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Journal of anatomy2009; 215(6); 682-691; doi: 10.1111/j.1469-7580.2009.01149.x

The elastin network: its relationship with collagen and cells in articular cartilage as visualized by multiphoton microscopy.

Abstract: A combination of two-photon fluorescence (TPF), second harmonic generation (SHG) and coherent anti-Stokes Raman scattering (CARS) imaging has been used to investigate the elastin fibre network in healthy equine articular cartilage from the metacarpophalangeal joint. The elastin fibres were identified using their intrinsic two-photon fluorescence and immuno-staining was used to confirm the identity of these fibres. SHG was used to reveal the collagen matrix and the collagen fibre orientations were determined from their SHG polarization sensitivity, while CARS was used to clearly delineate the cell boundaries. Extensive elastin fibre networks were found in all the joint regions investigated. The elastin was found predominantly in the superficial zone (upper 50 microm) and was aligned parallel to the articular surface. Elastin was also detected in the pericellular matrix surrounding the superficial chondrocytes; however, individual fibres could not be resolved in this region. Variations in the density and organization of the fibres were observed in different regions on the joint surface.
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Publication Date: 2009-10-01 PubMed ID: 19796069PubMed Central: PMC2796791DOI: 10.1111/j.1469-7580.2009.01149.xGoogle Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigates the relationship between the elastin fiber network, collagen, and cells in articular cartilage using various imaging techniques. In the study, they found extensive elastin fiber networks throughout all joint areas studied, noting that the elastin was mostly in the superficial zone and aligned parallel to the articular surface.

Methodology

  • Scientists used a combination of imaging techniques: Two-photon fluorescence (TPF), Second harmonic generation (SHG), and Coherent anti-Stokes Raman scattering (CARS) imaging, to investigate the elastin fibre network.
  • The specimens used for the research were the healthy equine articular cartilage from the metacarpophalangeal joint (a joint in the lower part of the forelimbs of horses).
  • Elastin fibers were identified through their intrinsic two-photon fluorescence and immuno-staining was employed to confirm the identity of these fibers.
  • SHG was used to unveil the collagen matrix, and the orientations of collagen fibers were determined using the SHG polarization sensitivity.
  • CARS was used to delineate the cell boundaries clearly.

Findings

  • The study found extensive elastin fiber networks in all the joint regions investigated.
  • Elastin, a protein that enables tissues in the body to resume their shape after stretching or contracting, was found predominantly in the superficial zone (the uppermost 50 micrometers) and aligned parallel to the articular surface.
  • Elastin was also detected in the pericellular matrix, the material surrounding the superficial chondrocytes (cartilage cells), but individual fibers in this region couldn’t be finely resolved.
  • There were observed variations in the density and organization of the fibers in different regions on the joint surface.

Implications

  • This research provides valuable insights into the organization of the elastin fiber network and its relationship with collagen and cells in articular cartilage.
  • The outcome of this study could potentially contribute to the understanding of the biomechanics of the joint, aid in creating accurate models of joint behavior, and even in the therapeutic development for joint diseases.
  • These findings could be particularly useful in biomedical research, in areas like orthopedics and rheumatology, where understanding the structural components of joints is crucial.

Cite This Article

APA
Mansfield J, Yu J, Attenburrow D, Moger J, Tirlapur U, Urban J, Cui Z, Winlove P. (2009). The elastin network: its relationship with collagen and cells in articular cartilage as visualized by multiphoton microscopy. J Anat, 215(6), 682-691. https://doi.org/10.1111/j.1469-7580.2009.01149.x

Publication

Journal of anatomy
ISSN: 1469-7580
NlmUniqueID: 0137162
Country: England
Language: English
Volume: 215
Issue: 6
Pages: 682-691

Researcher Affiliations

Mansfield, Jessica
  • Biophysics, School of Physics, University of Exeter, Exeter EX4 4QL, UK. j.c.mansfield@exeter.ac.uk
Yu, Jing
    Attenburrow, Don
      Moger, Julian
        Tirlapur, Uday
          Urban, Jill
            Cui, Zhanfeng
              Winlove, Peter

                MeSH Terms

                • Animals
                • Cartilage, Articular / chemistry
                • Cartilage, Articular / cytology
                • Collagen / analysis
                • Elastin / analysis
                • Horses / anatomy & histology
                • Horses / metabolism
                • Microscopy, Fluorescence, Multiphoton

                Grant Funding

                • BB/DO14751/1 / Biotechnology and Biological Sciences Research Council

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