Ten cases of Mycobacterium avium subsp. hominissuis infections linked to equine abortions in Japan, 2018-2019.
Abstract: Bacterial placentitis in horses commonly results in abortion, premature birth or compromised neonatal foal health. Although mycobacterial infections are generally uncommon in horses, 10 equine abortion cases caused by Mycobacterium avium subsp. hominissuis (MAH) infections occurred between 2018 and 2019 in Japan. They occurred on seven Thoroughbred horse farms in the Hidaka district of Hokkaido, but direct contact among the mares on different farms was not recorded. Most cases were characterized by extensive pathological lesions of the placenta, which are not typical in cases of common pathogenic bacteria such as Streptococcus zooepidemicus and Escherichia coli. All abortions featured white-yellow exudates on the surface of the placenta. Mycobacterial granuloma formations were histologically found in the placenta and fetal organs, and acid-fast bacteria were isolated from the placenta, fetal samples (heart, lung, liver, kidney, spleen and stomach contents) or uterine lavage fluid. The greatest number of bacteria was isolated from necrotic lesions on the placenta, which could be an important site for bacterial isolation in mycobacterial equine abortions. The isolates were identified as MAH based on internal genome sequences. In variable number tandem repeat analysis, all patterns of the strains were identical. Single nucleotide polymorphism analysis of the core genome grouped all strains in the II-a/SC3 subcluster. Both results reveal that these strains share the same genetic background, suggesting that the horses had been infected by the same unknown contagious source.
© 2020 The Authors. Veterinary Medicine and Science Published by John Wiley & Sons Ltd.
Publication Date: 2020-12-18 PubMed ID: 33336899PubMed Central: PMC8136959DOI: 10.1002/vms3.411Google Scholar: Lookup
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Summary
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The research article discusses ten cases of equine abortion in Japan caused by a bacterial infection, specifically Mycobacterium avium subsp. hominissuis (MAH), which occurred between 2018 and 2019 on seven Thoroughbred horse farms.
Background
- Infections caused by Mycobacterium avium subsp. hominissuis (MAH) are generally uncommon in horses, but in this study, 10 instances of equine abortions linked to this bacterial infection were reported in Japan.
- The affected horses were from seven different Thoroughbred farms in the Hidaka district of Hokkaido. However, there were no records of direct contact among the mares in different farms.
Characteristics of the Infections
- Most of the recorded cases featured extensive pathological lesions on the placenta, which is not a common symptom in bacterial infections caused by more common pathogens such as Streptococcus zooepidemicus and Escherichia coli.
- All the abortion cases were noted for featuring white-yellow exudates on the placenta’s surface.
- Mycobacterial granuloma formations were found in the placenta and the fetal organs upon histological examination.
- The bacteria were mainly isolated from the placenta, fetal samples (heart, lung, liver, kidney, spleen, and stomach contents), or uterine lavage fluid.
- The highest number of bacteria was isolated from necrotic lesions on the placenta, which the study suggests could be a critical site for bacterial isolation in mycobacterial equine abortions.
Bacterial Identification and Analysis
- The isolated bacteria were identified as MAH based on internal genome sequences.
- A variable number tandem repeat analysis showed that all patterns of the strains were identical, implying they originated from the same genetic background.
- A single nucleotide polymorphism analysis of the core genome grouped all the strains under the II-a/SC3 subcluster, reaffirming that they shared the same genetic background.
- These results contribute to the hypothesis that horses had been infected by the same, as of yet, unidentified contagious source.
Cite This Article
APA
Kinoshita Y, Takechi M, Uchida-Fujii E, Miyazawa K, Nukada T, Niwa H.
(2020).
Ten cases of Mycobacterium avium subsp. hominissuis infections linked to equine abortions in Japan, 2018-2019.
Vet Med Sci, 7(3), 621-625.
https://doi.org/10.1002/vms3.411 Publication
Researcher Affiliations
- Microbiology Division, Equine Research Institute, Japan Racing Association, Shimotsuke, Japan.
- Hokkaido Hidaka Livestock Hygiene Service Center, Hidakagunshinhidakacho, Japan.
- Microbiology Division, Equine Research Institute, Japan Racing Association, Shimotsuke, Japan.
- Hokkaido Hidaka Livestock Hygiene Service Center, Hidakagunshinhidakacho, Japan.
- Microbiology Division, Equine Research Institute, Japan Racing Association, Shimotsuke, Japan.
- Microbiology Division, Equine Research Institute, Japan Racing Association, Shimotsuke, Japan.
MeSH Terms
- Abortion, Veterinary / microbiology
- Animals
- Horse Diseases / microbiology
- Horses
- Japan
- Mycobacterium / physiology
- Mycobacterium Infections / microbiology
- Mycobacterium Infections / veterinary
Conflict of Interest Statement
The authors declare no conflict of interest.
References
This article includes 22 references
- Bażanów BA, Frącka AB, Jackulak NA, Staroniewicz ZM, Ploch SM. A 34-year retrospective study of equine viral abortion in Poland.. Pol J Vet Sci 2014;17(4):607-12.
- Bouillaut L, McBride SM, Sorg JA. Genetic manipulation of Clostridium difficile.. Curr Protoc Microbiol 2011 Feb;Chapter 9:Unit 9A.2.
- Campora L, Corazza M, Zullino C, Ebani VV, Abramo F. Mycobacterium avium subspecies hominissuis disseminated infection in a Basset Hound dog.. J Vet Diagn Invest 2011 Sep;23(5):1083-7.
- Chae H, Han SJ, Kim SY, Ki CS, Huh HJ, Yong D, Koh WJ, Shin SJ. Development of a One-Step Multiplex PCR Assay for Differential Detection of Major Mycobacterium Species.. J Clin Microbiol 2017 Sep;55(9):2736-2751.
