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PloS one2015; 10(10); e0140673; doi: 10.1371/journal.pone.0140673

The Effect of Different Types of Musculoskeletal Injuries on Blood Concentration of Serum Amyloid A in Thoroughbred Racehorses.

Abstract: Training-induced muscle, skeletal and joint trauma may result in acute phase response reflected by the changes in the blood concentration of serum amyloid A (SAA) in racehorses. It remains yet unclear if such systemic reaction could be triggered by sport injuries and what is the impact of different types of musculoskeletal trauma on SAA concentrations in racehorses. This study aimed to determine changes in the SAA blood concentration in racehorses with different types of injuries of musculoskeletal system. Methods: The study involved 28 racehorses diagnosed after the race with bone fractures (n = 7), dorsal metacarpal disease (n = 11), joint trauma (n = 4) or tendon and muscle trauma (n = 6) and 28 healthy control racehorses. Serum samples were collected twice, between 1 and 4 days of the injury or succesful completion of the race. SAA concentration was measured using the commercial ELISA kit. Differences between mean SAA concentration in respective groups were analyzed using ANOVA and Tukey post-hoc test. Results: Mean SAA concentration within the first 4 days of the injury of muscle and tendon was significantly higher than in bone fractures, dorsal metacarpal disease, joint trauma or in the healthy horses (p<0,001). There were no significant differences between the other groups. Conclusions: Strain injuries of muscle and tendons can cause a moderate increase in SAA blood concentration in racehorses, reflecting the occurrence of the acute phase response. Similar reaction is not observed in the stress-related bone injuries.
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Publication Date: 2015-10-14 PubMed ID: 26466121PubMed Central: PMC4605491DOI: 10.1371/journal.pone.0140673Google Scholar: Lookup
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Summary

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The research article presents a study on the impact of different types of musculoskeletal injuries on the concentration of serum amyloid A (SAA) in the blood of thoroughbred racehorses. The findings suggest that strain injuries of muscles and tendons can cause a moderate increase in SAA blood concentration.

Objective and Methods of the Study

  • The objective of the study was to investigate how different types of musculoskeletal injuries in racehorses influence the concentration of SAA in the blood.
  • The study involved 28 racehorses diagnosed after the race with different types of injuries such as bone fractures, dorsal metacarpal disease, joint trauma or tendon and muscle trauma, along with 28 healthy control racehorses.
  • Serum samples from these horses were collected twice, between 1 and 4 days of the injury or after the successful completion of the race.
  • The SAA concentration was measured using a commercial ELISA kit, a common tool in molecular biology that uses antibodies to detect the presence of specific proteins like SAA in blood samples.
  • The researchers then compared the mean SAA concentration in the different horse groups using a statistical method called ANOVA (Analysis of Variance), followed by the Tukey post-hoc test to find out where the differences lay.

Key Findings

  • The study discovered that the mean SAA concentration within the first 4 days of muscle and tendon injury was significantly higher than in horses with bone fractures, dorsal metacarpal disease, joint trauma or in the healthy horses.
  • There were no significant differences between the other groups in terms of SAA concentration.

Conclusions

  • The results suggest that strain injuries of muscles and tendons can result in a moderate increase in SAA blood concentration in racehorses, reflecting an acute phase response.
  • In contrast, this type of reaction is not observed in stress-related bone injuries.

Cite This Article

APA
Turło A, Cywińska A, Czopowicz M, Witkowski L, Niedźwiedź A, Słowikowska M, Borowicz H, Jaśkiewicz A, Winnicka A. (2015). The Effect of Different Types of Musculoskeletal Injuries on Blood Concentration of Serum Amyloid A in Thoroughbred Racehorses. PLoS One, 10(10), e0140673. https://doi.org/10.1371/journal.pone.0140673

Publication

PloS one
ISSN: 1932-6203
NlmUniqueID: 101285081
Country: United States
Language: English
Volume: 10
Issue: 10
Pages: e0140673

