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International journal of molecular sciences2017; 18(10); doi: 10.3390/ijms18102122

The Contribution of Adipose Tissue-Derived Mesenchymal Stem Cells and Platelet-Rich Plasma to the Treatment of Chronic Equine Laminitis: A Proof of Concept.

Abstract: Laminitis, a highly debilitating disease of the foot in ungulates, is characterized by pathological changes of the complex lamellar structures that maintain the appendicular skeleton within the hoof. Laminitis is a multifactorial disease that involves perturbation of the vascular, hematological, and inflammatory homeostasis of the foot. Interestingly, the pathogenesis of the disease resembles what is observed in metabolic syndromes and sepsis-induced organ failure in humans and animals. We hypothesized that local administration of mesenchymal stem cells (MSCs) and platelet-rich plasma (PRP) might contribute to establishing an anti-inflammatory and pro-angiogenic environment, and could stimulate the injured tissue in order to restore its functional integrity. According to this assumption, an experimental protocol based on the local intravenous administration of adipose tissue-derived MSCs (aMSCs) in combination with PRP was developed for the treatment of horses affected by chronic laminitis. Nine horses with severely compromised venograms (showing grade III and IV laminitis) that had been unsuccessfully treated with conventional therapies were enrolled. aMSCs and PRP (15 × 10⁶ cells resuspended in 15 mL of PRP) were injected into the lateral or medial digital vein three times, at one-month intervals. The first administration was performed with allogeneic aMSCs, while for the following administrations, autologous aMSCs were used. There was no adverse short-term reaction to the intravenous injection of aMSCs. In the long term, venograms outlined, in all subjects, a progressive amelioration of the vascularization of the foot. An improvement in the structure and function of the hoof was also observed. No adverse events were reported during the follow-up, and the horses returned to a comfortable quality of life. Although the number of animals enrolled in the study is limited, both clinical observations and venography demonstrated an enhancement in the condition of all horses, suggesting that the regenerative therapies in chronic laminitis could be useful, and are worthy of further investigation.
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Publication Date: 2017-10-11 PubMed ID: 29019941PubMed Central: PMC5666804DOI: 10.3390/ijms18102122Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study explores how a treatment involving adipose tissue-derived mesenchymal stem cells (aMSCs) and platelet-rich plasma (PRP) can improve chronic laminitis, a serious foot disease in horses. Nine horses with advanced laminitis were treated over three months using this protocol, resulting in improved foot health and quality of life.

Deeper Understanding of the Research Paper

  • The paper presents an investigation into treating chronic laminitis, a debilitating disease affecting the feet of hoofed animals, in particular, horses. The disease is complex, involving the subversion of regular vascular, hematological, and inflammatory systems within the foot. This condition mirrors sepsis-induced organ failure and metabolic syndromes found in humans and other animals.
  • The research is based on the hypothesis that local delivery of mesenchymal stem cells (MSCs) and platelet-rich plasma (PRP) could create an anti-inflammatory and pro-angiogenic environment, potentially stimulating tissue repair and recovery. The researchers propose a treatment protocol involving intravenous administration of adipose tissue-derived MSCs (aMSCs) in conjunction with PRP for horses suffering from chronic laminitis.

Methodology and Results

  • The study involved nine horses diagnosed with severe laminitis (grade III and IV) that hadn’t responded to traditional therapies. These horses underwent a treatment involving both aMSCs and PRP injections, segmented over three one-month intervals.
  • The first injection contained allogeneic (donor) aMSCs due to the time required to grow autologous (patient’s own) aMSCs, and the following injections were comprised of autologous aMSCs. All injections contained 15×10⁶ cells resuspended in 15ml of PRP.
  • The horses showed no short-term adverse reactions to the aMSCs injections. Long-term observation revealed a positive progression in foot vascularization and overall hoof structure/function. During the study and subsequent follow-up, the horses appeared to return to a high quality of life with no negative side effects reported.

Conclusion

  • Although there is a cautionary note that the sample size of the study was small, the alleviation of the condition in all the horses included strongly suggests that this treatment protocol shows promise. The researchers call for continued investigation into the benefits of such regenerative therapies in managing chronic laminitis in horses.

