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Home / Equine Research Bank / J Anat / The elastin network: its relationship with collagen and cell...
Journal of anatomy2009; 215(6); 682-691; doi: 10.1111/j.1469-7580.2009.01149.x

The elastin network: its relationship with collagen and cells in articular cartilage as visualized by multiphoton microscopy.

Abstract: A combination of two-photon fluorescence (TPF), second harmonic generation (SHG) and coherent anti-Stokes Raman scattering (CARS) imaging has been used to investigate the elastin fibre network in healthy equine articular cartilage from the metacarpophalangeal joint. The elastin fibres were identified using their intrinsic two-photon fluorescence and immuno-staining was used to confirm the identity of these fibres. SHG was used to reveal the collagen matrix and the collagen fibre orientations were determined from their SHG polarization sensitivity, while CARS was used to clearly delineate the cell boundaries. Extensive elastin fibre networks were found in all the joint regions investigated. The elastin was found predominantly in the superficial zone (upper 50 microm) and was aligned parallel to the articular surface. Elastin was also detected in the pericellular matrix surrounding the superficial chondrocytes; however, individual fibres could not be resolved in this region. Variations in the density and organization of the fibres were observed in different regions on the joint surface.
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Publication Date: 2009-10-01 PubMed ID: 19796069PubMed Central: PMC2796791DOI: 10.1111/j.1469-7580.2009.01149.xGoogle Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigates the relationship between the elastin fiber network, collagen, and cells in articular cartilage using various imaging techniques. In the study, they found extensive elastin fiber networks throughout all joint areas studied, noting that the elastin was mostly in the superficial zone and aligned parallel to the articular surface.

Methodology

  • Scientists used a combination of imaging techniques: Two-photon fluorescence (TPF), Second harmonic generation (SHG), and Coherent anti-Stokes Raman scattering (CARS) imaging, to investigate the elastin fibre network.
  • The specimens used for the research were the healthy equine articular cartilage from the metacarpophalangeal joint (a joint in the lower part of the forelimbs of horses).
  • Elastin fibers were identified through their intrinsic two-photon fluorescence and immuno-staining was employed to confirm the identity of these fibers.
  • SHG was used to unveil the collagen matrix, and the orientations of collagen fibers were determined using the SHG polarization sensitivity.
  • CARS was used to delineate the cell boundaries clearly.

Findings

  • The study found extensive elastin fiber networks in all the joint regions investigated.
  • Elastin, a protein that enables tissues in the body to resume their shape after stretching or contracting, was found predominantly in the superficial zone (the uppermost 50 micrometers) and aligned parallel to the articular surface.
  • Elastin was also detected in the pericellular matrix, the material surrounding the superficial chondrocytes (cartilage cells), but individual fibers in this region couldn’t be finely resolved.
  • There were observed variations in the density and organization of the fibers in different regions on the joint surface.

Implications

  • This research provides valuable insights into the organization of the elastin fiber network and its relationship with collagen and cells in articular cartilage.
  • The outcome of this study could potentially contribute to the understanding of the biomechanics of the joint, aid in creating accurate models of joint behavior, and even in the therapeutic development for joint diseases.
  • These findings could be particularly useful in biomedical research, in areas like orthopedics and rheumatology, where understanding the structural components of joints is crucial.

Cite This Article

APA
Mansfield J, Yu J, Attenburrow D, Moger J, Tirlapur U, Urban J, Cui Z, Winlove P. (2009). The elastin network: its relationship with collagen and cells in articular cartilage as visualized by multiphoton microscopy. J Anat, 215(6), 682-691. https://doi.org/10.1111/j.1469-7580.2009.01149.x

Publication

Journal of anatomy
ISSN: 1469-7580
NlmUniqueID: 0137162
Country: England
Language: English
Volume: 215
Issue: 6
Pages: 682-691

Researcher Affiliations

Mansfield, Jessica
  • Biophysics, School of Physics, University of Exeter, Exeter EX4 4QL, UK. j.c.mansfield@exeter.ac.uk
Yu, Jing
    Attenburrow, Don
      Moger, Julian
        Tirlapur, Uday
          Urban, Jill
            Cui, Zhanfeng
              Winlove, Peter