Researcher Affiliations

Turło, Agnieszka
  • Department of Pathology and Veterinary Diagnostics, Faculty of Veterinary Medicine, Warsaw University of Life Sciences-SGGW, Warsaw, Poland.
Cywińska, Anna
  • Department of Pathology and Veterinary Diagnostics, Faculty of Veterinary Medicine, Warsaw University of Life Sciences-SGGW, Warsaw, Poland.
Czopowicz, Michał
  • Laboratory of Veterinary Epidemiology and Economics, Faculty of Veterinary Medicine, Warsaw University of Life Sciences-SGGW, Warsaw, Poland.
Witkowski, Lucjan
  • Laboratory of Veterinary Epidemiology and Economics, Faculty of Veterinary Medicine, Warsaw University of Life Sciences-SGGW, Warsaw, Poland.
Niedźwiedź, Artur
  • Department of Internal Diseases with Clinic for Horses, Dogs and Cats, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life Sciences, Wrocław, Poland.
Słowikowska, Malwina
  • Department of Internal Diseases with Clinic for Horses, Dogs and Cats, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life Sciences, Wrocław, Poland.
Borowicz, Hieronim
  • Department of Internal Diseases with Clinic for Horses, Dogs and Cats, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life Sciences, Wrocław, Poland.
Jaśkiewicz, Anna
  • Department of Pathology and Veterinary Diagnostics, Faculty of Veterinary Medicine, Warsaw University of Life Sciences-SGGW, Warsaw, Poland.
Winnicka, Anna
  • Department of Pathology and Veterinary Diagnostics, Faculty of Veterinary Medicine, Warsaw University of Life Sciences-SGGW, Warsaw, Poland.

MeSH Terms

  • Animals
  • Case-Control Studies
  • Horse Diseases / blood
  • Horses
  • Musculoskeletal Diseases / veterinary
  • Serum Amyloid A Protein
  • Wounds and Injuries / veterinary

Conflict of Interest Statement

Competing Interests: The authors have declared that no competing interests exist.

