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Frontiers in veterinary science2015; 2; 49; doi: 10.3389/fvets.2015.00049

Tenogenically Induced Allogeneic Mesenchymal Stem Cells for the Treatment of Proximal Suspensory Ligament Desmitis in a Horse.

Abstract: Suspensory ligament injuries are a common injury in sport horses, especially in competing dressage horses. Because of the poor healing of chronic recalcitrant tendon injuries, this represents a major problem in the rehabilitation of sport horses and often compromises the return to the initial performance level. Stem cells are considered as a novel treatment for different pathologies in horses and humans. Autologous mesenchymal stem cells (MSCs) are well known for their use in the treatment of tendinopathies; however, recent studies report a safe use of allogeneic MSCs for different orthopedic applications in horses. Moreover, it has been reported that pre-differentiation of MSCs prior to injection might result in improved clinical outcomes. For all these reasons, the present case report describes the use of allogeneic tenogenically induced peripheral blood-derived MSCs for the treatment of a proximal suspensory ligament injury. During conservative management for 4 months, the horse demonstrated no improvement of a right front lameness with a Grade 2/5 on the American Association of Equine Practitioners (AAEP) scale and a clear hypo-echoic area detectable in 30% of the cross sectional area. From 4 weeks after treatment, the lameness reduced to an AAEP Grade 1/5 and a clear filling of the lesion could be noticed on ultrasound. At 12 weeks (T 4) after the first injection, a second intralesional injection with allogeneic tenogenically induced MSCs and platelet-rich plasma was given and at 4 weeks after the second injection (T 5), the horse trotted sound under all circumstances with a close to total fiber alignment. The horse went back to previous performance level at 32 weeks after the first regenerative therapy and is currently still doing so (i.e., 20 weeks later or 1 year after the first stem cell treatment). In conclusion, the present case report demonstrated a positive evolution of proximal suspensory ligament desmitis after treatment with allogeneic tenogenically induced MSCs.
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Publication Date: 2015-10-22 PubMed ID: 26664976PubMed Central: PMC4672201DOI: 10.3389/fvets.2015.00049Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research paper is about a case study of a horse treated for a suspensory ligament injury using allogenic, tenogenically induced mesenchymal stem cells. The outcome was positive, with substantial improvements noted in lameness, lesion repair, and the horse’s return to their pre-injury performance levels.

Background and Objective

  • Suspensory ligament injuries in horses, particularly in dressage horses, pose a significant challenge due to their sluggish recovery. Chronic tendon injuries hinder the rehabilitation process and usually impact the animal’s ability to return to their previous performance level.
  • Stem cells have emerged as a promising treatment for various pathologies in both horses and humans. Autologous mesenchymal stem cells (MSCs) — taken from the patient — are proven players in treating tendinopathies.
  • Recent studies have flagged the safe usage of allogeneic MSCs — stem cells from a different individual of the same species — for diverse orthopedic uses in horses.
  • Pre-differentiation of MSCs before the injection could enhance clinical outcomes, indicating that the cells are directed towards a specific tissue type before application.
  • This case study aims to document the use of allogenic tenogenically induced (prepared to develop into tendon cells) peripheral blood-derived MSCs to treat a proximal suspensory ligament injury in a horse.

Case Study Observations and Results

  • After four months of traditional management, the horse showed no signs of improvements with an obvious hypo-echoic area in 30% of the cross-sectional area and a Grade 2/5 lameness based on the American Association of Equine Practitioners (AAEP) scale.
  • Following the treatment, the lameness reduced to an AAEP Grade 1/5 in 4 weeks, and ultrasound revealed clear lesion repair.
  • Subsequently, a second injection of allogenic tenogenically induced MSCs in combination with platelet-rich plasma was administered 12 weeks after the first one.
  • Four weeks post-second injection, the horse achieved almost total fiber alignment and exhibited sound trotting in all situations.
  • Remarkably, the horse reclaimed its previous performance level 32 weeks post-first regenerative therapy and maintained it for an additional 20 weeks or a year since the first stem cell treatment.

Conclusion

  • The study showed positive developments in proximal suspensory ligament desmitis post-treatment with allogenic tenogenically induced MSCs.
  • This case study provides compelling evidence for the successful use of blood-derived, allogenic MSCs to facilitate tendon repair and healing in horses, potentially offering a promising treatment strategy for similar ligament injuries.

Cite This Article

APA
Vandenberghe A, Broeckx SY, Beerts C, Seys B, Zimmerman M, Verweire I, Suls M, Spaas JH. (2015). Tenogenically Induced Allogeneic Mesenchymal Stem Cells for the Treatment of Proximal Suspensory Ligament Desmitis in a Horse. Front Vet Sci, 2, 49. https://doi.org/10.3389/fvets.2015.00049

Publication

Frontiers in veterinary science
ISSN: 2297-1769
NlmUniqueID: 101666658
Country: Switzerland
Language: English
Volume: 2
Pages: 49

Researcher Affiliations

Vandenberghe, Aurélie
  • Global Stem Cell Technology, ANACURA Group , Evergem , Belgium ; Equine Veterinary Pratice Dr. Suls , Nederweert , Netherlands.
Broeckx, Sarah Y
  • Global Stem Cell Technology, ANACURA Group , Evergem , Belgium.
Beerts, Charlotte
  • Global Stem Cell Technology, ANACURA Group , Evergem , Belgium.
Seys, Bert
  • Equine Veterinary Pratice Dr. Suls , Nederweert , Netherlands.
Zimmerman, Marieke
  • Equine Diagnostic Center , Meldert-Lummen , Belgium.
Verweire, Ineke
  • Global Stem Cell Technology, ANACURA Group , Evergem , Belgium.
Suls, Marc
  • Equine Veterinary Pratice Dr. Suls , Nederweert , Netherlands.
Spaas, Jan H
  • Global Stem Cell Technology, ANACURA Group , Evergem , Belgium.

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