy SE, Sessions DR, Fitzgerald BP. Effect of short-term exercise training on insulin sensitivity in obese and lean mares.. Equine Veterinary Journal 2002;34(Supplement):81–84.
      doi: 10.1111/j.2042-3306.2002.tb05396.xpubmed: 12405664google scholar: lookup
    6. Hustace JL, Firshman AM, Mata JE. Pharmacokinetics and bioavailability of metformin in horses.. American Journal of Veterinary Research 2009;70(5):665–668.
      doi: 10.2460/ajvr.70.5.665pubmed: 19405907google scholar: lookup
    7. Wearn JMG, Crisman MV, Davis JL, Geor RJ, Hodgson DR, Suagee JK. Pharmacokinetics of pioglitazone after multiple oral dose administration in horses.. Journal of Veterinary Pharmacology and Therapeutics 2011;34(3):252–258.
      doi: 10.1111/j.1365-2885.2010.01217.xpubmed: 21492190google scholar: lookup
    8. Marycz Krzysztof, Mierzejewska Katarzyna, Śmieszek Agnieszka, Suszynska Ewa, Malicka Iwona, Kucia Magda, Ratajczak Mariusz Z. Endurance Exercise Mobilizes Developmentally Early Stem Cells into Peripheral Blood and Increases Their Number in Bone Marrow: Implications for Tissue Regeneration.. Stem Cells International 2016;2016:1–10.
      doi: 10.1155/2016/5756901pmc: PMC4655293pubmed: 26664409google scholar: lookup
    9. Oreffo ROC, Cooper C, Mason C, Clements M. Mesenchymal stem cells.. Stem Cell Reviews 2005;1(2):169–178.
      doi: 10.1385/SCR:1:2:169pubmed: 17142852google scholar: lookup
    10. Holan V, Hermankova B, Bohacova P, Kossl J, Chudickova M, Hajkova M, Javorkova E. Distinct Immunoregulatory mechanisms in mesenchymal stem cells: Role of the cytokine environment.. Stem Cell Reviews and Reports 2016;12(6):654–663.
      doi: 10.1007/s12015-016-9688-ypubmed: 27665290google scholar: lookup
    11. Abumaree M, Al Jumah M, Pace RA, Kalionis B. Immunosuppressive properties of mesenchymal stem cells.. Stem Cell Reviews and Reports 2012;8(2):375–392.
      doi: 10.1007/s12015-011-9312-0pubmed: 21892603google scholar: lookup
    12. Cislo-Pakuluk A, Marycz K. A promising tool in retina regeneration: Current perspectives and challenges when using mesenchymal progenitor stem cells in veterinary and human ophthalmological applications.. Stem Cell Reviews 2017;13(5):598–602.
      doi: 10.1007/s12015-017-9750-4pmc: PMC5602072pubmed: 28643176google scholar: lookup
    13. Wang H, Zhang H, Huang B, Miao G, Yan X, Gao G, Yang L. Mesenchymal stem cells reverse high-fat diet-induced non-alcoholic fatty liver disease through suppression of CD4+ T lymphocytes in mice.. Molecular Medicine Reports 2018;17(3):3769–3774.
      doi: 10.3892/mmr.2017.8326pubmed: 29286155google scholar: lookup
    14. Tsuchiya A, Kojima Y, Ikarashi S, Seino S, Watanabe Y, Kawata Y, Terai S. Clinical trials using mesenchymal stem cells in liver diseases and inflammatory bowel diseases.. Inflammation and Regeneration 2017;37:16.
      doi: 10.1186/s41232-017-0045-6pmc: PMC5725741pubmed: 29259715google scholar: lookup
    15. Marycz K, Michalak I, Kornicka K. Advanced nutritional and stem cells approaches to prevent equine metabolic syndrome.. Research in Veterinary Science 2018;118:115–125.
      doi: 10.1016/j.rvsc.2018.01.015pubmed: 29421480google scholar: lookup
    16. da Justa Pinheiro CH, de Queiroz JCF, Guimarães-Ferreira L, Vitzel KF, Nachbar RT, de Sousa LGO, Curi R. Local injections of adipose-derived mesenchymal stem cells modulate inflammation and increase angiogenesis ameliorating the dystrophic phenotype in dystrophin-deficient skeletal muscle.. Stem Cell Reviews and Reports 2012;8(2):363–374.