Cite This Article

APA
Angelone M, Conti V, Biacca C, Battaglia B, Pecorari L, Piana F, Gnudi G, Leonardi F, Ramoni R, Basini G, Dotti S, Renzi S, Ferrari M, Grolli S. (2017). The Contribution of Adipose Tissue-Derived Mesenchymal Stem Cells and Platelet-Rich Plasma to the Treatment of Chronic Equine Laminitis: A Proof of Concept. Int J Mol Sci, 18(10). https://doi.org/10.3390/ijms18102122

Publication

International journal of molecular sciences
ISSN: 1422-0067
NlmUniqueID: 101092791
Country: Switzerland
Language: English
Volume: 18
Issue: 10

Researcher Affiliations

Angelone, Mario
  • Dipartimento Scienze Mediche Veterinarie, Università di Parma, Via del taglio, 10, 43126 Parma, Italy. marioangelonevet@libero.it.
Conti, Virna
  • Dipartimento Scienze Mediche Veterinarie, Università di Parma, Via del taglio, 10, 43126 Parma, Italy. virna.conti@unipr.it.
Biacca, Cristiano
  • Veterinary Practitioner, Dipartimento Scienze Mediche Veterinarie, Università di Parma, Via del taglio, 10, 43126 Parma, Italy. cristiano.biacca@libero.it.
Battaglia, Beatrice
  • Veterinary Practitioner, Dipartimento Scienze Mediche Veterinarie, Università di Parma, Via del taglio, 10, 43126 Parma, Italy. beatricechiarabattaglia@gmail.com.it.
Pecorari, Laura
  • Veterinary Practitioner, Dipartimento Scienze Mediche Veterinarie, Università di Parma, Via del taglio, 10, 43126 Parma, Italy. laurapecorari.dvm@gmail.com.
Piana, Francesco
  • Veterinary Practitioner, Dipartimento Scienze Mediche Veterinarie, Università di Parma, Via del taglio, 10, 43126 Parma, Italy. f.piana.vet@gmail.com.
Gnudi, Giacomo
  • Dipartimento Scienze Mediche Veterinarie, Università di Parma, Via del taglio, 10, 43126 Parma, Italy. giacomo.gnudi@unipr.it.
Leonardi, Fabio
  • Dipartimento Scienze Mediche Veterinarie, Università di Parma, Via del taglio, 10, 43126 Parma, Italy. fabio.leonardi@unipr.it.
Ramoni, Roberto
  • Dipartimento Scienze Mediche Veterinarie, Università di Parma, Via del taglio, 10, 43126 Parma, Italy. roberto.ramoni@unipr.it.
Basini, Giuseppina
  • Dipartimento Scienze Mediche Veterinarie, Università di Parma, Via del taglio, 10, 43126 Parma, Italy. giuseppina.basini@unipr.it.
Dotti, Silvia
  • Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna, Via Bianchi 9, 25124 Brescia, Italy. silvia.dotti@izsler.it.
Renzi, Sabrina
  • Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna, Via Bianchi 9, 25124 Brescia, Italy. sabrina.renzi@izsler.it.
Ferrari, Maura
  • Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna, Via Bianchi 9, 25124 Brescia, Italy. maura1.ferrari@libero.it.
Grolli, Stefano
  • Dipartimento Scienze Mediche Veterinarie, Università di Parma, Via del taglio, 10, 43126 Parma, Italy. stefano.grolli@unipr.it.

MeSH Terms

  • Adipose Tissue / cytology
  • Administration, Intravenous
  • Animals
  • Chronic Disease
  • Foot Diseases / therapy
  • Foot Diseases / veterinary
  • Hoof and Claw / pathology
  • Horse Diseases / therapy
  • Horses
  • Inflammation / therapy
  • Inflammation / veterinary
  • Mesenchymal Stem Cell Transplantation
  • Mesenchymal Stem Cells / cytology
  • Platelet-Rich Plasma
  • Quality of Life
  • Regenerative Medicine

Conflict of Interest Statement

The authors declare no conflicts of interest.