                MeSH Terms

                • Animals
                • Cartilage, Articular / chemistry
                • Cartilage, Articular / cytology
                • Collagen / analysis
                • Elastin / analysis
                • Horses / anatomy & histology
                • Horses / metabolism
                • Microscopy, Fluorescence, Multiphoton

                Grant Funding

                • BB/DO14751/1 / Biotechnology and Biological Sciences Research Council

                References

                This article includes 42 references
                1. Benninghoff A. Form und Bau der Gelenkknorpel in ihren Beziehungen zur Funktion. II. Der Aufbau des Gelenkknorpels in seinen Beziehungen zur Funktion.. Z Zellforsch Mikrosk Anat 1925;2:783–862.
                2. Boulesteix T, Pena AM, Pagès N, Godeau G, Sauviat MP, Beaurepaire E, Schanne-Klein MC. Micrometer scale ex vivo multiphoton imaging of unstained arterial wall structure.. Cytometry A 2006 Jan;69(1):20-6.
                  pubmed: 16342114doi: 10.1002/cyto.a.20196google scholar: lookup
                3. Brama PA, Karssenberg D, Barneveld A, van Weeren PR. Contact areas and pressure distribution on the proximal articular surface of the proximal phalanx under sagittal plane loading.. Equine Vet J 2001 Jan;33(1):26-32.
                  pubmed: 11191606doi: 10.2746/042516401776767377google scholar: lookup
                4. Brockbank KG, MacLellan WR, Xie J, Hamm-Alvarez SF, Chen ZZ, Schenke-Layland K. Quantitative second harmonic generation imaging of cartilage damage.. Cell Tissue Bank 2008 Dec;9(4):299-307.
                  pubmed: 18431689doi: 10.1007/s10561-008-9070-7google scholar: lookup
                5. Broom ND, Ngo T, Tham E. Traversing the intact/fibrillated joint surface: a biomechanical interpretation.. J Anat 2005 Jan;206(1):55-67.
                  pmc: PMC1571455pubmed: 15679871doi: 10.1111/j.0021-8782.2005.00371.xgoogle scholar: lookup
                6. Cheng JX, Jia YK, Zheng G, Xie XS. Laser-scanning coherent anti-Stokes Raman scattering microscopy and applications to cell biology.. Biophys J 2002 Jul;83(1):502-9.
                  pmc: PMC1302164pubmed: 12080137doi: 10.1016/s0006-3495(02)75186-2google scholar: lookup
                7. Choi JB, Youn I, Cao L, Leddy HA, Gilchrist CL, Setton LA, Guilak F. Zonal changes in the three-dimensional morphology of the chondron under compression: the relationship among cellular, pericellular, and extracellular deformation in articular cartilage.. J Biomech 2007;40(12):2596-603.
                  pmc: PMC2265315pubmed: 17397851doi: 10.1016/j.jbiomech.2007.01.009google scholar: lookup
                8. Freund I, Deutsch M, Sprecher A. Connective tissue polarity. Optical second-harmonic microscopy, crossed-beam summation, and small-angle scattering in rat-tail tendon.. Biophys J 1986 Oct;50(4):693-712.
                  pmc: PMC1329848pubmed: 3779007doi: 10.1016/s0006-3495(86)83510-xgoogle scholar: lookup
                9. Fu L, Gu M. Fibre-optic nonlinear optical microscopy and endoscopy.. J Microsc 2007 Jun;226(Pt 3):195-206.
                  pubmed: 17535259doi: 10.1111/j.1365-2818.2007.01777.xgoogle scholar: lookup
                10. Fu L, Jain A, Cranfield C, Xie H, Gu M. Three-dimensional nonlinear optical endoscopy.. J Biomed Opt 2007 Jul-Aug;12(4):040501.
                  pubmed: 17867789doi: 10.1117/1.2756102google scholar: lookup
                11. Gachet D, Billard F, Sandeau N, Rigneault H. Coherent anti-Stokes Raman scattering (CARS) microscopy imaging at interfaces: evidence of interference effects.. Opt Express 2007 Aug 6;15(16):10408-20.
                  pubmed: 19547393doi: 10.1364/oe.15.010408google scholar: lookup
                12. Gibson GJ, Verner JJ, Nelson FR, Lin DL. Degradation of the cartilage collagen matrix associated with changes in chondrocytes in osteoarthrosis. Assessment by loss of background fluorescence and immunodetection of matrix components.. J Orthop Res 2001 Jan;19(1):33-42.