References

This article includes 48 references
  1. Gabay C, Kushner I. Acute-phase proteins and other systemic responses to inflammation.. N Engl J Med 1999;340: 448–454.
    pubmed: 9971870
  2. Cray C. Acute phase proteins in animals. Progress in Molecular Biology and Translational Science 1st ed. Elsevier Inc.; 2012.
    pmc: PMC7149966pubmed: 22137431
  3. Jacobsen S, Andersen PH. The acute phase protein serum amyloid A (SAA) as a marker of inflammation in horses.. Equine Vet Educ 2007;19: 38–46.
  4. Belgrave RL, Dickey MM, Arheart KL, Cray C. Assessment of serum amyloid A testing of horses and its clinical application in a specialized equine practice.. J Am Vet Med Assoc 2013;243: 113–9.
    doi: 10.2460/javma.243.1.113pubmed: 23786199google scholar: lookup
  5. Canisso IF, Ball BA, Cray C, Williams NM, Scoggin KE, Davolli GM. Serum amyloid A and haptoglobin concentrations are increased in plasma of mares with ascending placentitis in the absence of changes in peripheral leukocyte counts or fibrinogen concentration.. Am J Reprod Immunol 2014;72: 378–385.
    pubmed: 24916762
  6. Pihl TH, Scheepers E, Sanz M, Goddard A, Page P, Toft N. Influence of disease process and duration on acute phase proteins in serum and peritoneal fluid of horses with colic.. J Vet Intern Med 2015;29: 651–658.
    doi: 10.1111/jvim.12542pmc: PMC4895517pubmed: 25644457google scholar: lookup
  7. Jacobsen S, Jensen JC, Frei S, Jensen AL, Thoefner MB. Use of serum amyloid A and other acute phase reactants to monitor the inflammatory response after castration in horses: a field study.. Equine Vet J 2005;37: 552–556.
    pubmed: 16295934
  8. Hobo S, Niwa H, Anzai T. Evaluation of serum amyloid A and surfactant protein D in sera for identification of the clinical condition of horses with bacterial pneumonia.. J Vet Med Sci 2007;69: 827–830.
    pubmed: 17827889
  9. Dyson PK, Jackson BF, Pfeiffer DU, Price JS. Days lost from training by two- and three-year-old Thoroughbred horses: a survey of seven UK training yards.. Equine Vet J 2008;40: 650–657.
    pubmed: 19165934
  10. Jackson BF, Lonnell C, Verheyen KLP, Dyson P, Pfeiffer DU, Price JS. Biochemical markers of bone metabolism and risk of dorsal metacarpal disease in 2-year-old Thoroughbreds.. Equine Vet J 2005;37: 87–91.
    pubmed: 15651741
  11. Jackson BF, Dyson PK, Lonnell C, Verheyen KLP, Pfeiffer DU, Price JS. Bone biomarkers and risk of fracture in two- and three-year-old Thoroughbreds.. Equine Vet J 2009;41: 410–413.
    pubmed: 19562906
  12. Verwilghen D, Busoni V, Gangl M, Franck T, Lejeune JP, Vanderheyden L. Relationship between biochemical markers and radiographic scores in the evaluation of the osteoarticular status of Warmblood stallions.. Res Vet Sci 2009;87: 319–328.
    doi: 10.1016/j.rvsc.2009.02.002pubmed: 19298987google scholar: lookup
  13. Turlo A, Cywinska A, Czopowicz M, Witkowski L, Szarska E, Winnicka A. Post-exercise dynamics of serum amyloid A blood concentration in thoroughbred horses classified as injured and non-injured after the race.. Res Vet Sci 2015;100: 223–225.
    doi: 10.1016/j.rvsc.2015.04.008pubmed: 25933933google scholar: lookup
  14. Cywińska A, Szarska E, Górecka R, Witkowski L, Hecold M, Bereznowski A. Acute phase protein concentrations after limited distance and long distance endurance rides in horses.. Res Vet Sci 2012;93: 1402–1406.
    doi: 10.1016/j.rvsc.2012.02.008pubmed: 22390917google scholar: lookup
  15. Cywinska A, Witkowski L, Szarska E, Schollenberger A, Winnicka A. Serum amyloid A (SAA) concentration after training sessions in Arabian race and endurance horses.. BMC Vet Res 2013;9: 91.
    doi: 10.1186/1746-6148-9-91pmc: PMC3655847pubmed: 23634727google scholar: lookup
  16. Pollock PJ, Prendergast M, Schumacher J, Bellenger CR. Effects of surgery on the acute phase response in clinically normal and diseased horses.. Vet Rec 2005;156: 538–542.
    pubmed: 15849343
  17. EL-Deeb WM, El-Bahr SM. Selected biochemical indicators of equine rhabdomyolysis in arabian horses: acute phase proteins and trace elements.. J Equine Vet Sci 2014;34: 484–488.
  18. Zar JH. Biostatistical analysis. 5th ed. Upper Saddle River: Prentice Hall; 2010.
  19. Hinchcliff KW, Kaneps AJ, Raymond JG. Equine sports medicine and surgery. St. Louis: Elsevier Ltd; 2004.
  20. Wilsher S, Allen WR, Wood JLN. Factors associated with failure of thoroughbred horses to train and race.. Equine Vet J 2006;38: 113–118.
    pubmed: 16536379
  21. Vandenplas ML, Moore JN, Barton MH, Ruossel AJ, Cohen ND. Concentrations of serum amyloid A and lipopolysaccharide-binding protein in horses with colic.. Am J Vet Res 2005;66: 1509–1516.
    pubmed: 16261823
  22. El-Deeb WM, El-Bahr SM. Investigation of selected biochemical indicators of equine rhabdomyolysis in arabian horses: pro-inflammatory cytokines and oxidative stress markers.. Vet Res Commun 2010;34: 677–689.
    doi: 10.1007/s11259-010-9439-5pubmed: 20830520google scholar: lookup
  23. Jonsdottir IH, Schjerling P, Ostrowski K, Asp S, Richter EA, Pedersen BK. Muscle contractions induce interleukin-6 mRNA production in rat skeletal muscles.. J Physiol 2000;528 Pt 1: 157–163.
    pmc: PMC2270126pubmed: 11018114
  24. Steensberg A, van Hall G, Osada T, Sacchetti M, Saltin B, Klarlund Pedersen B. Production of interleukin-6 in contracting human skeletal muscles can account for the exercise-induced increase in plasma interleukin-6.. J Physiol 2000;529: 237–242.