References

This article includes 50 references
  1. Katz L.M., Bailey S.R.. A review of recent advances and current hypotheses on the pathogenesis of acute laminitis.. Equine Vet. J. 2012;44:752–761.
    doi: 10.1111/j.2042-3306.2012.00664.xpubmed: 23106629google scholar: lookup
  2. Pollitt C.C.. The anatomy and physiology of the suspensory apparatus of the distal phalanx.. Vet. Clin. North Am. Equine Pract. 2010;26:29–49.
    doi: 10.1016/j.cveq.2010.01.005pubmed: 20381734google scholar: lookup
  3. Engiles J.B., Galantino-Homer H.L., Boston R., McDonald D., Dishowitz M., Hankenson K.D.. Osteopathology in the equine distal phalanx associated with the development and progression of laminitis.. Vet. Pathol. 2015;52:928–944.
    doi: 10.1177/0300985815588604pubmed: 26063172google scholar: lookup
  4. Eades S.C.. Overview of current laminitis research.. Vet. Clin. North Am. Equine Pract. 2010;26:51–63.
    doi: 10.1016/j.cveq.2010.01.001pubmed: 20381735google scholar: lookup
  5. Morgan R., Keen J., McGowan C.. Equine metabolic syndrome.. Vet. Rec. 2015;177:173–179.
    doi: 10.1136/vr.103226pmc: PMC4552932pubmed: 26273009google scholar: lookup
  6. Eustace R.A.. Clinical presentation, diagnosis, and prognosis of chronic laminitis in Europe.. Vet. Clin. North Am. Equine Pract. 2010;26:391–405.
    doi: 10.1016/j.cveq.2010.06.005pubmed: 20699183google scholar: lookup
  7. Pollitt C., Collins S.N.. Chronic laminitis.. In: Ross M.W., Dyson S.J., editors. Diagnosis and Management of Lameness in the Horse. 2nd ed. Elsevier Saunders Health Sciences; St. Louis, MO, USA: 2010. pp. 374–377.
  8. Bailey S.R., Marr C.M., Elliott J.. Current research and theories on the pathogenesis of acute laminitis in the horse.. Vet. J. 2004;167:129–142.
    doi: 10.1016/S1090-0233(03)00120-5pubmed: 14975387google scholar: lookup
  9. Pollitt C.. Pathophysiology of laminitis.. In: Ross M.W., Dyson S.J., editors. Diagnosis and Management of Lameness in the Horse. 2nd ed. Elsevier Saunders Health Sciences; St. Louis, MO, USA: 2010. pp. 366–371.
  10. Black S.J.. Extracellular matrix, leukocyte migration and laminitis.. Vet. Immunol. Immunopathol. 2009;129:161–163.
    doi: 10.1016/j.vetimm.2008.11.010pubmed: 19110317google scholar: lookup
  11. Steelman S.M., Johnson D., Wagner B., Stokes A., Chowdhary B.P.. Cellular and humoral immunity in chronic equine laminitis.. Vet. Immunol. Immunopathol. 2013;153:217–226.
    doi: 10.1016/j.vetimm.2013.03.001pubmed: 23521925google scholar: lookup
  12. Belknap J.K., Faleiros R., Black S.J., Johnson P.J., Eades S.. The laminar leukocyte: From sepsis to endocrinopathic models of laminitis.. J. Vet. Sci. 2011;31:584–585.
    doi: 10.1016/j.jevs.2011.09.034google scholar: lookup
  13. Loftus J.P., Johnson P.J., Belknap J.K., Pettigrew A., Black S.J.. Leukocyte-derived and endogenous matrix metalloproteinases in the lamellae of horses with naturally acquired and experimentally induced laminitis.. Vet. Immunol. Immunopathol. 2009;129:221–230.
    doi: 10.1016/j.vetimm.2008.11.003pubmed: 19101039google scholar: lookup
  14. Stewart A.J., Pettigrew A., Cochran A.M., Belknap J.K.. Indices of inflammation in the lung and liver in the early stages of the black walnut extract model of equine laminitis.. Vet. Immunol. Immunopathol. 2009;129:254–260.
    doi: 10.1016/j.vetimm.2008.11.001pubmed: 19124160google scholar: lookup
  15. Tadros E.M., Frank N., Newkirk K.M., Donnell R.L., Horohov D.W.. Effects of a “two-hit” model of organ damage on the systemic inflammatory response and development of laminitis in horses.. Vet. Immunol. Immunopathol. 2012;150:90–100.