                  pubmed: 11332618doi: 10.1016/s0736-0266(00)00008-5google scholar: lookup
                13. Hesse I. The occurrence of elastic system fibres in the matrix of normal articular cartilage.. Cell Tissue Res 1987 Jun;248(3):589-93.
                  pubmed: 3607850doi: 10.1007/bf00216487google scholar: lookup
                14. Huang S, Heikal AA, Webb WW. Two-photon fluorescence spectroscopy and microscopy of NAD(P)H and flavoprotein.. Biophys J 2002 May;82(5):2811-25.
                  pmc: PMC1302068pubmed: 11964266doi: 10.1016/s0006-3495(02)75621-xgoogle scholar: lookup
                15. Jones CW, Smolinski D, Willers C, Yates PJ, Keogh A, Fick D, Kirk TB, Zheng MH. Laser scanning confocal arthroscopy of a fresh cadaveric knee joint.. Osteoarthritis Cartilage 2007 Dec;15(12):1388-96.
                  pubmed: 17618133doi: 10.1016/j.joca.2007.05.003google scholar: lookup
                16. König K, Schenke-Layland K, Riemann I, Stock UA. Multiphoton autofluorescence imaging of intratissue elastic fibers.. Biomaterials 2005 Feb;26(5):495-500.
                  pubmed: 15276357doi: 10.1016/j.biomaterials.2004.02.059google scholar: lookup
                17. Légaré F, Evans CL, Ganikhanov F, Xie XS. Towards CARS Endoscopy.. Opt Express 2006 May 15;14(10):4427-32.
                  pubmed: 19516594doi: 10.1364/oe.14.004427google scholar: lookup
                18. Mackay-Smith MP. Pathogenesis and pathology of equine osteoarthritis.. J Am Vet Med Assoc 1962;141:1246–1252.
                19. Maroudas A, Mizrahi J, Benaim E. A Study of Compressive Properties of Cartilage Using Unconfined Compression: Example of an Experimental and a Theoretical Approach.. London: Portland Press; 1995.
                20. Moger CJ, Barrett R, Bleuet P, Bradley DA, Ellis RE, Green EM, Knapp KM, Muthuvelu P, Winlove CP. Regional variations of collagen orientation in normal and diseased articular cartilage and subchondral bone determined using small angle X-ray scattering (SAXS).. Osteoarthritis Cartilage 2007 Jun;15(6):682-7.
                  pubmed: 17306566doi: 10.1016/j.joca.2006.12.006google scholar: lookup
                21. Montes GS. Structural biology of the fibres of the collagenous and elastic systems.. Cell Biol Int 1996 Jan;20(1):15-27.
                  pubmed: 8936403doi: 10.1006/cbir.1996.0004google scholar: lookup
                22. Mow VC, Holmes MH, Lai WM. Fluid transport and mechanical properties of articular cartilage: a review.. J Biomech 1984;17(5):377-94.
                  pubmed: 6376512doi: 10.1016/0021-9290(84)90031-9google scholar: lookup
                23. Naumann A, Dennis JE, Awadallah A, Carrino DA, Mansour JM, Kastenbauer E, Caplan AI. Immunochemical and mechanical characterization of cartilage subtypes in rabbit.. J Histochem Cytochem 2002 Aug;50(8):1049-58.
                  pubmed: 12133908doi: 10.1177/002215540205000807google scholar: lookup
                24. Palero JA, de Bruijn HS, van der Ploeg van den Heuvel A, Sterenborg HJ, Gerritsen HC. Spectrally resolved multiphoton imaging of in vivo and excised mouse skin tissues.. Biophys J 2007 Aug 1;93(3):992-1007.
                  pmc: PMC1913153pubmed: 17449667doi: 10.1529/biophysj.106.099457google scholar: lookup
                25. Poole CA. Articular cartilage chondrons: form, function and failure.. J Anat 1997 Jul;191 ( Pt 1)(Pt 1):1-13.
                  pmc: PMC1467653pubmed: 9279653doi: 10.1046/j.1469-7580.1997.19110001.xgoogle scholar: lookup
                26. Poole CA, Flint MH, Beaumont BW. Chondrons in cartilage: ultrastructural analysis of the pericellular microenvironment in adult human articular cartilages.. J Orthop Res 1987;5(4):509-22.
                  pubmed: 3681525doi: 10.1002/jor.1100050406google scholar: lookup
                27. Roth S, Freund I. Second harmonic generation in collagen.. J Chem Phys 1978;70:1637–1643.