    pmc: PMC2270169pubmed: 11080265
  25. Liburt NR, Adams AA, Betancourt A, Horohov DW, McKeever KH. Exercise-induced increases in inflammatory cytokines in muscle and blood of horses.. Equine Vet J 2010;42: 280–288.
    pubmed: 21059019
  26. Lamprecht ED, Bagnell CA, Williams CA. Inflammatory responses to three modes of intense exercise in Standardbred mares–a pilot study.. Comp Exerc Physiol 2009;5: 115.
  27. Donovan DC, Jackson CA, Colahan PT, Norton N, Hurley DJ. Exercise-induced alterations in pro-inflammatory cytokines and prostaglandin F2α in horses.. Vet Immunol Immunopathol 2007;118: 263–269.
    pubmed: 17617470
  28. Pedersen BK, Steensberg A, Fischer C, Keller C, Ostrowski K, Schjerling P. Exercise and cytokines with particular focus on muscle-derived IL-6.. Exerc Immunol Rev 2001;7: 18–31.
    pubmed: 11579746
  29. Louis E, Raue U, Yang Y, Jemiolo B, Trappe S. Time course of proteolytic, cytokine, and myostatin gene expression after acute exercise in human skeletal muscle.. J Appl Physiol 2007;103: 1744–1751.
    pubmed: 17823296
  30. Nieman DC, Henson DA, Smith LL, Utter AC, Vinci DM, Davis JM. Cytokine changes after a marathon race.. J Appl Physiol (1985) 2001;91: 109–114.
    pubmed: 11408420
  31. Horohov DW, Sinatra ST, Chopra RK, Jankowitz S, Betancourt A, Bloomer RJ. The effect of exercise and nutritional supplementation on proinflammatory cytokine expression in young racehorses during training.. J Equine Vet Sci 2012;32: 805–815.
  32. Nieman DC, Dumke CL, Henson DA, McAnulty SR, Gross SJ, Lind RH. Muscle damage is linked to cytokine changes following a 160-km race.. Brain Behav Immun 2005;19: 398–403.
    pubmed: 16061149
  33. Millar NL, Wei AQ, Molloy TJ, Bonar F, Murrell GAC. Cytokines and apoptosis in supraspinatus tendinopathy.. 2009;91: 417–424.
    pubmed: 19258623
  34. Millar NL, Reilly JH, Kerr SC, Campbell AL, Little KJ, Leach WJ. Hypoxia: a critical regulator of early human tendinopathy.. 2012; 302–310.
    pubmed: 21972243
  35. John T, Lodka D, Kohl B, Ertel W, Jammrath J, Conrad C. Effect of pro-inflammatory and immunoregulatory cytokines on human tenocytes.. J Orthop Res 2010;28: 1071–1077.
    doi: 10.1002/jor.21079pubmed: 20127972google scholar: lookup
  36. Berglund M, Hart DA, Wiig M. The inflammatory response and hyaluronan synthases in the rabbit flexor tendon and tendon sheath following injury.. J Hand Surg Eur Vol 2007;32: 581–587.
    pubmed: 17950228
  37. Hosaka Y, Kirisawa R, Yamamoto E, Ueda H, Iwai H, Takehana K. Localization of cytokines in tendinocytes of the superficial digital flexor tendon in the horse.. J Vet Med Sci 2002;64: 945–947.
    pubmed: 12419874
  38. Langberg H, Olesen JL, Gemmer C, Kjaer M. Substantial elevation of interleukin-6 concentration in peritendinous tissue, in contrast to muscle, following prolonged exercise in humans.. J Physiol 2002;542: 985–990.
    pmc: PMC2290459pubmed: 12154195
  39. Jacobsen S, Niewold TA, Halling-Thomsen M, Nanni S, Olsen E, Lindegaard C. Serum amyloid A isoforms in serum and synovial fluid in horses with lipopolysaccharide-induced arthritis.. Vet Immunol Immunopathol 2006;110: 325–330.
    pubmed: 16337010
  40. Hultén C, Grönlund U, Hirvonen J, Tulamo R- M, Suominen MM, Marhaug G. Dynamics in serum of the inflammatory markers serum amyloid A (SAA), haptoglobin, fibrinogen and alpha2-globulins during induced noninfectious arthritis in the horse.. Equine Vet J 2002;34: 699–704.
    pubmed: 12455841
  41. Jacobsen S, Thomsen MH, Nanni S. Concentrations of serum amyloid A in serum and synovial fluid from healthy horses and horses with joint disease.. Am J Vet Res 2006;67: 1738–1742.
    pubmed: 17014325
  42. Bauzá G, Miller G, Kaseje N, Wigner NA, Wang Z, Gerstenfeld LC. The effects of injury magnitude on the kinetics of the acute phase response.. J Trauma 2011;70: 948–953.
    doi: 10.1097/TA.0b013e3181e1d27bpmc: PMC3563334pubmed: 20693926google scholar: lookup
  43. Buttenschoen K, Fleischmann W, Haupt U, Kinzl L, Buttenschoen DC. Translocation of endotoxin and acute-phase proteins in malleolar fractures.. J Trauma 2000;48: 241–245.
    pubmed: 10697081
  44. Yoon SI, Lim SS, Rha JD, Kim YH, Kang JS, Baek GH. The C-reactive protein (CRP) in patients with long bone fractures and after arthroplasty.. Int Orthop 1993;17: 198–201.
    pubmed: 8340178
  45. Billinghurst RC, Fretz PB, Gordon JR. Induction of intra-articular tumour necrosis factor during acute inflammatory responses in equine arthritis.. Equine Vet J 1995;27: 208–216.
    pubmed: 7556048
  46. Ley C, Ekman S, Elmén A, Nilsson G, Eloranta ML. Interleukin-6 and tumour necrosis factor in synovial fluid from horses with carpal joint pathology.. J Vet Med A Physiol Pathol Clin Med 2007;54: 346–351.
    pubmed: 17718806
  47. Campbell MD, Bellamy JE, Searcy GP. Determination of plasma fibrinogen concentration in the horse.. Am J Vet Res 1981;42: 100–104.
    pubmed: 7224302
  48. Jacobsen S, Nielsen JV, Kjelgaard-Hansen M, Toelboell T, Fjeldborg J, Halling-Thomsen M. Acute phase response to surgery of varying intensity in horses: a preliminary study.. Vet Surg 2009;38: 762–769.
    doi: 10.1111/j.1532-950X.2009.00564.xpubmed: 19674420google scholar: lookup