    doi: 10.1016/j.vetimm.2012.09.002pubmed: 23026157google scholar: lookup
  16. Belknap J.K., Moore J.N., Crouser E.C.. Sepsis-From human organ failure to laminar failure.. Vet. Immunol. Immunopathol. 2009;129:155–157.
    doi: 10.1016/j.vetimm.2008.11.013pubmed: 19131119google scholar: lookup
  17. Geor R., Frank N.. Metabolic syndrome-From human organ disease to laminar failure in equids.. Vet. Immunol. Immunopathol. 2009;129:151–154.
    doi: 10.1016/j.vetimm.2008.11.012pubmed: 19110319google scholar: lookup
  18. Monsel A., Zhu Y.G., Gennai S., Hao Q., Liu J., Lee J.W.. Cell-based therapy for acute organ injury: Preclinical evidence and ongoing clinical trials using mesenchymal stem cells.. Anesthesiology 2014;121:1099–1121.
    doi: 10.1097/ALN.0000000000000446pmc: PMC4206665pubmed: 25211170google scholar: lookup
  19. Matthay M.A., Pati S., Lee J.W.. Concise Review: Mesenchymal Stem (Stromal) Cells: Biology and Preclinical Evidence for Therapeutic Potential for Organ Dysfunction Following Trauma or Sepsis.. Stem Cells 2017;35:316–324.
    doi: 10.1002/stem.2551pubmed: 27888550google scholar: lookup
  20. Prockop D.J., Oh J.Y.. Mesenchymal stem/stromal cells (MSCs): Role as guardians of inflammation.. Mol. Ther. 2012;20:14–20.
    doi: 10.1038/mt.2011.211pmc: PMC3255583pubmed: 22008910google scholar: lookup
  21. Inan M., Bakar E., Cerkezkayabekir A., Sanal F., Ulucam E., Subaşı C., Karaöz E.. Mesenchymal stem cells increase antioxidant capacity in intestinal ischemia/reperfusion damage.. J. Pediatr. Surg. 2017;52:1196–1206.
    doi: 10.1016/j.jpedsurg.2016.12.024pubmed: 28118930google scholar: lookup
  22. Gu W., Hong X., Potter C., Qu A., Xu Q.. Mesenchymal stem cells and vascular regeneration.. Microcirculation 2017.
    doi: 10.1111/micc.12324pubmed: 27681821google scholar: lookup
  23. Tobita M., Tajima S., Mizuno H.. Adipose tissue-derived mesenchymal stem cells and platelet-rich plasma: Stem cell transplantation methods that enhance stemness.. Stem Cell Res. Ther. 2015;6:215.
    doi: 10.1186/s13287-015-0217-8pmc: PMC4635588pubmed: 26541973google scholar: lookup
  24. Iacono E., Brunori L., Pirrone A., Pagliaro P.P., Ricci F., Tazzari P.L., Merlo B.. Isolation, characterization and differentiation of mesenchymal stem cells from amniotic fluid, umbilical cord blood and Wharton’s jelly in the horse.. Reproduction 2012;143:455–468.
    doi: 10.1530/REP-10-0408pubmed: 22274885google scholar: lookup
  25. Ranera B., Lyahyai J., Romero A., Vázquez F.J., Remacha A.R., Bernal M.L., Zaragoza P., Rodellar C., Martín-Burriel I.. Immunophenotype and gene expression profiles of cell surface markers of mesenchymal stem cells derived from equine bone marrow and adipose tissue.. Vet. Immunol. Immunopathol. 2011;144:147–154.
    doi: 10.1016/j.vetimm.2011.06.033pubmed: 21782255google scholar: lookup
  26. Zhao Y., Zhang H.. Update on the mechanisms of homing of adipose tissue-derived stem cells.. Cytotherapy 2016;18:816–827.
    doi: 10.1016/j.jcyt.2016.04.008pubmed: 27260205google scholar: lookup
  27. Block G.J., Ohkouchi S., Fung F., Frenkel J., Gregory C., Pochampally R., DiMattia G., Sullivan D.E., Prockop D.J.. Multipotent stromal cells (MSCs) are activated to reduce apoptosis in part by upregulation and secretion of stanniocalcin-1 (STC-1). Stem Cells 2009;27:670–681.
    doi: 10.1002/stem.20080742pmc: PMC4742302pubmed: 19267325google scholar: lookup
  28. Ono M., Ohkouchi S., Kanehira M., Tode N., Kobayashi M., Ebina M., Nukiwa T., Irokawa T., Ogawa H., Akaike T.. Mesenchymal stem cells correct inappropriate epithelial-mesenchyme relation in pulmonary fibrosis using stanniocalcin-1.. Mol. Ther. 2015;23:549–560.
