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Home / Equine Research Bank / Animals (Basel) / Tandem Mass Tag (TMT) Proteomic Analysis of Saliva in Horses...
Animals : an open access journal from MDPI2021; 11(5); 1304; doi: 10.3390/ani11051304

Tandem Mass Tag (TMT) Proteomic Analysis of Saliva in Horses with Acute Abdominal Disease.

Abstract: The aim of this study was to investigate the changes in the salivary proteome in horses with acute abdominal disease (AAD) using a tandem mass tags (TMT)-based proteomic approach. The saliva samples from eight horses with AAD were compared with six healthy horses in the proteomic study. Additionally, saliva samples from eight horses with AAD and eight controls were used to validate lactoferrin (LF) in saliva. The TMT analysis quantified 118 proteins. Of these, 17 differed significantly between horses with AAD and the healthy controls, 11 being downregulated and 6 upregulated. Our results showed the downregulation of gamma-enteric smooth muscle actin (ACTA2), latherin isoform X1, and LF. These proteins could be closely related to an impaired primary immune defense and antimicrobial capacity in the mucosa. In addition, there was an upregulation of mucin 19 (MUC19) and the serine protease inhibitor Kazal-type 5 (SPINK5) associated with a protective effect during inflammation. The proteins identified in our study could have the potential to be novel biomarkers for diagnosis or monitoring the physiopathology of the disease, especially LF, which decreased in the saliva of horses with AAD and was successfully measured using a commercially available immunoassay.
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Publication Date: 2021-04-30 PubMed ID: 33946607PubMed Central: PMC8147179DOI: 10.3390/ani11051304Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article discusses a study that examined changes in the proteins present in the saliva of horses suffering from acute abdominal disease, using a technique called Tandem Mass Tag proteomic analysis. The identified proteins could potentially serve as new markers for diagnosis or disease tracking.

Objective of the Study

  • The primary goal of this research was to study the alterations in the saliva proteome (complete set of proteins expressed by a genome) of horses suffering from acute abdominal disease (AAD) using a Tandem Mass Tag (TMT)-based proteomic approach.

Methodology

  • Saliva samples from eight horses with AAD were compared with the samples from six healthy horses using TMT proteomic analysis.
  • Additional saliva samples from eight horses with AAD and another eight healthy horses were used to confirm the presence of lactoferrin (LF), a protein known for its immune regulatory properties.

Findings

  • The TMT analysis enabled the quantification of 118 proteins in the samples. This yielded 17 significant differences between the AAD horses and their healthy counterparts, with 11 proteins found to be decreased (downregulated) and 6 proteins increased (upregulated) in the AAD horses.
  • The downregulated proteins included gamma-enteric smooth muscle actin (ACTA2), latherin isoform X1, and lactoferrin (LF). These proteins may be linked to decreased primary immune defense and antimicrobial activity in the mucosa — the mucous membranes lining the digestive tract.
  • In contrast, there was an increase in the levels of mucin 19 (MUC19) and serine protease inhibitor Kazal-type 5 (SPINK5) associated with protective effects against inflammation.

Potential Implications

  • The proteins identified in this study could serve as novel biomarkers for diagnosing or monitoring the development of AAD, especially LF. This protein was found to decrease in the saliva of horses with AAD and could be measured using a commercially available immunoassay test.

Cite This Article

APA
Muñoz-Prieto A, Escribano D, Contreras-Aguilar MD, Horvatić A, Guillemin N, Jacobsen S, Cerón JJ, Mrljak V. (2021). Tandem Mass Tag (TMT) Proteomic Analysis of Saliva in Horses with Acute Abdominal Disease. Animals (Basel), 11(5), 1304. https://doi.org/10.3390/ani11051304

Publication

Animals : an open access journal from MDPI
ISSN: 2076-2615
NlmUniqueID: 101635614
Country: Switzerland
Language: English
Volume: 11
Issue: 5
PII: 1304

Researcher Affiliations

Muñoz-Prieto, Alberto
  • Clinic for Internal Diseases, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10 000 Zagreb, Croatia.
Escribano, Damián
  • Interdisciplinary Laboratory of Clinical Analysis, Interlab-UMU, Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, 30100 Murcia, Spain.
Contreras-Aguilar, María Dolores
  • Interdisciplinary Laboratory of Clinical Analysis, Interlab-UMU, Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, 30100 Murcia, Spain.
Horvatić, Anita
  • Department of Chemistry and Biochemistry, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10 000 Zagreb, Croatia.
Guillemin, Nicolas
  • Clinic for Internal Diseases, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10 000 Zagreb, Croatia.
Jacobsen, Stine
  • Department of Veterinary Clinical Sciences, Veterinary School of Medicine, Sektion Medicine and Surgery, University of Copenhagen, Hoejbakkegaard Allé 5, DK-2630 Taastrup, Denmark.
Cerón, José Joaquín
  • Interdisciplinary Laboratory of Clinical Analysis, Interlab-UMU, Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, 30100 Murcia, Spain.
Mrljak, Vladimir
  • Clinic for Internal Diseases, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10 000 Zagreb, Croatia.

Grant Funding

  • 19894/GERM/15 / Fundaciu00f3n Su00e9neca
  • 21239/PD/19 / Fundaciu00f3n Su00e9neca
  • R-1437/2019 / Universidad de Murcia
  • R-27/2020 / Universidad de Murcia
  • R-605/2016 / Universidad de Murcia
  • KK.01.1.16.0004 / European Structural and Investment Funds

Conflict of Interest Statement

The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results.

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Citations

This article has been cited 4 times.
  1. Muñoz-Prieto A, Contreras-Aguilar MD, Cerón JJ, Ayala de la Peña I, Martín-Cuervo M, Eckersall PD, Holm Henriksen IM, Tecles F, Hansen S. Changes in Calprotectin (S100A8-A9) and Aldolase in the Saliva of Horses with Equine Gastric Ulcer Syndrome. Animals (Basel) 2023 Apr 16;13(8).
    doi: 10.3390/ani13081367pubmed: 37106929google scholar: lookup
  2. López-Martínez MJ, Beletić A, Kuleš J, Rešetar-Maslov D, Rubić I, Mrljak V, Manzanilla EG, Goyena E, Martínez-Subiela S, Cerón JJ, Muñoz-Prieto A. Revealing the Changes in Saliva and Serum Proteins of Pigs with Meningitis Caused by Streptococcus Suis: A Proteomic Approach. Int J Mol Sci 2022 Nov 8;23(22).
    doi: 10.3390/ijms232213700pubmed: 36430174google scholar: lookup
  3. Muñoz-Prieto A, Contreras-Aguilar MD, Cerón JJ, Ayala I, Martin-Cuervo M, Gonzalez-Sanchez JC, Jacobsen S, Kuleš J, Beletić A, Rubić I, Mrljak V, Tecles F, Hansen S. Changes in Proteins in Saliva and Serum in Equine Gastric Ulcer Syndrome Using a Proteomic Approach. Animals (Basel) 2022 May 2;12(9).
    doi: 10.3390/ani12091169pubmed: 35565595google scholar: lookup
  4. Bishop RC, Arrington JV, Wilkins PA, McCoy AM. Alterations in the Peritoneal Fluid Proteome of Horses with Colic Attributed to Ischemic and Non-Ischemic Intestinal Disease. Animals (Basel) 2025 May 30;15(11).
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