- Cline JM, Schlafer DW, Callihan DR, Vanderwall D, Drazek FJ. Abortion and granulomatous colitis due to Mycobacterium avium complex infection in a horse.. Vet Pathol 1991 Jan;28(1):89-91.
- Giles RC, Donahue JM, Hong CB, Tuttle PA, Petrites-Murphy MB, Poonacha KB, Roberts AW, Tramontin RR, Smith B, Swerczek TW. Causes of abortion, stillbirth, and perinatal death in horses: 3,527 cases (1986-1991).. J Am Vet Med Assoc 1993 Oct 15;203(8):1170-5.
- Hélie P, Higgins R. Mycobacterium avium complex abortion in a mare.. J Vet Diagn Invest 1996 Apr;8(2):257-8.
- Inagaki T, Nishimori K, Yagi T, Ichikawa K, Moriyama M, Nakagawa T, Shibayama T, Uchiya K, Nikai T, Ogawa K. Comparison of a variable-number tandem-repeat (VNTR) method for typing Mycobacterium avium with mycobacterial interspersed repetitive-unit-VNTR and IS1245 restriction fragment length polymorphism typing.. J Clin Microbiol 2009 Jul;47(7):2156-64.
- Johnson AK, Roberts JF, Hagan A, Wilborn RR, Dujovne G, Sells SF, Donahue JM. Infection of an equine placenta with a novel mycobacterial species leading to abortion.. J Vet Diagn Invest 2012 Jul;24(4):785-90.
- Joshi N, Fass J. A sliding‐window, adaptive, quality‐based trimming tool for FastQ files. .
- Kumar S, Stecher G, Li M, Knyaz C, Tamura K. MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms.. Mol Biol Evol 2018 Jun 1;35(6):1547-1549.
- LeBlanc MM. Ascending placentitis in the mare: an update.. Reprod Domest Anim 2010 Jun;45 Suppl 2:28-34.
- Mijs W, de Haas P, Rossau R, Van der Laan T, Rigouts L, Portaels F, van Soolingen D. Molecular evidence to support a proposal to reserve the designation Mycobacterium avium subsp. avium for bird-type isolates and 'M. avium subsp. hominissuis' for the human/porcine type of M. avium.. Int J Syst Evol Microbiol 2002 Sep;52(Pt 5):1505-1518.
- Murase H, Miyazawa M, Harada T, Ozawa M, Sato F, Hada T. Aborted fetal sizes of Thoroughbred horses in Hidaka, Japan, between 2005 and 2015.. J Equine Sci 2017;28(2):47-53.
- Nemoto M, Tsujimura K, Yamanaka T, Kondo T, Matsumura T. Loop-mediated isothermal amplification assays for detection of Equid herpesvirus 1 and 4 and differentiating a gene-deleted candidate vaccine strain from wild-type Equid herpesvirus 1 strains.. J Vet Diagn Invest 2010 Jan;22(1):30-6.
- Pavlik I, Jahn P, Dvorska L, Bartos M, Novorny L, Halouzka R. Mycobacterial infections in horses: A review of the literature. Veterinarni Medicina 49(11), 427–440.
- Sano Y, Matsuda K, Osaki K, Miyasho T, Tsuda T, Taniyama H. Systemic mycobacteriosis in an aborted thoroughbred fetus in Japan.. J Vet Med Sci 2014 Dec;76(12):1617-21.
- Seemann T. Snippy: Fast bacterial variant calling from NGS reads. .
- Tasler GR, Hartley WJ. Foal abortion associated with Mycobacterium terrae infection.. Vet Pathol 1981 Jan;18(1):122-5.
- Thorel MF, Huchzermeyer H, Weiss R, Fontaine JJ. Mycobacterium avium infections in animals. Literature review.. Vet Res 1997 Sep-Oct;28(5):439-47.
- Uchiya KI, Tomida S, Nakagawa T, Asahi S, Nikai T, Ogawa K. Comparative genome analyses of Mycobacterium avium reveal genomic features of its subspecies and strains that cause progression of pulmonary disease.. Sci Rep 2017 Jan 3;7:39750.
- Yano H, Iwamoto T, Nishiuchi Y, Nakajima C, Starkova DA, Mokrousov I, Narvskaya O, Yoshida S, Arikawa K, Nakanishi N, Osaki K, Nakagawa I, Ato M, Suzuki Y, Maruyama F. Population Structure and Local Adaptation of MAC Lung Disease Agent Mycobacterium avium subsp. hominissuis.. Genome Biol Evol 2017 Sep 1;9(9):2403-2417.
Citations
This article has been cited 3 times.- Li L, Maboni G, Lack A, Gomez DE. Nontuberculous Mycobacteria in Horses: A Narrative Review. Vet Sci 2023 Jul 6;10(7).
- Li L, Li S, Ma H, Akhtar MF, Tan Y, Wang T, Liu W, Khan A, Khan MZ, Wang C. An Overview of Infectious and Non-Infectious Causes of Pregnancy Losses in Equine. Animals (Basel) 2024 Jul 2;14(13).
- Morales-Vázquez MM, Meza-Serrano E, Lara-Pereyra I, Acuña-González RJ, Alonso-Morales R, Hayen-Valles S, Boeta AM, Zarco L, Lozano-Cuenca J, López-Canales JS, Flores-Herrera H. Equine Placentitis in Mares Induces the Secretion of Pro-Inflammatory Cytokine eIL-1β and the Active Extracellular Matrix Metalloproteinase (MMP)-9. Vet Sci 2023 Aug 22;10(9).
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