    doi: 10.1038/mt.2014.217pmc: PMC4351453pubmed: 25373521google scholar: lookup
  29. Lee K.C., Lin H.C., Huang Y.H., Hung S.C.. Allo-transplantation of mesenchymal stem cells attenuates hepatic injury through IL1Ra dependent macrophage switch in a mouse model of liver disease.. J. Hepatol. 2015;63:1405–1412.
    doi: 10.1016/j.jhep.2015.07.035pubmed: 26276675google scholar: lookup
  30. Lee R.H., Yu J.M., Foskett A.M., Peltier G., Reneau J.C., Bazhanov N., Oh J.Y., Prockop D.J.. TSG-6 as a biomarker to predict efficacy of human mesenchymal stem/progenitor cells (hMSCs) in modulating sterile inflammation in vivo.. Proc. Natl. Acad. Sci. USA. 2014;111:16766–16771.
    doi: 10.1073/pnas.1416121111pmc: PMC4250139pubmed: 25385603google scholar: lookup
  31. Luo Q., Zhang B., Kuang D., Song G.. Role of Stromal-Derived Factor-1 in Mesenchymal Stem Cell Paracrine-Mediated Tissue Repair.. Curr. Stem Cell Res. Ther. 2016;11:585–592.
    doi: 10.2174/1574888X11666160614102629pubmed: 27306402google scholar: lookup
  32. Hocking A.M.. The Role of Chemokines in Mesenchymal Stem Cell Homing to Wounds.. Adv. Wound Care. 2015;4:623–630.
    doi: 10.1089/wound.2014.0579pmc: PMC4620518pubmed: 26543676google scholar: lookup
  33. Zhou P., Liu Z., Li X., Zhang B., Wang X., Lan J., Shi Q., Li D., Ju X.. Migration ability and Toll-like receptor expression of human mesenchymal stem cells improves significantly after three-dimensional culture.. Biochem. Biophys. Res. Commun. 2017;491:323–328.
    doi: 10.1016/j.bbrc.2017.07.102pubmed: 28734835google scholar: lookup
  34. Pascucci L., Alessandri G., Dall’Aglio C., Mercati F., Coliolo P., Bazzucchi C., Dante S., Petrini S., Curina G., Ceccarelli P.. Membrane vesicles mediate pro-angiogenic activity of equine adipose-derived mesenchymal stromal cells.. Vet. J. 2014;202:361–366.
    doi: 10.1016/j.tvjl.2014.08.021pubmed: 25241947google scholar: lookup
  35. Gentile P., Scioli M.G., Bielli A., Orlandi A., Cervelli V.. Concise Review: The Use of Adipose-Derived Stromal Vascular Fraction Cells and Platelet Rich Plasma in Regenerative Plastic Surgery.. Stem Cells 2017;35:117–134.
    doi: 10.1002/stem.2498pubmed: 27641055google scholar: lookup
  36. Riccò S., Renzi S., Del Bue M., Conti V., Merli E., Ramoni R., Lucarelli E., Gnudi G., Ferrari M., Grolli S.. Allogeneic adipose tissue-derived mesenchymal stem cells in combination with platelet rich plasma are safe and effective in the therapy of superficial digital flexor tendonitis in the horse.. Int. J. Immunopathol. Pharmacol. 2013;26:61–68.
    doi: 10.1177/03946320130260S108pubmed: 24046950google scholar: lookup
  37. Broeckx S., Zimmerman M., Crocetti S., Suls M., Mariën T., Ferguson S.J., Chiers K., Duchateau L., Franco-Obregón A., Wuertz K.. Regenerative therapies for equine degenerative joint disease: A preliminary study.. PLoS ONE 2014;9:e85917.
    doi: 10.1371/journal.pone.0085917pmc: PMC3896436pubmed: 24465787google scholar: lookup
  38. Canapp S.O., Canapp D.A., Ibrahim V., Carr B.J., Cox C., Barrett J.G.. The Use of Adipose-Derived Progenitor Cells and Platelet-Rich Plasma Combination for the Treatment of Supraspinatus Tendinopathy in 55 Dogs: A Retrospective Study.. Front. Vet. Sci. 2016;3:61.
    doi: 10.3389/fvets.2016.00061pmc: PMC5016533pubmed: 27668218google scholar: lookup
  39. Renzi S., Riccò S., Dotti S., Sesso L., Grolli S., Cornali M., Carlin S., Patruno M., Cinotti S., Ferrari M.. Autologous bone marrow mesenchymal stromal cells for regeneration of injured equine ligaments and tendons: A clinical report.. Res. Vet. Sci. 2013;95:272–277.