                28. Roth V, Mow V, Grodzinsky AJ. Biophysical and electromechanical properties of articular cartilage.. In: Simmons DJ, Kunin AS, editors. Skeletal Research: An experimental Approach. New York: Academic Press; 1979. pp. 301–341.
                29. Schenke-Layland K, Riemann I, Opitz F, König K, Halbhuber KJ, Stock UA. Comparative study of cellular and extracellular matrix composition of native and tissue engineered heart valves.. Matrix Biol 2004 May;23(2):113-25.
                  pubmed: 15246110doi: 10.1016/j.matbio.2004.03.005google scholar: lookup
                30. Smith LJ, Byers S, Costi JJ, Fazzalari NL. Elastic fibers enhance the mechanical integrity of the human lumbar anulus fibrosus in the radial direction.. Ann Biomed Eng 2008 Feb;36(2):214-23.
                  pubmed: 18066662doi: 10.1007/s10439-007-9421-8google scholar: lookup
                31. So PTC. Two-photon microscopy of tissues.. In: Mycek M-A, Pogue BW, editors. Handbook of Biomedical Fluorescence. New York: Marcel Dekker; 2003. pp. 181–209.
                32. So PT, Dong CY, Masters BR, Berland KM. Two-photon excitation fluorescence microscopy.. Annu Rev Biomed Eng 2000;2:399-429.
                  pubmed: 11701518doi: 10.1146/annurev.bioeng.2.1.399google scholar: lookup
                33. Stockwell RA. Biology of Cartilage Cells.. Cambridge, UK: Cambridge University Press; 1979.
                34. Stoller P, Kim BM, Rubenchik AM, Reiser KM, Da Silva LB. Polarization-dependent optical second-harmonic imaging of a rat-tail tendon.. J Biomed Opt 2002 Apr;7(2):205-14.
                  pubmed: 11966305doi: 10.1117/1.1431967google scholar: lookup
                35. Stoller P, Reiser KM, Celliers PM, Rubenchik AM. Polarization-modulated second harmonic generation in collagen.. Biophys J 2002 Jun;82(6):3330-42.
                  pmc: PMC1302120pubmed: 12023255doi: 10.1016/s0006-3495(02)75673-7google scholar: lookup
                36. Stoller P, Celliers PM, Reiser KM, Rubenchik AM. Quantitative second-harmonic generation microscopy in collagen.. Appl Opt 2003 Sep 1;42(25):5209-19.
                  pubmed: 12962402doi: 10.1364/ao.42.005209google scholar: lookup
                37. Tirlapur UK, Mulholland WJ, Bellhouse BJ, Kendall M, Cornhill JF, Cui Z. Femtosecond two-photon high-resolution 3D imaging, spatial-volume rendering and microspectral characterization of immunolocalized MHC-II and mLangerin/CD207 antigens in the mouse epidermis.. Microsc Res Tech 2006 Oct;69(10):767-75.
                  pubmed: 16941665doi: 10.1002/jemt.20331google scholar: lookup
                38. Yasui T, Tohno Y, Araki T. Determination of collagen fiber orientation in human tissue by use of polarization measurement of molecular second-harmonic-generation light.. Appl Opt 2004 May 10;43(14):2861-7.
                  pubmed: 15143809doi: 10.1364/ao.43.002861google scholar: lookup
                39. Yeh AT, Hammer-Wilson MJ, Van Sickle DC, Benton HP, Zoumi A, Tromberg BJ, Peavy GM. Nonlinear optical microscopy of articular cartilage.. Osteoarthritis Cartilage 2005 Apr;13(4):345-52.
                  pubmed: 15780648doi: 10.1016/j.joca.2004.12.007google scholar: lookup
                40. Yu J, Tirlapur U, Fairbank J, Handford P, Roberts S, Winlove CP, Cui Z, Urban J. Microfibrils, elastin fibres and collagen fibres in the human intervertebral disc and bovine tail disc.. J Anat 2007 Apr;210(4):460-71.
                  pmc: PMC2100288pubmed: 17428205doi: 10.1111/j.1469-7580.2007.00707.xgoogle scholar: lookup
                41. Yu J, Winlove PC, Roberts S, Urban JP. Elastic fibre organization in the intervertebral discs of the bovine tail.. J Anat 2002 Dec;201(6):465-75.
                  pmc: PMC1570996pubmed: 12489758doi: 10.1046/j.1469-7580.2002.00111.xgoogle scholar: lookup
                42. Zoumi A, Lu X, Kassab GS, Tromberg BJ. Imaging coronary artery microstructure using second-harmonic and two-photon fluorescence microscopy.. Biophys J 2004 Oct;87(4):2778-86.
                  pmc: PMC1304696pubmed: 15454469doi: 10.1529/biophysj.104.042887google scholar: lookup

