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Home / Equine Research Bank / PLoS One / The Effect of Different Types of Musculoskeletal Injuries on...
PloS one2015; 10(10); e0140673; doi: 10.1371/journal.pone.0140673

The Effect of Different Types of Musculoskeletal Injuries on Blood Concentration of Serum Amyloid A in Thoroughbred Racehorses.

Abstract: Training-induced muscle, skeletal and joint trauma may result in acute phase response reflected by the changes in the blood concentration of serum amyloid A (SAA) in racehorses. It remains yet unclear if such systemic reaction could be triggered by sport injuries and what is the impact of different types of musculoskeletal trauma on SAA concentrations in racehorses. This study aimed to determine changes in the SAA blood concentration in racehorses with different types of injuries of musculoskeletal system. Methods: The study involved 28 racehorses diagnosed after the race with bone fractures (n = 7), dorsal metacarpal disease (n = 11), joint trauma (n = 4) or tendon and muscle trauma (n = 6) and 28 healthy control racehorses. Serum samples were collected twice, between 1 and 4 days of the injury or succesful completion of the race. SAA concentration was measured using the commercial ELISA kit. Differences between mean SAA concentration in respective groups were analyzed using ANOVA and Tukey post-hoc test. Results: Mean SAA concentration within the first 4 days of the injury of muscle and tendon was significantly higher than in bone fractures, dorsal metacarpal disease, joint trauma or in the healthy horses (p<0,001). There were no significant differences between the other groups. Conclusions: Strain injuries of muscle and tendons can cause a moderate increase in SAA blood concentration in racehorses, reflecting the occurrence of the acute phase response. Similar reaction is not observed in the stress-related bone injuries.
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Publication Date: 2015-10-14 PubMed ID: 26466121PubMed Central: PMC4605491DOI: 10.1371/journal.pone.0140673Google Scholar: Lookup
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Summary

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The research article presents a study on the impact of different types of musculoskeletal injuries on the concentration of serum amyloid A (SAA) in the blood of thoroughbred racehorses. The findings suggest that strain injuries of muscles and tendons can cause a moderate increase in SAA blood concentration.

Objective and Methods of the Study

  • The objective of the study was to investigate how different types of musculoskeletal injuries in racehorses influence the concentration of SAA in the blood.
  • The study involved 28 racehorses diagnosed after the race with different types of injuries such as bone fractures, dorsal metacarpal disease, joint trauma or tendon and muscle trauma, along with 28 healthy control racehorses.
  • Serum samples from these horses were collected twice, between 1 and 4 days of the injury or after the successful completion of the race.
  • The SAA concentration was measured using a commercial ELISA kit, a common tool in molecular biology that uses antibodies to detect the presence of specific proteins like SAA in blood samples.
  • The researchers then compared the mean SAA concentration in the different horse groups using a statistical method called ANOVA (Analysis of Variance), followed by the Tukey post-hoc test to find out where the differences lay.

Key Findings

  • The study discovered that the mean SAA concentration within the first 4 days of muscle and tendon injury was significantly higher than in horses with bone fractures, dorsal metacarpal disease, joint trauma or in the healthy horses.
  • There were no significant differences between the other groups in terms of SAA concentration.

Conclusions

  • The results suggest that strain injuries of muscles and tendons can result in a moderate increase in SAA blood concentration in racehorses, reflecting an acute phase response.
  • In contrast, this type of reaction is not observed in stress-related bone injuries.

Cite This Article

APA
Turło A, Cywińska A, Czopowicz M, Witkowski L, Niedźwiedź A, Słowikowska M, Borowicz H, Jaśkiewicz A, Winnicka A. (2015). The Effect of Different Types of Musculoskeletal Injuries on Blood Concentration of Serum Amyloid A in Thoroughbred Racehorses. PLoS One, 10(10), e0140673. https://doi.org/10.1371/journal.pone.0140673

Publication

PloS one
ISSN: 1932-6203
NlmUniqueID: 101285081
Country: United States
Language: English
Volume: 10
Issue: 10
Pages: e0140673

Researcher Affiliations

Turło, Agnieszka
  • Department of Pathology and Veterinary Diagnostics, Faculty of Veterinary Medicine, Warsaw University of Life Sciences-SGGW, Warsaw, Poland.
Cywińska, Anna
  • Department of Pathology and Veterinary Diagnostics, Faculty of Veterinary Medicine, Warsaw University of Life Sciences-SGGW, Warsaw, Poland.
Czopowicz, Michał
  • Laboratory of Veterinary Epidemiology and Economics, Faculty of Veterinary Medicine, Warsaw University of Life Sciences-SGGW, Warsaw, Poland.
Witkowski, Lucjan
  • Laboratory of Veterinary Epidemiology and Economics, Faculty of Veterinary Medicine, Warsaw University of Life Sciences-SGGW, Warsaw, Poland.
Niedźwiedź, Artur
  • Department of Internal Diseases with Clinic for Horses, Dogs and Cats, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life Sciences, Wrocław, Poland.
Słowikowska, Malwina
  • Department of Internal Diseases with Clinic for Horses, Dogs and Cats, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life Sciences, Wrocław, Poland.
Borowicz, Hieronim
  • Department of Internal Diseases with Clinic for Horses, Dogs and Cats, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life Sciences, Wrocław, Poland.
Jaśkiewicz, Anna
  • Department of Pathology and Veterinary Diagnostics, Faculty of Veterinary Medicine, Warsaw University of Life Sciences-SGGW, Warsaw, Poland.
Winnicka, Anna
  • Department of Pathology and Veterinary Diagnostics, Faculty of Veterinary Medicine, Warsaw University of Life Sciences-SGGW, Warsaw, Poland.

MeSH Terms

  • Animals
  • Case-Control Studies
  • Horse Diseases / blood
  • Horses
  • Musculoskeletal Diseases / veterinary
  • Serum Amyloid A Protein
  • Wounds and Injuries / veterinary

Conflict of Interest Statement

Competing Interests: The authors have declared that no competing interests exist.

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This article has been cited 7 times.
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