    doi: 10.1016/j.rvsc.2013.01.017pubmed: 23419936google scholar: lookup
  40. Carrade D.D., Borjesson D.L.. Immunomodulation by mesenchymal stem cells in veterinary species.. Comp. Med. 2013;63:207–217.
    pmc: PMC3690426pubmed: 23759523
  41. Kol A., Wood J.A., Carrade Holt D.D., Gillette J.A., Bohannon-Worsley L.K., Puchalski S.M., Walker N.J., Clark K.C., Watson J.L., Borjesson D.L.. Multiple intravenous injections of allogeneic equine mesenchymal stem cells do not induce a systemic inflammatory response but do alter lymphocyte subsets in healthy horses.. Stem Cell Res. Ther. 2015;6:73.
    doi: 10.1186/s13287-015-0050-0pmc: PMC4446064pubmed: 25888916google scholar: lookup
  42. Ardanaz N., Vázquez F.J., Romero A., Remacha A.R., Barrachina L., Sanz A., Ranera B., Vitoria A., Albareda J., Prades M.. Inflammatory response to the administration of mesenchymal stem cells in an equine experimental model: Effect of autologous, and single and repeat doses of pooled allogeneic cells in healthy joints.. BMC Vet. Res. 2016;12:65.
    doi: 10.1186/s12917-016-0692-xpmc: PMC4815220pubmed: 27029614google scholar: lookup
  43. Owens S.D., Kol A., Walker N.J., Borjesson D.L.. Allogeneic mesenchymal stem cell treatment induces specific alloantibodies in horses.. Stem Cells Int. 2016;2016:5830103.
    doi: 10.1155/2016/5830103pmc: PMC5018342pubmed: 27648075google scholar: lookup
  44. Pezzanite L.M., Fortier L.A., Antczak D.F., Cassano J.M., Brosnahan M.M., Miller D., Schnabel L.V.. Equine allogeneic bone marrow-derived mesenchymal stromal cells elicit antibody responses in vivo.. Stem Cell Res. Ther. 2015;6:54.
    doi: 10.1186/s13287-015-0053-xpmc: PMC4414005pubmed: 25889095google scholar: lookup
  45. Sole A., Spriet M., Galuppo L.D., Padgett K.A., Borjesson D.L., Wisner E.R., Vidal M.A.. Scintigraphic evaluation of intra-arterial and intravenous regional limb perfusion of allogeneic bone marrow-derived mesenchymal stem cells in the normal equine distal limb using (99m) Tc-HMPAO.. Equine Vet. J. 2012;44:594–599.
    doi: 10.1111/j.2042-3306.2011.00530.xpubmed: 22212017google scholar: lookup
  46. Baldwin G.I., Pollitt C.C.. Progression of venographic changes after experimentally induced laminitis.. Vet. Clin. North Am. Equine Pract. 2010;26:135–140.
    doi: 10.1016/j.cveq.2009.12.005pubmed: 20381742google scholar: lookup
  47. De Mattos Carvalho A., Alves A.L., Golim M.A., Moroz A., Hussni C.A., de Oliveira P.G., Deffune E.. Isolation and immunophenotypic characterization of mesenchymal stem cells derived from equine species adipose tissue.. Vet. Immunol. Immunopathol. 2009;132:303–306.
    doi: 10.1016/j.vetimm.2009.06.014pubmed: 19647331google scholar: lookup
  48. Barberini D.J., Freitas N.P., Magnoni M.S., Maia L., Listoni A.J., Heckler M.C., Sudano M.J., Golim M.A., da Cruz Landim-Alvarenga F., Amorim R.M.. Equine mesenchymal stem cells from bone marrow, adipose tissue and umbilical cord: Immunophenotypic characterization and differentiation potential.. Stem Cell Res. Ther. 2014;5:25.
    doi: 10.1186/scrt414pmc: PMC4055040pubmed: 24559797google scholar: lookup
  49. Floyd A.E.. Grading the laminitic horse.. In: Floyd A.E., Mansmann R.A., editors. Equine Podiatry. Elsevier Saunders; Philadelphia, PA, USA: 2007. pp. 320–327.
  50. D’Arpe L., Bernardini D.. Digital venography in horses and its clinical application in Europe.. Vet. Clin. North Am. Equine Pract. 2010;26:339–359.
    doi: 10.1016/j.cveq.2010.06.006pubmed: 20699179google scholar: lookup