                Citations

                This article has been cited 39 times.
                1. Al-Maslamani NA, Oldershaw R, Tew S, Curran J, D'Hooghe P, Yamamoto K, Horn HF. Chondrocyte De-Differentiation: Biophysical Cues to Nuclear Alterations.. Cells 2022 Dec 12;11(24).
                  doi: 10.3390/cells11244011pubmed: 36552775google scholar: lookup
                2. Boos MA, Lamandé SR, Stok KS. Multiscale Strain Transfer in Cartilage.. Front Cell Dev Biol 2022;10:795522.
                  doi: 10.3389/fcell.2022.795522pubmed: 35186920google scholar: lookup
                3. Sherlock BE, Chen J, Mansfield JC, Green E, Winlove CP. Biophotonic tools for probing extracellular matrix mechanics.. Matrix Biol Plus 2021 Dec;12:100093.
                  doi: 10.1016/j.mbplus.2021.100093pubmed: 34934939google scholar: lookup
                4. Wu JP, Yang X, Wang Y, Swift B, Adamson R, Zheng Y, Zhang R, Zhong W, Chen F. High Resolution and Labeling Free Studying the 3D Microstructure of the Pars Tensa-Annulus Unit of Mice.. Front Cell Dev Biol 2021;9:720383.
                  doi: 10.3389/fcell.2021.720383pubmed: 34692679google scholar: lookup
                5. Querido W, Kandel S, Pleshko N. Applications of Vibrational Spectroscopy for Analysis of Connective Tissues.. Molecules 2021 Feb 9;26(4).
                  doi: 10.3390/molecules26040922pubmed: 33572384google scholar: lookup
                6. Pinsard M, Laverty S, Richard H, Dubuc J, Schanne-Klein MC, Légaré F. Maturation of the Meniscal Collagen Structure Revealed by Polarization-Resolved and Directional Second Harmonic Generation Microscopy.. Sci Rep 2019 Dec 5;9(1):18448.
                  doi: 10.1038/s41598-019-54942-0pubmed: 31804577google scholar: lookup
                7. Lee JH, Rico-Jimenez JJ, Zhang C, Alex A, Chaney EJ, Barkalifa R, Spillman DR Jr, Marjanovic M, Arp Z, Hood SR, Boppart SA. Simultaneous label-free autofluorescence and multi-harmonic imaging reveals in vivo structural and metabolic changes in murine skin.. Biomed Opt Express 2019 Oct 1;10(10):5431-5444.
                  doi: 10.1364/BOE.10.005431pubmed: 31646056google scholar: lookup
                8. Jenkins TL, Little D. Synthetic scaffolds for musculoskeletal tissue engineering: cellular responses to fiber parameters.. NPJ Regen Med 2019;4:15.
                  doi: 10.1038/s41536-019-0076-5pubmed: 31263573google scholar: lookup
                9. Reed DA, Yotsuya M, Gubareva P, Toth PT, Bertagna A. Two-photon fluorescence and second harmonic generation characterization of extracellular matrix remodeling in post-injury murine temporomandibular joint osteoarthritis.. PLoS One 2019;14(3):e0214072.
                  doi: 10.1371/journal.pone.0214072pubmed: 30897138google scholar: lookup
                10. Feneck EM, Lewis PN, Ralphs J, Meek KM. A comparative study of the elastic fibre system within the mouse and human cornea.. Exp Eye Res 2018 Dec;177:35-44.
                  doi: 10.1016/j.exer.2018.07.024pubmed: 30053442google scholar: lookup
