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Archives animal breeding2025; 68(1); 27-41; doi: 10.5194/aab-68-27-2025

The effect of resveratrol on the cryopreservation of Mongolian horse semen.

Abstract: Cryopreservation of semen has advanced significantly with the development of artificial insemination techniques, but post-thawed sperm often exhibit reduced viability, membrane integrity, and acrosome integrity compared to fresh sperm, leading to decreased fertilization capacity. Oxidative stress is a major concern during cryopreservation. This study investigated the use of resveratrol (RSV), a potent antioxidant, in the cryopreservation of Mongolian horse semen. Different concentrations of RSV were incorporated into semen cryopreservation extenders, and the morphological and antioxidant indices of post-thawed sperm were assessed to determine the optimal RSV concentration. The study also employed tandem mass tag (TMT) quantitative proteomics technology to explore differential proteins and their pathways. The results showed that sperm quality parameters were positively correlated with RSV concentration within a certain range (10-40 mol L) and were significantly higher than the control group. RSV also enhanced the antioxidant capacity of sperm, with the optimal effect observed at 40 mol L. Proteomics analysis identified 10 differential proteins between the control and optimal RSV concentration groups, with 7 upregulated proteins primarily involved in antioxidant activity and maintaining intracellular redox balance. These findings were further validated through real-time fluorescent quantitative PCR and protein immunoblotting, suggesting that RSV has potential as an effective antioxidant for improving the cryopreservation of Mongolian horse semen.
Copyright: © 2025 Ming Du et al.
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Publication Date: 2025-01-06 PubMed ID: 42232634PubMed Central: PMC13225121DOI: 10.5194/aab-68-27-2025Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

Overview

  • This study examined how resveratrol, an antioxidant, affects the quality and antioxidant status of Mongolian horse semen after cryopreservation, aiming to improve sperm survival and function.
  • The research identified an optimal resveratrol concentration that enhances sperm quality and antioxidant defenses, supported by proteomic and molecular analyses.

Background and Purpose

  • Cryopreservation (freezing) of semen is crucial for artificial insemination but often damages sperm, reducing their viability and fertilization ability.
  • Damage is largely caused by oxidative stress during the freezing and thawing process, leading to compromised membrane and acrosome integrity.
  • Resveratrol (RSV), a compound known for its strong antioxidant properties, was tested as an additive in semen extenders to mitigate oxidative stress and improve post-thaw sperm quality.

Methodology

  • Different concentrations of RSV (ranging from 10 to 40 micromolar, μmol/L) were added to the semen cryopreservation extenders.
  • Samples were frozen and then thawed to evaluate sperm morphological parameters including viability, membrane integrity, and acrosome integrity.
  • Antioxidant indices were measured to assess the antioxidant capacity of sperm with and without RSV treatment.
  • Tandem Mass Tag (TMT) quantitative proteomics was used to identify proteins differentially expressed due to RSV treatment, providing insight into molecular pathways involved.
  • Real-time fluorescent quantitative PCR and protein immunoblotting verified the proteomics results at the gene and protein expression levels.

Key Findings

  • Sperm quality parameters (viability, membrane and acrosome integrity) improved in a dose-dependent manner with RSV concentrations from 10 to 40 μmol/L compared to control (no RSV).
  • The optimal concentration was identified as 40 μmol/L where the sperm antioxidant capacity was significantly enhanced.
  • Proteomic analysis revealed 10 proteins that were differentially expressed between control and 40 μmol/L RSV groups.
  • Among these, 7 proteins were upregulated and mainly involved in antioxidant activity and maintaining intracellular redox balance, indicating a strengthened defense system against oxidative stress.
  • Validation by PCR and immunoblotting confirmed the expression patterns of these key proteins.

Implications

  • The study demonstrates that incorporating resveratrol into semen extenders can significantly improve post-thaw sperm quality by enhancing antioxidant defenses.
  • The identification of specific proteins involved in the antioxidant response provides molecular targets for further research on semen preservation.
  • Resveratrol shows promise as an effective additive to improve cryopreservation outcomes for Mongolian horse semen, potentially increasing fertility success rates in artificial insemination programs.
  • These findings could contribute to better preservation protocols that reduce oxidative damage during freezing and thawing processes in equine reproduction.

