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Home / Equine Research Bank / Vet Surg / The effect of repeated freezing and thawing on the suture pu...
Veterinary surgery : VS2022; 51(7); 1106-1110; doi: 10.1111/vsu.13855

The effect of repeated freezing and thawing on the suture pull-out strength in equine arytenoid and cricoid cartilages.

Abstract: To assess the effect of repeated freezing and thawing on the suture pull-out strength in arytenoid and cricoid cartilages subjected to the laryngoplasty (LP) procedure. Methods: Ex vivo experimental study. Methods: Ten grossly normal equine cadaveric larynges. Methods: Bilateral LP constructs were created using a standard LP technique. One hemilarynx was randomly allocated to the single freeze and thaw group and the other allocated to the repeated freeze and thaw (3 complete cycles) group. The suture ends of each LP construct were attached to a load frame and subjected to monotonic loading until construct failure. Mean load (N) and displacement (mm) at LP construct failure were compared between groups. Results: All LP constructs failed by suture pull through the arytenoid cartilage. The mean load at failure was similar between groups (118.9 ± 25.5 N in the single freeze and thaw group and 113.4 ± 20.5 N in the repeated freeze and thaw group, P = .62). The mean displacement at failure was similar between groups (54.4 ± 15.1 mm in the single freeze and thaw group and 54.4 ± 15.4 mm in the repeated freeze and thaw group, P = .99). Conclusions: Repeated freezing and thawing did not affect the suture pullout strength of the arytenoid and cricoid cartilages. Conclusions: Laryngeal specimens that have been subjected to repeated freezing and thawing can be utilized in the experimental evaluation of LP procedures because there is no alteration of the suture pull-out strength of the relevant cartilages.
© 2022 The Authors. Veterinary Surgery published by Wiley Periodicals LLC on behalf of American College of Veterinary Surgeons.
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Publication Date: 2022-07-11 PubMed ID: 35815735PubMed Central: PMC9796672DOI: 10.1111/vsu.13855Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article studies the impact of repeated freezing and thawing on the strength of sutures in horse’s arytenoid and cricoid cartilages during a laryngoplasty procedure. The results show that repeated freeze-thaw cycles do not weaken the suture’s holding power.

Methodology

  • The researchers carried out their experiment using ten healthy horse larynges obtained from cadavers.
  • They created laryngoplasty constructs on both sides of each larynx using a standard technique.
  • For each larynx, one side was subjected to a single freeze-thaw cycle, while the other side endured three full freeze-thaw cycles.
  • After these procedures, the ends of the sutures from each construct were attached to a load frame. This setup was then subjected to continuous loading until the construct failed.

Findings

  • The study’s results showed that all laryngoplasty constructs failed due to the sutures pulling through the arytenoid cartilage.
  • The average force at failure, represented as load in Newtons (N), was not significantly different between the two groups. The single freeze-thaw group had a mean load at failure of 118.9 ± 25.5 N, while the repeated freeze-thaw group had a mean load at failure of 113.4 ± 20.5 N. This difference was not statistically significant.
  • Similarly, the average displacement at failure, in millimeters (mm), was almost the same for both groups, with the single freeze-thaw group experiencing a mean displacement at failure of 54.4 ± 15.1 mm and the repeated freeze-thaw group showing a displacement at failure of 54.4 ± 15.4 mm.

Conclusions

  • These observations led the researchers to conclude that repeated freezing and thawing did not impact suture pull-out strength in arytenoid and cricoid cartilages.
  • Therefore, laryngeal specimens that have been subjected to repeated freeze-thaw cycles can be used in experimental evaluations of laryngoplasty procedures without worrying about changes to suture pull-out strength in the cartilages involved.

Cite This Article

APA
Gray SM, Gutierrez-Nibeyro SD, Horn GP, McCoy AM, Schaeffer DJ, Stewart M. (2022). The effect of repeated freezing and thawing on the suture pull-out strength in equine arytenoid and cricoid cartilages. Vet Surg, 51(7), 1106-1110. https://doi.org/10.1111/vsu.13855

Publication

Veterinary surgery : VS
ISSN: 1532-950X
NlmUniqueID: 8113214
Country: United States
Language: English
Volume: 51
Issue: 7
Pages: 1106-1110

Researcher Affiliations

Gray, Sarah M
  • Department of Clinical Veterinary Medicine, College of Veterinary Medicine, University of Illinois, Urbana-Champaign, Illinois, USA.
Gutierrez-Nibeyro, Santiago D
  • Department of Clinical Veterinary Medicine, College of Veterinary Medicine, University of Illinois, Urbana-Champaign, Illinois, USA.
Horn, Gavin P
  • Illinois Fire Service Institute, University of Illinois, Champaign, Illinois, USA.
McCoy, Annette M
  • Department of Clinical Veterinary Medicine, College of Veterinary Medicine, University of Illinois, Urbana-Champaign, Illinois, USA.
Schaeffer, David J
  • Department of Clinical Veterinary Medicine, College of Veterinary Medicine, University of Illinois, Urbana-Champaign, Illinois, USA.
Stewart, Matt
  • Department of Clinical Veterinary Medicine, College of Veterinary Medicine, University of Illinois, Urbana-Champaign, Illinois, USA.

MeSH Terms

  • Animals
  • Arytenoid Cartilage / surgery
  • Cadaver
  • Cricoid Cartilage / surgery
  • Freezing
  • Horses / surgery
  • Laryngoplasty / methods
  • Laryngoplasty / veterinary
  • Sutures / veterinary

Grant Funding

  • Project No: ILLU-888-347 / U.S. Department of Agriculture

Conflict of Interest Statement

The authors declare no conflicts of interest related to this report.

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Citations

This article has been cited 1 times.
  1. Tucker ML, Wilson DG, Bergstrom DJ, Carmalt JL. Computational fluid dynamic analysis of upper airway procedures in equine larynges.. Front Vet Sci 2023;10:1139398.
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