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  2. Narasimha RB, Shreya S, Jayabal VA, Yadav V, Rath PK, Mishra BP, Kancharla S, Kolli P, Mandadapu G, Kumar S, Mohanty AK, Jena MK. Stem Cell Therapy for Diseases of Livestock Animals: An In-Depth Review. Vet Sci 2025 Jan 17;12(1).
    doi: 10.3390/vetsci12010067pubmed: 39852942google scholar: lookup
  3. Bernardini C, Romagnoli N, Casalini I, Turba ME, Spadari A, Forni M, Gentilini F. Freeze-drying protocols and methods of maintaining the in-vitro biological activity of horse platelet lysate. Int J Vet Sci Med 2024;12(1):71-80.
    doi: 10.1080/23144599.2024.2380586pubmed: 39119550google scholar: lookup
  4. Andreoli V, Berni P, Conti V, Ramoni R, Basini G, Grolli S. Mesenchymal Stromal Cells Derived from Canine Adipose Tissue: Evaluation of the Effect of Different Shipping Vehicles Used for Clinical Administration. Int J Mol Sci 2024 Mar 18;25(6).
    doi: 10.3390/ijms25063426pubmed: 38542399google scholar: lookup
  5. Tamura N, Heidari N, Faragher RGA, Smith RKW, Dudhia J. Effects of resveratrol and its analogues on the cell cycle of equine mesenchymal stem/stromal cells. J Equine Sci 2023 Sep;34(3):67-72.
    doi: 10.1294/jes.34.67pubmed: 37781569google scholar: lookup
  6. Pielok A, Kępska M, Steczkiewicz Z, Grobosz S, Bourebaba L, Marycz K. Equine Hoof Progenitor Cells Display Increased Mitochondrial Metabolism and Adaptive Potential to a Highly Pro-Inflammatory Microenvironment. Int J Mol Sci 2023 Jul 14;24(14).
    doi: 10.3390/ijms241411446pubmed: 37511204google scholar: lookup
  7. Lu T, Zheng C, Fan Z. Cardamonin suppressed the migration, invasion, epithelial mesenchymal transition (EMT) and lung metastasis of colorectal cancer cells by down-regulating ADRB2 expression. Pharm Biol 2022 Dec;60(1):1011-1021.
    doi: 10.1080/13880209.2022.2069823pubmed: 35645356google scholar: lookup
  8. Miguel-Pastor L, Satué K, Chicharro D, Torres-Torrillas M, Del Romero A, Peláez P, Carrillo JM, Cuervo B, Sopena JJ, Cerón JJ, Rubio M. Evaluation of a Standardized Protocol for Plasma Rich in Growth Factors Obtention in Cats: A Prospective Study. Front Vet Sci 2022;9:866547.
    doi: 10.3389/fvets.2022.866547pubmed: 35498746google scholar: lookup
  9. Uberti B, Plaza A, Henríquez C. Pre-conditioning Strategies for Mesenchymal Stromal/Stem Cells in Inflammatory Conditions of Livestock Species. Front Vet Sci 2022;9:806069.
    doi: 10.3389/fvets.2022.806069pubmed: 35372550google scholar: lookup