Cite This Article

APA
Du M, Liu Y, Zhang L, Li X, Wang N, He Q, Cao J, Zhao B, Shi Y, Li B, Bou G, Dugarjaviin M. (2025). The effect of resveratrol on the cryopreservation of Mongolian horse semen. Arch Anim Breed, 68(1), 27-41. https://doi.org/10.5194/aab-68-27-2025

Publication

Archives animal breeding
ISSN: 2363-9822
NlmUniqueID: 101701238
Country: Germany
Language: English
Volume: 68
Issue: 1
Pages: 27-41

Researcher Affiliations

Du, Ming
  • Key Laboratory of Equus Germplasm Innovation, Ministry of Agriculture and Rural Affairs, Hohhot 010018, China.
  • Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Inner Mongolia Agricultural University, Hohhot 010018, China.
  • Equus Research Center, Inner Mongolia Agricultural University, Hohhot 010018, China.
Liu, Yuanyi
  • Key Laboratory of Equus Germplasm Innovation, Ministry of Agriculture and Rural Affairs, Hohhot 010018, China.
  • Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Inner Mongolia Agricultural University, Hohhot 010018, China.
  • Equus Research Center, Inner Mongolia Agricultural University, Hohhot 010018, China.
Zhang, Lei
  • Key Laboratory of Equus Germplasm Innovation, Ministry of Agriculture and Rural Affairs, Hohhot 010018, China.
  • Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Inner Mongolia Agricultural University, Hohhot 010018, China.
  • Equus Research Center, Inner Mongolia Agricultural University, Hohhot 010018, China.
Li, Xinyu
  • Key Laboratory of Equus Germplasm Innovation, Ministry of Agriculture and Rural Affairs, Hohhot 010018, China.
  • Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Inner Mongolia Agricultural University, Hohhot 010018, China.
  • Equus Research Center, Inner Mongolia Agricultural University, Hohhot 010018, China.
Wang, Na
  • Key Laboratory of Equus Germplasm Innovation, Ministry of Agriculture and Rural Affairs, Hohhot 010018, China.
  • Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Inner Mongolia Agricultural University, Hohhot 010018, China.
  • Equus Research Center, Inner Mongolia Agricultural University, Hohhot 010018, China.
He, Qianqian
  • Key Laboratory of Equus Germplasm Innovation, Ministry of Agriculture and Rural Affairs, Hohhot 010018, China.
  • Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Inner Mongolia Agricultural University, Hohhot 010018, China.
  • Equus Research Center, Inner Mongolia Agricultural University, Hohhot 010018, China.
Cao, Jialong
  • Key Laboratory of Equus Germplasm Innovation, Ministry of Agriculture and Rural Affairs, Hohhot 010018, China.
  • Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Inner Mongolia Agricultural University, Hohhot 010018, China.
  • Equus Research Center, Inner Mongolia Agricultural University, Hohhot 010018, China.
Zhao, Bilig
  • Key Laboratory of Equus Germplasm Innovation, Ministry of Agriculture and Rural Affairs, Hohhot 010018, China.
  • Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Inner Mongolia Agricultural University, Hohhot 010018, China.
  • Equus Research Center, Inner Mongolia Agricultural University, Hohhot 010018, China.
Shi, Yujie
  • Key Laboratory of Equus Germplasm Innovation, Ministry of Agriculture and Rural Affairs, Hohhot 010018, China.
  • Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Inner Mongolia Agricultural University, Hohhot 010018, China.
  • Equus Research Center, Inner Mongolia Agricultural University, Hohhot 010018, China.
Li, Bei
  • Key Laboratory of Equus Germplasm Innovation, Ministry of Agriculture and Rural Affairs, Hohhot 010018, China.
  • Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Inner Mongolia Agricultural University, Hohhot 010018, China.
  • Equus Research Center, Inner Mongolia Agricultural University, Hohhot 010018, China.
Bou, Gerelchimeg
  • Key Laboratory of Equus Germplasm Innovation, Ministry of Agriculture and Rural Affairs, Hohhot 010018, China.
  • Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Inner Mongolia Agricultural University, Hohhot 010018, China.
  • Equus Research Center, Inner Mongolia Agricultural University, Hohhot 010018, China.
Dugarjaviin, Manglai
  • Key Laboratory of Equus Germplasm Innovation, Ministry of Agriculture and Rural Affairs, Hohhot 010018, China.
  • Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Inner Mongolia Agricultural University, Hohhot 010018, China.
  • Equus Research Center, Inner Mongolia Agricultural University, Hohhot 010018, China.

Conflict of Interest Statement

The contact author has declared that none of the authors has any competing interests.

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