      doi: 10.1007/s12015-011-9304-0pubmed: 21874281google scholar: lookup
    17. Fiore EJ, Mazzolini G, Aquino JB. Mesenchymal stem/stromal cells in liver fibrosis: Recent findings, old/new caveats and future perspectives.. Stem Cell Reviews and Reports 2015;11(4):586–597.
      doi: 10.1007/s12015-015-9585-9pubmed: 25820543google scholar: lookup
    18. Marycz K, Kornicka K, Marędziak M, Golonka P, Nicpoń J. Equine metabolic syndrome impairs adipose stem cells osteogenic differentiation by predominance of autophagy over selective mitophagy.. Journal of Cellular and Molecular Medicine 2016;20(12):2384–2404.
      doi: 10.1111/jcmm.12932pmc: PMC5134411pubmed: 27629697google scholar: lookup
    19. Marycz K, Kornicka K, Basinska K, Czyrek A. Equine metabolic syndrome affects viability, senescence, and stress factors of equine adipose-derived mesenchymal stromal stem cells: New insight into EqASCs Isolated from EMS horses in the context of their aging.. Oxidative Medicine and Cellular Longevity 2016;2016:1–17.
      doi: 10.1155/2016/4710326pmc: PMC4670679pubmed: 26682006google scholar: lookup
    20. Kornicka Katarzyna, Houston Jenny, Marycz Krzysztof. Dysfunction of Mesenchymal Stem Cells Isolated from Metabolic Syndrome and Type 2 Diabetic Patients as Result of Oxidative Stress and Autophagy may Limit Their Potential Therapeutic Use.. Stem Cell Reviews and Reports 2018;14(3):337–345.
      doi: 10.1007/s12015-018-9809-xpmc: PMC5960487pubmed: 29611042google scholar: lookup
    21. Pezzanite LM, Fortier LA, Antczak DF, Cassano JM, Brosnahan MM, Miller D, Schnabel LV. Equine allogeneic bone marrow-derived mesenchymal stromal cells elicit antibody responses in vivo.. Stem Cell Research & Therapy 2015;6:54.
      doi: 10.1186/s13287-015-0053-xpmc: PMC4414005pubmed: 25889095google scholar: lookup
    22. Owens SD, Kol A, Walker NJ, Borjesson DL. Allogeneic mesenchymal stem cell treatment induces specific alloantibodies in horses.. Stem Cells International 2016;2016:1–8.
      doi: 10.1155/2016/5830103pmc: PMC5018342pubmed: 27648075google scholar: lookup
    23. Kornicka K, Marycz K, Marędziak M, Tomaszewski KA, Nicpoń J. The effects of the DNA methyltranfserases inhibitor 5-Azacitidine on ageing, oxidative stress and DNA methylation of adipose derived stem cells.. Journal of Cellular and Molecular Medicine 2017;21(2):387–401.
      doi: 10.1111/jcmm.12972pmc: PMC5264131pubmed: 27998022google scholar: lookup
    24. Yan X, Ehnert S, Culmes M, Bachmann A, Seeliger C, Schyschka L, Nussler AK. 5-azacytidine improves the osteogenic differentiation potential of aged human adipose-derived mesenchymal stem cells by DNA demethylation.. PLoS One 2014;9(6):e90846.
      doi: 10.1371/journal.pone.0090846pmc: PMC3946260pubmed: 24603866google scholar: lookup
    25. Švajger U, Jeras M. Anti-inflammatory effects of resveratrol and its potential use in therapy of immune-mediated diseases.. International Reviews of Immunology 2012;31(3):202–222.
      doi: 10.3109/08830185.2012.665108pubmed: 22587021google scholar: lookup
    26. Gülçin İ. Antioxidant properties of resveratrol: A structure–activity insight.. Innovative Food Science & Emerging Technologies 2010;11(1):210–218.
      doi: 10.1016/j.ifset.2009.07.002google scholar: lookup
    27. Aguirre L, Fernández-Quintela A, Arias N, Portillo MP. Resveratrol: Anti-obesity mechanisms of action.. Molecules (Basel, Switzerland) 2014;19(11):18632–18655.
      doi: 10.3390/molecules191118632pmc: PMC6271102pubmed: 25405284google scholar: lookup
    28. Bhullar KS, Hubbard BP. Lifespan and healthspan extension by resveratrol.. Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease 2015;1852(6):1209–1218.
      doi: 10.1016/j.bbadis.2015.01.012pubmed: 25640851google scholar: lookup
    29. Kornicka K, Szłapka-Kosarzewska J, Śmieszek A, Marycz K. 5-Azacytydine and resveratrol reverse senescence and ageing of adipose stem cells via modulation of mitochondrial dynamics and autophagy.. Journal of Cellular and Molecular Medicine 2019;23(1):237–259.