Citations

This article has been cited 7 times.
  1. Ayodele BA, Pagel CN, Mackie EJ, Armour F, Yamada S, Zahra P, Courtman N, Whitton RC, Hitchens PL. Differences in bone turnover markers and injury risks between local and international horses: A Victorian Spring Racing Carnival study. Equine Vet J 2025 Mar;57(2):333-346.
    doi: 10.1111/evj.14098pubmed: 38634210google scholar: lookup
  2. Merritt EK, Nieman DC, Toone BR, Groen A, Pugachev A. Proteomic Markers of Non-functional Overreaching During the Race Across America (RAAM): A Case Study. Front Physiol 2019;10:1410.
    doi: 10.3389/fphys.2019.01410pubmed: 31798466google scholar: lookup
  3. Turlo AJ, Cywinska A, Frisbie DD. Revisiting predictive biomarkers of musculoskeletal injury in thoroughbred racehorses: longitudinal study in polish population. BMC Vet Res 2019 Feb 26;15(1):66.
    doi: 10.1186/s12917-019-1799-7pubmed: 30808359google scholar: lookup
  4. Konstandi M, Sotiropoulos I, Matsubara T, Malliou F, Katsogridaki A, Andriopoulou CE, Gonzalez FJ. Adrenoceptor-stimulated inflammatory response in stress-induced serum amyloid A synthesis. Psychopharmacology (Berl) 2019 Jun;236(6):1687-1699.
    doi: 10.1007/s00213-018-5149-4pubmed: 30612190google scholar: lookup
  5. Witkowska-Piłaszewicz OD, Żmigrodzka M, Winnicka A, Miśkiewicz A, Strzelec K, Cywińska A. Serum amyloid A in equine health and disease. Equine Vet J 2019 May;51(3):293-298.
    doi: 10.1111/evj.13062pubmed: 30565319google scholar: lookup
  6. Ibrahim NI, Ahmad MS, Zulfarina MS, Zaris SNASM, Mohamed IN, Mohamed N, Mokhtar SA, Shuid AN. Activities of Daily Living and Determinant Factors among Older Adult Subjects with Lower Body Fracture after Discharge from Hospital: A Prospective Study. Int J Environ Res Public Health 2018 May 16;15(5).
    doi: 10.3390/ijerph15051002pubmed: 29772744google scholar: lookup
  7. Tozaki T, Kikuchi M, Kakoi H, Hirota KI, Mukai K, Aida H, Nakamura S, Nagata SI. Profiling of exercise-induced transcripts in the peripheral blood cells of Thoroughbred horses. J Equine Sci 2016;27(4):157-164.
    doi: 10.1294/jes.27.157pubmed: 27974875google scholar: lookup
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