                11. Paquette ST, Dawes RP, Sundar IK, Rahman I, Brown EB, White PM. Chronic cigarette smoke exposure drives spiral ganglion neuron loss in mice.. Sci Rep 2018 Apr 10;8(1):5746.
                  doi: 10.1038/s41598-018-24166-9pubmed: 29636532google scholar: lookup
                12. Albro MB, Bergholt MS, St-Pierre JP, Vinals Guitart A, Zlotnick HM, Evita EG, Stevens MM. Raman spectroscopic imaging for quantification of depth-dependent and local heterogeneities in native and engineered cartilage.. NPJ Regen Med 2018;3:3.
                  doi: 10.1038/s41536-018-0042-7pubmed: 29449966google scholar: lookup
                13. Zyablitskaya M, Munteanu EL, Nagasaki T, Paik DC. Second Harmonic Generation Signals in Rabbit Sclera As a Tool for Evaluation of Therapeutic Tissue Cross-linking (TXL) for Myopia.. J Vis Exp 2018 Jan 6;(131).
                  doi: 10.3791/56385pubmed: 29364259google scholar: lookup
                14. Querido W, Falcon JM, Kandel S, Pleshko N. Vibrational spectroscopy and imaging: applications for tissue engineering.. Analyst 2017 Oct 23;142(21):4005-4017.
                  doi: 10.1039/c7an01055apubmed: 28956032google scholar: lookup
                15. Kim M, Farrell MJ, Steinberg DR, Burdick JA, Mauck RL. Enhanced nutrient transport improves the depth-dependent properties of tri-layered engineered cartilage constructs with zonal co-culture of chondrocytes and MSCs.. Acta Biomater 2017 Aug;58:1-11.
                  doi: 10.1016/j.actbio.2017.06.025pubmed: 28629894google scholar: lookup
                16. Bergholt MS, St-Pierre JP, Offeddu GS, Parmar PA, Albro MB, Puetzer JL, Oyen ML, Stevens MM. Raman Spectroscopy Reveals New Insights into the Zonal Organization of Native and Tissue-Engineered Articular Cartilage.. ACS Cent Sci 2016 Dec 28;2(12):885-895.
                  doi: 10.1021/acscentsci.6b00222pubmed: 28058277google scholar: lookup
                17. Zyablitskaya M, Takaoka A, Munteanu EL, Nagasaki T, Trokel SL, Paik DC. Evaluation of Therapeutic Tissue Crosslinking (TXL) for Myopia Using Second Harmonic Generation Signal Microscopy in Rabbit Sclera.. Invest Ophthalmol Vis Sci 2017 Jan 1;58(1):21-29.
                  doi: 10.1167/iovs.16-20241pubmed: 28055099google scholar: lookup
                18. Seeger M, Karlas A, Soliman D, Pelisek J, Ntziachristos V. Multimodal optoacoustic and multiphoton microscopy of human carotid atheroma.. Photoacoustics 2016 Sep;4(3):102-111.
                  doi: 10.1016/j.pacs.2016.07.001pubmed: 27761409google scholar: lookup
                19. Chen H, Zhao X, Berwick ZC, Krieger JF, Chambers S, Kassab GS. Microstructure and Mechanical Property of Glutaraldehyde-Treated Porcine Pulmonary Ligament.. J Biomech Eng 2016 Jun;138(6):061003.
                  doi: 10.1115/1.4033300pubmed: 27040732google scholar: lookup
                20. Novakofski KD, Pownder SL, Koff MF, Williams RM, Potter HG, Fortier LA. High-Resolution Methods for Diagnosing Cartilage Damage In Vivo.. Cartilage 2016 Jan;7(1):39-51.