      doi: 10.1111/jcmm.13914pmc: PMC6307768pubmed: 30370650google scholar: lookup
    30. Kornicka Katarzyna, Śmieszek Agnieszka, Węgrzyn Agnieszka, Röcken Michael, Marycz Krzysztof. Immunomodulatory Properties of Adipose-Derived Stem Cells Treated with 5-Azacytydine and Resveratrol on Peripheral Blood Mononuclear Cells and Macrophages in Metabolic Syndrome Animals.. Journal of Clinical Medicine 2018;7(11):383.
      doi: 10.3390/jcm7110383pmc: PMC6262510pubmed: 30356025google scholar: lookup
    31. Kosolofski HR, Gow SP, Robinson KA. Prevalence of obesity in the equine population of Saskatoon and surrounding area.. The Canadian Veterinary Journal 2017;58(9):967.
      pmc: PMC5556474pubmed: 28878421
    32. Carter RA, Geor RJ, Burton Staniar W, Cubitt TA, Harris PA. Apparent adiposity assessed by standardised scoring systems and morphometric measurements in horses and ponies.. Veterinary Journal 2009;179(2):204–210.
      doi: 10.1016/j.tvjl.2008.02.029pubmed: 18440844google scholar: lookup
    33. Frank N, Walsh DM. Repeatability of Oral sugar test results, glucagon-like Peptide-1 measurements, and serum high-molecular-weight adiponectin concentrations in horses.. Journal of Veterinary Internal Medicine 2017;31(4):1178–1187.
      doi: 10.1111/jvim.14725pmc: PMC5508339pubmed: 28543933google scholar: lookup
    34. Marędziak M, Marycz K, Lewandowski D, Siudzińska A, Śmieszek A. Static magnetic field enhances synthesis and secretion of membrane-derived microvesicles (MVs) rich in VEGF and BMP-2 in equine adipose-derived stromal cells (EqASCs)-a new approach in veterinary regenerative medicine.. In Vitro Cellular & Developmental Biology Animal 2015;51(3):230–240.
      doi: 10.1007/s11626-014-9828-0pmc: PMC4368852pubmed: 25428200google scholar: lookup
    35. Marycz K, Kornicka K, Irwin-Houston JM, Weiss C. Combination of resveratrol and 5-azacytydine improves osteogenesis of metabolic syndrome mesenchymal stem cells.. Journal of Cellular and Molecular Medicine 2018;22(10):4771–4793.
      doi: 10.1111/jcmm.13731pmc: PMC6156237pubmed: 29999247google scholar: lookup
    36. Si Y, Zhao Y, Hao H, Liu J, Guo Y, Mu Y, Han W. Infusion of mesenchymal stem cells ameliorates hyperglycemia in type 2 diabetic rats: Identification of a novel role in improving insulin sensitivity.. Diabetes 2012;61(6):1616–1625.
      doi: 10.2337/db11-1141pmc: PMC3357293pubmed: 22618776google scholar: lookup
    37. Shree N, Bhonde RR. Conditioned media from adipose tissue derived mesenchymal stem cells reverse insulin resistance in cellular models.. Journal of Cellular Biochemistry 2017;118(8):2037–2043.
      doi: 10.1002/jcb.25777pubmed: 27791278google scholar: lookup
    38. Xie Z, Hao H, Tong C, Cheng Y, Liu J, Pang Y, Han W. Human umbilical cord-derived mesenchymal stem cells elicit macrophages into an anti-inflammatory phenotype to alleviate insulin resistance in type 2 diabetic rats.. Stem Cells (Dayton, Ohio) 2016;34(3):627–639.
      doi: 10.1002/stem.2238pubmed: 26523620google scholar: lookup
    39. Melief SM, Schrama E, Brugman MH, Tiemessen MM, Hoogduijn MJ, Fibbe WE, Roelofs H. Multipotent stromal cells induce human regulatory T cells through a novel pathway involving skewing of monocytes toward anti-inflammatory macrophages.. Stem Cells (Dayton, Ohio) 2013;31(9):1980–1991.
      doi: 10.1002/stem.1432pubmed: 23712682google scholar: lookup
    40. Madrigal M, Rao KS, Riordan NH. A review of therapeutic effects of mesenchymal stem cell secretions and induction of secretory modification by different culture methods.. Journal of Translational Medicine 2014;12:260.
