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Home / Equine Research Bank / ScientificWorldJournal / The effectiveness of anti-R. equi hyperimmune plasma against...
TheScientificWorldJournal2014; 2014; 480732; doi: 10.1155/2014/480732

The effectiveness of anti-R. equi hyperimmune plasma against R. equi challenge in thoroughbred Arabian foals of mares vaccinated with R. equi vaccine.

Abstract: This study aimed to determine the effectiveness of a pregnant mare immunization of a Rhodococcus equi (R. equi) vaccine candidate containing a water-based nanoparticle mineral oil adjuvanted (Montanide IMS 3012) inactive bacterin and virulence-associated protein A (VapA), as well as the administration of anti-R. equi hyperimmune (HI) plasma against R. equi challenge in the mares' foals. The efficacy of passive immunizations (colostral passive immunity by mare vaccination and artificial passive immunity by HI plasma administration) was evaluated based on clinical signs, complete blood count, blood gas analysis, serological response (ELISA), interleukin-4 (IL-4) and interferon gamma (IFN- γ ), total cell count of the bronchoalveolar lavage fluids (BALF) samples, reisolation rate of R. equi from BALF samples (CFU/mL), lung samples (CFU/gr), and lesion scores of the organs and tissue according to pathological findings after necropsy in the foals. The vaccination of pregnant mares and HI plasma administration in the foals reduced the severity of R. equi pneumonia and lesion scores of the organs and tissue by 3.54-fold compared to the control foals. This study thus indicates that immunization of pregnant mares with R. equi vaccine candidate and administration of HI plasma in mares' foals effectively protect foals against R. equi challenge.
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Publication Date: 2014-04-03 PubMed ID: 24982958PubMed Central: PMC3997159DOI: 10.1155/2014/480732Google Scholar: Lookup
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study investigates the potential of a Rhodococcus equi vaccine and hyperimmune plasma in reducing the severity of R. equi pneumonia and organ and tissue damage in the foals of vaccinated mares.

Objective of the Study

  • The main objective of this research was to determine the effectiveness of an immunization protocol among pregnant mares. This protocol consisted of a vaccine candidate for Rhodococcus equi, a bacteria causing severe pneumonia in foals, and the administration of anti-R. equi hyperimmune plasma to the mares’ foals. The expectation was that these measures would protect the newborn foals from R. equi.

Methodology

  • The mitigatory potential of two passive immunizations was evaluated. One involved colostral passive immunity that resulted from the vaccination of the mare. The other involved artificial passive immunity induced by the administration of HI plasma.
  • Evaluation parameters included a range of clinical signs, complete blood count data, blood gas analysis results, serological response (measured via an ELISA test), interleukin-4 and interferon gamma levels, total cell count from the sample bronchoalveolar lavage fluids, the reisolation rate of R. equi, and the severity of organ and tissue lesions found after the foal underwent a necropsy.

Results and Conclusion

  • Vaccination of the pregnant mares and the administration of HI plasma to the foals had a beneficial effect. The severity of R. equi pneumonia and the extent of organ and tissue lesions were reduced by about 3.54-fold compared to the degree of illness and damage seen in control foals.
  • The results of this study show that inoculating pregnant mares with the R. equi vaccine prototype and administering HI plasma in mares’ foals can provide effective protection to foals from R. equi infection.

Cite This Article

APA
Erganis O, Sayin Z, Hadimli HH, Sakmanoglu A, Pinarkara Y, Ozdemir O, Maden M. (2014). The effectiveness of anti-R. equi hyperimmune plasma against R. equi challenge in thoroughbred Arabian foals of mares vaccinated with R. equi vaccine. ScientificWorldJournal, 2014, 480732. https://doi.org/10.1155/2014/480732

Publication

TheScientificWorldJournal
ISSN: 1537-744X
NlmUniqueID: 101131163
Country: United States
Language: English
Volume: 2014
Pages: 480732
PII: 480732

Researcher Affiliations

Erganis, Osman
  • Department of Microbiology, Faculty of Veterinary Medicine, University of Selcuk, 42075 Konya, Turkey.
Sayin, Zafer
  • Department of Microbiology, Faculty of Veterinary Medicine, University of Selcuk, 42075 Konya, Turkey.
Hadimli, Hasan Huseyin
  • Department of Microbiology, Faculty of Veterinary Medicine, University of Selcuk, 42075 Konya, Turkey.
Sakmanoglu, Asli
  • Department of Microbiology, Faculty of Veterinary Medicine, University of Selcuk, 42075 Konya, Turkey.
Pinarkara, Yasemin
  • Program of Food Technology, Sarayonu Vocational School, University of Selcuk, 42075 Konya, Turkey.
Ozdemir, Ozgur
  • Department of Pathology, Faculty of Veterinary Medicine, University of Selcuk, 42075 Konya, Turkey.
Maden, Mehmet
  • Department of Internal Medicine, Faculty of Veterinary Medicine, University of Selcuk, 42075 Konya, Turkey.

MeSH Terms

  • Actinomycetales Infections / immunology
  • Actinomycetales Infections / prevention & control
  • Animals
  • Antibodies, Bacterial / administration & dosage
  • Antibodies, Bacterial / blood
  • Female
  • Horses
  • Rhodococcus equi / immunology
  • Rhodococcus equi / pathogenicity
  • Vaccination

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Citations

This article has been cited 7 times.
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