                  doi: 10.1177/1947603515602307pubmed: 26958316google scholar: lookup
                21. Zhang C, Zhang D, Cheng JX. Coherent Raman Scattering Microscopy in Biology and Medicine.. Annu Rev Biomed Eng 2015;17:415-45.
                  doi: 10.1146/annurev-bioeng-071114-040554pubmed: 26514285google scholar: lookup
                22. Gilson RC, Tang R, Som A, Klajer C, Sarder P, Sudlow GP, Akers WJ, Achilefu S. Protonation and Trapping of a Small pH-Sensitive Near-Infrared Fluorescent Molecule in the Acidic Tumor Environment Delineate Diverse Tumors in Vivo.. Mol Pharm 2015 Dec 7;12(12):4237-46.
                  doi: 10.1021/acs.molpharmaceut.5b00430pubmed: 26488921google scholar: lookup
                23. Endres BT, Staruschenko A, Schulte M, Geurts AM, Palygin O. Two-photon Imaging of Intracellular Ca2+ Handling and Nitric Oxide Production in Endothelial and Smooth Muscle Cells of an Isolated Rat Aorta.. J Vis Exp 2015 Jun 10;(100):e52734.
                  doi: 10.3791/52734pubmed: 26132549google scholar: lookup
                24. Ross KA, Williams RM, Schnabel LV, Mohammed HO, Potter HG, Bradica G, Castiglione E, Pownder SL, Satchell PW, Saska RA, Fortier LA. Comparison of Three Methods to Quantify Repair Cartilage Collagen Orientation.. Cartilage 2013 Apr;4(2):111-20.
                  doi: 10.1177/1947603512461440pubmed: 26069654google scholar: lookup
                25. Kiyomatsu H, Oshima Y, Saitou T, Miyazaki T, Hikita A, Miura H, Iimura T, Imamura T. Quantitative SHG imaging in osteoarthritis model mice, implying a diagnostic application.. Biomed Opt Express 2015 Feb 1;6(2):405-20.
                  doi: 10.1364/BOE.6.000405pubmed: 25780732google scholar: lookup
                26. Chaudhary R, Campbell KR, Tilbury KB, Vanderby R Jr, Block WF, Kijowski R, Campagnola PJ. Articular cartilage zonal differentiation via 3D Second-Harmonic Generation imaging microscopy.. Connect Tissue Res 2015 Apr;56(2):76-86.
                  doi: 10.3109/03008207.2015.1013192pubmed: 25738523google scholar: lookup
                27. He B, Wu JP, Kirk TB, Carrino JA, Xiang C, Xu J. High-resolution measurements of the multilayer ultra-structure of articular cartilage and their translational potential.. Arthritis Res Ther 2014 Mar 12;16(2):205.
                  doi: 10.1186/ar4506pubmed: 24946278google scholar: lookup
                28. Chow MJ, Turcotte R, Lin CP, Zhang Y. Arterial extracellular matrix: a mechanobiological study of the contributions and interactions of elastin and collagen.. Biophys J 2014 Jun 17;106(12):2684-92.
                  doi: 10.1016/j.bpj.2014.05.014pubmed: 24940786google scholar: lookup
                29. Chen H, Zhao X, Lu X, Kassab G. Non-linear micromechanics of soft tissues.. Int J Non Linear Mech 2013 Nov;58:79-85.
                  doi: 10.1016/j.ijnonlinmec.2013.03.002pubmed: 24817769google scholar: lookup
                30. Green EM, Mansfield JC, Bell JS, Winlove CP. The structure and micromechanics of elastic tissue.. Interface Focus 2014 Apr 6;4(2):20130058.