      doi: 10.1186/s12967-014-0260-8pmc: PMC4197270pubmed: 25304688google scholar: lookup
    41. Zheng S, Yang J, Yang J, Tang Y, Shao Q, Guo L, Liu Q. Transplantation of umbilical cord mesenchymal stem cells via different routes in rats with acute liver failure.. International Journal of Clinical and Experimental Pathology 2015;8(12):15854–15862.
      pmc: PMC4730069pubmed: 26884856
    42. Huang B, Cheng X, Wang H, Huang W, la Ga Hu Z, Wang D, Zhang R. Mesenchymal stem cells and their secreted molecules predominantly ameliorate fulminant hepatic failure and chronic liver fibrosis in mice respectively.. Journal of Translational Medicine 2016;14:45.
      doi: 10.1186/s12967-016-0792-1pmc: PMC4746907pubmed: 26861623google scholar: lookup
    43. Lee RH, Pulin AA, Seo MJ, Kota DJ, Ylostalo J, Larson BL, Prockop DJ. Intravenous hMSCs improve myocardial infarction in mice because cells embolized in lung are activated to secrete the anti-inflammatory protein TSG-6.. Cell Stem Cell 2009;5(1):54–63.
      doi: 10.1016/j.stem.2009.05.003pmc: PMC4154377pubmed: 19570514google scholar: lookup
    44. Eggenhofer E, Benseler V, Kroemer A, Popp FC, Geissler EK, Schlitt HJ, Hoogduijn MJ. Mesenchymal stem cells are short-lived and do not migrate beyond the lungs after intravenous infusion.. Frontiers in Immunology 2012;3:297.
      doi: 10.3389/fimmu.2012.00297pmc: PMC3458305pubmed: 23056000google scholar: lookup
    45. Kim G, Eom YW, Baik SK, Shin Y, Lim YL, Kim MY, Chang SJ. Therapeutic effects of mesenchymal stem cells for patients with chronic liver diseases: Systematic review and meta-analysis.. Journal of Korean Medical Science 2015;30(10):1405–1415.
      doi: 10.3346/jkms.2015.30.10.1405pmc: PMC4575928pubmed: 26425036google scholar: lookup
    46. Wang L, Li J, Liu H, Li Y, Fu J, Sun Y, Wang F-S. Pilot study of umbilical cord-derived mesenchymal stem cell transfusion in patients with primary biliary cirrhosis.. Journal of Gastroenterology and Hepatology 2013;28(Suppl 1):85–92.
      doi: 10.1111/jgh.12029pubmed: 23855301google scholar: lookup
    47. Nam A, Han S-M, Go D-M, Kim D-Y, Seo K-W, Youn H-Y. Long-Term Management with Adipose Tissue-Derived Mesenchymal Stem Cells and Conventional Treatment in a Dog with Hepatocutaneous Syndrome.. Journal of Veterinary Internal Medicine 2017;31(5):1514–1519.
      doi: 10.1111/jvim.14798pmc: PMC5598886pubmed: 28782844google scholar: lookup
    48. Liu Z, Meng F, Li C, Zhou X, Zeng X, He Y, Li T. Human umbilical cord mesenchymal stromal cells rescue mice from acetaminophen-induced acute liver failure.. Cytotherapy 2014;16(9):1207–1219.
      doi: 10.1016/j.jcyt.2014.05.018pubmed: 25108650google scholar: lookup
    49. Lindroos B, Suuronen R, Miettinen S. The potential of adipose stem cells in regenerative medicine.. Stem Cell Reviews 2011;7(2):269–291.
      doi: 10.1007/s12015-010-9193-7pubmed: 20853072google scholar: lookup
    50. Bourebaba L, Röcken M, Marycz K. Osteochondritis dissecans (OCD) in horses – Molecular background of its pathogenesis and perspectives for progenitor stem cell therapy.. Stem Cell Reviews and Reports 2019;15:374–390.
      doi: 10.1007/s12015-019-09875-6pmc: PMC6534522pubmed: 30796679google scholar: lookup
    51. Piryaei A, Valojerdi MR, Shahsavani M, Baharvand H. Differentiation of bone marrow-derived mesenchymal stem cells into hepatocyte-like cells on nanofibers and their transplantation into a carbon tetrachloride-induced liver fibrosis model.. Stem Cell Reviews and Reports 2011;7(1):103–118.
      doi: 10.1007/s12015-010-9126-5pubmed: 20182823google scholar: lookup
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