  10. Berni P, Leonardi F, Conti V, Ramoni R, Grolli S, Mattioli G. Case Report: A Novel Ventilated Thermoplastic Mesh Bandage for Post-operative Management of Large Soft Tissue Defects: A Case Series of Three Dogs Treated With Autologous Platelet Concentrates. Front Vet Sci 2021;8:704567.
    doi: 10.3389/fvets.2021.704567pubmed: 34540933google scholar: lookup
  11. Seidel SRT, de Souza AF, Fülber J, Bogossian PM, Rodrigues NNP, Baccarin RYA. Evaluation of platelet-rich plasma applied in the coronary band of healthy equine hooves. Can Vet J 2021 Jul;62(7):729-735.
    pubmed: 34219782
  12. Cequier A, Sanz C, Rodellar C, Barrachina L. The Usefulness of Mesenchymal Stem Cells beyond the Musculoskeletal System in Horses. Animals (Basel) 2021 Mar 25;11(4).
    doi: 10.3390/ani11040931pubmed: 33805967google scholar: lookup
  13. Ribitsch I, Oreff GL, Jenner F. Regenerative Medicine for Equine Musculoskeletal Diseases. Animals (Basel) 2021 Jan 19;11(1).
    doi: 10.3390/ani11010234pubmed: 33477808google scholar: lookup
  14. Chang Y, Yang M, Ke S, Zhang Y, Xu G, Li Z. Effect of Platelet-Rich Plasma on Intervertebral Disc Degeneration In Vivo and In Vitro: A Critical Review. Oxid Med Cell Longev 2020;2020:8893819.
    doi: 10.1155/2020/8893819pubmed: 33299533google scholar: lookup
  15. Suelzu CM, Conti V, Khalidy Y, Montagna S, Strusi G, Di Lecce R, Berni P, Basini G, Ramoni R, Grolli S. Xenobiotic-Free Medium Guarantees Expansion of Adipose Tissue-Derived Canine Mesenchymal Stem Cells Both in 3D Fibrin-Based Matrices and in 2D Plastic Surface Cultures. Cells 2020 Dec 2;9(12).
    doi: 10.3390/cells9122578pubmed: 33276432google scholar: lookup
  16. Bukowska J, Szóstek-Mioduchowska AZ, Kopcewicz M, Walendzik K, Machcińska S, Gawrońska-Kozak B. Adipose-Derived Stromal/Stem Cells from Large Animal Models: from Basic to Applied Science. Stem Cell Rev Rep 2021 Jun;17(3):719-738.
    doi: 10.1007/s12015-020-10049-ypubmed: 33025392google scholar: lookup
  17. Gugjoo MB, Hussain S, Amarpal, Shah RA, Dhama K. Mesenchymal Stem Cell-Mediated Immuno-Modulatory and Anti- Inflammatory Mechanisms in Immune and Allergic Disorders. Recent Pat Inflamm Allergy Drug Discov 2020;14(1):3-14.
    doi: 10.2174/1872213X14666200130100236pubmed: 32000656google scholar: lookup
  18. Kardos D, Simon M, Vácz G, Hinsenkamp A, Holczer T, Cseh D, Sárközi A, Szenthe K, Bánáti F, Szathmary S, Nehrer S, Kuten O, Masteling M, Lacza Z, Hornyák I. The Composition of Hyperacute Serum and Platelet-Rich Plasma Is Markedly Different despite the Similar Production Method. Int J Mol Sci 2019 Feb 8;20(3).
    doi: 10.3390/ijms20030721pubmed: 30743992google scholar: lookup
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