                  doi: 10.1098/rsfs.2013.0058pubmed: 24748954google scholar: lookup
                31. Jutila AA, Zignego DL, Hwang BK, Hilmer JK, Hamerly T, Minor CA, Walk ST, June RK. Candidate mediators of chondrocyte mechanotransduction via targeted and untargeted metabolomic measurements.. Arch Biochem Biophys 2014 Mar 1;545:116-23.
                  doi: 10.1016/j.abb.2014.01.011pubmed: 24440608google scholar: lookup
                32. He B, Wu JP, Xu J, Day RE, Kirk TB. Microstructural and compositional features of the fibrous and hyaline cartilage on the medial tibial plateau imply a unique role for the hopping locomotion of kangaroo.. PLoS One 2013;8(9):e74303.
                  doi: 10.1371/journal.pone.0074303pubmed: 24058543google scholar: lookup
                33. Albro MB, Nims RJ, Cigan AD, Yeroushalmi KJ, Alliston T, Hung CT, Ateshian GA. Accumulation of exogenous activated TGF-β in the superficial zone of articular cartilage.. Biophys J 2013 Apr 16;104(8):1794-804.
                  doi: 10.1016/j.bpj.2013.02.052pubmed: 23601326google scholar: lookup
                34. Biela E, Galas J, Lee B, Johnson GL, Darzynkiewicz Z, Dobrucki JW. Col-F, a fluorescent probe for ex vivo confocal imaging of collagen and elastin in animal tissues.. Cytometry A 2013 Jun;83(6):533-9.
                  doi: 10.1002/cyto.a.22264pubmed: 23404939google scholar: lookup
                35. Mansfield JC, Winlove CP. A multi-modal multiphoton investigation of microstructure in the deep zone and calcified cartilage.. J Anat 2012 Apr;220(4):405-16.
                  doi: 10.1111/j.1469-7580.2012.01479.xpubmed: 22332832google scholar: lookup
                36. Takesono A, Moger J, Farooq S, Cartwright E, Dawid IB, Wilson SW, Kudoh T. Solute carrier family 3 member 2 (Slc3a2) controls yolk syncytial layer (YSL) formation by regulating microtubule networks in the zebrafish embryo.. Proc Natl Acad Sci U S A 2012 Feb 28;109(9):3371-6.
                  doi: 10.1073/pnas.1200642109pubmed: 22331904google scholar: lookup
                37. Chen H, Liu Y, Slipchenko MN, Zhao X, Cheng JX, Kassab GS. The layered structure of coronary adventitia under mechanical load.. Biophys J 2011 Dec 7;101(11):2555-62.
                  doi: 10.1016/j.bpj.2011.10.043pubmed: 22261042google scholar: lookup
                38. Arkill KP, Moger J, Winlove CP. The structure and mechanical properties of collecting lymphatic vessels: an investigation using multimodal nonlinear microscopy.. J Anat 2010 May;216(5):547-55.
                  doi: 10.1111/j.1469-7580.2010.01215.xpubmed: 20345855google scholar: lookup
                39. Yu J, Urban JP. The elastic network of articular cartilage: an immunohistochemical study of elastin fibres and microfibrils.. J Anat 2010 Apr;216(4):533-41.
                  doi: 10.1111/j.1469-7580.2009.01207.xpubmed: 20148992google scholar: lookup
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