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In vitro cellular & developmental biology. Animal2014; 50(6); 562-571; doi: 10.1007/s11626-013-9730-1

The influence of static magnetic fields on canine and equine mesenchymal stem cells derived from adipose tissue.

Abstract: The aim of this study was to evaluate the proliferation rate and morphological changes of adipose-derived mesenchymal stem cells of canine and equine origin (Eq- and CaAdMSC). Investigated cells were exposed to a static magnetic field (MF) with the intensity of 0.5 T. Proliferation activity of cells was determined with the Alamar Blue assay. Obtained results, normalized in respect to the control culture, showed that EqAdMSC exposed to MF maintained a high proliferation status, whereas proliferation activity of CaAdMSC cultured in the presence of MF was decreased. Estimations of population doubling time (PDT) also revealed that EqAdMSCs exposed to static MF achieved a twofold increase in the total number of cells in a shorter amount of time than the control culture. The PDT value obtained for investigated CaAdMSCs indicated that MF exposure resulted in the prolongation of population doubling time. Morphology of cells and cellular composition was investigated using a light inverted microscope and a fluorescent microscope. A scanning electron microscope was used for microvesicles (MVs) imaging. Obtained results showed that both cell types maintained fibroblastic morphology and did not reveal signs of apoptosis or necrosis. However, the MF had an influence on the MVs secretion. While EqAdMSCs propagated in the presence of MF were characterized by the abundant MVs presence, CaAdMSCs revealed poor secretory activity. The approach presented provides complex analysis, which enables one to determine changes in equine and canine cytophysiology.
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Publication Date: 2014-01-30 PubMed ID: 24477562PubMed Central: PMC4062816DOI: 10.1007/s11626-013-9730-1Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research aimed to determine the impact of static magnetic fields on the proliferation and morphology of stem cells derived from canine and equine adipose tissue. The study found distinct impact on the cells’ growth and development rate, as well as their secretion of microvesicles – tiny cellular segments that carry information for cell communication – differing between the two animal species.

Investigation Methodology

The researchers employed the following methods:

  • The study focused on canine and equine adipose-derived mesenchymal stem cells (Eq- and CaAdMSC).
  • These stem cells were exposed to a static magnetic field (MF) of 0.5 T intensity.
  • An Alamar Blue assay was used to determine the proliferation rates of the cells – essentially their ability to grow and multiply.
  • Morphological changes in these cells were studied using both light inverted microscopy and fluorescent microscopy.
  • To observe and image the presence of microvesicles (MVs), the researchers utilized a scanning electron microscope.

Main Findings

In exploring the findings:

  • The EqAdMSC preserved their high proliferation status when exposed to the magnetic field, while the CaAdMSC showed a decline in their proliferation when under the influence of the magnetic field.
  • Population Doubling Time (PDT) estimates indicated that under the influence of the MF, EqAdMSCs multiplied faster compared to the control group. In contrast, CaAdMSCs took longer to double their population.
  • Both cell types maintained their fibroblastic—thread-like—structure and showed no signs of apoptosis (scheduled cell death) or necrosis (untimely cell death), which are usual indications of cell healthiness and vitality.
  • The magnetic field influenced microvesicle secretion, which are vital to cell communication. EqAdMSCs showed abundant microvesicle presence when propagated with MF, while CaAdMSCs displayed poor secretory activity.

Implications

In conclusion, the research presents novel insights into the impact of magnetic fields on stem cells derived from different species. Particularly, it delineates the distinction in the response between canine and equine stem cells, suggesting species-specific reactions to magnetic fields. While the influence on equine cells seemed positive, promoting their growth and microvesicle production, the magnetic field had a contrary effect on the canine cells, decelerating their proliferation and limiting microvesicle secretion. This research provides a comprehensive understanding of changes incurred in cellular physiology due to exposure to magnetic fields, with potential implications in regenerative medicine and therapies involving stem cells.

Cite This Article

APA
Marędziak M, Marycz K, Smieszek A, Lewandowski D, Toker NY. (2014). The influence of static magnetic fields on canine and equine mesenchymal stem cells derived from adipose tissue. In Vitro Cell Dev Biol Anim, 50(6), 562-571. https://doi.org/10.1007/s11626-013-9730-1

Publication

In vitro cellular & developmental biology. Animal
ISSN: 1543-706X
NlmUniqueID: 9418515
Country: Germany
Language: English
Volume: 50
Issue: 6
Pages: 562-571

Researcher Affiliations

Marędziak, Monika
  • Electron Microscopy Laboratory, University of Environmental and Life Sciences Wroclaw, Kozuchowska 5b, 51-631, Wroclaw, Poland, monika.maredziak@gmail.com.
Marycz, Krzysztof
    Smieszek, Agnieszka
      Lewandowski, Daniel
        Toker, Nezir Yaşar

          MeSH Terms

          • Adipocytes / physiology
          • Adipocytes / radiation effects
          • Adipose Tissue / cytology
          • Adipose Tissue / radiation effects
          • Animals
          • Apoptosis / radiation effects
          • Cell Differentiation / radiation effects
          • Cell Proliferation / radiation effects
          • Dogs
          • Horses
          • Magnetic Fields / adverse effects
          • Mesenchymal Stem Cells / physiology
          • Mesenchymal Stem Cells / radiation effects

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